Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data

Article

<< Previous

Next >>

Contents Vol 61(10)

doi:10.1071/CP10001

Plant Sciences, Sustainable Farming Systems & Food Quality

	Search




	Advanced Search



	Journal Home

	About the Journal

	New Editor-in-Chief

	Editorial Board

	Contacts



	Content

	Online Early

	Current Issue

	Just Accepted

	All Issues

	Special Issues

	Research Fronts

	Farrer Reviews

	Sample Issue



	For Authors

	General Information

	Notice to Authors

	Submit Article

	Open Access



	For Referees

	Referee Guidelines

	Review Article

	Annual Referee Index



	For Subscribers

	Subscription Prices

	Customer Service

	Print Publication Dates

e-Alerts

Subscribe to our Email Alert or

feeds for the latest journal papers.

Connect with us

Farrer Reviews

Invited Farrer Review Series. More...

PrometheusWiki

Protocols in ecological and environmental plant physiology

Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data

Dejan Dodig ^A ^F , Miroslav Zorić ^B , Borislav Kobiljski ^C , Gordana Šurlan-Momirović ^D and Steve A. Quarrie ^E

^A Maize Research Institute, Slobodana Bajica 1, 11185 Belgrade-Zemun Polje, Serbia.
^B Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
^C Institute of Field and Vegetative Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia.
^D Faculty of Agriculture, University of Belgrade, 11080 Belgrade-Zemun, Serbia.
^E School of Biology, Devonshire Building, Newcastle University, Newcastle-upon-Tyne, NE1 7RU, UK.
^F Corresponding author. Email: dejanza@yahoo.com

Crop and Pasture Science 61(10) 812-824 http://dx.doi.org/10.1071/CP10001
Submitted: 1 January 2010 Accepted: 30 July 2010 Published: 14 October 2010





	PDF (579 KB) $25



	Export Citation

Print

Abstract

This study was conducted to assess drought tolerance and regional-based patterns of diversity of bread wheat accessions and to identify new sources of diversity that could accelerate the development of improved wheat varieties better suited to meeting the challenges posed by changing climate in Southern and Eastern Europe. For this, genetic diversity assessed by simple sequence repeats (SSR) markers was compared with diversity evaluated using 19 phenotypic traits averaged over irrigated and drought-stress field conditions. Thirty-six SSR were used to profile 96 wheat genotypes from the collection of genetic resources at the Institute of Field and Vegetable Crops, Novi Sad, Serbia. A total of 46 loci and 366 alleles were detected, with a range of 3–21 alleles per locus. The polymorphic information content was estimated to be 0.61. The genetic distance for all possible 4560 pairs of genotypes ranged from 0.06 to 0.91 with an average of 0.65. Genotypes were grouped according to their drought tolerance (high, medium, low) and region of origin. Analysis of molecular variance showed that over 96% of the total variation could be explained by the variance within the drought tolerance and geographical groups. As a whole, genetic diversity among the high drought tolerance genotypes was considerably higher than that among low drought tolerance genotypes. Comparative analysis of SSR diversity among six regional groups revealed that the genotypes from North America exhibited more genetic diversity than those from other regions. Two dendrograms were constructed based on phenotypic and molecular analyses using the Unweighted Pair Group Method with Arithmetic Mean method and were found to be topologically different. Genotypes characterised as highly drought tolerant were distributed among all SSR-based cluster groups. This implied that the genetic basis of drought tolerance in these genotypes was different, thereby enabling wheat breeders to combine these diverse sources of genetic variability to improve drought tolerance in their breeding programs.

Additional keywords:bread wheat, drought tolerance, genetic diversity, phenotypic traits, SSR markers.

References

Ayana A, Bekele E (1998) Geographical patterns of morphological variation in sorghum (Sorghum bicolor (L.) Moench) germplasm from Ethiopia and Eritrea: qualitative characters. Hereditas 129, 195–205.
| CrossRef |

Bowcock AM, Ruiz Linares A, Tomfohrde J, Minch E, Kidd JR, Cavalli-Sforza LL (1994) High resolution of human evolutionary trees with polymorphic microsatellites. Nature 368, 455–457.
| CrossRef | CAS | PubMed |

Chao S, Zhang W, Dubcovsky J, Sorrells M (2007) Evaluation of genetic diversity and genome-wide linkage disequilibrium among U.S. wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Science 47, 1018–1030.
| CrossRef | CAS |

Dodig D, Quarrie SA, Stanković S, Milijić S, Denčić S (2002) Characterising wheat genetic resources for responses to drought stress. In ‘Proceedings of International Conference on Drought Mitigation and Prevention of Land Desertification’. Bled, Slovenia, 21–25 April 2002. (CD-ROM)

Dodig D, Zorić M, Knežević D, King SR, Šurlan-Momirović G (2008) Genotype × environment interaction for wheat yield in different drought stress conditions and agronomic traits suitable for selection. Australian Journal of Agricultural Research 59, 536–545.
| CrossRef |

El Mousadik A, Petit RJ (1996) High level of genetic differentiation for allelic richness among populations of the argan tree [Argania spinosa (L.) Skeels] endemic to Morocco. Theoretical and Applied Genetics 92, 832–839.
| CrossRef |

Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction sites. Genetics 131, 479–491.
| CAS | PubMed |

Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars: I. Grain yield responses. Australian Journal of Agricultural Research 29, 897–912.
| CrossRef |

Fufa H, Baenziger PS, Beecher BS, Dweikat I, Graybosh RA, Eskridge KM (2005) Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica 145, 133–146.
| CrossRef | CAS |

Gomez KA, Gomez AA (1984) ‘Statistical procedures for agricultural research.’ (John Wiley and Sons Inc.: New York)

Goudet J (2002) ‘FSTAT: a program to estimate and test gene diversities and fixation indices. Version 2.9.3.2.’ (Institut d’Ecologie, Laboratoire de Zoologie: Lausanne, Switzerland)

Gupta PK, Varshney RK, Sharma PC, Ramesh B (1999) Molecular markers and their applications in wheat breeding. Plant Breeding 118, 369–390.
| CrossRef | CAS |

Hai L, Wagner C, Friedt W (2007) Quantitative structure analysis of genetic diversity among spring bread wheats (Triticum aestivum L.) from different geographical regions. Genetica 130, 213–225.
| CrossRef | CAS | PubMed |

Hao CY, Zhang XY, Wang LF, Dong YS, Shang XW, Jia JZ (2006) Genetic diversity and core collection evaluations in common wheat germplasm from the Northwestern spring wheat region in China. Molecular Breeding 17, 69–77.
| CrossRef | CAS |

Hohls T (2001) Conditions under which selection for mean productivity, tolerance to environmental stress, or stability should be used to improve yield across a range of contrasting environments. Euphytica 120, 235–245.
| CrossRef |

Hopwood A, Oldroyd N, Fellows S, Ward R, Owen S-A, Sullivan K (1997) Rapid quantification of DNA samples extracted from buccal scrapes prior to DNA profiling. BioTechniques 23, 18–20.
| CAS | PubMed |

Huang XQ, Börner A, Röder MS, Ganal MW (2002) Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theoretical and Applied Genetics 105, 699–707.
| CrossRef | CAS | PubMed |

Khlestkina EK, Röder MS, Efremova TT, Börner A, Shumny VK (2004) The genetic diversity of old and modern Siberian varieties of common spring wheat as determined by microsatellite markers. Plant Breeding 123, 122–127.
| CrossRef | CAS |

Kobiljski B, Quarrie SA, Denčić S, Kirby J, Ivegeš M (2002) Genetic diversity of the Novi Sad wheat core collection revealed by microsatellites. Cellular & Molecular Biology Letters 7, 685–694.
| CAS | PubMed |

Koebner RMD, Donini P, Reeves J, Cooke RJ, Law JR (2003) Temporal flux in the morphological and molecular diversity of UK barley. Theoretical and Applied Genetics 106, 550–558.
| CAS | PubMed |

Lewis PO, Zaykin D (2001) ‘Genetic data analysis: computer program for the analysis of allelic data. Version 1.0.’ University of New Mexico: Alburquerque, NM)

Maccaferri M, Stefanelli S, Rotondo F, Tuberosa R, Sanguineti MC (2007) Relationships among durum wheat accessions. I. Comparative analysis of SSR, AFLP, and phenotypic data. Genome 50, 373–384.
| CrossRef | CAS | PubMed |

Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27, 209–220.
| CAS | PubMed |

Martínez L, Cavagnaro P, Masuelli R (2005) Evaluation of diversity among Argentine grapevine (Vitis vinifera L.) varieties using morphological data and AFLP markers. Electronic Journal of Biotechnology 6, 37–45.

Minch E, Ruiz-Linares A, Goldstein D, Feldman M, Cavalli-Sforza LL (1997) ‘Microsat: a computer program for calculating various statistics on microsatellite allele data, Version1.5d.’ (Stanford University: Stanford, CA)

Moghaddam ME, Trethowan RM, William HM, Rezai A, Arzani A, Mirlohi AF (2005) Assessment of genetic diversity in bread wheat genotypes for tolerance to drought using AFLPs and agronomic traits. Euphytica 141, 147–156.
| CrossRef | CAS |

Noli E, Teriaca MS, Sanguineti MC, Conti S (2008) Utilization of SSR and AFLP markers for the assessment of distinctness in durum wheat. Molecular Breeding 22, 301–313.
| CrossRef | CAS |

Prasad B, Babar MA, Xu XY, Bai GH, Klatt AR (2009) Genetic diversity in U.S. hard red winter wheat cultivars as revealed by microsatellite markers. Crop & Pasture Science 60, 16–24.
| CrossRef | CAS |

Prasad M, Varshney RK, Roy JK, Balayan BS, Gupta PK (2000) The use of microsatellites for detecting DNA polymorphism, genotype identification and genetic diversity in wheat. Theoretical and Applied Genetics 100, 584–592.
| CrossRef | CAS |

Quarrie SA, Dodig D, Pekić S, Kirby J, Kobiljski B (2003) Prospects for marker-assisted selection of improved drought responses in wheat. Bulgarian Journal of Plant Physiology Special Issue, 83–95.

R Development Core Team (2009) ‘R: a language and environment for statistical computing.’ (R Foundation for Statistical Computing: Vienna, Austria) Available at: www.R-project.org

Rajaram S, Braun HJ, van Ginkel M (1996) CIMMYT’s approach to breed for drought tolerance. Euphytica 92, 147–153.
| CrossRef |

Rebourg C, Gouesnard B, Charcosset A (2001) Large scale molecular analysis of traditional European maize populations. Relationships with morphological variation. Heredity 86, 574–587.
| CrossRef | CAS | PubMed |

Reynolds MP, Mujeeb-Kazi A, Sawkins M (2005) Prospects of utilizing plant adaptive mechanisms to improve wheat and other crops in drought and salinity prone environments. Annals of Applied Biology 146, 239–259.
| CrossRef | CAS |

Rice WR (1989) Analyzing tables of statistical tests. Evolution 43, 223–225.
| CrossRef |

Robertson A (1959) The sampling variance of the genetic correlation coefficient. Biometrics 15, 469–485.
| CrossRef |

Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |

Rohlf FJ (2000) ‘NTSYS-pc. Numerical taxonomy and multivariate analysis system, version 2.1.’ (Exeter Publications: New York)

Rosielle AA, Hamblin J (1981) Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science 21, 943–946.
| CrossRef |

Roussel V, Koenig J, Beckert M, Balfourier F (2004) Molecular diversity in French bread wheat accessions related to temporal trends and breeding programmes. Theoretical and Applied Genetics 108, 920–930.
| CrossRef | CAS | PubMed |

Roussel V, Leisova L, Exbrayat F, Stehno Z (2005) SSR allelic diversity changes in 480 European bread wheat varieties released from 1840 to 2000. Theoretical and Applied Genetics 111, 162–170.
| CrossRef | CAS | PubMed |

Rousset F (2008) Genepop’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8, 103–106.
| CrossRef |

Schneider S, Roessli D, Excoffier L (2000) ‘ARLEQUIN Version 2.000: a software for population genetic data analysis.’ (Genetics and Biometry Laboratory, University of Geneva: Geneva, Switzerland)

Sio-Se Mardeh A, Ahmadi A, Poustini K, Mohammadi V (2006) Evaluation of drought resistance indices under various environmental conditions. Field Crops Research 98, 222–229.
| CrossRef |

Spasov P, Spasov D, Petrović P (2002) Changes in drought occurrences in Serbia. In ‘Proceedings of International Conference on Drought Mitigation and Prevention of Land Desertification’. Bled, Slovenia, 21–25 April 2002. (CD-ROM) (International Commission on Irrigation and Drainage (ICID): Ljubljana, Slovenia)

Stephenson P, Bryan G, Kirby J, Collins A, Devos K, Busso C, Gale M (1998) Fifty new microsatellite loci for the wheat genetic map. Theoretical and Applied Genetics 97, 946–949.
| CrossRef | CAS |

Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24, 1596–1599.
| CrossRef | CAS | PubMed |

van Beuningen LT, Busch RH (1997) Genetic diversity among North American spring wheat cultivars: III. Cluster analysis based on quantitative morphological traits. Crop Science 37, 981–988.
| CrossRef |

Weir BS, Hill WG (2002) Estimating F-statistics. Annual Review of Genetics 36, 721–750.
| CrossRef | CAS | PubMed |

Yeh FC, Boyle TJ (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany 129, 157–163.

Yifru T, Hammer K, Huang XQ, Röder MS (2006) Regional patterns of microsatellite diversity in Ethiopian tetraploid wheat accession. Plant Breeding 125, 125–130.
| CrossRef | CAS |

Zhang XY, Li CW, Wang LF, Wang HM, You GX, Dong YS (2002) An estimation of the minimum number of SSR alleles needed to reveal genetic relationships in wheat varieties. I. Information from large-scale planted varieties and cornerstone breeding parents in Chinese wheat improvement and production. Theoretical and Applied Genetics 106, 112–117.
| CAS | PubMed |

Subscriber Login

	Username: Password:

Legal & Privacy | Contact Us | Help