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Developmental exposure to Passiflora incarnata induces behavioural alterations in the male progeny

André D. Bacchi A, Bianca Ponte A, Milene L. Vieira A, Jaqueline C. C. de Paula A, Suzana F. P. Mesquita B, Daniela C. C. Gerardin A and Estefânia G. Moreira A C

A Department of Physiological Sciences, State University of Londrina, 86051-980, Londrina, Paraná, Brazil.
B Department of Biology, State University of Londrina, 86051-980, Londrina, Paraná, Brazil.
C Corresponding author. Email: egmoreira@uel.br

Reproduction, Fertility and Development - http://dx.doi.org/10.1071/RD11307
Submitted: 12 December 2011  Accepted: 25 June 2012   Published online: 8 August 2012


 
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Abstract

Passiflora incarnata is marketed in many countries as a phytomedicine and is prescribed mainly as a sedative and anxiolytic. Even though the directions of most marketed phytomedicines recommend them to be used under medical supervision, reproductive and developmental studies are sparse and not mandatory for regulatory purposes. To evaluate the reproductive and developmental toxicity of P. incarnata, Wistar female rats were gavaged with 30 or 300 mg kg–1 of this herb from gestational Day (GD) 0 to postnatal Day (PND) 21. P. incarnata treatment did not influence dams’ bodyweight or food intake or their reproductive performance (post-implantation loss, litter size, litter weight). There was also no influence on the physical development of pups (bodyweight gain, day of vaginal opening or preputial separation) or their behaviour in the open-field at PND 22, 35 and 75. Sexual behaviour was disrupted in adult male pups exposed to 300 mg kg–1 of P. incarnata; in this group, only 3 out of 11 pups were sexually competent. This behavioural disruption was not accompanied by alterations in plasma testosterone levels, reproductive-related organs and glands weights or sperm count. It is hypothesised that aromatase inhibition may be involved in the observed effect.

Additional keywords: developmental toxicity, lactation, open-field, pregnancy, reproductive toxicity, sexual behaviour.


References

Agmo, A. (1997). Male rat sexual behavior. Brain Res. 1, 203–209.
| CAS |

Agmo, A. (2003). Lack of opioid or dopaminergic effects on unconditioned sexual incentive motivation in male rats. Behav. Neurosci. 117, 55–68.
CrossRef | CAS |

Aste, N., Honda, S., and Harada, N. (2003). Forebrain Fos responses to reproductively related chemosensory cues in aromatase knockout mice. Brain Res. Bull. 60, 191–200.
CrossRef | CAS |

Attila, M., Oksala, R., and Agmo, A. (2010). Sexual incentive motivation in male rats requires both androgens and oestrogens. Horm. Behav. 58, 341–351.
CrossRef | CAS |

Bakker, J., Brand, T., Van Ophemert, J., and Slob, A. K. (1993). Hormonal regulation of adult partner preference behavior in neonatally ATD-treated male rats. Behav. Neurosci. 107, 480–487.
CrossRef | CAS |

Bakker, J., Van Ophemert, J., and Slob, A. K. (1996). Sexual differentiation of odour and partner preference in rat. Physiol. Behav. 60, 489–494.
CrossRef | CAS |

Barros, D., Amaral, O., Izquierdo, I., Geracitano, L., Raseira, M. C. B., Henriques, A. T., and Ramirez, M. R. (2006). Behavioural and genoprotective effects of Vaccinium berries intake in mice. Pharmacol. Biochem. Behav. 84, 229–234.
CrossRef | CAS |

Baum, M. J., Carroll, R. S., and Tobet, S. A. (1990). Steroidal control of behavioural, neuroendocrine and brain sexual differentiation. J. Neuroendocrinol. 2, 401–418.
CrossRef | CAS |

Beyer, B. K., Chernoff, N., Danielsson, B. R., Davis-Bruno, K., Harrouk, W., and Hood, R. D. (2011). ILSI/HESI maternal toxicity workshop summary: maternal toxicity and its impact on study design and data interpretation. Birth Defects Res. B Dev. Reprod. Toxicol. 92, 36–51.
CrossRef | CAS |

Booth, J. E. (1977). Sexual behaviour of neonatally castrated rats injected during infancy with oestrogen and dihydrotestosterone. J. Endocrinol. 72, 135–141.
CrossRef | CAS |

BRASIL (2008). Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Resolução de Diretoria Colegiada no 95, de 11 de dezembro de 2008. Regulamenta o texto de bulas de medicamentos fitoterápicos. Available at http://189.28.128.100/dab/docs/legislacao/resolucao95_11_12_08.pdf [accessed September 2011].

Broadhurst, P. L. (1960). Experiments in psychogenetics. In ‘Experiments in Personality’. (Ed. H. J. Eysenk.) pp. 31–61. (Rutledge and Kegan Paul: London.)

Buratti, F. M., De Angelis, G., Ricceri, L., Venerosi, A., Calamandrei, G., and Testai, E. (2011). Foetal and neonatal exposure to chlorpyrifos: biochemical and metabolic alterations in the mouse liver at different developmental stages. Toxicology 280, 98–108.
CrossRef | CAS |

Buttar, H. S., and Jones, K. L. (2003). What do we know about the reproductive and developmental risks of herbal and alternate remedies? Birth Defects Res. B Dev. Reprod. Toxicol. 68, 492–493.
CrossRef | CAS |

Dhawan, K., Kumar, S., and Sharma, A. (2002). Suppression of alcohol-cessation-oriented hyper-anxiety by the benzoflavone moiety of Passiflora incarnata Linneaus in mice. J. Ethnopharmacol. 81, 239–244.
CrossRef | CAS |

Dhawan, K., Dhawan, S., and Sharma, A. (2004). Passiflora: a review update. J. Ethnopharmacol. 94, 1–23.
CrossRef | CAS |

Feder, H. H., and Whalen, R. E. (1965). Feminine behaviour in neonatally castrated and oestrogen-treated male rats. Science 147, 306–307.
CrossRef |

Freeman, L. M., and Rissman, E. F. (1996). Neural aromatisation and the control of sexual behaviour. Trends Endocrinol. Metab. 7, 334–338.
CrossRef | CAS |

Gerardin, D. C. C., Piffer, R. C., Garcia, P. C., Moreira, E. G., and Pereira, O. C. M. (2008). Effects of maternal exposure to an aromatase inhibitor on sexual behaviour and neurochemical and endocrine aspects of adult male rat. Reprod. Fertil. Dev. 20, 557–562.
CrossRef | CAS |

Grundmann, O., Wang, J., McGregor, G. P., and Butterweck, V. (2008). Anxiolytic activity of a phytochemically characterized Passiflora incarnata extract is mediated via the GABAergic system. Planta Med. 74, 1769–1773.
CrossRef | CAS |

ICH (2005). Detection of toxicity to reproduction for medicinal products & toxicity to male fertility S5(R2). International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use.

Jones, M. E. E., Boon, W. C., Proietto, J., and Simpson, E. R. (2006). Of mice and men: the evolving phenotype of aromatase deficiency. Trends Endocrinol. Metab. 17, 55–64.
CrossRef |

Koren, G., Dugoua, J. J., Magee, L., Vohra, S., Matsui, D., Bérard, A., Johnson, B., Moretti, M., and Einarson, A. (2008). Mother nature: establishing a Canadian research network for natural health products (NHPs) during pregnancy and lactation. J. Altern. Complement. Med. 14, 369–372.
CrossRef |

Moreira, E. G., Vassilieff, I., and Vassilieff, V. S. (2001). Developmental lead exposure: behavioural alterations in the short and long term. Neurotoxicol. Teratol. 23, 489–495.
CrossRef | CAS |

Nordeng, H., and Havnen, G. C. (2005). Impact of socio-demographic factors, knowledge and attitude on the use of herbal drugs in pregnancy. Acta Obstet. Gynecol. Scand. 84, 26–33.

Pellow, S., and File, S. E. (1986). Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze: a novel test of anxiety in the rat. Pharmacol. Biochem. Behav. 24, 525–529.
CrossRef | CAS |

Refuerzo, J. S., Blackwell, S. C., and Sokol, R. J. (2005). Use of over-the-counter medication and herbal remedies in pregnancy. Am. J. Perinatol. 22, 321–324.
CrossRef |

Robb, G. W., Amann, R. P., and Killian, G. J. (1978). Daily sperm production and epididymal sperm reserves of pubertal and adult rats. J. Reprod. Fertil. 54, 103–107.
CrossRef | CAS |

Roselli, C. E., Cross, E., Poonyagariyagorn, H. K., and Stadelman, H. L. (2003). Role of aromatisation in anticipatory and consummatory aspects of sexual behaviour in male rats. Horm. Behav. 44, 146–151.
CrossRef | CAS |

Ruggy, G. H., and Smith, C. S. (1940). A pharmacological study of the active principle of Passiflora incarnata. J. Am. Pharm. Assoc. 29, 245–249.
| CAS |

Soulimani, R., Younos, C., Jarmouni, S., Bousta, D., Misslin, R., and Mortier, F. (1997). Behavioural effects of P. incarnata L. and its indole alkaloid and flavonoid derivatives and maltol in the mouse. J. Ethnopharmacol. 57, 11–20.
CrossRef | CAS |

Tiran, D. (2003). The use of herbs by pregnant and childbearing women: a risk–benefit assessment. Complement. Ther. Nurs. Midwifery 9, 176–181.
CrossRef |

Veenema, A. H., Bredewold, R., and Neumann, I. D. (2007). Opposite effects of maternal separation on inter-male and maternal aggression in C57BL/6 mice: link to hypothalamic vasopressin and oxytocin immunoreactivity. Psychoneuroendocrinology 32, 437–450.
CrossRef | CAS |

Veríssimo, L.F., Bacchi, A.D., Zaminelli, T., De Paula, G.H.O., and Moreira, E.G. (2011). Herbs of interest to the Brazilian Federal Government: female reproductive and developmental toxicity studies. Rev. Bras. Farmacogn , .
CrossRef |

Vianna, M. R. M., Izquierdo, L. A., Barros, D. M., Souza, M. M., Rodrigues, C., Sant’Anna, M. K., Medina, J. H., and Izquierdo, I. (2001). Pharmacological differences between memory consolidation of habituation to an open field and inhibitory avoidance learning. Braz. J. Med. Biol. Res. 34, 233–240.
CrossRef | CAS |

Yunes, R. A., and Calixto, J. B. (2001). ‘Plantas Medicinais: Sob a Ótica da Química Medicinal Moderna’. 1st edn. (Argos Editora Universitária: Chapecó.)

Zanoli, P., Avallone, R., and Baraldi, M. (2000). Behavioural characterisation of the flavonoids apigenin and chrysin. Fitoterapia 71, S117–S123.
CrossRef | CAS |


   
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