Factors affecting the number of STRO-1+ stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Article

<< Previous

Next >>

Contents Vol 52(8)

doi:10.1071/AN12012

Food, Fibre and Pharmaceuticals from Animals

	Search




	Advanced Search



	Journal Home

	About the Journal

	Editorial Board

	Contacts



	Content

	Online Early

	Current Issue

	Just Accepted

	All Issues

	Special Issues

	Research Fronts

	Reviews

	Sample Issue



	For Authors

	General Information

	Notes for Authors

	Submit Article

	Open Access



	For Referees

	Referee Guidelines

	Review Article



	For Subscribers

	Subscription Prices

	Customer Service

	Print Publication Dates

New Feature

New Commenting Tool
Join the conversation and leave comments on all new journal articles.

e-Alerts

Subscribe to our Email Alert or

feeds for the latest journal papers.

Connect with us

Factors affecting the number of STRO-1⁺ stem cells derived from regenerating antler and pedicle cells of red and fallow deer

Erika Kužmová ^A ^B ^E, Radim Kotrba ^A, Hans J. Rolf ^C, Luděk Bartoš ^A, K. Günter Wiese ^C, Jutta Schulz ^C and George A. Bubenik ^D

^A Department of Ethology, Institute of Animal Science, Přátelství 815, 104 01 Prague – Uhříněves, Czech Republic.
^B Department of Ecology, Charles University in Prague, Albertov 6, 128 43 Prague 2, Czech Republic.
^C Department of Maxillofacial Surgery, Clinical Research, University of Göttingen, University Hospital, Robert-Koch-Str. 40, 37075 Göttingen, Germany.
^D Department of Integrative Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
^E Corresponding author. Email: kuzmovae@gmail.com

Animal Production Science 52(8) 746-749 http://dx.doi.org/10.1071/AN12012
Submitted: 13 January 2012 Accepted: 30 March 2012 Published: 14 June 2012





	PDF (265 KB) $25



	Export Citation

Print

Abstract

Mesenchymal stem cells positive to surface antigen STRO-1 were isolated from regenerating antlers of red deer (Cervus elaphus) and fallow deer (Dama dama) using a magnetic-activated cell sorting (MACS) method. In this study we analysed factors potentially affecting the number of STRO-1⁺ cells in the cell cultures. With regard to the STRO-1 antigen, we evaluated data from 188 MACS separation procedures of cell cultures cultivated in Dulbecco’s Modified Eagle Medium and 10% fetal calf serum of four fallow deer males (130 procedures) and four red deer males (58 procedures). The analysed factors were the sampling site of the antler or the pedicle, cell passage and type of the cell culture (mixed or STRO-1 negative cell cultures). The percentage of obtained STRO-1⁺ cells varied greatly from 0.4 to 38.9% for fallow deer and from 1.8 to 16.5% for red deer. We have not found any significant influence of the sampling site. The passage and the type of culture were significant factors for both fallow and red deer cells. The highest numbers of STRO-1⁺ cells were obtained from the second passage from both fallow and red deer cell cultures (24.6 and 5.5%, respectively). Our experiment revealed that we can maximise the number of STRO-1⁺ cells in the cultures by manipulating the cultivation factors.

References

Barbero A, Ploegert S, Heberer M, Martin I (2003) Plasticity of clonal populations of dedifferentiated adult human articular chondrocytes. Arthritis and Rheumatism 48, 1315–1325.
| CrossRef | CAS |

Bartoš L, Schams D, Bubenik GA (2009) Testosterone, but not IGF-1, LH, prolactin or cortisol, may serve as antler-stimulating hormone in red deer stags (Cervus elaphus). Bone 44, 691–698.
| CrossRef |

Bartoš L, Bubenik GA, Kužmorá E (2012) Endocrine relationships between rank-related behaviour and growth in deer. Frontiers in Bioscience 4, 1111–1126.

Berg DK, Li C, Asher G, Wells DN, Oback B (2007) Red deer cloned from antler stem cells and their differentiated progeny. Biology of Reproduction 77, 384–394.
| CAS |

Cegielski M, Dziewiszek W, Zabel M, Dziegiel P, Izycki D, Zatonski M, Bochnia M (2008) Experimental application of xenogenous antlerogenic cells in replacement of auricular cartilage in rabbits. Xenotransplantation 15, 374–383.
| CrossRef |

Cegielski M, Dziewiszek W, Zabel M, Dziegiel P, Kuryszko J, Izykowska I, Zatonski M, Bochnia M (2010) Experimental xenoimplantation of antlerogenic cells into mandibular bone lesions in rabbits: two-year follow-up. In Vivo (Athens, Greece) 24, 165–172.

Dennis JE, Carbillet J, Caplan AI, Charbord P (2002) The STRO-1⁺ marrow cell population is multipotential. Cells, Tissues, Organs 170, 73–82.
| CrossRef |

Kierdorf U, Kierdorf H, Szuwart T (2007) Deer antler regeneration: cells, concepts, and controversies. Journal of Morphology 268, 726–738.
| CrossRef |

Kierdorf U, Li C, Price JS (2009) Improbable appendages: deer antler renewal as a unique case of mammalian regeneration. Seminars in Cell & Developmental Biology 20, 535–542.
| CrossRef |

Kužmová E, Bartoš L, Kotrba R, Bubenik GA (2011) Effect of different factors on proliferation of antler cells, cultured in vitro. PLoS ONEs 6, e18053
| CrossRef |

Li C, Suttie JM (2001) Deer antlerogenic periosteum: a piece of postnatally retained embryonic tissue? Anatomy and Embryology 204, 375–388.
| CrossRef | CAS |

Li C, Suttie JM (2006) Recent progress in antler regeneration and stem cell research. In ‘Proceedings of the 6th international deer biology congress – advances in deer biology’. (Eds L Bartoš, A Dušek, R Kotrba, J Bartošová-Víchová) p. 18. (Research Institute of Animal Production: Prague)

Li C, Mackintosh CG, Martin SK, Clark DE (2007) Identification of key tissue type for antler regeneration through pedicle periosteum deletion. Cell and Tissue Research 328, 65–75.
| CrossRef |

Matich J, Basford Nicholson L, Barling P (2003) Mitotic activity in the growing red deer antler. Cell Biology International 27, 625–632.
| CrossRef |

Mount JG, Muzylak M, Allen S, Okushima S, Althnaian IM, Mc Gonnell IM, Price JS (2006) Antlers may regenerate from persistent neural crestlike stem cells. In ‘Proceedings of the 6th international deer biology congress – advances in deer biology’. (Eds L Bartoš, A Dušek, R Kotrba, J Bartošová-Víchová) p. 161. (Research Institute of Animal Production: Prague)

Rolf HJ, Kierdorf U, Kierdorf H, Seymour N, Napp J, Schliephake H, Wiese KG (2006) Visualisation and characterisation of stem cells from the regenerating deer antler. In ‘Proceedings of the 6th international deer biology congress – advances in deer biology’. (Eds L Bartoš, A Dušek, R Kotrba, J Bartošová-Víchová) p. 160. (Research Institute of Animal Production: Prague)

Rolf HJ, Kierdorf U, Kierdorf H, Schulz J, Seymour N, Schliephake H, Napp J, Niebert S, Wölfel H, Wiese KG (2008) Localization and characterization of STRO-1⁺ cells in the deer pedicle and regenerating antler. PLoS ONE 3, e2064
| CrossRef |

Rolf HJ, Niebert S, Niebert M, Gaus L, Schliephake H, Niese KG (2012) Intercellular transport of Oct4 in mammalian cells: a basic principle to extend a stem cell niche? PLoS ONE 7, e32287
| CrossRef | CAS |

Simmons PJ, Torok-Storb B (1991) Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood 78, 55–62.
| CAS |

Stewart K, Walsh S, Screen J, Jefferiss CM, Chainey J, Jordan GR, Beresford JN (1999) Further characterisation of cells expressing STRO-1 in cultures of adult human bone marrow stromal cells. Journal of Bone and Mineral Research 14, 1345–1356.
| CrossRef | CAS |

Yu J, He H, Tang Ch, Zhang G, Li Y, Wang R, Shi J, Jin Y (2010) Differentiation potential of STRO-1⁺ dental pulp stem cells changes during cell passaging. BMC Cell Biology 11,
| CrossRef |

Subscriber Login

	Username: Password:

Legal & Privacy | Contact Us | Help