CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Australian Journal of Chemistry   
Australian Journal of Chemistry
Journal Banner
  An international journal for chemical science
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
For Advertisers
Content
Online Early
Current Issue
Just Accepted
All Issues
Virtual Issues
Special Issues
Research Fronts
Sample Issue
Covers
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review Article
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

Affiliated with RACI

Royal Australian Chemical Institute
Royal Australian
Chemical Institute


 

Article << Previous     |     Next >>   Contents Vol 66(5)

Cytotoxicity In Vitro, Apoptosis, Cellular Uptake, Cell Cycle Distribution, Mitochondrial Membrane Potential Detection, DNA Binding, and Photocleavage of Ruthenium(ii) Complexes

Gan-Jian Lin A , Zheng-Zheng Li A , Jun-Hua Yao B , Hong-Liang Huang C D , Yang-Yin Xie A and Yun-Jun Liu A D

A School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
B Instrument Analysis and Research Center, Sun Yat-Sen Uiversity, Guangzhou, 510275, China.
C School of Life Science and Biopharmaceutical, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
D Corresponding authors. Email: hhongliang@163.com; lyjche@163.com

Australian Journal of Chemistry 66(5) 555-563 http://dx.doi.org/10.1071/CH12564
Submitted: 7 November 2012  Accepted: 7 January 2013   Published: 5 February 2013


 
PDF (1.6 MB) $25
 Export Citation
 Print
  
Abstract

Four new ruthenium(ii) complexes [Ru(bpy)2(NHPIP)](ClO4)2 (Ru-1), [Ru(phen)2(NHPIP)](ClO4)2 (Ru-2), [Ru(bpy)2(AHPIP)](ClO4)2 (Ru-3), and [Ru(phen)2(AHPIP)](ClO4)2 (Ru-4) (bpy = 2,2′-bipyridine; phen = 1,10-phenanthroline; NHPIP = 2-(3-nitro-4-hydroxylphenyl)imidazo[4,5-f][1,10]phenanthroline; AHPIP = 2-(3-amino-4-hydroxylphenyl)imidazo[4,5-f][1,10]phenanthroline) were synthesized and characterized by elemental analysis, electrospray mass spectrometry, and 1H NMR spectroscopy. The cytotoxicity in vitro of these complexes against BEL-7402, HeLa, MG-63, and MCF-7 cells was evaluated by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. Ru-4 shows the highest cytotoxic activity towards the selected cell lines among the four complexes. The morphological apoptosis was assayed by an acridine orange/ethidium bromide staining method, and the percentages of necrotic and apoptotic cells were determined by flow cytometry. The cellular uptake and the cell cycle arrest in BEL-7402 cell was investigated. The results showed these complexes inhibit the proliferation of BEL-7402 cells at G0/G1 phase arrest. The detection of mitochondrial membrane potentials using the fluorescence probe JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolcarbocyanine iodide) exhibited that the mitochondrial membrane potentials decrease. Upon irradiation, these complexes can effectively cleave pBR322 DNA.





References

[1]  K. E. Erkkila, D. T. Odom, J. K. Barton, Chem. Rev. 1999, 99, 2777.
         | CrossRef | CAS |

[2]  P. Bhattacharya, J. K. Barton, J. Am. Chem. Soc. 2001, 123, 8649.
         | CrossRef | CAS |

[3]  S. Delaney, J. Yoo, E. D. A. Stemp, J. K. Barton, Proc. Natl. Acad. Sci. USA 2004, 101, 10511.
         | CrossRef | CAS |

[4]  B. H. Yun, J. O. Kim, B. N. Lee, P. Lincoln, N. Norden, J. M. Kim, S. K. Kim, J. Phys. Chem. B 2003, 107, 9858.
         | CrossRef | CAS |

[5]  L. F. Tan, J. Liu, J. L. Shen, X. H. Liu, L. L. Zeng, L. H. Jin, Inorg. Chem. 2012, 51, 4417.
         | CrossRef | CAS |

[6]  U. Schatzschneider, J. Niesel, I. Ott, R. Gust, H. Alborzinia, S. Wölfl, ChemMedChem 2008, 3, 1104.
         | CrossRef | CAS |

[7]  D. D. Sun, Y. N. Liu, D. Liu, R. Zhang, X. C. Yang, J. Liu, Chem.–Eur. J. 2012, 18, 4285.
         | CrossRef | CAS |

[8]  B. S. Howerton, D. K. Heidary, E. C. Glazer, J. Am. Chem. Soc. 2012, 134, 8324.
         | CrossRef | CAS |

[9]  V. Rajendiran, M. Murali, E. Suresh, M. Palaniandavar, V. S. Periasamy, M. A. Akbarsha, Dalton Trans. 2008, 38, 2157.
         | CrossRef |

[10]  Y. J. Liu, C. H. Zeng, Z. H. Liang, J. H. Yao, H. L. Huang, Z. Z. Li, F. H. Wu, Eur. J. Med. Chem. 2010, 45, 3087.
         | CrossRef | CAS |

[11]  H. L. Huang, Z. Z. Li, Z. H. Liang, J. H. Yao, Y. J. Liu, Eur. J. Med. Chem. 2011, 46, 3282.
         | CrossRef | CAS |

[12]  Y. J. Liu, Z. H. Liang, Z. Z. Li, J. H. Yao, H. L. Huang, J. Organomet. Chem. 2011, 696, 2728.
         | CrossRef | CAS |

[13]  Y. J. Liu, Z. Z. Li, Z. H. Liang, J. H. Yao, H. L. Huang, DNA Cell Biol. 2011, 30, 839.
         | CrossRef | CAS |

[14]  W. Paw, R. Eisenberg, Inorg. Chem. 1997, 36, 2287.
         | CrossRef | CAS |

[15]  B. P. Sullivan, D. J. Salmon, T. J. Meyer, Inorg. Chem. 1978, 17, 3334.
         | CrossRef | CAS |

[16]  T. Mosmann, J. Immunol. Methods 1983, 65, 55.
         | CrossRef | CAS |

[17]  Cells: A Laboratory Manual (Eds D. L. Spector, R. D. Goldman, L. A. Leinwand) 1998, Vol. 1, Ch. 15 (Cold Spring Harbour Laboratory Press: New York, NY).

[18]  K. K. Lo, T. K. Lee, J. S. Lau, W. L. Poon, S. H. Cheng, Inorg. Chem. 2008, 47, 200.
         | CrossRef | CAS |

[19]  L. F. Tan, F. C. Song, X. Q. Zou, X. L. Ling, DNA Cell Biol. 2011, 30, 277.
         | CrossRef | CAS |

[20]  Q. F. Guo, S. H. Liu, Q. H. Liu, H. H. Xu, J. H. Zhao, H. F. Wu, X. Y. Li, J. W. Wang, DNA Cell Biol. 2012, 31, 1205.
         | CrossRef | CAS |

[21]  J. A. Hickman, Cancer Metastasis Rev. 1992, 11, 121.
         | CrossRef | CAS |

[22]  H. L. Huang, Z. Z. Li, Z. H. Liang, Y. J. Liu, Eur. J. Inorg. Chem. 2011, 36, 5538.
         | CrossRef |

[23]  C. T. Poon, P. S. Chan, C. Man, F. L. Jiang, R. N. S. Wong, N. K. Mak, D. W. J. Kwong, S. W. Tsao, W. K. Wong, J. Inorg. Biochem. 2010, 104, 62.
         | CrossRef | CAS |

[24]  O. Zava, S. M. Zakeeruddin, C. Danelon, H. Vogel, M. Grätzel, P. J. Dyson, ChemBioChem 2009, 10, 1796.
         | CrossRef | CAS |

[25]  J. K. Barton, A. L. Raphael, J. Am. Chem. Soc. 1984, 106, 2466.
         | CrossRef | CAS |


   
Subscriber Login
Username:
Password:  

 


    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014