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Article << Previous     |     Next >>   Contents Vol 32(4)

Genetic variation among Stipa grandis P. Smirn populations with different durations of fencing in the Inner Mongolian Steppe

Jian-bo Wu A , Yu-bao Gao A B , Xiao-ying Bao A , Hui Gao A , Mei-qing Jia A , Jie Li A and Nian-xi Zhao A

A College of Life Science, Nankai University, Tianjin 300071, P.R. China.
B Corresponding author. Email: ybgao@mail.nankai.edu.cn

The Rangeland Journal 32(4) 427-434 http://dx.doi.org/10.1071/RJ09038
Submitted: 1 July 2009  Accepted: 12 October 2010   Published: 26 November 2010


 
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Abstract

The genetic structure of a population should be carefully considered in ecological restoration because it may play a critical role in maintaining the persistence of a restored ecosystem. In the present study, we examined genetic diversity and genetic structure of Stipa grandis P. Smirn populations from fenced and grazed plots using amplified fragment length polymorphism markers. Molecular genetic variation showed that the genetic diversity of the fenced populations was greater compared with the overgrazed population. There was a significant variation among the populations (Fst = 0.3689, P < 0.001) by AMOVA analysis, and the gene flow was 0.4039 among the populations. The results from a comparison of limited morphological characteristics and from an unweighted pair group method with arithmetic mean cluster analysis and non-metric multi-dimensional scaling analysis suggested that genetic differentiation had occurred between the fenced populations and the grazed populations. The largest genetic diversity was in the moderately grazed population, which might be related to higher population density and greater sexual reproduction due to less disturbances in the plots. The genetic diversity of the long-term (24 years) fenced population was similar to that of a short-term fenced population (fenced for 11 years). These results suggested that the genetic diversity in the overgrazed population might be increased to some extent through fencing, but this effect did not occur beyond 11 years.

Additional keywords:amplified fragment length polymorphism, genetic diversity, population restoration, Stipa grandis.


References

An, Y., Li, B., Yang, C., Yan, Z. J., and Han, G. D. (2002). Influence of grazing rate on population structure of Stipa grandis. Acta Phytoecologica Sinica 26, 163–169.

Bassam, B. J., Caetano-Anollés, G., and Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196, 80–83.
CrossRef | CAS | PubMed |

Chaneton, E. J., and Lavado, R. S. (1996). Soil nutrients and salinity after long-term grazing exclusion in a flooding Pampa grassland. Journal of Range Management 49, 182–187.
CrossRef |

Cheng, J. M., and Zou, H. Y. (1998). Effects of protective growing cutting and grazing on the vegetation of grassland. Research of Soil and Water Conservation 5, 36–54.

Crutsinger, G. M., Collins, M. D., Fordyce, J. A., Gompert, Z., Nice, C. C., and Sanders, N. J. (2006). Plant genotypic diversity predicts community structure and governs an ecosystem process. Science 313, 966–968.
CrossRef | CAS | PubMed |

Dolan, R. W., Marr, D. L., and Schnabel, A. (2008). Capturing genetic variation during ecological restorations: an example from Kankakee sands in Indiana. Restoration Ecology 16, 386–396.
CrossRef |

Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics 11, 1–42.
CrossRef |

Excoffier, L., Smouse, P. E., and Quattro, J. M. (1992). Analysis of molecular variance inferred form metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479–491.
| CAS | PubMed |

Fenster, C. B., and Dudash, M. R. (1994). Genetic considerations for plant population restoration and conservation. In: ‘Restoration of Endangered Species: Conceptual Issues, Planning and Implementation’. (Eds M. L. Bowles and C. Whelan.) pp. 34–62. (Cambridge University Press: Cambridge.)

Fırıncıoğlu, H. K., Seefeldt, S. S., and Şahin, B. (2007). The effects of long-term grazing exclosures on range plants in the central Anatolian region of Turkey. Environmental Management 39, 326–337.
| PubMed |

Fu, Y. B., Thompson, D., Willms, W., and Mackay, M. (2005). Long-term grazing effects on genetic variability in mountain rough fescue. Rangeland Ecology and Management 58, 637–642.
CrossRef |

Gustafson, D. J., Gibson, D. J., and Nickrent, D. L. (2002). Genetic diversity and competitive abilities of Dalea purpurea (Fabaceae) from remnant and stored grasslands. International Journal of Plant Sciences 163, 979–990.
CrossRef | CAS |

Gustafson, D. J., Gibson, D. J., and Nickrent, D. L. (2004). Conservation genetics of two co-dominant grass species in an endangered grassland ecosystem. Journal of Applied Ecology 41, 389–397.
CrossRef | CAS |

Huff, D. R., Quinn, J. A., Higgins, B., and Palazzo, A. J. (1998). Random amplified polymorphic DNA (RAPD) variation among native little bluestem [Schizachyrium scoparium (Michx.) Nash] populations from sites of high and low fertility in forest and grassland biomes. Molecular Ecology 7, 1591–1597.
CrossRef | CAS |

Ingvarsson, P. K. (2002). A meta-population perspective on genetic diversity and differentiation in partially self-fertilizing plants. Evolution 56, 2368–2373.
| PubMed |

Knapp, E. E., and Dyer, A. R. (1998). When do genetic considerations require special approaches to ecological restoration? In: ‘Conservation Biology for the Coming Decade’. 2nd edn. (Eds P. L. Fiedler and P. M. Kareiva.) pp. 345–363. (Chapman and Hall Inc.: New York.)

Krauss, S. L., and Koch, J. M. (2004). Rapid genetic delineation of provenance for plant community restoration. Journal of Applied Ecology 41, 1162–1173.
CrossRef |

Lavado, R. S., Sierra, J. O., and Hashimoto, P. N. (1996). Impact of grazing on soil nutrients in a Pampean grassland. Journal of Range Management 49, 452–457.
CrossRef |

Lesica, P., and Allendorf, F. W. (1999). Ecological genetics and the restoration of plant communities: mix or match. Restoration Ecology 7, 42–50.
CrossRef |

Li, Y. P., and Chen, S. H. (1996). The eco-biological characteristics of reproduction of Stipa grandis. Journal of Inner Mongolian institute of Agriculture and Animal Husbandry 17, 7–13.

Liston, A., Wilson, B. L., Robinson, W. A., Doescher, P. S., Harris, N. R., and Svejcar, T. (2003). The relative importance of sexual reproduction versus clonal spread in an arid-land bunchgrass. Oecologia 137, 216–225.
CrossRef | CAS | PubMed |

Liu, M. L., Taogetao, B. Y., Yang, C., and Zhang, X. S. (2007). Effect of mowing systems on composition of Stipa grandis community in Inner Mongolian steppe. Journal of Beijing Normal University (Natural Science) 43, 83–87.

Liu, M. H., Chen, X. Y., Zhang, X., and Shen, D. W. (2008). A population genetic evaluation of ecological restoration with the case study on Cyclobalanopsis myrsinaefolia (Fagaceae). Plant Ecology 197, 31–41.
CrossRef |

Martin, L. M., Moloney, K. A., and Wilsey, B. J. (2005). An assessment of grassland restoration success using species diversity components. Journal of Applied Ecology 42, 327–336.
CrossRef |

Matlaga, D., and Karoly, K. (2004). Long-term grazing effects on genetic variation in Idaho fescue. Journal of Range Management 57, 275–279.
CrossRef |

Montalvo, A. M., Williams, S. L., Rice, K. J., Buchmann, S. L., Cory, C., Handel, S. N., Nabhan, G. P., Primack, R. P., and Robichaux, R. H. (1997). Restoration biology: a population perspective. Restoration Ecology 5, 277–290.
CrossRef |

Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70, 3321–3323.
CrossRef | CAS | PubMed |

Nybom, H. (2004). Comparison of different nuclear DNA markers for estimating intra-specific genetic diversity in plants. Molecular Ecology 13, 1143–1155.
CrossRef | CAS | PubMed |

Ramp, J. M., Collinge, S. K., and Ranker, T. A. (2006). Restoration genetics of the vernal pool endemic Lasthenia conjugens (Asteraceae). Conservation Genetics 7, 631–649.
CrossRef | CAS |

Reisch, C., Poschlod, P., and Wingender, R. (2003). Genetic differentiation among populations of Sesleria albicans Kit. Ex Schultes (Poaceae) from ecologically different habitats in central Europe. Heredity 91, 519–527.
CrossRef | CAS | PubMed |

Reusch, T. B. H., Ehlers, A., Hammerli, A., and Worm, B. (2005). Ecosystem recovery after climatic extremes enhanced by genotypic diversity. Proceedings of the National Academy of Sciences of the United States of America 102, 2826–2831.
CrossRef | CAS | PubMed |

Rohlf, F. J. (1994). ‘NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System. version 1.80.’ (Exeter Software: New York.)

Seliskar, D. M., Gallagher, J. L., Burdick, D. M., and Mutz, L. A. (2002). The regulation of ecosystem functions by ecotypic variation in the dominant plant: a Spartina alterniflora salt-marsh case study. Journal of Ecology 90, 1–11.
CrossRef |

Shan, D. M., Zhao, L., Han, B., and Han, G. D. (2006). Genetic diversity of Stipa grandis under different grazing pressures. Acta Ecologica Sinica 26, 3175–3183.
CrossRef | CAS |

Smulders, M. J. M., van der Schoot, J., Geerts, R. H. E. M., Antonisse-de Jong, A. G., Korevaar, H., van der Werf, A., and Vosman, B. (2000). Genetic diversity and the reintroduction of meadow species. Plant Biology 2, 447–454.
CrossRef |

Society for Ecological Restoration International Science and Policy Working Group (2004). ‘The SER International Primer on Ecological Restoration.’ (Society for Ecological Restoration International: Tucson, AZ.) Available at: www.ser.org (accessed 4 April 2006)

SPSS (1999) ‘SPSS 10.0 for Windows.’ (SPSS Inc.: Chicago, IL) Available at: www.spss.com

Sternberg, M., Gutman, M., Perevolotsky, A., Ungar, E. D., and Kigel, J. (2000). Vegetation response to grazing management in a Mediterranean herbaceous community: a functional group approach. Journal of Applied Ecology 37, 224–237.
CrossRef |

The Vegetation of Inner Mongolia (1985). ‘Integrated Survey Team to Inner Mongolia-Ningxia.’ (Eds The Chinese Academy of Sciences.) pp. 495–516. (Science Press: Beijing.)

Tomás, M. A., Carrera, A. D., and Poverene, M. (2000). Is there any genetic differentiation among populations of Piptochaetium napostaense (Speg.) Hack (Poaceae) with different grazing histories? Plant Ecology 147, 227–235.
CrossRef |

Travis, S. E., Proffitt, C. E., Lowenfeld, R. C., and Mitchell, T. W. (2002). A comparative assessment of genetic diversity among differently aged populations of Spartina alterniflora on restored versus natural wetlands. Restoration Ecology 10, 37–42.
CrossRef |

Urbanska, K. M., Webb, N., and Edwards, P. (1997). ‘Restoration Ecology and Sustainable Development.’ (Cambridge University Press: Cambridge.)

Vellend, M., and Waterway, M. J. (1999). Geographic patterns in the genetic diversity of northern sedge, Carex rariflora. Canadian Journal of Botany 77, 269–278.
CrossRef |

Volis, S., Yakubov, B., Shulgina, I., Ward, D., Zur, V., and Mendlinger, S. (2001). Tests for adaptive RAPD variation in population genetic structure of wild barley, Hordeum spontaneum Koch. Biological Journal of the Linnaean Society 74, 289–303.
CrossRef |

Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Friters, A., Pot, J., Paleman, J., Kuiper, M., and Zabeau, M. (1995). AFLP: a new technique for DNA finger printing. Nucleic Acids Research 23, 4407–4414.
CrossRef | CAS | PubMed |

Wang, J., Yang, C., Yin, J., Wang, T. J., and Liu, P. T. (2004). Changes of the genetic diversity of Artemisia frigida population under the disturbance of grazing. Acta Ecologica Sinica 24, 2455–2471.

Wang, W., Liu, Z. L., Hao, D. Y., and Liang, C. Z. (1996). Research on the restoring succession of the degenerated grassland in Inner Mongolia I. Basic characteristics and driving force for restoration of the degenerated grassland. Acta Phytoecologica Sinica 20, 449–459.

Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.
CrossRef | CAS | PubMed |

Wright, S. (1931). Evolution in Mendelian populations. Genetics 16, 97–159.
| CAS | PubMed |

Wright, S. (1951). The genetical structure of populations. Annals of Eugenics 15, 323–354.

Wu, J. B., Chen, C. B., Bao, X. Y., Song, W. Q., Zhao, N. X., and Gao, Y. B. (2009). Chromosome numbers and karyotypes of Stipa baicalensis, Stipa grandis and Stipa krylovii in Inner-Mongolia Steppe. Bulletin of Botanical Research 29, 534–538.

Yeh, F. C., Yang, R. C., and Boyle, T. (1999). ‘POPGENE version 1.32. Microsoft Windows-based Software for Population Genetic Analysis: A Quick User’s Guide.’ (University of Alberta, Centre for International Forestry Research: Alberta.)

Zhang, H. M., Zhao, M. L., Li, Q. F., and Han, B. (2003). Morphological variations of Stipa grandis under grazing stress. Grasslands of China 25, 13–17.

Zhao, N. X., Gao, Y. B., Wang, J. L., Ren, A. Z., and Xu, H. (2006). RAPD diversity of Stipa grandis populations and its relationship with some ecological factors. Acta Ecologica Sinica 26, 1312–1319.
CrossRef | CAS |

Zhao, N. X., Gao, Y. B., Wang, J. L., and Ren, A. Z. (2008). Population structure and genetic diversity of Stipa grandis P. Smirn, a dominant species in the typical steppe of northern China. Biochemical Systematics and Ecology 36, 1–10.
CrossRef |

Zhao, M. L., Willms, W. D., Han, B., and Laroche, A. (2005). Effect of heavy grazing pressure on the random amplified polymorphic DNA marker diversity of mountain rough fescue (Festuca campestris Rybd.) in south western Alberta. Canadian Journal of Plant Science 85, 623–629.


   
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