References

AOAC (2000) ‘Official methods of analysis.’ 17th edn. (Association of Official Analytical Chemists: Gaithersburg, MD)

ARC (1984) ‘The nutrient requirement of ruminant livestock (Supplement No. 1).’ (CAB International: Wallingford, UK)

Arroyo JM, González J (2011) Effects of the ruminal comminution rate and microbial contamination of particles on accuracy of in situ estimates of ruminal degradability and intestinal digestibility of feedstuffs. Journal of Animal Physiology and Animal Nutrition
| CrossRef |

Arroyo JM, González J, Rodríguez CA, Alvir MR, Ouarti M (2009) In situ evaluation of the protein value of wheat grain corrected for ruminal microbial contamination. Journal of the Science of Food and Agriculture 89, 731–734.
| CrossRef | CAS |

BOE (2005) Real Decreto 1201/2005 ‘About protection of animals used for experimental and other scientific purposes’. Boletín Oficial del Estado [In Spanish] 252, 34 367–34 391.

Centraal Veevoederbureau 2002 ‘Veevodertabel (Dutch Feeding Tables).’ (Centraal Veevoederbureau: Lelystad)

Crooker BA, Clark JH, Shanks RD, Fahey GC (1987) Effects of ruminal exposure on the amino acid profile of feeds. Canadian Journal of Animal Science 67, 1143–1148.
| CrossRef | CAS |

Degussa (1996) ‘The amino acid composition of feedstuffs.’ 4th edn. (Degussa AG: Frankfurt am Main, Germany)

Dhanoa MS, Siddons RC, France J, Gale DL (1985) A multicompartmental model to describe marker excretion patterns in ruminant faeces. The British Journal of Nutrition 53, 663–671.
| CrossRef | CAS |

Ellis W, Wylie MJ, Matis JH (1979) Quantitating ruminal turnover. Federation Proceedings 38, 2702–2706.

González J, Rodríguez CA, Andrés SG, Alvir MR (1998) Rumen degradability and microbial contamination of fish meal and meat meal measured by the in situ technique. Animal Feed Science and Technology 73, 71–84.
| CrossRef |

González J, Sánchez L, Alvir MR (1999) Estimation of intestinal digestibility of undegraded sunflower meal protein from nylon bag measurements. A mathematical model. Reproduction, Nutrition, Development 39, 607–616.
| CrossRef |

González J, Centeno C, Lamrani F, Rodríguez CA (2001) In situ rumen degradation of amino acids from different feeds corrected for microbial contamination. Animal Research 50, 253–264.
| CrossRef |

González J, Ouarti M, Rodríguez CA, Alvir MR (2006) Effects of considering the rate of comminution of particles and microbial contamination on the accuracy of in situ studies of feed protein degradability in ruminants. Animal Feed Science and Technology 125, 89–98.
| CrossRef |

González J, Faría-Mármol J, Rodríguez CA, Martinez A (2007) Effects of ensiling on ruminal degradability and intestinal digestibility of Italian rye-grass. Animal Feed Science and Technology 136, 38–50.
| CrossRef |

González J, Ouarti M, Rodríguez CA, Centeno C (2009) A simplified management of the in situ evaluation of feedstuffs in ruminants: application to the study of the digestive availability of protein and amino acids corrected for the ruminal microbial contamination. Archives of Animal Nutrition 63, 304–320.
| CrossRef |

González J, Faría-Marmol J, Arroyo JM, Centeno C (2010) Effects of ensiling on in situ ruminal degradability and intestinal digestibility of corn forage. Archives of Animal Nutrition 64, 204–220.
| CrossRef |

González J, Arroyo JM, Ouarti M, Guevara-González J, Rodríguez CA, Alvir MR, Moya VJ, Piquer O (2012) Composition of free and adherent ruminal bacteria: inaccuracy of the microbial nutrient supply estimates obtained using free bacteria as reference samples and 15N as marker. Animal 6, 468–475.
| CrossRef |

Liu K (1999) Chemistry and nutritional value of soybean components. In ‘Soybeans: chemistry, technology and utilization’. (Ed. Monsanto) pp. 25–114. (Aspen Publishers: Gaithersburg, MD)

Mahadevan S, Erfle JD, Sauer FD (1980) Degradation of soluble and insoluble proteins by Bacteroides amylophilus protease and by rumen microorganisms. Journal of Animal Science 50, 723–728.

McDonald P, Henderson AR, Heron SJE (1991) ‘The biochemistry of silage.’ (Chalcombe Publications: Marlow, UK)

Michalet-Doreau B, Ould-Bah MY (1989) Estimation of the extent of bacterial contamination in bag residues and its influence on in sacco measurements of forage nitrogen degradation in rumen. In ‘Proceedings of the XVI international grassland congress’. (Ed. R Jarrige) pp. 909–910. (French Grassland Society: Nice, France)

NRC (2001) ‘Nutrient requirements of dairy cattle.’ 7th revised edn. (National Academy Press: Washington, DC)

Ørskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rates of passage. The Journal of Agricultural Science 92, 499–503.
| CrossRef |

Robertson JB, Van Soest PJ (1981) The detergent system of analysis and its application to human foods. In ‘The analysis of dietary fiber in food’. (Eds WPT James, O Theander) pp. 123–142. (Marcel Dekker Inc.: New York)

Rodríguez CA, González J (2006) In situ study of the relevance of bacterial adherence to feed particles on the contamination and accuracy of rumen degradability estimates of feeds of vegetable origin. The British Journal of Nutrition 96, 316–325.
| CrossRef |

Rodríguez CA, González J, Alvir MR, Repetto JL, Centeno C, Lamrani F (2000) Composition of bacteria harvested from the liquid and solid fractions of the rumen of sheep as influenced by feed intake. The British Journal of Nutrition 84, 369–376.

SAS (1999) ‘SAS/STAT user’s guide. Version 8.’ (SAS Institute: Cary, NC)

Udén P, Colucci PE, Van Soest PJ (1980) Investigation of chromium, cerium and cobalt as markers in digesta. Rate of passage studies. Journal of the Science of Food and Agriculture 31, 625–632.
| CrossRef |

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| CrossRef | CAS |

Varvikko T (1986) Microbially corrected amino acid composition of rumen-undegraded feed protein and amino acid degradability in the rumen of feeds enclosed in nylon bags. The British Journal of Nutrition 56, 131–140.
| CrossRef | CAS |

Vérité T, Chapoutot P, Michalet-Doreau B, Peyraud JL, Poncet C (1987) ‘Révision du système des protéines digestibles dans l’intestin (PDI).’ Bulletin Technique. Centre de Recherches Zootechniques et Vétérinaires de Theix Institut National de la Recherche Agronomique 70, 19–34.