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Article << Previous     |     Next >>   Contents Vol 25(5)

Persistence of an immunoreactive MUC1 protein at the feto–maternal interface throughout pregnancy in the mare

Sandra Wilsher A B , Sue Gower A and W. R. Allen A

A The Paul Mellon Laboratory of Equine Reproduction, ‘Brunswick’, 18 Woodditton Road, Newmarket, Suffolk CB8 9BJ, UK.
B Corresponding author. Email: pml@btinternet.com

Reproduction, Fertility and Development 25(5) 753-761 http://dx.doi.org/10.1071/RD12152
Submitted: 11 May 2012  Accepted: 19 June 2012   Published: 8 August 2012


 
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Abstract

A polyclonal human mucin-1 (MUC1) antibody was used to stain immunohistochemically for the presence of MUC1 on the endometrium and fetal membranes in mares between 20 and 309 days of gestation. Western blot analysis demonstrated the presence of a protein equivalent in size to a human MUC1 isoform, MUC1/Y, in equine endometrium, allantochorion and amnion. At all stages of gestation examined immunoreactivity to the MUC1 antibody was detected on the apical surface of the lumenal epithelium of the endometrium and the epithelium lining the mouths and apical regions of the endometrial glands. Furthermore, it persisted unchanged on the surface of the lumenal epithelium lying beneath the highly-invasive chorionic girdle component of the trophoblast before, during and after development of the endometrial cups. The MUC1 immunoreactive protein was also present on the trophoblast and other components of the fetal membranes during the post-fixation, pre-attachment period of gestation (20–40 days) and it persisted on the apical surface of the non-invasive trophoblast of the allantochorion before, during and after attachment, microvillous interdigitation and development of the microcotyledonary epitheliochorial placenta. Hence, the delayed placentation response in mares appears to occur independently of the persistence of an immunoreactive MUC1 protein at the feto–maternal interface.

Additional keywords: endometrium, MUC1/Y, trophoblast.


References

Allen, W. R. (1969). The immunological measurement of pregnant mare serum gonadotrophin. J. Endocr. 43, 593–598.
CrossRef | CAS | PubMed |

Allen, W. R., Hamilton, D. W., and Moor, R. M. (1973). The origin of the equine endometrial cups. II. Invasion of the endometrium by trophoblast. Anat. Rec. 177, 485–501.
CrossRef | CAS | PubMed |

Aplin, J. D., Seif, M. W., Graham, R. A., Hey, N. A., Behzad, F., and Campbell, S. (1994). The endometrial cell surface and implantation. Expression of the polymorphic mucin, MUC1, and adhesion molecules during the endometrial cycle. Ann. N. Y. Acad. Sci. 734, 103–121.
CrossRef | CAS | PubMed |

Baruch, A., Hartmann, M.-L., Zrihan-Licht, S., Greenstein, S., Burstein, M., Keydar, I., Weiss, M., Smorodinsky, N., and Wreschner, D. H. (1997). Preferential expression of novel MUC1 tumour antigen isoforms in human epithelial tumours and their tumour-potentiating function. Int. J. Cancer 71, 741–749.
CrossRef | CAS | PubMed |

Baruch, A., Hartmann, M.-L., Yoeli, M., Adereth, Y., Greenstein, S., Stadler, Y., Skornik, Y., Zaretsky, J., Smorodinsky, N. I., Keydar, I., and Wreschner, D. H. (1999). The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein. Cancer Res. 59, 1552–1561.
| CAS | PubMed |

Battut, I., Colchen, S., Fiéni, F., Tainturier, D., and Bruyas, J. F. (1997). Success rates when attempting to non-surgically collect equine embryos at 144, 156 and 168 hours after ovulation. Equine Vet. J. Suppl. 25, 60–62.
| PubMed |

Bazer, F. W., Spencer, T. E., Johnson, G. A., Burghardt, R. C., and Wu, G. (2009). Comparative aspects of implantation. Reproduction 138, 195–209.
CrossRef | CAS | PubMed |

Betteridge, K. J. (1989). The structure and function of the equine capsule in relation to embryo manipulation and transfer. Equine Vet. J. Suppl. 8, 92–100.

Bowen, J. A., Bazer, F. W., and Burghardt, R. C. (1997). Spatial and temporal analyses of integrin and Muc-1 expression in porcine uterine epithelium and trophectoderm in vitro. Biol. Reprod. 56, 409–415.
CrossRef | CAS | PubMed |

Braga, V. M. M., and Gendler, S. J. (1993). Modulation of Muc-1 expression in the mouse uterus during the oestrus cycle, early pregnancy and placentation. J. Cell Sci. 105, 397–405.
| CAS |

Brayman, M., Thathiah, A., and Carson, D. D. (2004). MUC1: a multifunctional cell surface component of reproductive tissue epithelia. Reprod. Biol. Endocrinol. 2, 4–13.
CrossRef | PubMed |

Carson, D. D., Wilson, O. F., and Dutt, A. (1990). Glycoconjugate expression and interactions at the cell surface of mouse uterine epithelial cells and peri-implantation stage embryos. Trophoblast Res. 4, 211–241.
| CAS |

Carson, D. D., DeSouza, M. M., Kardon, R., Zhou, X., Lagow, E., and Julian, J. A. (1998). Mucin expression and function in the female reproductive tract. Hum. Reprod. Update 4, 459–464.
CrossRef | CAS | PubMed |

Carson, D. D., Julian, J., Lessey, B. A., Prakobphol, A., and Fisher, S. J. (2006). MUC1 is a scaffold for selectin ligands in the human uterus. Front. Biosci. 11, 2903–2908.
CrossRef | CAS | PubMed |

Daels, P. F., DeMoraes, J. J., Stabenfeldt, G. H., Hughes, J. P., and Lasley, B. L. (1991). The corpus luteum: source of oestrogen during early pregnancy in the mare. J. Reprod. Fertil. Suppl. 35, 501–508.

DeLoia, J. A., Krasnow, J. S., Brekosky, J., Babaknia, A., Julian, J., and Carson, D. D. (1998). Regional specialisation of MUC1 in human uterine epithelia. Hum. Reprod. 13, 2902–2909.
CrossRef | CAS | PubMed |

DeSouza, M. M., Mani, S., Julian, J., and Carson, D. D. (1998). Muc-1 expression is reduced during the receptive phase in the rat uterus. Biol. Reprod. 58, 1503–1507.
CrossRef | CAS | PubMed |

Enders, A. C., and Liu, I. K. M. (1991). Trophoblast–uterine interactions during equine chorionic girdle cell, maturation, migration and transformation. Am. J. Anat. 192, 366–381.
CrossRef | CAS | PubMed |

Gastal, M. O., Gastal, E. L., Kot, K., and Ginther, O. J. (1996). Factors related to the time of fixation of the conceptus in mares. Theriogenology 46, 1171–1180.
CrossRef | CAS | PubMed |

Gillies, L. K., Waelchli, R. O., Ruddock, W. D. J., Betteridge, K. J., and LaMarre, J. (1999). Patterns of MUC1 expression in the equine endometrium and trophoblast during early pregnancy. Theriogenology 51, 225.

Ginther, O. J. (1983a). Mobility of the early equine conceptus. Theriogenology 19, 613–623.
CrossRef | CAS | PubMed |

Ginther, O. J. (1983b). Fixation and orientation of the early equine conceptus. Theriogenology 19, 613–623.
CrossRef | CAS | PubMed |

Hewetson, A., and Chilton, B. S. (1997). Molecular cloning and hormone-dependent expression of rabbit MUC1 in the cervix and uterus. Biol. Reprod. 57, 468–477.
CrossRef | CAS | PubMed |

Hey, N. A., and Aplin, J. D. (1996). Sialyl-Lewis-X and Sialyl-Lewis are associated with MUC1 in human endometrium. Glycoconj. J. 13, 769–779.
CrossRef | CAS | PubMed |

Hey, N. A., Meseguer, M., Simon, C., Smorodinsky, N. I., Wreschner, D. H., Ortiz, M. E., and Aplin, J. D. (2003). Transmembrane and truncated (SEC) isoforms of MUC1 in the human endometrium and fallopian tube. Reprod. Biol. Endocrinol. 1, 2.
CrossRef | PubMed |

Hild-Petito, S., Fazleabas, A. T., Julian, J., and Carson, D. D. (1996). Mucin (Muc-1) expression is differentially regulated in uterine luminal and glandular epithelia of the baboon (Papio anubis). Biol. Reprod. 54, 939–947.
CrossRef | CAS | PubMed |

Hilkens, J., Ligtenberg, M. J. L., Vos, H. L., and Litvinov, S. V. (1992). Cell membrane-associated mucins and their adhesion-modulating property. Trends Biochem. Sci. 17, 359–363.
CrossRef | CAS | PubMed |

Hoffman, L. H., Olsen, G. E., Carson, D. D., and Chilton, B. S. (1998). Progesterone and implanting blastocysts regulate MUC1 expression in rabbit uterine epithelium. Endocrinology 139, 266–271.
CrossRef | CAS | PubMed |

Johnson, G. A., Bazer, F. W., Jaeger, L. A., Ka, H., Garlow, J. E., Pfarrer, C., Spencer, T. E., and Burghardt, R. C. (2001). Muc-1, integrin and oesteopontin expression during the implantation cascade in sheep. Biol. Reprod. 65, 820–828.
CrossRef | CAS | PubMed |

Jones, C. J. P., Wooding, F. B. P., Dantzer, V., Leiser, R., and Stoddart, R. W. (1999). A lectin-binding analysis of glycosylation patterns during development of the equine placenta. Placenta 20, 45–57.
CrossRef | CAS |

Lasky, L. A. (1992). Selectins: interpreters of cell-specific carbohydrate information during inflammation. Science 258, 964–969.
CrossRef | CAS | PubMed |

Levitin, F., Stern, O., Weiss, M., Gil-Henn, C., Ziv, R., Prokocimer, Z., Smorodinsky, N. I., Rubinstein, D. B., and Wreschner, D. H. (2005). The MUC1 SEA module is a self-cleaving domain. J. Biol. Chem. 280, 33 374–33 386.
CrossRef | CAS |

Mahanta, S., Fessler, S. P., Park, J., and Bamdad, C. (2008). A minimal fragment of MUC1 mediates growth of cancer cells. PLoS ONE 3, e2054.
CrossRef | PubMed |

Meadows, S. (1996) The development and invasion of the chorionic girdle in equids. PhD thesis. University of Cambridge.

Meadows, S., Allen, W. R., and Antczak, D. F. (1995). Factors affecting the maturation and invasiveness in vitro of chorionic girdle cells of different genotypes. Biol. Reprod. Mono. Series 1, 171–180.

Meseguer, M., Pellicer, A., and Simón, C. (1998). MUC1 and endometrial receptivity. Mol. Hum. Reprod. 4, 1089–1098.
CrossRef | CAS | PubMed |

Meseguer, M., Aplin, J. D., Cabellero-Campo, P., O’Connor, J. E., Martín, J. C., Remohí, J., Pellicer, A., and Simón, C. (2001). Human endometrial mucin MUC1 is up-regulated by progesterone and down-regulated in vitro by the human blastocyst. Biol. Reprod. 64, 590–601.
CrossRef | CAS | PubMed |

Obermair, A., Schmid, B. C., Packer, L. M., Leodolter, S., Birner, P., Ward, B. G., Crandon, A. J., McGuckin, M. A., and Zeillinger, R. (2002). Expression of MUC1 splice variants in benign and malignant ovarian tumours. Int. J. Cancer 100, 166–171.
CrossRef | CAS | PubMed |

Olson, G.E., Winfrey, V. P., Matrisian, P. E., NagDas, S. K., and Hoffman, L. H. (1998). Blastocyst-dependent upregulation of metalloproteinase/disintegrin MDC9 expression in the rabbit endometrium. Cell Tissue Res. 293, 489–498.
CrossRef | CAS | PubMed |

Oriol, J. G., Betteridge, K. J., Clarke, A. J., and Sharom, F. J. (1993a). Mucin-like glycoproteins in the equine embryonic capsule. Mol. Reprod. Dev. 34, 255–265.
CrossRef | CAS | PubMed |

Oriol, J. G., Sharom, F. J., and Betteridge, K. J. (1993b). Developmentally-regulated changes in the glycoproteins of the equine embryonic capsule. J. Reprod. Fertil. 99, 653–664.
CrossRef | CAS | PubMed |

Pemberton, L., Taylor-Papadimitriou, J., and Gendler, S. J. (1992). Antibodies to the cytoplasmic domain of the MUC1 mucin show conservations throughout mammals. Biochem. Biophys. Res. Commun. 185, 167–175.
CrossRef | CAS | PubMed |

Ren, Q., Guan, S., Fu, J., and Wang, A. (2010). Temporal and spatial expression of MUC1 during implantation in sows. Int. J. Mol. Sci. 11, 2322–2335.
CrossRef | CAS | PubMed |

Samuel, C. A., Allen, W. R., and Steven, D. H. (1974). Studies on the development of the equine placenta. I. Development of the microcotyledons. J. Reprod. Fertil. 41, 441–445.
CrossRef | CAS | PubMed |

Shalom-Barak, T., Nicholas, J. M., Wang, Y., Zhang, X., Ong, E. S., Young, T. H., Gendler, S. J., Evans, R. M., and Barak, Y. (2004). Peroxisome proliferator-activated receptor gamma controls Muc1 transcription in trophoblasts. Mol. Cell. Biol. 24, 10661–10669.
CrossRef | CAS | PubMed |

Shyu, M.-K., Lin, M.-C., Fu, Y.-R., Shih, J.-C., Lee, C.-N., Chen, H.-Y., Huang, J., Huang, M. C., and Hsieh, F. J. (2008). MUC1 expression is increased during human placental development and suppresses trophoblast-like cell invasion in vitro. Biol. Reprod. 79, 233–239.
CrossRef | CAS | PubMed |

Surveyor, G. A., Gendler, S. J., Pemberton, L., Das, S. K., Chakraborty, I., Julian, J., Pimental, R. A., Wegner, C. C., Dey, S. K., and Carson, D. D. (1995). Expression and steroid hormonal control of Muc1 in the mouse uterus. Endocrinology 136, 3639–3647.
CrossRef | CAS | PubMed |

Thirkill, T. L., Cao, T., Stout, M., Blankenship, T. N., Barakat, A., and Douglas, G. S. (2007). MUC1 is involved in trophoblast transendothelial migration. Biochim. Biophys. Acta 1773, 1007–1014.
CrossRef | CAS | PubMed |

van Niekerk, C. H. (1965). The early diagnosis of pregnancy, the development of the foetal membranes and nidation in the mare. J. S. Afr. Vet. Med. Assoc. 36, 484–488.

Zrihan-Licht, S., Baruch, A., Elroy-Stein, O., Keydar, I., and Wreschner, D. H. (1994a). Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules. FEBS Lett. 356, 130–136.
CrossRef | CAS | PubMed |

Zrihan-Licht, S., Vos, H. L., Baruch, A., Elroy-Stein, O., Sagiv, D., Keydar, I., Hilkens, J., and Wreschner, D. H. (1994b). Characterization and molecular cloning of a novel MUC1 protein, devoid of tandem repeats, expressed in human breast cancer tissue. Eur. J. Biochem. 224, 787–795.
CrossRef | CAS | PubMed |


   
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