Supplementation with crushed rapeseed causes reduction of methane emissions from lactating dairy cows on pasture
T. M. Storlien A C , E. Prestløkken A , K. A. Beauchemin B , T. A. McAllister B , A. Iwaasa B and O. M. Harstad A
+ Author Affiliations
- Author Affiliations
A Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, PO Box 5003, 1432 Ås, Norway.
B Agriculture and Agri-Food Canada, Research Centre, 5403 1st Avenue South, Lethbridge, Alberta, Canada T1J 4B1.
C Corresponding author. Email: tonje.storlien@nmbu.no
Animal Production Science 57(1) 81-89 https://doi.org/10.1071/AN15287
Submitted: 5 June 2015 Accepted: 6 August 2015 Published: 23 October 2015
Abstract
The main objective of this study was to investigate the effect of supplementing a pasture diet with crushed rapeseed on enteric methane (CH4) emissions from lactating dairy cows. The experiment was conducted as a crossover design using eight multiparous lactating Norwegian red dairy cows [(means ± s.d.) 548 ± 52 kg bodyweight, 38 ± 14 days in milk and 35 ± 3.7 kg milk/day, at the start of the experiment] maintained in two groups and fed two diets in two periods with the second period extended (18 days) to investigate the persistence of the CH4 response. Four of the eight cows were fitted with a rumen cannula with two cannulated cows assigned to each group. Cows were maintained on pasture (24 h/day) with access to 9 kg/day of concentrate containing 10% crushed rapeseed (RSC) or a control concentrate (CC). Dietary fat content was 63 g/kg dry matter for RSC and 42 g/kg dry matter for CC. The CH4 production was measured for five consecutive days in each period using the sulfur hexafluoride tracer gas technique. Compared with CC, RSC caused a reduction in enteric CH4 emission (221 vs 251 g/day and 8.1 vs 9.0 g/kg of energy-corrected milk), and this response persisted in the extension period. Cows fed RSC had higher milk yield compared with cows fed CC (31.7 vs 29.6 kg/day). However, milk fat and protein content were lower in milk from cows fed RSC than CC. Therefore, energy-corrected milk was not affected by treatment. Feeding RSC lowered milk fat content of palmitic acid compared with CC. The study showed that adding crushed RSC to the diet can be an effective means of reducing CH4 emissions from lactating dairy cows on pasture, without negatively affecting milk production.
Additional keywords: cattle, dairy performance, fat, greenhouse gases.
References
AACC (1995) AACC International. Approved Methods of Analysis, 11th edn. Method 76–13.01. Total Starch Assay Procedure (Megazyme Amyloglucosidase/alpha-Amylase Method). (AACC, International: St Paul, MN) Available at http://methods.aaccnet.org/summaries/76-13-01.aspx [Verified 10 May 2015]Alstrup L, Hellwing ALF, Lund P, Weisbjerg MR (2015) Effect of fat supplementation and stage of lactation on methane emission in dairy cows. Animal Feed Science and Technology 207, 10–19.
| Effect of fat supplementation and stage of lactation on methane emission in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXpsVWrsr8%3D&md5=610a6621e524f364616e112798488c65CAS |
AOAC (1990) Official method No. 984.13. In ‘Official method of analysis’. 15th edn. (Ed. K Helrich) pp. 191–201. (Association of Official Analytical Chemists Inc.: Arlington, VA)
Bauman DE, Griinari JM (2000) Historical perspective and recent developments in identifying the cause of diet-induced milk fat depression. In ‘Proceedings of the Cornell Nutrition Conference for Feed Manufacturers, Rochester, NY’. pp. 191–201. (Cornell University: Ithaca, NY)
Beauchemin KA, Kreuzer M, O’Mara F, McAllister TA (2008) Nutritional management for enteric methane abatement: a review. Australian Journal of Experimental Agriculture 48, 21–27.
| Nutritional management for enteric methane abatement: a review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXovVGn&md5=6ae1cf9889bdef55e5354cf57109b8ccCAS |
Beauchemin KA, McGinn SM, Benchaar C, Holtshausen L (2009) Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production. Journal of Dairy Science 92, 2118–2127.
| Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXlsFyms74%3D&md5=a1a417f31ec0c089461cd5bb19e08a6aCAS | 19389969PubMed |
Berndt A, Boland TM, Deighton MH, Gere JI, Grainger C, Hegarty RS, Iwaasa AD, Koolaard JP, Lassey KR, Luo D, Martin RJ, Martin C, Moate PJ, Molano G, Pinares-Patiño C, Ribaux BE, Swainson NM, Waghorn GC, Williams SRO (2014) Guidelines for use of sulphur hexafluoride (SF6) tracer technique to measure enteric methane emissions from ruminants. (Ed. MG Lambert) (New Zealand Agricultural Greenhouse Gas Research Centre: Wellington, New Zealand)
Brask M, Lund P, Hellwing ALF, Poulsen M, Weisbjerg MR (2013a) Enteric methane production, digestibility and rumen fermentation in dairy cows fed different forages with and without rapeseed fat supplementation. Animal Feed Science and Technology 184, 67–79.
| Enteric methane production, digestibility and rumen fermentation in dairy cows fed different forages with and without rapeseed fat supplementation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtV2rur%2FE&md5=157f1ec023320509be0ca73ed8bdb311CAS |
Brask M, Lund P, Weisbjerg MR, Hellwing ALF, Poulsen M, Larsen MK, Hvelplund T (2013b) Methane production and digestion of different physical forms of rapeseed as fat supplements in dairy cows. Journal of Dairy Science 96, 2356–2365.
| Methane production and digestion of different physical forms of rapeseed as fat supplements in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXis1Oktr0%3D&md5=8b6f84aba1db199bbcde438b70a2e9e6CAS | 23415515PubMed |
Chilliard Y, Ferlay A (2004) Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties. Reproduction, Nutrition, Development 44, 467–492.
| Dietary lipids and forages interactions on cow and goat milk fatty acid composition and sensory properties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXmvVartg%3D%3D&md5=1f08b33e5473787e778e9295774d10bfCAS | 15636165PubMed |
Chilliard Y, Glasser F, Ferlay A, Bernard L, Roul J, Doreau M (2007) Diet, rumen biohyrogenation and nutritional quality of cow and goat milk fat. European Journal of Lipid Science and Technology 109, 828–855.
| Diet, rumen biohyrogenation and nutritional quality of cow and goat milk fat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtVSnsrrO&md5=10d8688c2ae3f09c74414815dc5aad7aCAS |
Collomb M, Sollberger H, Bütikofer U, Sieber R, Stoll W, Schaeren W (2004) Impact of a basal diet of hay and fodder beet supplemented with rapeseed, linseed and sunflowerseed on the fatty acid composition of milk fat. International Dairy Journal 14, 549–559.
| Impact of a basal diet of hay and fodder beet supplemented with rapeseed, linseed and sunflowerseed on the fatty acid composition of milk fat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXisVSrsLo%3D&md5=405dca6a1cb8274ef97c46b980d8796bCAS |
Dijkstra J, Ellis JL, Kebreab E, Strathe AB, López S, France J, Bannink A (2012) Ruminal pH regulation and nutritional consequences of low pH. Animal Feed Science and Technology 172, 22–33.
| Ruminal pH regulation and nutritional consequences of low pH.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XitVCgt7s%3D&md5=09b67c5c32584f94f29dbf32a4dee620CAS |
Dionex (2010a) ‘Application Note 345: extraction of fat from dairy products (cheese, butter, and liquid milks) using accelerated solvent extraction (ASE).’ (Thermo Fischer Scientific Inc., Sunnyvale, CA)
Dionex (2010b) Technical Note 209: Accelerated solvent extraction (ASE®) sample preparation techniques for food and animal feed samples. Available at http://www.dionex.com/en-us/webdocs/36395-TN209_V28_releasedJC042606.pdf [Verified 10 May 2015]
Dubois V, Breton S, Linder M, Fanni J, Parmentier M (2007) Fatty acid profiles of 80 vegetable oils with regard to their nutritional potential. European Journal of Lipid Science and Technology 109, 710–732.
| Fatty acid profiles of 80 vegetable oils with regard to their nutritional potential.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXovFalsLo%3D&md5=9182e5ae2f4999af055e05f6f0408380CAS |
France J, Siddons RC (1993) Volatile fatty acid production. In ‘Quantitative aspects of ruminant digestion and metabolism’. (Eds JM Forbes, J France) pp. 107–121. (CAB International: New York)
Gerber PJ, Steinfeld H, Henderson B, Mottet A, Opio C, Dijkman J, Falcucci A, Tempio G (2013) ‘Tackling climate change through livestock – a global assessment of emissions and mitigation opportunities.’ (Food and Agriculture Organization of the United Nations (FAO): Rome)
Glasser F, Ferlay A, Chilliard Y (2008) Oilseed lipid supplements and fatty acid composition of cow milk: a meta-analysis. Journal of Dairy Science 91, 4687–4703.
| Oilseed lipid supplements and fatty acid composition of cow milk: a meta-analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsVKgtrvE&md5=485ab5d3ce4f0cb781ff3aa0525a0632CAS | 19038946PubMed |
Grainger C, Beauchemin KA (2011) Can enteric methane emissions from ruminants be lowered without lowering their production? Animal Feed Science and Technology 166–167, 308–320.
| Can enteric methane emissions from ruminants be lowered without lowering their production?Crossref | GoogleScholarGoogle Scholar |
Hellwing ALF, Sørensen MT, Weisbjerg MR, Vestergaard M, Lund P (2012) Can rapeseed lower methane emission from heifers? Acta Agriculturae Scandinavica, Section A – Animal Science 62, 259–262.
Hook SE, Wright ADG, McBride BW (2010) Methanogens: methane producers of the rumen and mitigation strategies. Archaea (Vancouver, B.C.) 2010, 945785
| Methanogens: methane producers of the rumen and mitigation strategies.Crossref | GoogleScholarGoogle Scholar |
Hristov AN, Oh J, Firkins J, Dijkstra J, Kebreab E, Waghorn G, Makkar HPS, Adesogan AT, Yang W, Lee C, Gerber PJ, Henderson B, Tricarico JM (2013) Mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options. Journal of Animal Science 91, 5045–5069.
| Mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhslKktrrL&md5=232196d68807bd4a5064908acacdfea8CAS | 24045497PubMed |
Hünerberg M, McGinn SM, Beaucheming KA, Entz T, Okine EK, Harstad OM, McAllister TA (2015) Impact of ruminal pH on enteric methane emissions. Journal of Animal Science 93, 1760–1766.
| Impact of ruminal pH on enteric methane emissions.Crossref | GoogleScholarGoogle Scholar | 26020197PubMed |
ISO 1211 (2010) Milk – determination of fat content – gravimetric method (reference method).
ISO 5984 (2002) Animal feeding stuffs – determination of crude ash.
ISO 6496 (1999) Animal feeding stuffs – determination of moisture and other volatile matter content.
Iwaasa AD, Stumborg MA, Wittenberg KM, McGinn SM, McAllister TA (2004) Development of a cost effective and simple sampling apparatus and PVC collection yoke system for methane measurement from grazing ruminants using the SF6 technique. Canadian Journal of Animal Science 84, 775
Janssen PH (2010) Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics. Animal Feed Science and Technology 160, 1–22.
| Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtV2itLvF&md5=cd6e6927f396985cb5d2142830b3b783CAS |
Johnson K, Huyler M, Westberg H, Lamb B, Zimmerman P (1994) Measurement of methane emission from ruminant livestock using a SF6 tracer technique. Environmental Science & Technology 28, 359–362.
| Measurement of methane emission from ruminant livestock using a SF6 tracer technique.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2cXmtFahtA%3D%3D&md5=0064e1a5426af33063e026a8886af305CAS |
Johnson KA, Kincaid RL, Westberg HH, Gaskins CT, Lamb BK, Cronrath JD (2002) The effect of oilseeds in diets of lactating cows on milk production and methane emissions. Journal of Dairy Science 85, 1509–1515.
| The effect of oilseeds in diets of lactating cows on milk production and methane emissions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xlt1Srsbc%3D&md5=47cc1d7e33415c926d29c64ce448d01dCAS | 12146483PubMed |
Knapp JR, Laur GL, Vadas PA, Weiss WP, Tricarico JM (2014) Invited review: enteric methane in dairy cattle production: quantifying the opportunities and impact of reducing emissions. Journal of Dairy Science 97, 3231–3261.
| Invited review: enteric methane in dairy cattle production: quantifying the opportunities and impact of reducing emissions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmtlCnt74%3D&md5=a988b8ca75867d19f75b1203819a4b32CAS | 24746124PubMed |
Kolver ES, de Veth MJ (2002) Prediction of ruminal pH from pasture-based diets. Journal of Dairy Science 85, 1255–1266.
| Prediction of ruminal pH from pasture-based diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XktFWjs74%3D&md5=fd8c1c762e3d6438ad6dc812462eea64CAS | 12086063PubMed |
Kramer JK, Fellner V, Dugan ME, Sauer FD, Mossoba MM, Yurawecz MP (1997) Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids. Lipids 32, 1219–1228.
| Evaluating acid and base catalysts in the methylation of milk and rumen fatty acids with special emphasis on conjugated dienes and total trans fatty acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXnsFaisbk%3D&md5=926def23449222b22684e7a9a17daa50CAS | 9397408PubMed |
Martin C, Morgavi DP, Doreau M (2010) Methane mitigation in ruminants: from microbe to the farm scale. Animal 4, 351–365.
| Methane mitigation in ruminants: from microbe to the farm scale.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhslWgs7k%3D&md5=ec86c02a2eac08f6c5be320152290e71CAS | 22443940PubMed |
Meale SJ, McAllister TA, Beauchemin KA, Harstad OM, Chaves AV (2012) Strategies to reduce greenhouse gases from ruminant livestock. Acta Agriculturae Scandinavica, Section A – Animal Science 62, 199–211.
Meale SJ, Chaves AV, McAllister TA, Iwaasa AD, Yang WZ, Benchaar C (2014) Including essential oils in lactating dairy cow diets: effects on methane emissions. Animal Production Science 54, 1215–1218.
Mertens DR, Allen M, Carmany J, Clegg J, Davidowicz A, Drouches M, Frank K, Gambin D, Garkie M, Gildemeister B, Jeffress D, Jeon C-S, Jones D, Kaplan D, Kim G-N, Kobata S, Main D, Moua X, Paul B, Robertson J, Taysom D, Thiex N, Williams J, Wolf M (2002) Gravimetric determination of amylase-treated neutral detergent fiber in feeds with refluxing in beakers or crucibles: collaborative study. Journal of AOAC International 85, 1217–1240.
Norwegian Feed Table (2008) (University of Life Science, Ås, Norway) Available at http://statisk.umb.no/iha/fortabell/index.php [Verified 1 June 2015]
NRC (2001) ‘Nutrient requirements of dairy cattle.’ 7th revised edn. Subcommittee on Dairy Cattle Nutrition, Committee on Animal Nutrition, National Research Council. pp. 28–33. (National Academy Press: Washington, DC)
O’Fallon JV, Busboom JR, Nelson ML, Gaskins CT (2007) A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs. Journal of Animal Science 85, 1511–1521.
| A direct method for fatty acid methyl ester synthesis: application to wet meat tissues, oils, and feedstuffs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXls1antL0%3D&md5=63c85445b874abb6a7a8b2e8b070a5c3CAS | 17296772PubMed |
Pinares-Patino CS, Franco FE, Molano G, MacLean S, Kjestrup H, Sandoval E, Laubach J (2013) Methane emissions from cattle grazed on pasture sprayed with canola oil. In ‘Proceedings of the 5th greenhouse gases and animal agriculture conference (GGAA 2013)’. Vol. 4, 273. (Eds F O’Mara, T Boland) (Cambridge University Press: Cambridge)
SAS Institute Inc. (2011) ‘SAS/STAT user’s guide.’ (SAS Institute Inc.: Cary, NC)
Shingfield KJ, Garnsworthy PC (2012) Rumen lipid metabolism and its impact on milk production and quality. In ‘Revent advances in animal nutrition 2012’. (Eds PC Garnsworthy, J Wiseman) pp. 53–94. (Nottingham University Press: Nottingham)
Sjaunja LO, Baevre L, Junkkarinen L, Pedersen J, Setala J (1991) A Nordic proposal for an energy corrected milk formula. In ‘EAAp Publication 50: performance recording of animals-state of the art 1990’. (Eds P Gaillon, Y Chabert) pp. 156–157. (Centre for Agricultural Publishing and Documentation (PUDOC): Wageningen, The Netherlands)
Taugbøl O, Karlengen IJ, Salbu B, Aastveit AH, Harstad OM (2010) Intravenous injections of cobalt reduce fatty acid desaturation products in milk and blood of lactating cows. Journal of Animal Physiology and Animal Nutrition 94, 635–640.
| Intravenous injections of cobalt reduce fatty acid desaturation products in milk and blood of lactating cows.Crossref | GoogleScholarGoogle Scholar | 20050955PubMed |
Van Kessel JAS, Russell JB (1996) The effect of pH on ruminal methanogenesis. FEMS Microbiology Ecology 20, 205–210.
| The effect of pH on ruminal methanogenesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XkslGiur4%3D&md5=37d25e000980c8cf82bfb106a2f3a5d5CAS |
Volden H (2011) NorFor – the Nordic feed evaluation system. EAAP Publication No. 130. (Wageningen Academic Publishers: AE Wageningen, The Netherlands)
Wims CM, Deighton MH, Lewis E, O’Loughlin B, Delaby L, Boland TM, O’Donovan M (2010) Effect of pregrazing herbage mass on methane production, dry matter intake, and milk production of grazing dairy cows during the mid-season period1. Journal of Dairy Science 93, 4976–4985.
| Effect of pregrazing herbage mass on methane production, dry matter intake, and milk production of grazing dairy cows during the mid-season period1.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFKmsb%2FN&md5=636203d59bad529279315f5c8aa68fbfCAS | 20855032PubMed |
Woodward S, Waghorn GC, Thomson NA (2006) Supplementing dairy cows with oils to improve performance and reduce methane? Does it work? Proceedings of the New Zealand Society of Animal Production 66, 176–181.
