The identity of the Depuch Island rock-wallaby revealed through ancient DNA

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Contents Vol 35(1)

doi:10.1071/AM11044

The Journal of the Australian Mammal Society

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The identity of the Depuch Island rock-wallaby revealed through ancient DNA

D. Haouchar ^A, J. Haile ^A, P. B. S. Spencer ^B and M. Bunce ^A ^C

^A Ancient DNA Laboratory, School of Biological Sciences, Murdoch University, Perth, WA 6150, Australia.
^B Wildlife Identification Laboratory, School of Biological Sciences, Murdoch University, Perth, WA 6150, Australia.
^C Corresponding author. Email: m.bunce@murdoch.edu.au

Australian Mammalogy 35(1) 101-106 http://dx.doi.org/10.1071/AM11044
Submitted: 21 November 2011 Accepted: 25 July 2012 Published: 26 October 2012





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Abstract

Ancient DNA is becoming increasingly recognised as a tool in conservation biology to audit past biodiversity. The widespread loss of Australian biodiversity, especially endemic mammal populations, is of critical concern. An extreme example occurred on Depuch Island, situated off the north-west coast of Western Australia, where an unidentified species of rock-wallaby (Petrogale sp.) became extinct as a result of predation by red foxes. Two potential candidate species, Petrogale lateralis and P. rothschildi, both have ranges adjacent to Depuch Island, making identification based on geography difficult. A museum bone (one of the only surviving Depuch Island specimens) was subjected to standard ancient DNA analyses and procedures. Mitochondrial DNA cytochrome b and hypervariable control region were targeted for species identification. Ancient DNA was successfully recovered from the bone: 200 base pairs (bp) of control region and 975 bp of the cytochrome b gene. Bayesian phylogenetic analyses were employed to model the Depuch Island rock-wallaby DNA sequences together with sequences of other rock-wallaby taxa from GenBank. Evidence suggests that of the two Petrogale lateralis subspecies proposed to have inhabited Depuch Island, Petrogale lateralis lateralis was identified as the most likely. The identification of the Depuch Island rock-wallaby population may assist in the reintroduction of an insurance population of Petrogale lateralis lateralis, which is becoming increasingly threatened on mainland Australia.

Additional keywords: Past biodiversity, Conservation.

References

Abbott, I. (2000). Improving the conservation of threatened and rare mammal species through translocation to islands: case study Western Australia. Biological Conservation 93, 195–201.
| CrossRef |

Burbidge, A. A., and Manly, B. F. J. (2002). Mammal extinctions on Australian islands: causes and conservation implications. Journal of Biogeography 29, 465–473.
| CrossRef |

Burbidge, A. A., Williams, M. R., and Abbott, I. (1997). Mammals of Australian islands: factors influencing species richness. Journal of Biogeography 24, 703–715.
| CrossRef |

Cooper, A., and Poinar, H. N. (2000). Ancient DNA: do it right or not at all. Science 289, 1139.
| CAS |

Eldridge, M. D. B., and Close, R. L. (1992). Taxonomy of rock wallabies, Petrogale (Marsupialia, Macropodidae). I. A revision of the eastern Petrogale with the description of three new species. Australian Journal of Zoology 40, 605–625.
| CrossRef |

Eldridge, M. D. B., and Close, R. L. (1997). Chromosomes and evolution in rock-wallabies, Petrogale (Marsupialia: Macropodidae). Australian Mammalogy 19, 123–135.

Eldridge, M. D. B., and Spencer, P. B. S. (1997). Sources and uses of genetic material in the study of Petrogale (rock wallabies) and other mammals. Australian Mammalogy 19, 265–278.

Eldridge, M. D. B., Close, R. L., and Johnston, P. G. (1991a). Chromosomal rearrangements in rock-wallabies, Petrogale (Marsupialia, Macropodidae). IV. G-Banding analysis of the Petrogale lateralis complex. Australian Journal of Zoology 39, 621–627.
| CrossRef |

Eldridge, M. D. B., Johnston, P. G., and Close, R. L. (1991b). Chromosomal rearrangements in rock-wallabies, Petrogale (Marsupialia, Macropodidae). V. Chromosomal phylogeny of the lateralis–penicillata group. Australian Journal of Zoology 39, 629–641.
| CrossRef |

Eldridge, M. D. B., Kinnear, J. E., and Close, R. L. (1994). Identification of rock wallabies in the Calvert ranges, Little Sandy Desert, Western Australia, as Petrogale lateralis lateralis. Australian Mammalogy 17, 129–131.

Eldridge, M. D. B., King, J. M., Loupis, A. K., Spencer, P. B. S., Taylor, A. C., Pope, L. C., and Hall, G. P. (1999). Unprecedented low levels of genetic variation and inbreeding depression in an island population of the black-footed rock-wallaby. Conservation Biology 13, 531–541.
| CrossRef |

Eldridge, M. D. B., Wilson, A. C. C., Metcalfe, C. J., Dollin, A. E., Bell, J. N., Johnson, P. M., Johnston, P. G., and Close, R. L. (2001). Taxonomy of rock-wallabies, Petrogale (Marsupialia: Macropodidae). III. Molecular data confirms the species status of the purple-necked rock-wallaby (Petrogale purpureicollis Le Souef). Australian Journal of Zoology 49, 323–344.
| CrossRef |

Frankham, R. (1997). Do island poulations have less genetic variation than mainland populations? Heredity 78, 311–327.
| CrossRef |

Frankham, R., Ballou, J., and Briscoe, D. A. (2002). ‘Introduction to Conservation Genetics.’ (Cambridge University Press: England.)

Hall, G. P., and Kinnear, J. E. (1991). Recovery plan for the black-flanked rock-wallaby Petrogale lateralis lateralis (Gould) (Australian National Parks and Wildlife Service Endangered Species Program, Project 149). Department of Conservation and Land Management, Western Australia.

Huelsenbeck, J. P., and Ronquist, F. (2001). Mr Bayes: Baysian inference of phylogenetic trees. Bioinformatics (Oxford, England) 17, 754–755.
| CrossRef | CAS |

Johnson, C. (2006). ‘Australia’s Mammal Extinctions: a 50 000 Year History.’ (Cambridge University Press: America.)

Johnson, C. N., and Isaac, J. L. (2009). Body mass and extinction risk in Australian marsupials: the ‘Critical Weight Range’ revisited. Austral Ecology 34, 35–40.
| CrossRef |

Kinnear, J. E., Onus, M. L., and Bromilow, R. N. (1988). Fox control and rock-wallaby population dynamics. Australian Wildlife Research 15, 435–450.
| CrossRef |

Kinnear, J. E., Onus, M. L., and Sumner, N. R. (1998). Fox control and rock-wallaby population dynamics – II. An update. Wildlife Research 25, 81–88.
| CrossRef |

Loupis, A. K., and Eldridge, M. D. B. (2001). Restriction fragment length polymorphism (RFLP) analysis of three nuclear genes in rock-wallabies (Petrogale: Marsupialia: Macropodidae): a search for genic markers to identify taxa within the Petrogale lateralis–penicillata group. Australian Journal of Zoology 49, 27–36.
| CrossRef |

Mason, R., Browning, T., and Eldridge, M. (2011). Reduced MHC class II diversity in island compared to mainland populations of the black-footed rock-wallaby (Petrogale lateralis lateralis). Conservation Genetics 12, 91–103.
| CrossRef |

Maxwell, S., Burbidge, A. A., and Morris, K. (1996). ‘Action plan for the Australian Marsupials and Monotremes’ for the Australasian Marsupial and Monotreme Specialist Group, IUCN Species Survival Commission. Wildlife Australia, Canberra.

McCarthy, F. D. (1961). The rock-engravings of Depuch Island, north-west Australia. Records of the Australian Museum 25, 121–148.
| CrossRef |

Nylander, J. A. A. (2004). MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala Univeristy.

Pearson, D. J., and Kinnear, J. E. (1997). A review of the distribution status and conservation of rock-wallabies in Western Australia. Australian Mammalogy 19, 137–152.

Péron, F. (1807). ‘A Voyage of Discovery to the Southern Hemisphere Performed by the Order of the Emperor Napolean during the Years 1801, 1802,1803, and 1804. (London.) pp. 129–132.

Potter, S., Cooper, S. J. B., Metcalfe, C. J., Taggart, D. A., and Eldridge, M. D. B. (2012). Phylogenetic relationships of rock-wallabies, Petrogale (Marsupialia: Macropodidae) and their biogeographic history within Australia. Molecular Phylogenetics and Evolution 62, 640–652.
| CrossRef |

Pruvost, M., and Geigl, E. M. (2004). Real-time quantitative PCR to assess the authenticity of ancient DNA amplification. Journal of Archaeological Science 31, 1191–1197.

Ride, W. D. L. (1964). Depuch Island. The Western Australian Museum 2, 1–89.

Sharman, G., Close, R., and Maynes, G. (1989). Chromosome evolution, phylogeny and speciation of rock wallabies (Petrogale, Macropodidae). Australian Journal of Zoology 37, 351–363.
| CrossRef |

Short, J., and Smith, A. (1994). Mammal decline and recovery in Australia. Journal of Mammalogy 75, 288–297.
| CrossRef |

Stokes, J. L. (1846). ‘Discoveries in Australia during the voyage of H.M.S. Beagle in the years 1837–38–39–40–41–42–43.’ (T. and W. Boone: London.)

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