Evaluation of green tea by-product and green tea plus probiotics on the growth performance, meat quality and immunity of growing–finishing pigs

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Contents Vol 52(9)

doi:10.1071/AN11141

Food, Fibre and Pharmaceuticals from Animals

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Evaluation of green tea by-product and green tea plus probiotics on the growth performance, meat quality and immunity of growing–finishing pigs

M. E. Hossain ^A, S. Y. Ko ^A, K. W. Park ^A, J. D. Firman ^B and C. J. Yang ^A ^C

^A Department of Animal Science and Technology, Sunchon National University, Suncheon, Jeonnam 540-742, South Korea.
^B Department of Animal Sciences, University of Missouri, Columbia, MO, USA.
^C Corresponding author. Email: yangcj@scnu.kr

Animal Production Science 52(9) 857-866 http://dx.doi.org/10.1071/AN11141
Submitted: 15 July 2011 Accepted: 7 March 2012 Published: 16 July 2012





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Abstract

The present study was conducted to evaluate the effects of green tea by-product (GTB) and green tea plus probiotics (GT+P) on the growth performance, carcass characteristics, meat quality, blood parameters and immunity of growing–finishing pigs. In total, 80 crossbreed growing pigs were assigned to receive four dietary treatments for a period of 8 weeks. The dietary treatments were a basal diet (control), basal diet supplemented with 0.003% chlortetracycline (antibiotic), basal diet with 0.5% GTB (GTB) and basal diet containing 0.5% GT+P (GT+P). The results of our study indicated that bodyweight gain increased (P < 0.05) in response to the addition of GT+P to the basal diet. Crude protein and crude ash content, and shear values of loin meat were significantly (P < 0.05) increased in the GT+P group, although moisture and juiciness were decreased (P < 0.05). The GTB group had higher (P < 0.05) serum glucose concentrations, whereas the GT+P exhibited lower (P < 0.05) insulin concentrations. The values of thiobarbituric acid-reactive substances of fresh loin meat and meat that had been preserved for 1 week were lower (P < 0.05) in the GT+P group than those of the control and GTB groups. The growth of spleen cells incubated in concanavalin A (Con A) and lipopolysaccharide (LPS) medium was statistically higher (P < 0.05) for the GT+P group than for the GTB or antibiotic group. IL-6 and TNF-α production by spleen cells induced by Con A and LPS was increased in the GTB and GT+P group (P < 0.05) compared with the antibiotic group. Taken together, the results of the present study indicated that GT+P exerts positive effects on weight gain, meat composition, blood parameters and immunity in pigs, and could be used as an alternative to antibiotics for growing–finishing pig feeds.

Additional keywords: blood parameters, carcass characteristics, carcass composition, IL-6, TBARS, TNF-α.

References

Akunda JK, Johnson E, Ahrens FA, Kramer T (2001) Chlorotetracycline modulates acute phase response of ex vivo perfused pig livers, and inhibits TNF-α secretion by isolated kupffer cells. Comparative Immunology, Microbiology and Infectious Diseases 24, 81–89.
| CrossRef | CAS |

Al-Harthi MA (2004) Efficiency of utilizing some spices and herbs with or without antibiotic supplementation on growth performance and carcass characteristics of broiler chicks. Egyptian Poultry Science Journal 24, 869–899.

Ali AA, Velasquez MT, Hansen CT, Mohamed AI, Bhathen SJ (2005) Modulation of carbohydrate metabolism and peptide hormones by soybean isoflavones and probiotics in obesity and diabetes. The Journal of Nutritional Biochemistry 16, 693–699.
| CrossRef | CAS |

Anjum MI, Khan AG, Azim A, Afzal M (2005) Effect of dietary supplementation of multi-strain probiotic on broiler growth performance. Pakistan Veterinary Journal 25, 25–29.

AOAC (2000) ‘Official methods of analysis.’ 17th edn. (Association of Official Analytical Chemists: Arlington, VA)

Biswas MAH, Wakita M (2001) Effect of dietary Japanese green tea powder supplementation on feed utilization and carcass profiles in broilers. Japanese Poultry Science 38, 50–57.
| CrossRef |

Brunnekreeft JW, Boerma GJM, Leijnse B (1983) Direct determination of total cholesterol by column gas chromatographic analysis without previous derivatisation compared with WHO–CDC reference method. Annals of Clinical Biochemistry 20, 360–363.

Bureenok S, Tamaki M, Kawamoto Y, Nakada T (2007) Additive effects of green tea on fermented juice of epiphytic lactic acid bacteria (FJLB) and the fermentative quality of rhodesgrass silage. Asian-Australasian Journal of Animal Sciences 20, 920–924.

Cao BH, Karasawa Y, Guo YM (2005) Effects of green tea polyphenols and fructo-oligosaccharides in semi-purified diets on broilers’ performance and caecal microflora and their metabolites. Asian-Australasian Journal of Animal Sciences 18, 85–89.

Cross DE, Mcdevitt RM, Hillman K, Acamovic T (2007) The effect of herbs and their associated essential oils on performance, dietary digestibility and gut microflora in chickens from 7 to 28 days of age. British Poultry Science 48, 496–506.
| CrossRef | CAS |

Cunningham HM (1971) Effect of caffeine on growth, feed efficiency and leanness of growing pigs and its interaction with calcium, zinc and corn oil. Canadian Journal of Animal Science 51, 95–102.
| CrossRef | CAS |

Elwakkad ASE, Al-azhary DB, Mohamed SA, Elzayat SR, Hebishy MA (2011) The enhancement of the anti inflammatory effect of caffeine on green tea extract and EGCG on obese rats. Journal of American Science 7, 128–138.

Ezekowitz RAB, Hoffmann J (1998) Innate immunity: the blossoming of innate immunity. Current Opinion in Immunology 10, 9–11.

Fuller R (1989) Probiotics in man and animals. The Journal of Applied Bacteriology 66, 365–378.
| CrossRef | CAS |

Giang HH, Viet TQ, Ogle B, Lindberg JE (2010) Growth performance, digestibility, gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria. Livestock Science 129, 95–103.
| CrossRef |

Hara H, Orita N, Hatano S, Ichikawa H, Hara Y, Matsumoto N, Kimura Y, Terada A, Mitsuoka T (1995) Effect of tea polyphenols on fecal flora and fecal metabolic products of pigs. The Journal of Veterinary Medical Science 57, 45–49.
| CrossRef | CAS |

Hara-Kudo Y, Yamasaki A, Sasaki M, Okubo T, Minai Y, Haga M, Kondo K, Sugita-Konishi Y (2005) Antibacterial action on pathogenic bacterial spore by green tea catechins. Journal of the Science of Food and Agriculture 85, 2354–2361.
| CrossRef | CAS |

Harold N, Graham PD (1992) Green tea composition, consumption and polyphenol chemistry. Journal of Preventive Medicine and Hygiene 21, 334–350.

Hazzit M, Baaliouamer A, Faleiro ML, Miguel MG (2006) Composition of the essential oils of thymus and origanum species from Algeria and their antioxidant and antimicrobial activities. Journal of Agricultural and Food Chemistry 54, 6314–6321.
| CrossRef | CAS |

Hu ZQ, Toda M, Okubo S, Hara Y, Shimamura T (1992) Mitogenic activity of (–) epigallocatechin gallate on B-cells and investigation of its structure function relationship. International Journal of Immunopharmacology 14, 1399–1407.
| CrossRef | CAS |

Ikeda I, Kobashi M, Hamada T, Tsuda K, Goto H, Imaizumi K, Nozawa A, Sugimoto A, Kakuda T (2003) Heat-epimerized tea catechins rich in gallocatechin gallate and catechin gallate are more effective to inhibit cholesterol absorption than tea catechins rich in epigallocatechin gallate and epicatechin gallate. Journal of Agricultural and Food Chemistry 51, 7303–7307.
| CrossRef | CAS |

Ishihara N, Chu DC, Akachiand S, Juneja LR (2001) Improvement of intestinal microflora balance and prevention of digestive and respiratory organ diseases in calves by green tea extracts. Livestock Production Science 68, 217–229.
| CrossRef |

Jansman AJM (1993) Tannins in feedstuffs for simple stomached animals. Nutrition Research Reviews 6, 209–236.
| CrossRef | CAS |

Jin SK, Kim IS, Song YM, Lee SD, Hah KH, Kim HY, Nam KY, Jang AR (2003) Effects of dietary biotite powder on physico-chemical characteristics of pork. Journal of Animal Science and Technology 45, 499–508.
| CrossRef |

Jin HH, Yang JL, Chung JH, Kim Y (2004) Hypocholesterolemic effects of green tea in cholesterol fed rats. Journal of the Korean Society of Food Science and Nutrition 33, 47–51.
| CrossRef | CAS |

Jun KD, Kim HJ, Lee KH, Paik HD, Kang JS (2002) Characterization of Bacillus polyfermenticus SCD as a probiotic. Korean Journal of Microbiology and Biotechnology 30, 359–366.

Kabir SML, Rahman MM, Rahman MB, Rahman MM, Ahmed SU (2004) The dynamics of probiotics on growth performance and immune response in broilers. International Journal of Poultry Science 3, 361–364.
| CrossRef |

Kalavathy R, Abdullah N, Jalaludin S, Ho YW (2003) Effects of lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. British Poultry Science 44, 139–144.
| CrossRef | CAS |

KAPE (2010) ‘Animal products grade system: the pork carcass grading.’ (Korea Institute for Animal Products Quality Evaluation: Gunpo, Korea). Available at http://www.ekape.or.kr/ view/ eng/system/pork.asp [verified 15 December 2011].

Karaca T, Yoruk M, Yoruk IH, Uslu S (2010) Effects of extract of green tea and ginseng on pancreatic beta cells and levels of serum glucose, insulin, cholesterol and triglycerides in rats with experimentally streptozotocin-induced diabetes: a histochemical and immunohistochemical study. Journal of Animal and Veterinary Advances 9, 102–107.
| CrossRef |

Khan AA, Slifer TR, Araujo FG, Suzuki Y, Remington JS (2000) Protection against lipopolysaccharide-induced death by fluoroquinolones. Antimicrobial Agents and Chemotherapy 44, 3169–3173.
| CrossRef | CAS |

Kim HY (2000) Hypocholesterolemic and hypotensive activities of Eucommia ulmoides oliver. Food Industry and Nutrition 5, 27–28.

Kim DW, Kim SH, Yu DJ, Kang GH, Kim JH, Kang HG, Jang BG, Na JC, Suh OS, Jang IS, Lee KS (2007) Effects of single or mixed supplements of plant extract, fermented medicinal plants and Lactobacillus on growth performance in broilers. Korean Journal of Poultry Science 34, 187–196.
| CrossRef | CAS |

Ko SY, Yang CJ (2008) Effect of green tea probiotics on the growth performance, meat quality and immune response in finishing pigs. Asian-Australasian Journal of Animal Sciences 21, 1339–1347.

Ko SY, Bae IH, Yee ST, Lee SS, Uuganbayar D, Oh JI, Yang CJ (2008) Comparison of the effect of green tea by-product and green tea probiotics on the growth performance, meat quality, and immune response of finishing pigs. Asian-Australasian Journal of Animal Sciences 21, 1486–1494.

Kondo M, Nakano M, Kaneko A, Agata H, Kita K, Yokota H (2004) Ensiled green tea waste as partial replacement for soybean meal and alfalfa hay in lactating cows. Asian-Australasian Journal of Animal Sciences 17, 960–966.

Korean Ministry for Food, Agriculture, Forestry and Fisheries (2008) ‘Guidelines for the care and use of animals in research.’ (Korean Ministry for Food, Agriculture, Forestry and Fisheries: Seoul, Korea)

Kyriakis SC, Tsiloyiannis VK, Vlemmas J, Sarris K, Tsinas AC, Alexopoulos C, Jansegers L (1999) The effect of probiotic LSP 122 on the control of post-weaning diarrhoea syndrome of piglets. Research in Veterinary Science 67, 223–228.
| CrossRef | CAS |

Lecomte A (1985) Green tea ‘Arkocaps’/Phytotrim^® double-blind trial clinical results. Revue de L’association Mondiale de Phytotherapie 1, 36–40.

Lee SG (2005) Effect of dietary green tea by-product on productivity in Korean beef cattle. MSc Thesis, Sunchon National University, Suncheon, South Korea.

Li S, Hacker RR (1995) The effect of caffeine on mammary gland development and milk yield in primiparous sows. Journal of Animal Science 73, 534–540.

Mountzouris KC, Tsirtsikos P, Kalamara E, Nitsch S, Schatzmayr G, Fegeros K (2007) Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus, and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poultry Science 86, 309–317.

Mukhtar H, Ahmad N (1999) Mechanism of cancer chemopreventive activity of green tea. Proceedings of the Society for Experimental Biology and Medicine 220, 234–238.
| CrossRef | CAS |

Nishida T, Eruden B, Hosoda K, Nakagawa K, Miyazawa T, Shioya S (2006) Effects of green tea (Camellia sinensis) waste silage and polyethyleneon ruminal fermentation and blood components in cattle. Asian-Australasian Journal of Animal Sciences 19, 1728–1736.

Nocek JE, Kautz WP, Leedle JA, Block E (2003) Direct-fed microbial supplementation on the performance of dairy cattle during the transition period. Journal of Dairy Science 86, 331–335.
| CrossRef | CAS |

NRC (1998) ‘Nutrient requirements of swine.’ 10th edn. (National Academy Press: Washington, DC)

Pae M, Ren Z, Meydani M, Shang F, Meydani SN, Wu D (2010) Epigallocatechin-3-gallate directly suppresses T cell proliferation through impaired IL-2 utilization and cell cycle progression. The Journal of Nutrition 140, 1509–1515.
| CrossRef | CAS |

Palacios R (1982) Concanavalin A triggers T lymphocytes by directly interacting with their receptors for activation. Journal of Immunology (Baltimore, MD.: 1950) 128, 337–342.

Pearson DA, Frankel EN, Aeschbach R, German JB (1998) Inhibition of endothelial cell mediated low-density lipoprotein oxidation by green tea extracts. Journal of Agricultural and Food Chemistry 46, 1445–1449.
| CrossRef | CAS |

Priyankarage N, Silva SSP, Gunaratne SP, Kothalawala H, Palliyaguru MWCD, Gunawardana GA (2003) Efficacy of probiotics and their effects on performance, carcase characteristics, intestinal microflora and Salmonella incidence in broilers. British Poultry Science 44, 26–27.
| CrossRef |

Sarker MSK, Ko SY, Lee SM, Kim GM, Choi JK, Yang CJ (2010a) Effect of different feed additives on growth performance and blood profiles of Korean Hanwoo calves. Asian–Australasian Journal of Animal Sciences 23, 52–60.

Sarker MSK, Yim KJ, Ko SY, Uuganbayar D, Kim GM, Bae IH, Oh JI, Yee ST, Yang CJ (2010b) Green tea level on growth performance and meat quality in finishing pigs. Pakistan Journal of Nutrition 9, 10–14.
| CrossRef | CAS |

Sarker MSK, Kim GM, Yang CJ (2010c) Effect of green tea and biotite on performance, meat quality and organ development in Ross broiler. Egyptian Poultry Science Journal 30, 77–88.

SAS Institute Incorporation (2003) ‘SAS user’s guide, version 9.1.’ (SAS Institute Incorporation: Cary, NC)

Sayama K, Lin S, Zheng G, Oguni I (2000) Effect of green tea on growth, food utilization and lipid metabolism in mice. In Vivo 14, 481–484.

Shin SH, Chae SY, Ha MH, Jo SK, Kim SH, Byun MW, Yee ST (2004) Effect of Bu-Zhong-Yi-Qi-Tang on B cell development. Journal of the Korean Society of Food Science and Nutrition 33, 271–277.
| CrossRef |

Tardani A, Terreni M (1996) Bacillus cereus in diets for pigs. Rivista di Suinicoltura 37, 27–31.

Tejada-Simon MV, Ustunol Z, Pestka JJ (1999) Ex vivo effects of Lactobacilli, Streptococci, and Bifidobacteria ingestion on cytokine and nitric oxide production in a murine model. Journal of Food Protection 62, 162–169.

Terada A, Hara H, Nakajyo S, Ichikawa H, Hara Y, Fukai K, Kobayashi Y, Mitsuoka T (1993) Effect of supplements of tea polyphenols on the cecal flora and cecal metabolites of chicks. Microbial Ecology in Health and Disease 6, 3–9.
| CrossRef |

Tough DF, Sun S, Sprent J (1997) T cell stimulation in vivo by lipopolysaccharide (LPS). The Journal of Experimental Medicine 185, 2089–2094.
| CrossRef | CAS |

Tsujii H, Kuwabara Y, Salma U, Miah AG, Nishimura M, Maki T (2008) Effect of dietary Rhodobacter capsulatus on cholesterol, triglycerides concentration, and meat quality of finishing pigs. Animal Science Journal 79, 460–465.
| CrossRef | CAS |

Turner JL, Pas , Dritz SS, Minton JE (2001) Alternatives to conventional antimicrobials in swine diets. The Professional Animal Scientist 17, 217–226.

Uuganbayar D, Shin IS, Yang CJ (2006) Comparative performance of hens fed diets containing Korean, Japanese and Chinese green tea. Asian-Australasian Journal of Animal Sciences 19, 1190–1196.

Vianna RCS, Gomes RN, Bozza FA, Amâncio RT, Bozza PT, David CMN, Castro-Faria-Neto HC (2004) Antibiotic treatment in a murine model of sepsis: impact on cytokines and endotoxin release. Shock 21, 115–120.
| CrossRef | CAS |

Witte VC, Krause GF, Bailey EM (1970) A new extraction method for determining 2-thiobarbituric acid values of pork and beef during storage. Journal of Food Science 35, 582–585.
| CrossRef | CAS |

Wu LY, Juan CC, Ho LT, Hsu YP, Hwang LS (2004) Effect of green tea supplementation on insulin sensitivity in sprague-dawley rats. Journal of Agricultural and Food Chemistry 52, 643–648.
| CrossRef | CAS |

Wu D, Guo Z, Ren Z, Guo W, Meydani SN (2009) Green tea EGCG suppresses T cell proliferation through impairment of IL-2/IL-2 receptor signaling. Free Radical Biology & Medicine 47, 636–643.
| CrossRef | CAS |

Yang CJ, Yang IY, Oh DH, Bae IH, Cho SG, Kong IG, Uuganbayar D, Nou IS, Choi KS (2003) Effect of green tea by-product on performance and body composition in broiler chicks. Asian–Australasian Journal of Animal Sciences 16, 867–872.

Yokozawa T, Dong E (1997) Influence of green tea and its three major components upon low-density lipoprotein oxidation. Experimental and Toxicologic Pathology 49, 329–335.
| CrossRef | CAS |

Zanchi R, Canzi E, Molteni L, Scozzoli M (2008) Effect of Camellia sinensis L. whole plant extract on piglet intestinal ecosystem. Annals of Microbiology 58, 147–152.
| CrossRef | CAS |

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