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Protocols in ecological and environmental plant physiology

 

Article     |     Next >>   Contents Vol 59(6)

Protein content and electrophoretic profile of insect galls on susceptible and resistant host plants of Bauhinia brevipes Vogel (Fabaceae)

Michelle de Lima Detoni A F, Eveline Gomes Vasconcelos A, Ana Carolina Ribeiro Gomes Maia A, Michélia Antônia do Nascimento Gusmão A, Rosy Mary dos Santos Isaias B, Geraldo Luiz Gonçalves Soares C, Jean Carlos Santos D and G. Wilson Fernandes E

A Laboratório de Estudos de Estrutura e Função de Proteínas, Departamento de Bioquímica, ICB, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
B Departamento de Botânica, ICB, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
C Departamento de Botânica, IB, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
D Instituto de Biologia, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
E Laboratório de Ecologia Evolutiva e Biodiversidade/DBG, ICB, Universidade Federal de Minas Gerais, CP 486, Belo Horizonte, MG 30161 901, Brazil.
F Corresponding author. Email: michelledetoni@yahoo.com.br

Australian Journal of Botany 59(6) 509-514 http://dx.doi.org/10.1071/BT11104
Submitted: 8 April 2011  Accepted: 25 July 2011   Published: 5 October 2011


 
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Abstract

Gall induction, mediated by insect-herbivore chemical stimuli, is the result from anatomical and biochemical alterations in the host-plant tissues that provides shelter, food and defence against natural enemies and the harsh environment to the gall inducer. Schizomya macrocapillata Maia (Diptera, Cecidomyiidae) induces galls on Bauhinia brevipes Vogel (Fabaceae); the galls are spherical, with long reddish hairs that cover the adaxial wall surface of the gall, and a protuberance is observed on the abaxial leaf surface. Some plants are resistant to gall formation and, in many cases, this formation is inhibited by hypersensitive reaction. In the present work, samples from different parts of the non-galled and galled tissues from resistant and susceptible plants were carefully dissected. Indicating elevated metabolic activity, the protein concentration was 1.5–4.5-fold higher in the abaxial portion of the galls than in any other tissues, regardless of whether the galls were from resistant or susceptible plants. Different tissues from susceptible and resistant plants had distinct protein concentrations, and the fractionation of the proteins by SDS–PAGE and silver-staining showed shared and/or specific polypeptides. We hypothesise that specific proteins, possibly from distinct metabolic pathways, are involved in the physiological processes that determine whether the plant shows total and/or partial host resistance to the galling-insect attack.



References

Allison SD, Schultz JC (2005) Biochemical responses of chestnut oak to a galling cynipid. Journal of Chemical Ecology 31, 151–166.
CrossRef | CAS |

Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
CrossRef | CAS |

Cornelissen TG, Fernandes GW (1998) Plant hypersensitivity against tissue invasive insects: Bauhinia brevipes and Contarinia sp. interaction. In ‘The biology of gall inducing arthropods’. (Eds G Csóka, WJ Mattson, GN StoneN, PW Price) pp. 144–152. (US Departament of Agriculture, Forest Service: Saint Paul, MN)

Cornelissen TG, Fernandes GW (2001) Patterns of attack by herbivores on the tropical shrub Bauhinia brevipes (Leguminosae): vigor or chance? European Journal of Entomology 98, 37–40.

Cornelissen TG, Negreiros D, Fernandes GW (2002) Plant resistance against gall-forming insects: the role of hypersensitivity. In ‘Mechanisms and deployment of resistance in trees to insects’. (Eds MR Wagner, KM Clancy, F Lieutier) pp. 137–152. (Kluwer Academic Publishers: Dordrecht, Netherlands)

Cornell HV (1983) The secondary chemistry and complex morphology of galls formed by the Cynipinae (Hymenoptera): why and how? American Midland Naturalist 110, 225–234.
CrossRef |

Detoni ML, Vasconcelos EG, Scio E, Aguiar JA, Isaias RMS, Soares GLG (2010) Differential biochemical responses of Calliandra brevipes (Fabaceae, Mimosoidae) to galling behaviour by Tanaostigmodes ringueleti and T. mecanga (Hymenoptera, Tanaostigmatidae). Australian Journal of Botany 58, 280–285.
CrossRef | CAS |

Fernandes GW (1990) Hypersensitivity: a neglected plant resistance mechanism against insect herbivores. Environmental Entomology 19, 1173–1182.

Fernandes GW (1998) Hypersensitivity as a phenotypic basis of plant induced resistance against a galling insect (Diptera: Cecidomyiidae). Environmental Entomology 27, 260–267.

Fernandes GW, Negreiros D (2001) The occurrence and effectiveness of hypersensitive reaction against galling herbivores across host taxa. Ecological Entomology 26, 46–55.
CrossRef |

Fernandes GW, Price PW (1988) Biogeographical gradients in galling species richness. Tests of hypotheses. Oecologia 76, 161–167.
CrossRef |

Fernandes GW, Cornelissen TG, Isaias RMS, Lara TAF (2000) Plants fight gall formation: hypersensitivity. Ciencia e Cultura 52, 49–54.

Harris MO, Stuart JJ, Mohan M, Nair S, Lamb RJ, Rohfritsch O (2003) Grasses and gall midges: plant defense and insect adaptation. Annual Review of Entomology 48, 549–577.
CrossRef | CAS |

Hartley SE (1998) The chemical composition of plant galls: are levels of nutrients and secondary compounds controlled by the gall-former? Oecologia 113, 492–501.
CrossRef |

Hilker H, Rohfritsch O, Meiners T (2002). The plant’s response towards insect egg deposition. In ‘Chemoecology of insect eggs and egg deposition’. (Eds M Hilker, T Meiners) pp. 205–233. (Blackwell Publishing: Berlin)

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophages T4. Nature 227, 680–685.
CrossRef | CAS |

Maia VC, Fernandes GW (2005) Two new species of Asphondyliini (Diptera: Cecidomyiidae) associated with Bauhinia brevipes (Fabaceae) in Brazil. Zootaxa 1091, 27–40.

Price PW, Fernandes GW, Warring GL (1987) Adaptative nature of insect galls. Environmental Entomology 16, 15–24.

Raman A, Schaefer CW, Withers TM (2005) ‘Biology, ecology, and evolution of gall-inducing arthropods.’ (Science Publishers Inc.: Enfield, NH)

Rohfritsch O (2008) Plants, gall midges, and fungi: a three-component system. Entomologia Experimentalis et Applicata 128, 208–216.
CrossRef |

Sá CEM, Silveira FAO, Santos JC, Isaias RMS, Fernandes GW (2009) Anatomical and developmental aspects of leaf galls induced by Schizomya macropillata Maia (Diptera: Cecidomyiidae) on Bauhinia brevipes Vogel (Fabaceae). Revista Brasileira de Botânica 32, 319–327.
CrossRef |

Santos JC, Silveira FAO, Fernandes GW (2008) Long term oviposition preference and larval performance of Schizomyia macrocapillata (Diptera: Cecidomyiidae) on larger shoots of its host plant Bauhinia brevipes (Fabaceae). Evolutionary Ecology 22, 123–137.
CrossRef |

Schönrogge K, Harper LJ, Lichtenstein CP (2000) The protein content of tissues in cynipid galls (Hymenoptera: Cynipidae): similarities between cynipid galls and seeds. Plant, Cell & Environment 23, 215–222.
CrossRef |


   
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