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Protocols in ecological and environmental plant physiology

 

Article << Previous     |         Contents Vol 57(4)

Propagation and reintroduction of Caladenia

Magali Wright A B H, Rob Cross B, Kingsley Dixon C, Tien Huynh D, Ann Lawrie D, Les Nesbitt E, Andrew Pritchard F, Nigel Swarts C, Richard Thomson B G

A Melbourne School of Land and Environment, Burnley Campus, The University of Melbourne, 500 Yarra Blvd, Richmond, Vic. 3121, Australia.
B Royal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Vic. 3141, Australia.
C Kings Park and Botanic Garden, Fraser Avenue, West Perth, WA 6005, Australia.
D Biotechnology & Environmental Biology, School of Applied Sciences, RMIT University, Bundoora West Campus, Bundoora, Vic. 3083, Australia.
E PO BOX 72, Walkerville, SA 5081, Australia.
F Department of Sustainability and Environment, 78 Henna Street, Warrnambool, Vic. 3280, Australia.
G Australasian Native Orchid Society (Victorian Group), PO Box 354, Glen Waverley, Vic. 3150, Australia.
H Corresponding author. Email: mmwright@unimelb.edu.au
 
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Abstract

Many Caladenia species have been reduced to extremely small and/or fragmented populations, and reintroduction/translocation into natural or rehabilitated habitats, by using ex situ propagated plants or via direct seeding, represents an important adjunct in conservation planning. However, Caladenia species are some of the most difficult terrestrial orchid taxa to propagate, in part because of the specificity of the mycorrhizal associations and the need to provide growing conditions that suit both the mycorrhizal fungi and Caladenia plants. The present paper reviews recent advances in Caladenia propagation and reintroduction methods, including in vitro seed germination, transferral from in vitro to nursery environments, ex vitro symbiotic germination (germination in inoculated nursery media), nursery cultivation, the use of nurse plants and reintroduction of Caladenia into natural habitats by using seed, dormant tubers or growing plants. Techniques discussed in the present paper increase the options for future Caladenia conservation programs, especially for those species currently on the brink of extinction.

   
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