CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Australian Journal of Botany   
Australian Journal of Botany
Journal Banner
  Southern Hemisphere Botanical Ecosystems
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Turner Review Series
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook   youtube

red arrow PrometheusWiki
blank image
PrometheusWiki
Protocols in ecological and environmental plant physiology

 

Article << Previous     |     Next >>   Contents Vol 60(4)

Structure, function and secretory products of the peltate glands of Centrolobium tomentosum (Fabaceae, Faboideae)

Esmeire Cruz Matos A and Élder Antônio Sousa Paiva B C

A Fundação Ezequiel Dias – FUNED, Laboratório de Microscopia de Produtos, 30510-010, Belo Horizonte, MG, Brazil.
B Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901, Belo Horizonte, MG, Brazil.
C Corresponding author. Email: epaiva@icb.ufmg.br

Australian Journal of Botany 60(4) 301-309 http://dx.doi.org/10.1071/BT12009
Submitted: 16 January 2012  Accepted: 13 April 2012   Published: 19 June 2012


 
PDF (1.2 MB) $25
 Export Citation
 Print
  
Abstract

The glandular structures of Centrolobium tomentosum Guill. ex Benth. have been little studied despite the economic importance of this species. We describe here the distribution, development, structure and ultrastructure of the secretory cells of the peltate glands found on the vegetative organs of this species. Stem apices and leaves in various stages of development were collected and prepared for examination by light, scanning and transmission electron microscopy. Chemical analyses and conventional histochemical tests to determine the chemical nature of the secretory products were also carried out. Peltate glands occur on aerial vegetative organs during their primary growth stage. These trichomes are structurally stable, persisting throughout the development of the organ. During the initial stages of the gland development, cell separation creates a central space that expands as secretions accumulate. Maximum secretion rates occur during this phase and the secreting cells characteristically have well developed smooth and rough endoplasmic reticulum, and high numbers of plastids and mitochondria. During the later stages of the secretory phase, the central cells show symptoms of cell death and are incorporated in to the secretions. At trichome maturity, the central space is delimited by a uniseriate epithelium. In addition to the resin, which is the main secretory product, an extensive three-dimensional carbohydrate matrix was observed that extended throughout the central space, apparently giving support to the resin droplets. The terpenic nature of the secretion was confirmed by thin-layer chromatography. Given the terpenic nature of the secretion and the permanence of trichomes throughout all phases of leaf development, it is postulated that the resin-secreting trichomes act to protect the plant against herbivores.



References

Argyropoulou C, Akoumianaki-Ioannidou A, Christodoulakis NS, Fasseas C (2010) Leaf anatomy and histochemistry of Lippia citriodora (Verbenaceae). Australian Journal of Botany 58, 398–409.
CrossRef |

Bell JM, Curtis J (1985) Development and ultrastructure of foliar glands of Comptonia peregrina (Myricaceae). Botanical Gazette 146, 288–292.
CrossRef |

Bennici A, Tani C (2004) Anatomical and ultrastructural study of the secretory cavity development of Citrus sinensis and Citrus limon: evaluation of schizolysigenous ontogeny. Flora 199, 464–475.
CrossRef |

Bisio A, Corallo A, Gastaldo P, Romussi G, Ciarallo G, Fontana N, De Tommasi N, Profumo P (1999) Glandular hairs and secreted material in Salvia blepharophylla Brandegee ex Epling grown in Italy. Annals of Botany 83, 441–452.
CrossRef | CAS |

Bosabalidis AM (1990) Glandular trichomes in Satureja thymbra leaves. Annals of Botany 65, 71–78.

Bosabalidis AM (2010) Ultrastructure, development and histochemistry of the polysaccharide-containing subcuticular compartments in Origanum dictamus L. peltate glandular hairs. Flavour and Fragrance Journal 25, 202–205.
CrossRef | CAS |

Brundrett MC, Kendrick B, Peterson CA (1991) Efficient lipid staining in plant material with Sudan Red 7B or fluoral yellow 088 in polyethylene glycol–glycerol. Biotechnic & Histochemistry 66, 111–116.
CrossRef | CAS |

Choi JS, Lee NY, Oh SE, Son KC, Kim ES (2011) Developmental ultrastructure of glandular trichomes of Rosmarinus officinalis: secretory cavity and secretory vesicle formation. Journal of Plant Biology 54, 135–142.
CrossRef |

David R, Carde JP (1964) Colaration différentielle des inclusions lipidique et terpeniques des pseudophylles du pine maritime au moyen du reactif Nadi. Comptés Rendus de L’Academie des Sciences Paris, Série D 258, 1338–1340.

Dell B, McComb AJ (1979) Plant resins: their formation, secretion and possible functions. Advances in Botanical Research 6, 277–316.
CrossRef |

Durkee LT, Baird CW, Cohen PF (1984) Light and electron microscopy of resin glands of Passiflora foetida (Passifloraceae). American Journal of Botany 71, 596–602.
CrossRef |

Fahn A (2002) Functions and location of secretory tissues in plants and their possible evolutionary trends. Israel Journal of Plant Sciences 50, S59–S64.
CrossRef |

Feucht W, Schmid PPS, Christ E (1986) Distribution of flavonols in meristematic tissues of Prunus avium shoots. Journal of Plant Physiology 125, 1–8.
CrossRef | CAS |

Gaffal KP, Friedrichis GJ, El-Gammal S (2007) Ultrastructural evidence for a dual function of the phloem and programmed cell death in the floral nectary of Digitalis purpurea. Annals of Botany 99, 593–607.
CrossRef |

Giuliani C, Bini LM (2008) Insight into the structure and chemistry of glandular trichomes of Labiatae, with emphasis on subfamily Lamioideae. Plant Systematics and Evolution 276, 199–208.
CrossRef |

Heil M, McKey D (2003) Protective ant–plant interactions as model systems in ecological and evolutionary research. Annual Review of Ecology Evolution and Systematics 34, 425–453.
CrossRef |

Jensen WA (1962) ‘Botanical histochemistry: principles and practice.’ (W.H. Freeman: San Francisco)

Johansen DA (1940) ‘Plant microtechnique.’ (McGraw Book: New York)

Karnovsky MJ (1965) A formaldehyde–glutaraldehyde fixative of light osmolality for use in eletron microscopy. The Journal of Cell Biology 27, 137A–138A.

Kennedy GG (2003) Tomato, pest, parasitoids, and predators: tritrophic interactions involving the genus Lycopersicon. Annual Review of Entomology 48, 51–72.
CrossRef | CAS |

Langenheim JH (2003) ‘Plant resins: chemistry, evolution, ecology and ethnobotany.’ (Timber Press: Cambridge, UK)

Lapinjoki SP, Elo HA, Taipali HT (1991) Development and structure of resin glands on tissues of Betula pendula Roth, during growth. New Phytologist 117, 219–223.
CrossRef |

Lersten NR, Curtis JD (1996) Survey of leaf anatomy, especially secretory structures, of tribe Caesalpinieae (Leguminosae, Caesalpinioideae). Plant Systematics and Evolution 200, 21–39.
CrossRef |

Lima HC (1985) Centrolobium Martius ex Bentham (Leguminosae-Papilionoideae) estudo taxonômico das espécies brasileiras extra-amazônicas. Arquivos do Jardim Botânico do Rio de Janeiro XXVII, 177–191.

Machado SR, Gregório EA, Guimarães E (2006) Ovary peltate trichomes of Zeyheria montana (Bignoniaceae): developmental ultrastructure and secretion in relation to function. Annals of Botany 97, 357–369.
CrossRef |

O’Brien TP, Feder N, McCully ME (1964) Polychromatic staining of plant cell walls by toluidine blue. Protoplasma 59, 368–373.
CrossRef | CAS |

O’Brien SP, Loveys BR, Grant WJR (1996) Ultrastructure and function of floral nectaries of Chamelaucium uncinatum (Myrtaceae). Annals of Botany 78, 189–196.
CrossRef |

Onelli E, Rivetta A, Giorgi A, Bignami M, Cocucci M, Patrignani G (2002) Ultrastructural studies on the developing secretory nodules of Hypericum perforatum. Flora 197, 92–102.
CrossRef |

Paiva EAS (2009) Ultrastructure and post-floral secretion of the pericarpial nectaries of Erythrina speciosa (Fabaceae). Annals of Botany 104, 937–944.
CrossRef |

Paiva EAS, Machado SR (2006) Structural and ultrastructural aspects of ontogenesis and differentiation of resin secretory cavities in Hymenaea stigonocarpa (Fabaceae–Caesalpinioideae) leaves. Nordic Journal of Botany 24, 423–431.
CrossRef |

Paiva EAS, Martins LC (2011) Calycinal trichomes in Ipomoea cairica (Convolvulaceae): ontogenesis, structure and functional aspects. Australian Journal of Botany 59, 91–98.
CrossRef | CAS |

Pirie MD, Klitgaard BB, Pennington RT (2009) Revision and Biogeography of Centrolobium (Leguminosae–Papilionoideae). Systematic Botany 34, 345–359.
CrossRef |

Ranger CM, Winter REK, Rottinghaus GE, Backus EA, Johnson DW (2005) Mass spectral characterization of fatty acid amides from alfalfa trichomes and their deterrence against the potato leafhopper. Phytochemistry 66, 529–541.
CrossRef | CAS |

Robards AW (1978) An introduction to techniques for scanning electron microscopy of plant cells. In ‘Electron microscopy and cytochemistry of plant cells’. (Ed. JL Hall) pp. 343–403. (Elsevier: New York)

Rodrigues TM, Teixeira SP, Machado SR (2011) The oleoresin secretory system in seedlings and adult plants of copaíba (Copaifera langsdorffii Desf., Leguminosae–Caesalpinioideae). Flora 206, 585–594.
CrossRef |

Roland AM (1978) General preparations and staining of thin sections. In ‘Electron microscopy and cytochemistry of plant cells’. (Ed. JL Hall) pp. 1–62. (Elsevier: New York)

Russell GB (1977) Plant chemicals affecting insect development. New Zealand Entomologist 6, 229–234.

Shorey HH, Gaston LK, Gerber RG, Sisk CB, Phillips PA (1996) Formulating farnesol and other ant-repellent semiochemicals for exclusion of Argentine ants (Hymenoptera: Formicidae) from citrus trees. Environmental Entomology 25, 114–119.

Solereder H (1908) ‘Systematic anatomy of the dicotyledons. Vol. I.’ (Clarendon Press: Oxford, UK)

Tang XR, Chen SL, Wang L (2011) Isolation and Insecticidal activity of farnesol from Stellera chamaejasme. Asian Journal of Chemistry 23, 1233–1235.

Turner GW (1986) Comparative development of secretory cavities in the tribes Amorpheae and Psoraleeae (Leguminosae: Papilionoideae). American Journal of Botany 73, 1178–1192.
CrossRef |

Turner GW, Croteau R (2004) Organization of monoterpene biosynthesis in Mentha. Immunocytochemical localizations of geranyl diphosphate synthase, limonene-6-hydroxylase, isopiperitenol dehydrogenase, and pulegone reductase. Plant Physiology 136, 4215–4227.
CrossRef | CAS |

Wagner H, Bladt S (2001) ‘Plant drug analysis: a thin layer chromatography atlas.’ (Springer-Verlag: Berlin)

Wagner GJ, Wang E, Shepherd RW (2004) New approaches for studying and exploiting an old protuberance, the plant trichome. Annals of Botany 93, 3–11.
CrossRef | CAS |

Werker E (2000) Trichome diversity and development. Advances in Botanical Research 31, 1–35.
CrossRef |


   
Subscriber Login
Username:
Password:  

 
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2013