Register      Login
Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Enhancement of physicochemical, microbiological and sensory properties of dry-cured loin by using processed sulfur-fed pigs

Ji-Han Kim A , Go-Eun Hong A , Chang-Won Pyun A , Woojoon Park A and Chi-Ho Lee A B
+ Author Affiliations
- Author Affiliations

A Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 143-701, Republic of Korea.

B Corresponding author. Email: leech@konkuk.ac.kr

Animal Production Science 56(11) 1936-1945 https://doi.org/10.1071/AN14680
Submitted: 9 July 2014  Accepted: 14 May 2015   Published: 12 October 2015

Abstract

The present study showed that processed sulfur supplementation and processing condition affected changes in physicochemical, microbial and sensory properties of dry-cured loin. The pigs were divided into two groups at the fattening phase: non-sulfur-fed pigs (NSFP) and 0.3% processed sulfur-fed pigs (SFP) in fattening phase. The crude fat content of SFP was lower than that of NSFP in raw meat and dry-cured loin. Lipid and pigment oxidation were suppressed in SFP compared with NSFP during whole process. Lightness of SFP was significantly higher than that of NSFP during storage. SFP showed higher colour stability than did NSFP during storage. Moreover, microbial spoilage of dry-cured loin was greatly prevented in SFP compared with NSFP. Free amino acids related to taste in SFP were significantly higher than those in NSFP. Polyunsaturated fatty acid of dry-cured loin from SFP group was higher than that of NSFP, while saturated fatty acid of NSFP was significantly higher than that of SFP. SFP had higher preference and flavour scores than did NSFP in a sensory test. Feeding processed sulfur improved the meat-quality characteristics and extended the shelf-life of meat products.

Additional keywords: fatty acid composition, lipid oxidation, meat quality, nutrient quality.


References

Analytical Methods of Korean Food Standards Codex (2015a) Amino acid, 9-1.1.3.3.

Analytical Methods of Korean Food Standards Codex (2015b) Lipid analysis with acid decomposition, 9-1.1.5.4.

Anonymous (1994) Report on health and social subjects. No 46. Nutritional aspects of cardiovascular disease. Department of Health, Her Majesty’s Stationery Office, London.

AOAC (1995) ‘Official methods of analysis (16th edn).’ (Association of Official Analytical Chemists: Washington, DC)

Baka AM, Papavergou EJ, Pragalaki T, Bloukas JG, Kotzekidou P (2011) Effect of selected autochthonous starter cultures on processing and quality characteristics of Greek fermented sausages. LWT – Food Science and Technology (Campinas.) 44, 54–61.

Belalcázar AD, Ball JG, Frost LM, Valentovic MA, Wilkinson JT (2013) Transsulfuration is a significant source of sulphur for glutathione production in human mammary epithelial cells. ISRN Biochemistry 637897

Benedini R, Parolari G, Toscani T, Virgili R (2012) Sensory and texture properties of Italian typical dry-cured hams as related to maturation time and salt content. Meat Science 90, 431–437.
Sensory and texture properties of Italian typical dry-cured hams as related to maturation time and salt content.Crossref | GoogleScholarGoogle Scholar | 21955982PubMed |

Campo MM, Nute GR, Hughes SI, Enser M, Wood JD, Richardson RI (2006) Flavour perception of oxidation in beef. Meat Science 72, 303–311.
Flavour perception of oxidation in beef.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtFOgsLjO&md5=ea44898435cc50141340e6436ed2c9f2CAS | 22061558PubMed |

Careri M, Mangia A, Barbieri G, Bolzoni L, Virgili R, Parolari G (1993) Sensory property relationships to chemical data of Italian type dry cured ham. Journal of Food Science 58, 968–972.
Sensory property relationships to chemical data of Italian type dry cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXivFKquw%3D%3D&md5=f01b0340fee5e80be703cfe72eb8dddaCAS |

Chizzolini R, Rosa P, Novelli E (1993) Biochemical and microbiological events of parma ham production technology. Microbiologia SEM 9, 26–34.

Cho JH, Min BJ, Kwon OS, Shon KS, Jin YG, Kim HJ, Kim IH (2005) Effects of MSM supplementation on growth performance and digestibility of CA and N in pigs. Journal of the Korean Society of Food Science and Nutrition 34, 361–365.
Effects of MSM supplementation on growth performance and digestibility of CA and N in pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjvVynur4%3D&md5=c4c0878bb5c1a7815cb72dba0e9c2e0bCAS |

Clariana M, Guerrero L, Sarraga C, Garcia-Regueiro JA (2012) Effects of high pressure application (400 and 900 MPa) and refrigerated storage time on the oxidative stability of sliced skin vacuum packed dry-cured ham. Meat Science 90, 323–329.
Effects of high pressure application (400 and 900 MPa) and refrigerated storage time on the oxidative stability of sliced skin vacuum packed dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVWkt77M&md5=a66ff8e95a97d6c22d078901bd6e15a0CAS | 21856090PubMed |

Colussi G, Catena C, Dialti V, Mos L, Sechi AL (2014) Effects of the consumption of fish meals on the carotid intimamedia thickness in patients with hypertension: a prospective study. Journal of Atherosclerosis and Thrombosis 21, 941–956.
Effects of the consumption of fish meals on the carotid intimamedia thickness in patients with hypertension: a prospective study.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXitVyisb7E&md5=f79010f182fa49f8e293ada4937e0c33CAS | 24727682PubMed |

Egan AF (1983) Lactic acid bacteria of meat and meat products. Antonie van Leeuwenhoek 49, 327–336.
Lactic acid bacteria of meat and meat products.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaL2c%2Fit1Cltw%3D%3D&md5=62973d22a2de007b26026d30babc6844CAS | 6354082PubMed |

Enser M Scollan N Gulati S Richardson I Nute G Wood J 2001 The effects of ruminally protected dietary lipid on the lipid composition and quality of beef muscle. Proceedings of the 47th International Congress of Meat Science and Technology 1 12 13

Faustman C, Sun Q, Mancini R, Suman SP (2010) Myoglobin and lipid oxidation interactions: mechanistic bases and control. Meat Science 86, 86–94.
Myoglobin and lipid oxidation interactions: mechanistic bases and control.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXos1Cntrk%3D&md5=44d859e48f52821ab59de1f255b244dfCAS | 20554121PubMed |

Franco D, Gonzalez L, Bispo E, Latorre A, Moreno T, Sineiro J, Sanchez M, Nunez MJ (2012) Effects of calf diet, antioxidants, packaging type and storage time on beef steak storage. Meat Science 90, 871–880.

Fuentes V, Utrera M, Estevez M, Ventanas J, Ventanas S (2014a) Impact of high pressure treatment and intramuscular fat content on colour changes and protein and lipid oxidation in sliced and vacuum-packaged Iberian dry-cured ham. Meat Science 97, 468–474.
Impact of high pressure treatment and intramuscular fat content on colour changes and protein and lipid oxidation in sliced and vacuum-packaged Iberian dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXptVClt78%3D&md5=f9bde3667bc7c55a3fbe1ffaf4609d13CAS | 24769145PubMed |

Fuentes V, Estevez M, Ventanas J, Ventanas S (2014b) Impact of lipid content and composition on lipid oxidation and protein carbonylation in experimental fermented sausages. Food Chemistry 147, 70–77.
Impact of lipid content and composition on lipid oxidation and protein carbonylation in experimental fermented sausages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsleqsrvE&md5=8f6651731505c152bc150488a892c592CAS | 24206687PubMed |

Georgantelis D, Ambrosiadis I, Katikou P, Blekas G, Georgakis SA (2007) Effect of rosemary extract, chitosan and α-tocopherol on microbiology parameters and lipid oxidation of fresh pork sausages stored at 4°C. Meat Science 76, 172–181.
Effect of rosemary extract, chitosan and α-tocopherol on microbiology parameters and lipid oxidation of fresh pork sausages stored at 4°C.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXitFOrtrc%3D&md5=e3548358d07104ef115b9f571da4f4c9CAS | 22064204PubMed |

Guo Z, Miura K, Turin TC, Hozawa A, Okuda N, Okamura T, Sitoh S, Sakata K, Nakagawa H, Okayama A, Yoshita K, Kadowaki T, Choudhury SR, Nakamura Y, Rodriguez BL, Curb DJ, Elliott P, Stamler J, Ueshima H (2010) Relationship of the polyunsaturated to saturated fatty acid ratio to cardiovascular risk factors and metabolic syndrome in Japancese: the INTERLIPID study. Journal of Atherosclerosis and Thrombosis 17, 777–784.
Relationship of the polyunsaturated to saturated fatty acid ratio to cardiovascular risk factors and metabolic syndrome in Japancese: the INTERLIPID study.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhvVers7Y%3D&md5=5adc7242a62d0f51c172dbd5e9067145CAS | 20351467PubMed |

Hajeb P, Jinap S (2015) Umami taste components and their sources in Asian foods. Critical Reviews in Food Science and Nutrition 55, 778–791.
Umami taste components and their sources in Asian foods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsV2ksrnJ&md5=90033456f3dd89abcfca54688c563e95CAS | 24915349PubMed |

Hiroaki O (2006) Functions of sulfur-containing amino acids in lipid metabolism. The Journal of Nutrition 136, 1666S–1669S.

Ismail HA, Lee EJ, Ko KY, Ahn DU (2009) Fat content influences the color, lipid oxidation, and volatiles of irradiated ground beef. Journal of Food Science 74, 32–40.

Jang HD, Yoo JS, Chae SJ, Park SL, Kim IC, Park JC, Jung HJ, Kim YH, Kim IH, Seok HB (2006) Effects of dietary of methyl sulfonyl methane on growth performance and meat quality characteristics in growing-finishing pigs. Korean Journal of International Agriculture 18, 116–120.

Kim GS, Jeong YR, Hong SH, Lee NJ, Yu JH, Jeong JH, Kang JK (2006) The comparison of the toxicity of processed sulfur with non-processed sulfur in Sprague-dawley rats. Journal of Veterinary Medicine and Biotechnology 7, 183–190.

Kim JH, Lee HR, Pyun CW, Kim SK, Lee CH (2014) Changes in physicochemical, microbiological and sensory properties of dry-cured ham in processed sulfur-fed pigs. Journal of Food Processing and Preservation
Changes in physicochemical, microbiological and sensory properties of dry-cured ham in processed sulfur-fed pigs.Crossref | GoogleScholarGoogle Scholar |

Kim JH, Noh HY, Kim GH, Hong GE, Kim SK, Lee CH (2015) Effect of dietary supplementation with processed sulphur on meat quality and oxidative stability in Longissimus dorsi of pigs. Korean Journal for Food Science of Animal Resources 35, 330–338.

Komarnisky LA, Christopherson RJ, Basu TK (2003) Sulfur: Its clinical and toxicologic aspects. Nutrition (Burbank, Los Angeles County, Calif.) 19, 54–61.
Sulfur: Its clinical and toxicologic aspects.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpvVahs7k%3D&md5=580ca9197cb10b5a99e010906a031d5cCAS |

Lee JI, Min HK, Lee JW, Jeong JD, Ha YJ, Kwack SC, Park JS (2009) Changes in the quality of loin from pigs supplemented with dietary methyl sulfonyl methane during cold storage. Korean Journal of Food Science and Animal Resources 29, 229–237.

Lorenzo JM, Garcia Fontan MC, Cachaldora A, Franco I, Carballo J (2010) Study of the lactic acid bacteria throughout the manufacture of dry cured lacon (a spanish traditional meat product). Effect of some additives. Food Microbiology 27, 229–235.
Study of the lactic acid bacteria throughout the manufacture of dry cured lacon (a spanish traditional meat product). Effect of some additives.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlGhu78%3D&md5=602d491925b016d305e32b925fad8626CAS | 20141940PubMed |

Luo Z, Gasasira V, Huang Y, Liu D, Yang X, Jiang S, Hu W (2013) Effect of Lactobacillus salivarius H strain isolated from Chinese dry-cured ham on the color stability of fresh pork. Food Science and Human Wellness 2, 139–145.
Effect of Lactobacillus salivarius H strain isolated from Chinese dry-cured ham on the color stability of fresh pork.Crossref | GoogleScholarGoogle Scholar |

Maurice R, Didier A (1980) Culture method to study fungal growth in solid fermentation. European Journal of Applied Microbiology and Biotechnology 9, 199–209.

McBean GJ (2012) The transsulfuration pathway: a source of cysteine for glutathione in astrocytes. Amino Acids 42, 199–205.
The transsulfuration pathway: a source of cysteine for glutathione in astrocytes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xit1yhtw%3D%3D&md5=715f39be64482b967f8367b770866d5dCAS | 21369939PubMed |

Ming T, Li JJ, Huo P, Wei Y, Chen X (2014) Analysis of free amino acids in russula griseocarnosa harvested at different stages of maturity using iTRAQ–LC–MS/MS. Food Analyrical Methods 7, 1816–1823.
Analysis of free amino acids in russula griseocarnosa harvested at different stages of maturity using iTRAQ–LC–MS/MS.Crossref | GoogleScholarGoogle Scholar |

Mosharov E, Cranford MR, Banerjee R (2000) The quantitatively important relationship between homocysteine metabolism and glutathione synthesis by the transsulfuration pathway and its regulation by redox changes. Biochemistry 39, 13005–13011.
The quantitatively important relationship between homocysteine metabolism and glutathione synthesis by the transsulfuration pathway and its regulation by redox changes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXms1OlsLw%3D&md5=0289e1fb2002bf940432d1e6584638bbCAS | 11041866PubMed |

Nishimura T, Kato H (1988) Taste of free amnino acids and peptides. Food Reviews International 4, 175–194.
Taste of free amnino acids and peptides.Crossref | GoogleScholarGoogle Scholar |

Offer G (1989) The structural basis of water-holding, appearance and toughness of meat and meat products. Food Microstructure 8, 151–170.

Papanagiotou P, Tzimitra-Kalogianni I, Melfou K (2013) Consumer’s expected quality and intention to purchase high quality pork meat. Meat Science 93, 449–454.
Consumer’s expected quality and intention to purchase high quality pork meat.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3s3lt1ajsA%3D%3D&md5=912a0ec7278d35c3d00e966b17f95d8cCAS | 23273449PubMed |

Park JH, Ryu MS, Lee YE, Song GS, Ryu KS (2003) A comparision of fattening performance, physic-chemical properties of breast meat, vaccine titers in cross bred meat type hybrid chicks fed sulfur. Korean Journal of Poultry Science 30, 211–217.

Pogge D, Hansen S (2012) Effect of vitamin C on performance and antioxidant capacity of cattle fed varying concentrations of dietary sulfur. Iowa University Animal Industry Report AS 658, ASL R2689.

Pogge DJ, Lonergan SM, Hansen SL (2014) Influence of supplemental vitamin C on postmortem protein degradation and fatty acid profiles of the longissimus thoracis of steers fed varying concentrations of dietary sulfur. Meat Science 96, 956–963.
Influence of supplemental vitamin C on postmortem protein degradation and fatty acid profiles of the longissimus thoracis of steers fed varying concentrations of dietary sulfur.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhvFelu7bM&md5=6466ce87d22ecce71a6d719fa113141fCAS | 24211555PubMed |

Reza T, Litha S, Jacek J (2012) Dynamic rheology and endothermic transitions of proteins recovered from chicken-meat processing by-products using isoelectric solubilization/precipitation and addition of TiO2. LWT – Food Science and Technology 46, 148–155.

Richter EL, Drewnoski ME, Hansen SL (2014) Effects of increased dietary sulfur on beef steer mineral status, performance, and meat fatty acid composition. Journal of Animal Science 90, 3945–3953.
Effects of increased dietary sulfur on beef steer mineral status, performance, and meat fatty acid composition.Crossref | GoogleScholarGoogle Scholar |

Ruiz J, Garcia C, Muriel E, Andres AI, Ventanas J (2002) Influence of sensory characteristics on the acceptability of dry-cured ham. Meat Science 61, 347–354.
Influence of sensory characteristics on the acceptability of dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3s%2Fhtl2lug%3D%3D&md5=0a833aeaf093a2fab597bccd14711975CAS | 22061062PubMed |

Sanchez-Zapata E, Zunino V, Perez AJA, Fernandez LJ (2013) Effect of tiger nut fibre addition on the quality and safety of a dry-cured pork sausage (‘chorizo’) during the dry-curing process. Meat Science 95, 562–568.
Effect of tiger nut fibre addition on the quality and safety of a dry-cured pork sausage (‘chorizo’) during the dry-curing process.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtFShsL7F&md5=03efa43b8b17188e1dda380c2fe050d0CAS | 23793111PubMed |

Simopoulos AP (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomedicine and Pharmacotherapy 56, 365–379.
The importance of the ratio of omega-6/omega-3 essential fatty acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XotVKrtbY%3D&md5=01efed77103d95718c33ee11f74ee684CAS | 12442909PubMed |

Song R, Chen C, Wang L, Johnston LJ, Kerr BJ, Weber TE, Shurson GC (2013) High sulfur content in corn dried distillers grains with soluble protects against oxidized lipids by increasing sulfur-containing antioxidants in nursery pigs. Journal of Animal Science 91, 2715–2728.
High sulfur content in corn dried distillers grains with soluble protects against oxidized lipids by increasing sulfur-containing antioxidants in nursery pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXpvFCrtr8%3D&md5=2ee4ed69d704d41f137f065528f0f226CAS | 23482577PubMed |

Soto E, Hoz L, Ordonez JA, Hierro E, Herranz B, Lopez-Bote C, Cambero MI (2008) Impact of feeding and rearing systems of Iberian pigs on volatile profile and sensory characteristics of dry-cured loin. Meat Science 79, 666–676.
Impact of feeding and rearing systems of Iberian pigs on volatile profile and sensory characteristics of dry-cured loin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXnsF2qt7g%3D&md5=a389ef76053f2877ba9ab02ab85f13cfCAS | 22063028PubMed |

Stollewerk K, Jofre A, Comaposada J, Arnau J, Garriga M (2012) The effect of NaCl- free processing and high pressure on the fate of Listeria monocytogenes and Salmonella on sliced smoked dry-cured ham. Meat Science 90, 472–477.
The effect of NaCl- free processing and high pressure on the fate of Listeria monocytogenes and Salmonella on sliced smoked dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVWkt7%2FI&md5=b9d618ca366de4ef79af5c00dbe2911dCAS | 22000500PubMed |

Stone H, Sidel JL (1993) ‘Sensory evaluation practices.’ (University of California: San Diego, CA)

Tobin BD, O’Sullivan MG, Hamill RM, Kerry JP (2013) The impact of salt and fat level variation on the physicochemical properties and sensory quality of pork breakfast sausages. Meat Science 93, 145–152.
The impact of salt and fat level variation on the physicochemical properties and sensory quality of pork breakfast sausages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhs12js77M&md5=39ee17c0a32732dc3c5780421c826476CAS | 23022579PubMed |

Toldrá F (1998) Proteolysis and lipolysis in flavour developlment of dry-cured meat products. Meat Science 49, S101–S110.
Proteolysis and lipolysis in flavour developlment of dry-cured meat products.Crossref | GoogleScholarGoogle Scholar |

Toldrá F (2006) The role of muscle enzymes in dry- cured meat products with different drying conditions. Trends in Food Science & Technology 17, 164–168.
The role of muscle enzymes in dry- cured meat products with different drying conditions.Crossref | GoogleScholarGoogle Scholar |

Toldrá F, Flores M (1998) The role of muscle proteases and lipases in flavor development during the processing of dry-cured ham. Critical Reviews in Food Science and Nutrition 38, 331–352.
The role of muscle proteases and lipases in flavor development during the processing of dry-cured ham.Crossref | GoogleScholarGoogle Scholar | 9626490PubMed |

Vaithiyanathan S, Naveena BM, Muthukumar M, Girish PS, Kondaiah N (2011) Effect of dipping in pomegranate (Punica granatum) fruit juice phenolic solution on the shelf life of chicken meat under refrigerated storage (4°C). Meat Science 88, 409–414.
Effect of dipping in pomegranate (Punica granatum) fruit juice phenolic solution on the shelf life of chicken meat under refrigerated storage (4°C).Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3M3psVWqsw%3D%3D&md5=7a67d2f2ed671e1b174751614561137fCAS | 21345604PubMed |

Virgili R, Schivazappa C (2002) Muscle traits for long matured dried meats. Meat Science 62, 331–343.
Muscle traits for long matured dried meats.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3MbnsValtA%3D%3D&md5=6d527751f92cba74682e8bc4f1dcffc0CAS | 22061609PubMed |

Winger RJ, Hagyard CJ (1994) Juiciness – its importance and some contributing factors. In ‘Quality attributes and their measurement in meat, poultry and fish products’. (Eds AM Pearson, TR Duston) pp. 94–124. (Blackie Academic & Professional: London)

Witte VC, Krause GF, Bailey ME (1970) A new extraction method for determining 2-thiobarbituric acid values of pork. Journal of Food Science 35, 582–585.

Zanardi E, Novelli E, Ghiretti GP, Chizzolini R (2000) Oxidative stability of lipids and cholesterol in salame Milano, coppa and parma ham: dietary supplementation with vitamic E and oleic acid. Meat Science 55, 169–175.
Oxidative stability of lipids and cholesterol in salame Milano, coppa and parma ham: dietary supplementation with vitamic E and oleic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1yrs7o%3D&md5=b56db8769c72a451009c74a84db2779fCAS | 22061082PubMed |