Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Polymorphisms of kappa-casein gene and their effects on milk production traits in Holstein, Jersey and Brown Swiss cattle*

Murad Gurses A E , Huseyin Yuce B , Ebru Onalan Etem C and Bahri Patir D
+ Author Affiliations
- Author Affiliations

A Balikesir University Faculty of Veterinary Medicine Department of Genetics, 10145 Balikesir, Turkey.

B Duzce University Faculty of Medicine Department of Medical Biology, 81000 Duzce, Turkey.

C Firat University Faculty of Medicine Department of Medical Biology, 23119 Elazig, Turkey.

D Firat University Faculty of Veterinary Medicine Department of Food Hygiene and Tecnology, 23119 Elazig, Turkey.

E Corresponding author. Email: mgurses@balikesir.edu.tr

Animal Production Science - https://doi.org/10.1071/AN15131
Submitted: 11 March 2015  Accepted: 1 October 2016   Published online: 2 December 2016

Abstract

The objectives of this study were to detect kappa-casein (κ-CN, CSN3, CASK) gene polymorphisms and to investigate the association between the κ-CN gene polymorphisms and milk yield and composition in cattle. For this purpose, a total of 365 blood and milk samples were collected from Holstein, Jersey and Brown Swiss cows. Polymorphisms of κ-CN gene were detected by the PCR and restriction fragment length polymorphism. A 776-bp fragment of exon IV was amplified by PCR and digested with HindIII and HaeIII restriction endonuclease to distinguish allele A, B and E. The analysis of associations between κ-CN genotypes and milk yield and composition were conducted with the use of the general linear model procedure. As a result, allele frequencies were detected as 0.736, 0.357, 0.308 for A allele, 0.186, 0.643, 0.692 for B allele in Holstein, Brown Swiss and Jersey breeds, respectively, and 0.079 for E allele in Holstein. This study also demonstrated the presence of E allele in Turkish Holstein. Although κ-CN genotypes were associated with protein and solids-not-fat content of milk in Hostein and Brown Swiss, they were associated with milk fat content in Jerseys (P < 0.05).

Additional keywords: Bovine CSN3, genetic polymorphism, milk yield and composition, PCR-RFLP.


References

Akyuz B, Agaoglu OK, Ertugrul O (2012) Genetic polymorphism of kappa-casein, growth hormone and prolactin genes in Turkish native cattle breeds. International Journal of Dairy Technology 65, 38–44.
Genetic polymorphism of kappa-casein, growth hormone and prolactin genes in Turkish native cattle breeds.CrossRef | 1:CAS:528:DC%2BC38Xis1aqu7c%3D&md5=58531c8f44cb6a84bfdfaae8412e1dacCAS |

Aleandri R, Buttazzoni LG, Schneider JC, Caroli A, Davoli R (1990) The effects of milk protein polymorphisms on milk components and cheese-producing ability. Journal of Dairy Science 73, 241–255.
The effects of milk protein polymorphisms on milk components and cheese-producing ability.CrossRef |

Alexander LJ, Stewart AF, Mackinlay AG, Kapelinskaya TV (1988) Isolation and characterization of the bovine kappa-casein gene. European Journal of Biochemistry 178, 395–401.
Isolation and characterization of the bovine kappa-casein gene.CrossRef | 1:CAS:528:DyaL1MXltV2qtrw%3D&md5=dfcdd667786818c56f078059d8baf803CAS |

Alipanah M, Kalashnikova L, Rodionov G (2005) Kappa-casein genotypic frequencies in Russian breeds Black and Red Pied cattle. Iranian Journal of Biotechnology 3, 191–194.

Azevedo ALS, Nascimento CS, Steinberg RS, Carvalho MRS, Peixoto MGCD, Teodoro RL, Verneque RS, Guimarães SEF, Machado MA (2008) Genetic polymorphism of the kappa-casein gene in Brazilian cattle. Genetics and Molecular Research 7, 623–630.
Genetic polymorphism of the kappa-casein gene in Brazilian cattle.CrossRef | 1:CAS:528:DC%2BD1cXhtFKrtb3I&md5=fff89ee96d2e2ef9e0749bba70400743CAS |

Beja-Pereira A, Erhardt G, Matos C, Gama L, Ferrand N (2002) Evidence for a geographical cline of casein haplotypes in Portuguese cattle breeds. Animal Genetics 33, 295–300.
Evidence for a geographical cline of casein haplotypes in Portuguese cattle breeds.CrossRef | 1:CAS:528:DC%2BD38XmvVWrurw%3D&md5=3fb086d6de14f08b6efef7b1e9a46595CAS |

Boettcher PJ, Caroli A, Stella A, Chessa S, Budelli E, Canavesi F, Ghiroldi S, Pagnacco G (2004) Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle. Journal of Dairy Science 87, 4311–4317.
Effects of casein haplotypes on milk production traits in Italian Holstein and Brown Swiss cattle.CrossRef | 1:CAS:528:DC%2BD2cXhtVCjtrfO&md5=29e32a6a84ccc84a04b43e41f07015b6CAS |

Bovenhuis H, Van Arendonk JA, Korver S (1992) Associations between milk protein polymorphisms and milk production traits. Journal of Dairy Science 75, 2549–2559.
Associations between milk protein polymorphisms and milk production traits.CrossRef | 1:STN:280:DyaK3s%2FosV2qsg%3D%3D&md5=99d04b1f2c2fb5e1f14a4dcc20d48b5aCAS |

Bugeac T, Balteanu VA, Creanga S (2013) Kappa-Casein Genetic Variants and Their Relationships with Milk Production and Quality in Montbéliarde Dairy Cows. Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies 70, 193–194.

Cardak AD (2005) Effects of genetic variants in milk protein on yield and composition of milk from Holstein-Friesian and Simmentaler cows. South African Journal of Animal Science 35, 41–47.

Caroli A, Chessa S, Bolla P, Budelli E, Gandini GC (2004) Genetic structure of milk protein polymorphisms and effects on milk production traits in a local dairy cattle. Journal of Animal Breeding and Genetics 121, 119–127.
Genetic structure of milk protein polymorphisms and effects on milk production traits in a local dairy cattle.CrossRef | 1:CAS:528:DC%2BD2cXktlChtrs%3D&md5=f599892e0a0ab619d48d0299c390b948CAS |

Caroli AM, Chessa S, Erhardt GJ (2009) Invited review: milk protein polymorphisms in cattle: effect on animal breeding and human nutrition. Journal of Dairy Science 92, 5335–5352.
Invited review: milk protein polymorphisms in cattle: effect on animal breeding and human nutrition.CrossRef | 1:CAS:528:DC%2BD1MXhtlKnsrnO&md5=c2ef24345ce44ddb5e8bd2fbdc3666e3CAS |

Di Stasio L, Mariani P (2000) The role of protein polymorphism in the genetic improvement of milk production. Zootecnica e Nutrizione Animale 26, 69–90.

Dogan M, Kaygısız A (1999) Relationships between milk yield traits and milk protein polymorphism in Brown Swiss cattle in Turkey. Turkish Journal of Veterinary and Animal Sciences 23, 47–49.

Dogru U, Ozdemir M (2009) Genotyping of Kappa-casein locus by PCR-RFLP in brown Swiss cattle breed. Journal of Animal and Veterinary Advances 8, 779–781.

El-Rafey GA, Darwish SF (2008) A PCR-RFLP assay to detect genetic variants of kappa-casein gene in cattle and buffalo. Arab Journal of Biotechnology 11, 11–18.

Farrell HM, Jimenez-Flores R, Bleck GT, Brown EM, Butler JE, Creamer LK, Hicks CL, Hollar CM, Ng-Kwai-Hang KF, Swaisgood HE (2004) Nomenclature of the proteins of cows’ milk-sixth revision. Journal of Dairy Science 87, 1641–1674.
Nomenclature of the proteins of cows’ milk-sixth revision.CrossRef | 1:CAS:528:DC%2BD2cXltlGnsrk%3D&md5=7d1d83bc173dd728ea0c4eb248abdddeCAS |

Ferretti L, Leone P, Sgaramella V (1990) Long range restriction analysis of the bovine casein genes. Nucleic Acids Research 18, 6829–6833.
Long range restriction analysis of the bovine casein genes.CrossRef | 1:CAS:528:DyaK3MXls1Ogsw%3D%3D&md5=a4bca7b2e7b9691443dd1e8b055ee703CAS |

Fox PF, McSweeney PLH (1998) ‘Dairy chemistry and biochemistry.’ (Blackie Academic and Professional: London)

Gallinat JL, Qanbari S, Drogemuller C, Pimentel ECG, Thaller G, Tetens J (2013) DNA-based identification of novel bovine casein gene variants. Journal of Dairy Science 96, 699–709.
DNA-based identification of novel bovine casein gene variants.CrossRef | 1:CAS:528:DC%2BC38XhvV2rt77N&md5=43f188c281ac314ff4464a1c044ff67fCAS |

Gurcan EK (2011) Association between milk protein polymorphism and milk production traits in Black and White dairy cattle in Turkey. African Journal of Biotechnology 10, 1044–1048.

Hallén E, Wedholm A, Andrén A, Lundén A (2008) Effect of β-casein, κ-casein and β-lactoglobulin genotypes on concentration of milk protein variants. Journal of Animal Breeding and Genetics 125, 119–129.
Effect of β-casein, κ-casein and β-lactoglobulin genotypes on concentration of milk protein variants.CrossRef |

Hamza AE, Yang ZP, Wang XL, Chen RJ, Wu HT, Ibrahim AI (2010) Kappa casein gene polymorphism and its impact on milk yield and reproductive performance traits of Chinese Holstein cattle. Agricultural Journal 5, 283–285.
Kappa casein gene polymorphism and its impact on milk yield and reproductive performance traits of Chinese Holstein cattle.CrossRef |

Ikonen T, Ojala M, Ruottinen O (1999) Associations between milk protein polymorphism and first lactation milk production traits in Finnish Ayrshire cows. Journal of Dairy Science 82, 1026–1033.
Associations between milk protein polymorphism and first lactation milk production traits in Finnish Ayrshire cows.CrossRef | 1:CAS:528:DyaK1MXjt1yksLk%3D&md5=40e88b4c43efd059f125183052a62645CAS |

Ilie DE, Magdin A, Sălăjeanu A, Neamt R, Vintilă I (2009) Influence of CSN3 marker on milk composition in Romanian Brown and Romanian Simmental cattle from SCDCB Arad. Scientific Papers Animal Science and Biotechnologies 42, 54–57.

Jann OC, Prinzenberg FM, Luikart G, Caroli A, Erhardt G (2004a) High polymorphism in the Kappa-casein (CSN3) gene from wild and domestic caprine species revealed by DNA sequencing. The Journal of Dairy Research 71, 188–195.
High polymorphism in the Kappa-casein (CSN3) gene from wild and domestic caprine species revealed by DNA sequencing.CrossRef | 1:CAS:528:DC%2BD2cXjs1Kjsbw%3D&md5=66ed4c63c8eced1fbe2cbf21b4aed3c5CAS |

Jann OC, Ibeagha-Awemu EM, Ozbeyaz C, Zaragoza P, Williams JL, Ajmone-Marsan P, Lenstra JA, Moazami-Goudarzi K, Erhardt G (2004b) Geographic distribution of haplotype diversity at the bovine casein locus. Genetics, Selection, Evolution. 36, 243–257.
Geographic distribution of haplotype diversity at the bovine casein locus.CrossRef | 1:CAS:528:DC%2BD2cXjslygu74%3D&md5=4daec6f5155363760bebb55d147a2556CAS |

Kimura M, Crow JF (1964) The number of alleles that can be maintained in a finite population. Genetics 49, 725–738.

Kucerova J, Matejicek A, Jandurová OM, Sorensen P, Nemcova E, Stipkova M, Kott T, Bouška J, Frelich J (2006) Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh. Czech Journal of Animal Science 51, 241–247.

Lien S, Kantanen J, Holm LE, Eythorsdottir E, Sandberg K, Dalsgard B, Adalsteinsson S (1999) Comparison of milk protein allele frequencies in Nordic cattle breeds. Animal Genetics 30, 85–91.
Comparison of milk protein allele frequencies in Nordic cattle breeds.CrossRef | 1:STN:280:DyaK1MzgsVGrtA%3D%3D&md5=8d98bd2568295270e8eed8cf637d0e62CAS |

Manga I, Riha J, Dvorak J (2006) Comparison of influence markers CSN3 and CSN2 on milk performance traits in Czech Spotted and Holstein cattle tested at first, fifth and higher lactation. Acta Fytotechnica et Zootechnica 13, 13–15.

Martin P, Szymanowska M, Zwierzchowski L, Leroux C (2002) The impact of genetic polymorphisms on the protein composition of ruminants milks. Reproduction, Nutrition, Development 42, 433–459.
The impact of genetic polymorphisms on the protein composition of ruminants milks.CrossRef | 1:CAS:528:DC%2BD3sXps1OltA%3D%3D&md5=810857dbae9eb1df58213d8827855101CAS |

Mercier JC, Vilotte JL, Provost C (1990) Structure and function of milk protein genes. In ‘Genome analysis in domestic animals’. (Eds H Geldermann, F Ellendorf) pp. 233–258. (Verlag Chemie: Weinheim)

Miciński J, Klupczyński J (2006) Correlations between polymorphic variants of milk proteins, and milk yield and chemical composition in Black-and-White and Jersey cows. Polish Journal of Food and Nutrition Sciences 56, 137–145.

Mohammadi Y, Aslaminejad AA, Nassiry MR, Koshkoieh AE (2013) Allelic polymorphism of K-casein, β-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle. Journal of Cell and Molecular Research 5, 75–80.

Monaci L, Tregoat V, van Hengel AJ, Anklam E (2006) Milk allergens, their characteristics and their detection in food: a review. European Food Research and Technology 223, 149–179.

Neelin JM (1964) Variants of K-casein revealed by improved starch gel electrophoresis. Journal of Dairy Science 47, 506–509.
Variants of K-casein revealed by improved starch gel electrophoresis.CrossRef | 1:CAS:528:DyaF2cXktVGhurk%3D&md5=62a786fc920753326992ba7cdee5940fCAS |

Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70, 3321–3323.
Analysis of gene diversity in subdivided populations.CrossRef | 1:STN:280:DyaE2c%2FlsFCrtQ%3D%3D&md5=2db685de57de066bc3c0363874e7bf06CAS |

Ng-Kwai-Hang KF, Monardes HG, Hayes JF (1990) Association between genetic polymorphism of milk proteins and production traits during three lactations. Journal of Dairy Science 73, 3414–3420.
Association between genetic polymorphism of milk proteins and production traits during three lactations.CrossRef | 1:CAS:528:DyaK3MXisVKisL0%3D&md5=5ca28afe3335f4854e8279e97f17a056CAS |

Ozdemir M, Dogru U (2005) Relationship between Kappa-casein polymorphism and production traits in Brown Swiss and Holstein. Journal of Applied Animal Research 27, 101–104.
Relationship between Kappa-casein polymorphism and production traits in Brown Swiss and Holstein.CrossRef | 1:CAS:528:DC%2BD2MXhtFWqs7zI&md5=8657f8ef03143d2472d493fb297fb34bCAS |

Prinzenberg EM, Krause I, Erhardt G (1999) SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1). Animal Biotechnology 10, 49–62.
SSCP analysis at the bovine CSN3 locus discriminates six alleles corresponding to known protein variants (A, B, C, E, F, G) and three new DNA polymorphisms (H, I, A1).CrossRef | 1:CAS:528:DyaK1MXmslyku7k%3D&md5=0306e8451d046953de5f05dd2ce7a95fCAS |

Sargent FD, Lytton VH, Wall OG (1968) Test interval method of calculating Dairy Herd Improvement Association records. Journal of Dairy Science 51, 170–179.
Test interval method of calculating Dairy Herd Improvement Association records.CrossRef |

Sitkowska B, Wojciech N, Ewa W (2008) Relations between kappa-casein polymorphism (CSN3) and milk performance traits in heifer cows. Journal of Central European Agriculture 9, 641–644.

Soria LA, Iglesias GM, Huguet MJ, Mirande SL (2003) A PCR-RFLP test to detect allelic variants of the Bovine Kappa-Casein gene. Animal Biotechnology 14, 1–5.
A PCR-RFLP test to detect allelic variants of the Bovine Kappa-Casein gene.CrossRef | 1:CAS:528:DC%2BD3sXmsVWrsLs%3D&md5=4b3ab20fe7218ecb697515f33d98a6b8CAS |

Tsiaras AM, Bargouli GG, Banos G, Boscos CM (2005) Effect of kappa-casein and beta-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. Journal of Dairy Science 88, 327–334.
Effect of kappa-casein and beta-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows.CrossRef | 1:CAS:528:DC%2BD2MXmslSr&md5=9fe4c7de394e1cbf69d18e427549b4ceCAS |

Yahyaoui MH (2003) Genetic polymorphism in goat. PhD thesis, Universidad Autonoma de Barcelona, Spain.

Yeh FC, Yang RC, Boyle T, Ye ZH, Mao JX (1999) ‘Popgene, version 1.32: the user friendly software for population genetic analysis.’ (Molecular Biology and Biotechnology Centre, University of Alberta: Edmonton, AB)



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