Is mesophyll conductance to CO2 in leaves of three Eucalyptus species sensitive to short-term changes of irradiance under ambient as well as low O2?

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doi:10.1071/FP11190

Plant Function & Evolutionary Biology

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Protocols in ecological and environmental plant physiology

Is mesophyll conductance to CO₂ in leaves of three Eucalyptus species sensitive to short-term changes of irradiance under ambient as well as low O₂?

Cyril Douthe ^A ^B ^C, Erwin Dreyer ^A ^B ^D, Oliver Brendel ^A ^B and Charles R. Warren ^C

^A INRA, Unité Mixte de Recherches 1147 ‘Ecologie et Ecophysiologie Forestières’, F 54280 Champenoux, France.
^B Université de Lorraine, Unité Mixte de Recherches 1147 ‘Ecologie et Ecophysiologie Forestières’, Faculté des Sciences, F 54500 Vandoeuvre, France.
^C University of Sydney, School of Biological Sciences, Heydon-Laurence Building, A08, The University of Sydney, NSW 2006, Australia.
^D Corresponding author. Email: dreyer@nancy.inra.fr

Functional Plant Biology 39(5) 435-448 http://dx.doi.org/10.1071/FP11190
Submitted: 25 August 2011 Accepted: 26 March 2012 Published: 11 May 2012





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Abstract

Mesophyll conductance to CO₂ (g _m) limits the diffusion of CO₂ to the sites of carboxylation, and may respond rapidly (within minutes) to abiotic factors. Using three Eucalyptus species, we tested the rapid response of g _m to irradiance under 21% and 1% O₂. We used simultaneous measurements of leaf gas exchange and discrimination against ¹³CO₂ with a tuneable diode laser absorption spectrometer. Measurements under 1% O₂ were used to limit uncertainties due to ¹³C–¹²C fractionation occurring during photorespiration. Switching irradiance from 600 to 200 µmol m^–2 s^–1 led to a ≈60% decrease of g _m within minutes in all species under both 21% O₂ and 1% O₂. The g _m response to irradiance is unlikely to be a computation artefact since using different values for the parameters of the discrimination model changed the absolute values of g _m but did not affect the relative response to irradiance. Simulations showed that possible rapid changes of any parameter were unable to explain the observed variations of g _m with irradiance, except for¹³C–¹²C fractionation during carboxylation (b), which, in turn, is dependent on the fraction of leaf C assimilated by phospho-enol pyruvate carboxylase (PEPc) (β). g _m apparently increased by ≈30% when O₂ was switched from 21% to 1% O₂. Again, possible changes of β with O₂ could explain this apparent g _m response to O₂. Nevertheless, large irradiance or O₂-induced changes in β would be required to fully explain the observed changes in g _m, reinforcing the hypothesis that g _m is responsive to irradiance and possibly also to O₂.

Additional keywords: ¹³C discrimination, photorespiration, photosynthesis, stomatal conductance, tuneable diode laser absorption spectrometry.

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