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Protocols in ecological and environmental plant physiology

 

Article << Previous     |     Next >>   Contents Vol 60(7)

Marked differences in genetic diversity and differentiation between the centre and edge of the geographical range of Megaleranthis saniculifolia (Ranunculaceae), a Korean endemic species

Ji Hee Jeong A and Zin-Suh Kim A B

A Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-713, South Korea.
B Corresponding author. Email: zskim@korea.ac.kr

Australian Journal of Botany 60(7) 582-591 http://dx.doi.org/10.1071/BT12033
Submitted: 10 February 2012  Accepted: 28 July 2012   Published: 12 September 2012


 
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Abstract

The amount and distribution of genetic diversity within and between Megaleranthis saniculifolia Ohwi populations were compared between the central and peripheral regions of the species distribution. Allozyme and ISSR markers were used for genetic analysis of six populations from the central region (DY) and five populations from the peripheral region (MJ). Genetic diversity was substantially higher in the DY region than in the MJ region. Relatively uniform homozygote excess at many loci in most populations indicated that M. saniculifolia was influenced by a substantial degree of inbreeding in both regions. The degree of differentiation between populations was remarkably higher in the MJ region than in the DY region. Cluster analysis showed a trend towards separation between regions, although populations in the MJ region exhibited a slightly different trend according to the markers. We conclude that genetic drift has been affecting the populations in the MJ region for a long time, on the basis of their low genetic diversity, high differentiation, U-shaped allele-frequency distribution, and fixation of alleles towards opposing frequencies (1 or 0) among populations. In contrast, the DY region maintained relatively stable populations, although evidence of a recent bottleneck was found in one population. Along with some practical measures for genetic conservation, we present an optimal sample size for ex situ conservation to secure as many common alleles as possible.



References

Agrimonti G, Bianchi R, Bianchi A, Ballero M, Poil F, Marmiroli N (2007) Understanding biological conservation strategies: a molecular-genetic approach to the case of myrtle (Myrtus communis L.) in two Italian regions: Sardinia and Calabria. Conservation Genetics 8, 385–396.
CrossRef | CAS |

Allendorf FW (1986) Genetic drift and the loss of alleles versus heterozygosity. Zoo Biology 5, 181–190.
CrossRef |

Allendorf FW, Luikart G (2006) ‘Conservation and the genetics of populations.’ (Blackwell: Malden, MA)

Black WC, IV (1997) ‘BIOSYS-2. A computer program for the analysis of allelic variation in genetics’ (Colorado State University: Fort Collins, CO)

Black WC, IV (1998) ‘FORTRAN programs for the analysis of RAPD–PCR markers in populations.’ (Colorado State University: Fort Collins, CO)

Buza L, Young A, Thrall P (2000) Genetic erosion, inbreeding and reduced fitness in fragmented populations of the endangered tetraploid pea Swainsona recta. Biological Conservation 93, 177–186.
CrossRef |

Chang C-S, Kim H, Kim Y-S (2001) Reconsideration of rare and endangered plant species in Korea based on the IUCN Red List categories. Korean Journal of Plant Taxonomy [in Korean] 31, 107–142.

Chang C-S, Choi DY, Kim H, Park TY, Kim Y-S (2005) Patterns of allozymes variation in relation to population size of the threatened plant Megaleranthis saniculifolia (Ranunculaceae) in Korea. Journal of Plant Biology 48, 339–350.
CrossRef | CAS |

Chung MY (2009) Low levels of genetic variation within populations of the four rare orchids Gymnadenia cucullata, Gymnadenia camtschatica, Amitostigma gracile, and Pogonia minor in South Korea: indication of genetic drift and implications for conservation. Plant Systematics and Evolution 281, 65–76.
CrossRef |

Chung MY, Nason JD, Chung MG (2005) Spatial genetic structure in populations of the terrestrial orchid Orchis cyclochila (Orchidaceae). Plant Systematics and Evolution 254, 209–219.
CrossRef |

Conkle M, Hodgsksss P, Nunnally L, Hunter S (1982) Starch gel electrophoresis of conifer seeds: a laboratory manual. General Technical Report PSW-64. Pacific Southwest Forest and Range Experiment Station, Forest Service, United States Department of Agriculture, Berkeley, CA, USA.

Crnokrak P, Roff DA (1999) Inbreeding depression in the wild. Heredity 83, 260–270.
CrossRef |

Eckstein RL, O’Neill RA, Danihelka J, Otte A, Köhler W (2006) Genetic structure among and within peripheral and central populations of three endangered floodplain violets. Molecular Ecology 15, 2367–2379.
CrossRef | CAS |

Ellstrand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annual Review of Ecology and Systematics 24, 217–242.
CrossRef |

Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 47–50.

Felsenstein J (1993) ‘PHYLIP v3.5c.’ (University of Washington: Seattle, WA)

Frankham R, Ballou JD, Briscoe DA (2002) ‘Introduction to conservation genetics.’ (Cambridge University Press: Cambridge)

Godt MJW, Hamrick JL, Bratton S (1995) Genetic diversity in a threatened wetland species, Helonias bullata (Liliaceae). Conservation Biology 9, 596–604.
CrossRef |

Goudet J (2002) ‘FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3.2).’ Available at http://www.unil.ch/izea/softwares/fstat.html [accessed May 2010].

Hartl DL, Clark AG (2007) ‘Principles of population genetics.’ (Sinauer Associates: Sunderland, MA)

Hilfiker K, Gugerli F, Schütz J-P, Rotach P, Holderegger R (2004) Low RAPD variation and female-biased sex ratio indicate genetic drift in small populations of the dioecious conifer Taxus baccata in Switzerland. Conservation Genetics 5, 357–365.
CrossRef | CAS |

Honnay O, Jacquemyn H (2007) Susceptibility of common and rare plant species to the genetic consequences of habitat fragmentation. Conservation Biology 21, 823–831.
CrossRef |

Jang SK, Cheon KS, Jeong JH, Kim ZS, Yoo KO (2009) Environmental characteristics and vegetation of Megaleranthis saniculifolia habitats. Korean Journal of Environmental Biology [in Korean] 27, 314–322.

Jeong JH, Kim EH, Guo W, Yoo KO, Jo DG, Kim ZS (2010) Genetic diversity and structure of the endangered species Megaleranthis saniculifolia in Korea as revealed by allozyme and ISSR markers. Plant Systematics and Evolution 289, 67–76.
CrossRef | CAS |

Kim Z-S, Yi C-H, Lee S-W (1994) Genetic variation and sampling strategy for conservation in Pinus species. In ‘Conservation and manipulation of genetic resources in forestry’. (Eds Z-S Kim, HH Hattemer) pp. 294–321. (Kwang Moon Kag Publishing Co.: Seoul)

Kim Z-S, Jeong J-H, Wang R, Mao Z (2010) Optimal sampling strategies for conservation of Pinus densiflora based on genetic variation parameters in Korea and China. International Forestry Review 12, 101

Korea National Arboretum (2009) ‘Rare plants data book of Korea.’ (GeoBook Publishing: Pocheon, Gyeonggi-Do, Korea)

Lammi A, Siikamäki P, Mustajärvi K (1999) Genetic diversity, population size and fitness in central and peripheral populations of a rare plant Lychnis viscaria. Conservation Biology 13, 1069–1078.
CrossRef |

Leimu R, Mutikainen P, Koricheva J, Fischer M (2006) How general are positive relationships between plant population size, fitness and genetic variation? Journal of Ecology 94, 942–952.
CrossRef |

Lesica P, Allendorf FW (1995) When are peripheral populations valuable for conservation? Conservation Biology 9, 753–760.
CrossRef |

Luijten SH, Dierick A, Gerard J, Oostermeijer B, Raijmann LEL, Den Nijs HCM (2000) Population size, genetic variation and reproductive success in rapidly declining self-incompatible perennial (Arnica montana) in the Netherlands. Conservation Biology 14, 1776–1787.
CrossRef |

Marshall DR, Brown AHD (1975) Optimum sampling strategies in genetic conservation. In ‘Crop genetic resources for today and tomorrow’. (Eds OH Frankel, JG Hawkes) pp. 53–80. (Cambridge University Press: Cambridge, UK)

Neel MC, Cummings MP (2003) Effectiveness of conservation targets in capturing genetic diversity. Conservation Biology 17, 219–229.
CrossRef |

Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, USA 70, 3321–3323.
CrossRef | CAS |

Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89, 583–590.

Nei M, Maruyama T, Chakraborty R (1975) The bottleneck effect and genetic variability in populations. Evolution 29, 1–10.
CrossRef |

Peakall R, Smouse PE (2006) GENEALEX 6: genetic analysis in Excel population genetic software for teaching and research. Molecular Ecology Notes 6, 288–295.
CrossRef |

Piry S, Luikart G, Cornuet JM (1999) BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. The Journal of Heredity 90, 502–503.
CrossRef |

Rether B, Delmas G, Laouedj A (1993) Isolation of polysaccharidefree DNA from plants. Plant Molecular Biology Reporter 11, 333–337.
CrossRef | CAS |

Shannon CE, Weaver W (1949) ‘The mathematical theory of communication.’ (University of Illinois Press: Urbana, IL)

Swofford DL, Selander RB (1981) BIOSYS-1: FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematic. Journal of Heredity 72, 281–283.

Van Rossum F, Triest L (2006) Fine-scale genetic structure of the common Primula elatior (Primulaceae) at an early stage of population fragmentation. American Journal of Botany 93, 1281–1288.
CrossRef |

Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38, 1358–1370.
CrossRef |

Wolf AT, Harrison SP, Hamrick JL (2000) Influence of habitat patchiness on genetic diversity and spatial structure of a serpentine endemic plant. Conservation Biology 14, 454–463.
CrossRef |

Wright S (1949) The genetical structure of populations. Annals of Eugenics 15, 323–354.
CrossRef |

Wright S (1977) ‘Evolution and the genetics of populations, vol. 3: experimental results and evolutionary deductions.’ (University of Chicago Press: Chicago, IL)

Wright S (1978) ‘Evolution and the genetics of populations, vol. 4: variability within and among natural populations.’ (University of Chicago Press: Chicago, IL)

Yeh FC, Yang RC, Boyle T (1999) ‘POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31.’ (University of Alberta: Edmonton, Canada)


   
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