Transections of leaf blades from 46 C₄ grass species of known C₄ type were recorded for presence (XyMS+) or absence (XyMS-) of cells intervening between metaxylem vessel elements and laterally adjacent chlorenchymatous bundle sheath cells of primary lateral vascular bundles. A perfect correlation, only 'NADP-ME type' species being XyMS-, indicated that XyMS type serves as a useful predictor of C4 species type. The likely C4 type of 255 biochemically-untyped grass species was accordingly deduced by direct observation (94 spp.) or from Metcalf's anatomical descriptions (161 spp.). All 124 C4 species examined, including C₃ eu-panicoids, proved to be XyMS+.

Full text doi:10.1071/BT9760297

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