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Open Access Article     |     Next >>   Contents Vol 28(5)

Integrative taxonomy of Acrapex stem borers (Lepidoptera : Noctuidae : Apameini): combining morphology and Poisson Tree Process analyses

Bruno P. Le Ru A B N, Claire Capdevielle-Dulac B, Emmanuel F. A. Toussaint C, Desmond Conlong D E, Johnnie Van den Berg F, Beatrice Pallangyo G, George Ong’amo H, Gilson Chipabika I, Richard Molo J, William A. Overholt K, James P. Cuda L and Gael J. Kergoat M

A Unité de Recherche IRD 072, icipe-African Insect Science for Food and Health, PO Box 30772, Nairobi, Kenya.
B Unité de Recherche IRD 072, Laboratoire Evolution, Génomes et Spéciation, UPR 9034, 22 CNRS, 91198 – Gif/Yvette, France and Université Paris-Sud 11, 91405 – Orsay, France.
C Zoological State Collection, Münchhausenstraße 21, 81247 – Munich, Germany.
D South African Sugarcane Research Institute, Private Bag X02, Mount Edgecombe, 4300, South Africa.
E School of Biological and Conservation Sciences, University of Kwazulu–Natal, Private Bag X01, Scottsville, Pietermaritzburg, South Africa.
F School of Environmental Sciences and Development, North West University (Potchefstroom Campus), Private Bag X6001, Potchefstroom, 2520, South Africa.
G Biocontrol Programme, PO Box 30031, Kibaha, Tanzania.
H School of Biological Science, College of Physical and Biological Sciences (Chiromo Campus), University of Nairobi, P. O. Box 30197, Nairobi, Kenya.
I Zambia Agriculture Research Institute, Mount Maluku Central Research Station, PO Box 8, Chilanga, Zambia.
J Namulonge Agricultural and Animal Production Research Institute (NAARI), PO Box 7084, Kampala, Uganda.
K Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945, USA.
L Department of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USA.
M INRA, UMR 1062 CBGP (INRA, IRD, CIRAD, Montpellier SupAgro, Campus de Baillarguet, 34988 Montferrier/Lez, France.
N Corresponding author. Email: bleru@icipe.org

Invertebrate Systematics 28(5) 451-475 http://dx.doi.org/10.1071/IS13062
Submitted: 17 December 2013  Accepted: 16 May 2014   Published: 13 November 2014

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Ten morphologically similar species of Acrapex from eastern and south-eastern Africa belonging to the A. stygiata and A. albivena groups are reviewed. Six species are described as new: A. brunneella, A. mitiwa, A. mpika, A. salmona, A. sporobola and A. yakoba. The Poaceae host plants of eight species are recorded; four species, A. mitiwa. A. subalbissima, A. syscia and A. yakoba, were found developing exclusively on Imperata cylindrica (L.) Beauv., (Andropogoneae); two species, A. sporobola and A. salmona, on I. cylindrica and Sporobolus macranthelus Chiov. (Zoysieae); and A. albivena on I. cylindrica, Miscanthus capensis (Nees) Andersson (Andropogoneae) and Cymbopogon sp. (Andropogoneae). Acrapex stygiata larvae developed on M. capensis and Cymbopogon sp. The host plants of A. brunneella and A. mpika remain unknown. We also conducted molecular phylogenetics and molecular species delimitation analyses on a comprehensive sample of 49 specimens belonging to nine of the studied species. Molecular phylogenetics and molecular species delimitation analyses provided additional evidence of the validity of the six newly described species but also suggested a level of hidden biodiversity for one of them.

Additional keywords: Acrapex, Imperata cylindrica, molecular phylogenetics, molecular species delimitation, Noctuidae, phylogenetics, Poaceae.


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