The objectives of this research were to investigate the suitability of advanced technologies like 3D-Laser scanning to acquire fair and sound information on structural and architectural characteristics of poplar stand, and to map topology of above-ground tree structures. The study area was an intensive poplar plantation located ~10 km north-west of the city of Pavia within the ‘Parco Regionale del Ticino’, Italy. A forest inventory of the poplar stand was conducted in 2005 and three 14-year-old poplar trees were selected and felled. The main architectural characteristics of poplar trees (destructive measurement) were compared with indirect measurement carried out using a portable on-ground scanning LIDAR IMAGER 5003 combined with the JRC-Reconstructor and AMAPmod softwares. The method permitted us to make an accurate estimate of the vertical and horizontal structure of the stand, to evaluate the stem and branches morphology of selected trees at different height in the canopy, and to create and validate multiscale representations of poplar tree architecture.