On the role of H2O2 in the recovery of grapevine (Vitis vinifera cv. Prosecco) from Flavescence dorée disease
Rita Musetti A F , Rosita Marabottini B , Maurizio Badiani C , Marta Martini A , Luigi Sanità di Toppi D , Stefano Borselli A , Michele Borgo E and Ruggero Osler AA Dipartimento di Biologia Applicata alla Difesa delle Piante, Università di Udine, via delle Scienze 208, I-33100 Udine, Italy.
B Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Via S.C. De Lellis, I-01100 Viterbo, Italy.
C Dipartimento di Biotecnologie per il Monitoraggio Agro-Alimentare ed Ambientale, Università Mediterranea di Reggio Calabria, Facoltà di Agraria, I-89129 Reggio Calabria, Italy.
D Dipartimento di Biologia Evolutiva e Funzionale, Sezione di Biologia Vegetale, Università di Parma, viale G. P. Usberti 11/A, I-43100 Parma, Italy.
E CRA-Istituto Sperimentale per la Viticoltura, Conegliano, Italy.
F Corresponding author. Email: rita.musetti@uniud.it
Functional Plant Biology 34(8) 750-758 https://doi.org/10.1071/FP06308
Submitted: 17 November 2006 Accepted: 22 May 2007 Published: 23 July 2007
Abstract
In the present work, we compared hydrogen peroxide (H2O2) localisation and the activities/contents of antioxidant enzymes and metabolites in the leaf tissues of grapevine (Vitis vinifera L. cv. Prosecco) plants showing different sanitary status, namely diseased by Flavescence dorée, healthy or recovered. Polymerase chain reaction analysis revealed that the pathogen associated with Flavescence dorée (proposed as ‘Candidatus Phytoplasma vitis’) was detected in the leaf tissues of symptomatic plants, but was not observed in either the healthy or recovered plants. Hydrogen peroxide accumulated in the phloem plasmalemma of recovered grapevine leaves, but was not detected in either healthy or diseased material. When compared to diseased or healthy plants, recovered plants had distinctly lower extractable levels of catalase and ascorbate peroxidase, two enzymes primarily involved in the scavenging of excess H2O2 generated in different cell compartments. Among healthy, diseased and recovered leaves there was no significant difference in the amount of 2-thiobarbituric acid-reactive substances, which are assumed to reflect the extent of peroxidative breakdown of membrane lipids. Therefore, it is suggested that recovery from Flavescence dorée disease in grapevine might be associated with a long-term, sustained and tissue-specific accumulation of H2O2 in leaves, which reduces numbers or prevents further infection by Flavescence dorée phytoplasma. Recovered grapevine plants might be able to achieve such H2O2 accumulation through a selective and presumably stable downregulation of enzymatic H2O2 scavengers, without altering the levels of other antioxidant systems and without incurring an increased oxidative risk.
Additional keywords: antioxidants, hydrogen peroxide, phytoplasma.
Acknowledgements
This research was funded by the Italian Ministry for Agriculture and Forestry, finalised project ‘I giallumi della vite: un fattore limitante per le produzioni vitivinicole’, and by the Italian Ministry for University and Research, PRIN Project, 2005, Rome. The authors are grateful to Dr Laurence Cantrill for English text revision.
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