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RESEARCH ARTICLE
Contents Vol 65(4)

Towards the Development of a Direct Electrochemical Biodetector of Avidin Based on the Poly(chloro amino-β-styryl terthiophene)-Coated Glassy Carbon Electrode

Hakim Mehenni A C and Lê H. Dao B
+ Author Affiliations
- Author Affiliations

A Functional Nanomaterials Laboratory, Department of Materials Science, King Abdullah University of Science and Technology, Thuwal 23955, Kingdom of Saudi Arabia.

B Laboratoires de Recherche sur les Matériaux organiques Avancés, INRS-Energie et Matériaux, 1650 Bd Lionel Boulet, Varennes, Québec, Canada J3X 1S2.

C Corresponding author. Email: hakim.mehenni@kaust.edu.sa

Australian Journal of Chemistry 65(4) 395-401 https://doi.org/10.1071/CH11397
Submitted: 17 October 2011  Accepted: 19 February 2012   Published: 30 March 2012

Abstract

In this study, a simple and direct biodetector was proposed, which was based on biotin immobilized onto a conducting polymer-coated electrode, for the detection of avidin, a highly stable glycoprotein found in egg-whites. Biotin was immobilized onto the electrode by covalent coupling to the primary amine group on the poly 3′-(3-chloro-4-amino-β-styryl)-(2,2′ : 5′,2″-terthiophene) (PCAST), and the biotin–avidin interaction was monitored by cyclic voltammetry. Incubation of the PCAST/biotin-modified-coated electrode with avidin in a phosphate buffered saline solution caused a significant change to its cyclic voltammogram, which was explained by the binding of avidin by biotin, and resulted in restricted ion transfer to and from the conducting polymer. This change was then utilized to detect avidin at 4 × 10–6 mol L–1.


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