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Article << Previous     |     Next >>   Contents Vol 66(4)

A Colorimetric Aptamer Biosensor Based on Gold Nanoparticles for the Ultrasensitive and Specific Detection of Tetracycline in Milk

Lan He A D , Yanfang Luo B , Wenting Zhi C , Yuangen Wu A C and Pei Zhou B E

A School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
B School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
C Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, China.
D Bor S. Luh Food Safety Research Center, Shanghai 200240, China.
E Corresponding author. Email: zhoupei@sjtu.edu.cn

Australian Journal of Chemistry 66(4) 485-490 http://dx.doi.org/10.1071/CH12446
Submitted: 27 September 2012  Accepted: 5 December 2012   Published: 13 March 2013


 
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Abstract

This paper proposes a sensing strategy which employs an aptamer, unmodified gold nanoparticles (AuNP), and hexadecyltrimethylammonium bromide (CTAB) to detect tetracycline (TET) in raw milk. The method is based on the colorimetric assay of aggregating AuNP. In the absence of TET, the CTAB and aptamer form a complex which allows the aggregation of AuNP. In the presence of TET, the TET aptamer is exhausted first due to the formation of aptamer-TET complexes, which prevents assembly of the CTAB–aptamer supramolecule, causing a colour change and no aggregation of AuNP. This mechanism for the detection of TET proved to be sensitive and convenient. The colorimetric assay has a detection limit of 122 nM TET. This sensor has great potential for the sensitive, colorimetric detection of a wide range of molecular analytes.





References

[1]  M. Listgarten, J. Lindhe, L. Hellden, J. Clin. Periodontol. 1978, 5, 246.
         | CrossRef | CAS |

[2]  S. Rebe Raz, M. G. E. G. Bremer, W. Haasnoot, W. Norde, Anal. Chem. 2009, 81, 7743.
         | CrossRef | CAS |

[3]  X. Hu, J. Pan, Y. Hu, Y. Huo, G. Li, J. Chromatogr. A 2008, 1188, 97.
         | CrossRef | CAS |

[4]  V. Samanidou, S. Nisyriou, J. Sep. Sci. 2008, 31, 2068.
         | CrossRef | CAS |

[5]  D. J. Margolis, M. Fanelli, O. Hoffstad, J. D. Lewis, Am. J. Gastroenterol. 2010, 105, 2610.
         | CrossRef | CAS |

[6]  M. S. Hossain, M. A. Salam, G. Rabbani, I. Kabir, R. Biswas, D. Mahalanabis, J. Health Popul. Nutr. 2011, 20, 18.

[7]  Commission of the European Communities, Establishment by the European Community of Maximum Residue Limits (MRLs) for Residues of Veterinary Medical Products in Foodstuffs of Animal Origin, the Rules Governing Medical Products in the European Community, ECSC-EEC-EAEC, Brussels, 1991.

[8]  The Code of Federal Regulations, Title 21, Part 556, Section 152, 500, and 720. US Government Printing Office, Washington, DC, 2003.

[9]  J. Li, L. Chen, X. Wang, H. Jin, L. Ding, K. Zhang, H. Zhang, Talanta 2008, 75, 1245.
         | CrossRef | CAS |

[10]  V. Andreu, P. Vazquez-Roig, C. Blasco, Y. Picó, Anal. Bioanal. Chem. 2009, 394, 1329.
         | CrossRef | CAS |

[11]  N. Rodríguez, B. D. Real, M. Cruz Ortiz, L. A. Sarabia, A. Herrero, Anal. Chim. Acta 2009, 632, 42.
         | CrossRef |

[12]  N. E. Virolainen, M. G. Pikkemaat, J. W. A. Elferink, M. T. Karp, J. Agric. Food Chem. 2008, 56, 11065.
         | CrossRef | CAS |

[13]  A. Angelikaki, S. Girousi, Chem. Anal. (Pol.) 2008, 53, 445.
         | CAS |

[14]  M. Jeon, J. Kim, K. J. Paeng, S. W. Park, I. R. Paeng, Microchem. J. 2008, 88, 26.
         | CrossRef | CAS |

[15]  K. Sefah, J. A. Phillips, X. Xiong, L. Meng, D. Van Simaeys, H. Chen, J. Martin, W. Tan, Analyst 2009, 134, 1765.
         | CrossRef | CAS |

[16]  J. Liu, Z. Cao, Y. Lu, Chem. Rev. 2009, 109, 1948.
         | CrossRef | CAS |

[17]  C. Tuerk, L. Gold, Science 1990, 249, 505.
         | CrossRef | CAS |

[18]  A. D. Ellington, J. W. Szostak, Nature 1990, 346, 818.
         | CrossRef | CAS |

[19]  D. L. Robertson, G. F. Joyce, Nature 1990, 344, 467.
         | CrossRef | CAS |

[20]  A. D. Keefe, S. Pai, A. Ellington, Nat. Rev. Drug Discov. 2010, 9, 537.
         | CrossRef | CAS |

[21]  L. Gold, N. Janjic, T. Jarvis, D. Schneider, J. J. Walker, S. K. Wilcox, D. Zichi, Cold Spring Harb. Perspect. Biol. 2012, 4, 1.

[22]  M. Mascini, I. Palchetti, S. Tombelli, Angew. Chem. Int. Ed. 2012, 51, 1316.
         | CrossRef | CAS |

[23]  J. H. Niazi, S. J. Lee, M. B. Gu, Bioorg. Med. Chem. 2008, 16, 7245.
         | CrossRef | CAS |

[24]  T. Mairal, V. Cengiz Özalp, P. Lozano Sánchez, M. Mir, I. Katakis, C. K. O’Sullivan, Anal. Bioanal. Chem. 2008, 390, 989.
         | CrossRef | CAS |

[25]  H. J. Lee, B. C. Kim, M. K. Oh, J. Kim, Chem. Commun. 2012, 48, 5971.
         | CAS |

[26]  B. Li, H. Wei, S. Dong, Chem. Commun. 2007, 73.
         | CrossRef |

[27]  Y. Wu, S. Zhan, F. Wang, L. He, W. Zhi, P. Zhou, Chem. Commun. 2012, 48, 4459.
         | CrossRef | CAS |

[28]  L. He, W. Zhi, Y. Wu, S. Zhan, F. Wang, H. Xing, P. Zhou, Analytical Methods 2012, 4, 2266.
         | CAS |

[29]  J. Zhang, B. Zhang, Y. Wu, S. Jia, T. Fan, Z. Zhang, C. Zhang, Analyst 2010, 135, 2706.
         | CrossRef | CAS |

[30]  Y. J. Kim, Y. S. Kim, J. H. Niazi, M. B. Gu, Bioprocess Biosyst. Eng. 2010, 33, 31.
         | CrossRef | CAS |

[31]  I. Palchetti, M. Mascini, Analyst 2008, 133, 846.
         | CrossRef | CAS |

[32]  L. Li, B. Li, Y. Qi, Y. Jin, Anal. Bioanal. Chem. 2009, 393, 2051.
         | CrossRef | CAS |

[33]  Y. Wang, D. Li, W. Ren, Z. Liu, S. Dong, E. Wang, Chem. Commun. 2008, 2520.
         | CrossRef | CAS |

[34]  F. Xia, X. Zuo, R. Yang, Y. Xiao, D. Kang, A. Vallée-Bélisle, X. Gong, J. D. Yuen, B. B. Y. Hsu, A. J. Heeger, Proc. Natl. Acad. Sci. USA 2010, 107, 10837.
         | CrossRef | CAS |

[35]  D. K. Smith, B. A. Korgel, Langmuir 2008, 24, 644.
         | CrossRef | CAS |

[36]  P. Kannan, S. Sampath, S. A. John, J. Phys. Chem. C 2010, 114, 21114.
         | CAS |

[37]  H. Storchova, R. Hrdlickova, J. Chrtek, M. Tetera, D. Fitze, J. Fehrer, Taxon 2000, 49, 79.
         | CrossRef |

[38]  M. Singh, M. Ugozzoli, M. Briones, J. Kazzaz, E. Soenawan, D. T. O’Hagan, Pharm. Res. 2003, 20, 247.
         | CrossRef | CAS |

[39]  Y. Ofir, B. Samanta, V. M. Rotello, Chem. Soc. Rev. 2008, 37, 1814.
         | CrossRef | CAS |

[40]  D. Santhiya, S. Maiti, J. Phys. Chem. B 2010, 114, 7602.
         | CrossRef | CAS |

[41]  A. González-Pérez, R. S. Dias, T. Nylander, B. Lindman, Biomacromolecules 2008, 9, 772.
         | CrossRef |

[42]  Z. Jiang, X. Liao, A. Deng, A. Liang, J. Li, H. Pan, S. Wang, Y. Huang, Anal. Chem. 2008, 80, 8681.
         | CrossRef | CAS |

[43]  Y. Wu, S. Zhan, L. Xu, W. Shi, T. Xi, X. Zhan, P. Zhou, Chem. Commun. 2011, 47, 6027.
         | CAS |

[44]  J. Adrian, S. Pasche, G. Voirin, D. G. Pinacho, H. Font, F. Sánchez-Baeza, M. Marco, J.M. Diserens, B. Granier, TrAC Trends in Analytical Chem. 2009, 28, 769.
         | CAS |


   
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