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Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

The effects of sodium gluconate and microbial phytase on performance and mineral utilisation in broiler chicks

M. Günal
+ Author Affiliations
- Author Affiliations

Department of Animal Science, Faculty of Agriculture, Suleyman Demirel University 32200, Isparta, Turkey. Email: mevlutgunal@sdu.edu.tr

Animal Production Science 53(4) 316-321 https://doi.org/10.1071/AN12221
Submitted: 22 June 2012  Accepted: 5 September 2012   Published: 14 January 2013

Abstract

An experiment was conducted to investigate the effects of sodium gluconate and microbial phytase (MP) (Natuphos 1000G) supplementation of diets on performance, mineral retention and bone mineralisation in male broiler chicks from a day old to 21 days of age. The experiment was carried out using a completely randomised design with a 3 by 2 factorial arrangement (0, 2 and 4% sodium gluconate and 0 and 750 U MP/kg diet). Diets were formulated with deficient contents of available phosphorus (aP) (2.4 g/kg). Ten replicate cages of four chicks per replicate cage were fed experimental diets. MP supplementation of diets with deficient contents of aP significantly improved weight gain (P < 0.05) and feed efficiency (P < 0.01). The performance results were similar in birds fed the diets without sodium gluconate and the diets with 2% sodium gluconate. However, the dietary inclusion of sodium gluconate at 4% depressed (P < 0.05) the growth of broiler chickens compared with the diet without sodium gluconate. Compared with the diets supplemented with 2% sodium gluconate, the diets supplemented with 4% sodium gluconate decreased (P < 0.05) weight gain and increased (P < 0.05) feed efficiency. MP supplementation increased (P < 0.01) Ca, P, Mg, Zn retention, and tibia ash, Ca and P contents in tibia ash. Diets with sodium gluconate increased (P < 0.05) P and Zn retention. Diets containing sodium gluconate without phytase increased (P < 0.05) Mg retention and P content in tibia ash. The diet with 2% sodium gluconate without phytase also increased (P < 0.05) Ca retention. The dietary inclusion of sodium gluconate at 2% improved (P < 0.05) tibia ash and Ca content in tibia ash. Sodium gluconate and MP had significant interactions in Ca and Mg retention (P < 0.01), and P content (P < 0.05) in tibia ash. In conclusion, the results indicated that phytase and sodium gluconate supplementation of corn–soybean meal low AP diets increased Ca, P, Mg and Zn mineral retention and bone mineralisation in chicks. The addition of MP improved the performance. However, the supplementation of diets with 4% sodium gluconate depressed the performance.


References

Ahmad T, Rasool S, Sarwar M, Haq A, Hasan Z (2000) Effect of microbial phytase produced from a fungus Aspergillus niger on bioavailability of phosphorus and calcium in broiler chickens. Animal Feed Science and Technology 83, 103–114.
Effect of microbial phytase produced from a fungus Aspergillus niger on bioavailability of phosphorus and calcium in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXosVajtQ%3D%3D&md5=c973aa88dcbe7d13c66c95a6b44e2418CAS |

AOAC (1990) ‘Official methods of analysis of the Association of Official Analytical Chemists.’ 15th edn. (Association of Official Analytical Chemists: Washington, DC)

Applegate TJ, Angel R, Classen HL (2003) Effect of dietary calcium, 25-hydroxycholecalciferol, or bird strain on small intestinal phytase activity in broiler chickens. Poultry Science 82, 1140–1148.

Asano T, Yuasa K, Kunugita K, Teraji T, Mitsuoka T (1994) Effects of sodium gluconate on human faecal bacteria. Microbial Ecology in Health and Disease 7, 247–256.
Effects of sodium gluconate on human faecal bacteria.Crossref | GoogleScholarGoogle Scholar |

Aureli R, Umar Faruk M, Cechova I, Pedersen PB, Elvig-Joergensen SG, Fru F, Broz J (2011) The efficacy of a novel microbial 6-phytase expressed in Aspergillus oryzae on the performance and phosphorus utilization in broiler chickens. International Journal of Poultry Science 10, 160–168.
The efficacy of a novel microbial 6-phytase expressed in Aspergillus oryzae on the performance and phosphorus utilization in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjt1Khtr8%3D&md5=017d1e4d4ca4aaee9db0d91ec9e13ce4CAS |

Biggs P, Parsons CM (2008) The effects of several organic acids on growth performance, nutrient digestibilities, and cecalmicrobial populations in young chicks. Poultry Science 87, 2581–2589.
The effects of several organic acids on growth performance, nutrient digestibilities, and cecalmicrobial populations in young chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXmtVemtg%3D%3D&md5=a596f5b9cef1a49e640e1e6cc33f2be9CAS |

Boling SD, Webel DM, Marromichalis I, Parsons CM, Baker DH (2000) The effect of citric acid on phytate phosphorus utilization in young ckicks and pigs. Journal of Animal Science 78, 682–689.

Brenes A, Viveros A, Arija I, Centeno C, Pizarro M, Braro C (2003) The effect of citric acid and microbial phytase on mineral utilization in broiler chicks. Animal Feed Science and Technology 110, 201–219.
The effect of citric acid and microbial phytase on mineral utilization in broiler chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXot1KjsL0%3D&md5=20c000f94f0244ee4f230d03af544473CAS |

Cheryan M (1980) Phytic acid interactions in food systems. CRC Critical Reviews in Food Science and Nutrition 13, 297–335.
Phytic acid interactions in food systems.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXivV2juw%3D%3D&md5=56afafe0759126b29adbcb3baed4019bCAS |

Ebrahimnezhad Y, Shivazad M, Taherkhani R, Nazeradl K (2008) Effects of ethylenediaminetetraacetic acid on phytate phosphorus utilization and effciency of microbial phytase in broiler chicks. Journal of Animal Physiology and Animal Nutrition 92, 168–172.
Effects of ethylenediaminetetraacetic acid on phytate phosphorus utilization and effciency of microbial phytase in broiler chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmtFGqtL0%3D&md5=9a80c963152eaddaae7592b740183bc8CAS |

Guo Y, Shi Y, Li F, Chen J, Zhen C, Hao Z (2009) Effects of sodium gluconate and phytase on performance and bone characteristics in broiler chickens. Animal Feed Science and Technology 150, 270–282.
Effects of sodium gluconate and phytase on performance and bone characteristics in broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXksVWgtrw%3D&md5=89ec9bb29f0aa8eff09cd501cde7703aCAS |

Keshavarz K (2000) Reevaluation of nonphytate phosphorus requirement of growing pullets with and without phytase. Poultry Science 79, 1143–1153.

Knuckles BE, Betschsrt AA (1987) Effect of phytate and other myo-inositol phosphate esters on alpha-amylase digestion of starch. Journal of Food Science 52, 719–721.
Effect of phytate and other myo-inositol phosphate esters on alpha-amylase digestion of starch.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2sXltFCguro%3D&md5=6526fdaa47a735806b927a1bea106779CAS |

Kornegay ET (2001) Digestion of phosphorus and other nutrients: the role of phytase and factors influencing their activity. In ‘Enzymes in farm animal nutrition’. (Eds MR Bedford, GG Partridge) pp. 237–272. (CABI: Wallingford, UK)

Kornegay ET, Denbow DM, Yi Z, Ravindran V (1996) Response of broilers to graded levels of microbial phytase added to maize–soyabean–meal diets containing three levels of nonphytate phosphorus of non-phytate phosphorus. The British Journal of Nutrition 75, 839–852.
Response of broilers to graded levels of microbial phytase added to maize–soyabean–meal diets containing three levels of nonphytate phosphorus of non-phytate phosphorus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XksFWgtL4%3D&md5=760855b6feb70b72d5769a79377b0ccdCAS |

Leeson S, Namkung H, Cottrill M, Forsberg CW (2000) Efficacy of new bacterial phytase in poultry diets. Canadian Journal of Animal Science 80, 527–528.
Efficacy of new bacterial phytase in poultry diets.Crossref | GoogleScholarGoogle Scholar |

Lim HS, Nanikung H, Um JS, Kang KR, Kim BS, Paik IK (2001) The effects of phytase supplementation on the performance of broiler chickens fed diets with different levels of non-phytin phosphorus. Asian-Australasian Journal of Animal Sciences 14, 250–257.

Luo HZ, Wu JL, Xu S, Lu YL, Xu YX, Xu SC (1997) The effect of supplemental phytase on growth performance and phosphorus utilization of broiler chickens. Acta Agric Zhejiang 9, 260–265.

Maenz DD, Engele-Schann CM, Newkirk RW, Classen HL (1999) The effect of minerals and mineral chelators on the formation of phytate-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal. Animal Feed Science and Technology 81, 177–192.
The effect of minerals and mineral chelators on the formation of phytate-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXmsVWmur4%3D&md5=aba3fe21ac2da29d70e5108842600ff0CAS |

Minitab (2000) ‘Minitab reference manual (release 13.0).’ (Minitab Inc.: State College, PA)

Mohanna C, Nys Y (1999) Changes in zinc and manganese availability in broiler chicks induced by vegetal and microbial phytases. Animal Feed Science and Technology 77, 241–253.
Changes in zinc and manganese availability in broiler chicks induced by vegetal and microbial phytases.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXpslWjsQ%3D%3D&md5=5c7b8947f0ae15ce51e2675699f57d2cCAS |

Orban JI, Adeola O, Stroshine R (1999) Microbial phytase in finisher diets of white pekin ducks: effect on growth performance, plasma phosphorus concentration, and leg bone characteristics. Poultry Science 78, 366–377.

Pallauf J, Hohler D, Rimbach G (1992) Effekt einer zulage an mikrobieller phytase zu einer mais-soja-diat auf die scheinbare absorption von Mg, Fe, Cu, Mn und Zn sowie auf parametter des zinkstatus beim ferkel. Journal of Animal Physiology and Animal Nutrition 68, 1–9.
Effekt einer zulage an mikrobieller phytase zu einer mais-soja-diat auf die scheinbare absorption von Mg, Fe, Cu, Mn und Zn sowie auf parametter des zinkstatus beim ferkel.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXitlartL8%3D&md5=d583298c991d44210a4864d6a072c1d4CAS |

Perney KM, Cantor AH, Straw ML, Herkelman KL (1993) The effect of dietary phytase on growth performance and phosphorus utilization of broiler chickens. Poultry Science 72, 2106–2114.
The effect of dietary phytase on growth performance and phosphorus utilization of broiler chickens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXntlGq&md5=1bd853a09b3a02f046c07ae2e0a1d671CAS |

Qian H, Kornegay ET, Denbow DM (1997) Utilization of phytate phosphorus and calcium as influenced by microbial phytase, cholecalciferol and the calcium:total phosphorus ratio in broiler diets. Poultry Science 76, 37–46.

Rafacz-Livingston KA, Parsons CM, Jungk RA (2005) The effects of various organic acids on phytate phosphorus utilization in chicks. Poultry Science 84, 1356–1362.

Rama Rao SV, Ravindra Reddy V, Ramasubba Reddy V (1999) Enhancement of phytate phosphorus availability in the diets of commercial broilers and layers. Animal Feed Science and Technology 79, 211–222.
Enhancement of phytate phosphorus availability in the diets of commercial broilers and layers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXivFeltrY%3D&md5=2be6c4cde3b7a63942016c040c25dab8CAS |

Ramachandran S, Fontanille P, Pandey A, Larroche C (2006) Gluconic acid: properties, applications and microbial production, a review. Food Technology and Biotechnology 44, 185–195.

Ravindran V, Kornegay ET (1993) Acidification of weaning pig diets: a review. Journal of the Science of Food and Agriculture 62, 313–322.
Acidification of weaning pig diets: a review.Crossref | GoogleScholarGoogle Scholar |

Ravindran V, Bryden WL, Kornegay ET (1995) Phytates: occurrences, bioavailability and implications in poultry nutrition. Poultry and Avian Biology Reviews 6, 125–143.

Ravindran V, Cabahug S, Ravindran G, Selle PH, Bryden WL (2000) Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. British Poultry Science 41, 193–200.
Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorus levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXksVCmtLc%3D&md5=80d2a9348de093da92d8979526a262afCAS |

Roberson KD, Edwards HM (1994) Effects of 1,25-dihydroxicholecalciferol and phytase on zinc utilization in broiler chicks. Poultry Science 73, 1312–1326.
Effects of 1,25-dihydroxicholecalciferol and phytase on zinc utilization in broiler chicks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXosVentA%3D%3D&md5=090f4be30ffc62e5f8914516dd9b0795CAS |

Sakata T, Adachi M, Hashida M, Sato N, Kojima T (1995) Effect of n-butyricacid on epithelial cell proliferation of pig colonic mucosa in short-term culture. DTW. Deutsche Tierarztliche Wochenschrift 102, 163–164.

Sebastian S, Touchburn SP, Chavez ER, Lague PC (1996) The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn–soyabean diets. Poultry Science 75, 729–736.
The effects of supplemental microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn–soyabean diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XksVKqurw%3D&md5=a8c56bf749194130f34fe1d8dbe92421CAS |

Selle PH, Ravindran V (2007) Microbial phytase in poultry nutrition. Animal Feed Science and Technology 135, 1–41.
Microbial phytase in poultry nutrition.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXkt1artb8%3D&md5=06cae9b25d8831416364251777c68850CAS |

Selle PH, Ravindran V, Caldwell RA, Bryden WL (2000) Phytate and phytase: consequences from protein utilization. Nutrition Research Reviews 13, 255–278.
Phytate and phytase: consequences from protein utilization.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXhtlKmtr8%3D&md5=b172a56cb0c57c84d9da038873609683CAS |

Simons PCM, Versteegh HAJ, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MGE, Beudeker R, Verschoor GJ (1990) Improvement of phosphorus availability by microbial phytase in broilers and pigs. The British Journal of Nutrition 64, 525–540.
Improvement of phosphorus availability by microbial phytase in broilers and pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXlslSrsrc%3D&md5=53d6a0e61c9d6dc566f03f64c7765a87CAS |

Singer M (2010) Acidifying sauces and dressings without compromising taste. Available at http://www.jungbunzlauer.com/media/uploads/pdf/Gluconates/AcidifyingsaucesanddressingsMar2010AR.pdf [Verified 22 August 2012]

Um JS, Paik IK (1999) Effects of microbial phytase supplementation on egg production, eggshell quality, and mineral retention of laying hens fed different levels of phosphorus. Poultry Science 78, 75–79.

Viveros A, Brenes A, Arija I, Centeno C (2002) Effects of microbial phytase supplementation on mineral utilization and serum enzyme activities in broiler chicks fed different levels of phosphorus. Poultry Science 81, 1172–1183.