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Food, fibre and pharmaceuticals from animals
REVIEW

Digestibility of calcium in feed ingredients and requirements of digestible calcium for growing pigs

J. C. González-Vega A and H. H. Stein A B
+ Author Affiliations
- Author Affiliations

A Department of Animal Sciences, University of Illinois, Urbana, IL 61801, USA.*

B Corresponding author. Email: hstein@illinois.edu

Animal Production Science 56(8) 1339-1344 https://doi.org/10.1071/AN15352
Submitted: 7 July 2015  Accepted: 3 December 2015   Published: 20 April 2016

Abstract

Efforts to reduce phosphorus (P) excretion from pigs have increased during the past few decades and it has been recognised that interactions among dietary P, calcium (Ca), phytate, and microbial phytase exist. However, limited research has been reported on Ca digestibility, but to optimise the use of both Ca and P, digestibility values of Ca are needed. Due to endogenous losses of Ca, values for standardised total tract digestibility (STTD) of Ca in different Ca supplements and feed ingredients have been determined, and these values may be used to formulate mixed diets. Phytate may bind intrinsic Ca in feed ingredients of plant origin as well as extrinsic Ca from ingredients of animal origin or Ca supplements, but not all forms of Ca in Ca supplements will bind to phytate. Therefore, the effect of phytase on the STTD of Ca may vary depending on the amount of Ca bound to phytate and in some cases microbial phytase will result in increased STTD of Ca from animal proteins or Ca supplements. Dietary fibre may increase the STTD of Ca, but particle size and soybean oil do not influence the STTD of Ca. Requirements for digestible Ca by growing pigs has not yet been determined, but with the availability of values for the STTD of Ca in most commonly used feed ingredients, the basis for determining such values has been prepared. In conclusion, data for the STTD of Ca and the effects of microbial phytase in many feed ingredients have been determined and future research will be directed at determining the requirements for digestible Ca by different groups of pigs.

Additional keywords: microbial phytase, phytate, pig.


References

Agnew JE, Holdsworth CD (1971) The effect of fat on calcium absorption from a mixed meal in normal subjects, patients with malabsorptive disease, and patients with a partial gastrectomy. Gut 12, 973–977.
The effect of fat on calcium absorption from a mixed meal in normal subjects, patients with malabsorptive disease, and patients with a partial gastrectomy.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaE387gtlCitw%3D%3D&md5=ac21ac27d372fc4f70eef98d6a7ec632CAS | 5003268PubMed |

Akinmusire AS, Adeola O (2009) True digestibility of phosphorus in canola and soybean meals for growing pigs: influence of microbial phytase. Journal of Animal Science 87, 977–983.
True digestibility of phosphorus in canola and soybean meals for growing pigs: influence of microbial phytase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXislaru7w%3D&md5=4578af823c05b14377fb9883bbfbcd06CAS | 19028861PubMed |

Almeida FN, Stein HH (2010) Performance and phosphorus balance of pigs fed diets formulated on the basis of values for standardized total tract digestibility of phosphorus. Journal of Animal Science 88, 2968–2977.
Performance and phosphorus balance of pigs fed diets formulated on the basis of values for standardized total tract digestibility of phosphorus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtFSktb3L&md5=f67eb3b469112dd047debeb485a27a34CAS | 20495131PubMed |

Alonso R, Rubio LA, Muzquiz M, Marzo F (2001) The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals. Animal Feed Science and Technology 94, 1–13.
The effect of extrusion cooking on mineral bioavailability in pea and kidney bean seed meals.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXptVyht7o%3D&md5=474ef45c0eae216ca89ace9993e4d4b2CAS |

Ammerman CB (1995) Methods for estimation of mineral bioavailability. In ‘Bioavailability of nutrients for animals: amino acids, minerals, and vitamins’. (Eds CB Ammerman, DH Baker, AJ Lewis) pp. 83–94. (Academic Press: San Diego, CA)

Araujo JA, da Silva JHV, Costa FGP, de Sousa JMB, Givisiez PEN, Sakomura NK (2011) Effect of the levels of calcium and particle size of limestone on laying hens. Revista Brasileira de Zootecnia 40, 997–1005.
Effect of the levels of calcium and particle size of limestone on laying hens.Crossref | GoogleScholarGoogle Scholar |

Bendsen NT, Hother A-L, Jensen SK, Lorenzen JK, Astrup A (2008) Effect of dairy calcium on fecal fat excretion: a randomized crossover trial. International Journal of Obesity 32, 1816–1824.
Effect of dairy calcium on fecal fat excretion: a randomized crossover trial.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhsV2rsL%2FI&md5=9f303ef1b9761bfb8de6e7988f40fb2aCAS | 18838979PubMed |

Bird AR, Hayakawa T, Marsono Y, Gooden JM, Record IR, Correll RL, Topping DL (2000) Coarse brown rice increases fecal and large bowel short-chain fatty acids and starch but lowers calcium in the large bowel of pigs. The Journal of Nutrition 130, 1780–1787.

Bohlke RA, Thaler RC, Stein HH (2005) Calcium, phosphorus, and amino acid digestibility in low-phytate corn, normal corn, and soybean meal by growing pigs. Journal of Animal Science 83, 2396–2403.

Boyd OF, Crum CL, Lyman JF (1932) The absorption of calcium soaps and the relation of dietary fat to calcium utilization in the white rat. The Journal of Biological Chemistry 95, 29–41.

Brady SM, Callan JJ, Cowan D, McGrane M, O’Doherty JV (2002) Effect of phytase inclusion and calcium/phosphorus ratio on the performance and nutrient retention of grower-finisher pigs fed barley/wheat/soya bean meal-based diets. Journal of the Science of Food and Agriculture 82, 1780–1790.
Effect of phytase inclusion and calcium/phosphorus ratio on the performance and nutrient retention of grower-finisher pigs fed barley/wheat/soya bean meal-based diets.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XptlGktbY%3D&md5=227041fafc5f649ef6e3e5ca8fe7964fCAS |

Carpenter S, Bennet E (2011) Reconsideration of the planetary boundary for phosphorus. Environmental Research Letters 6, 014009
Reconsideration of the planetary boundary for phosphorus.Crossref | GoogleScholarGoogle Scholar |

Cervantes-Pahm SK, Stein HH (2008) Effect of dietary soybean oil and soybean protein concentration on the concentration of digestible amino acids in soybean products fed to growing pigs. Journal of Animal Science 86, 1841–1849.
Effect of dietary soybean oil and soybean protein concentration on the concentration of digestible amino acids in soybean products fed to growing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXpt12hsL0%3D&md5=68590f7d25c2e836159ae6f063911079CAS | 18407995PubMed |

Christakos S (2012) Recent advances in our understanding of 1,25-dihydroxyvitamin D3 regulation of intestinal calcium absorption. Archives of Biochemistry and Biophysics 523, 73–76.
Recent advances in our understanding of 1,25-dihydroxyvitamin D3 regulation of intestinal calcium absorption.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xns1CjtLY%3D&md5=8ce87f1566c2622e15227a9549793a14CAS | 22230327PubMed |

Clark I (1969) Importance of dietary Ca:PO4 ratios on skeletal, Ca, Mg, and PO4 metabolism. The American Journal of Physiology 217, 865–870.

Costanzo LS (2006) Endocrine physiology. In ‘Physiology’. 3rd edn. pp. 377–439. (Saunders Elsevier: Philadelphia, PA)

Coudray C, Bellanger J, Castiglia-Delavaud C, Rémésy C, Vermorel M, Rayssignuier Y (1997) Effects of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men. European Journal of Clinical Nutrition 51, 375–380.
Effects of soluble or partly soluble dietary fibres supplementation on absorption and balance of calcium, magnesium, iron and zinc in healthy young men.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXns1yisr8%3D&md5=eb7bd53d41f5214dfa2066d2bad5527dCAS | 9192195PubMed |

Debon SJJ, Tester RF (2001) In vitro binding of calcium, iron and zinc by non-starch polysaccharides. Food Chemistry 73, 401–410.
In vitro binding of calcium, iron and zinc by non-starch polysaccharides.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXjt12ns7c%3D&md5=aa5a227a0563c50a93a8129d159a6975CAS |

Delgado-Andrade C, Rufián-Henares JA, Nieto R, Aguilera JF, Navarro MP, Seiquer I (2010) Does the pelleting process affect the nutritive value of a pre-starter diet for sucking piglets? Ex vivo studies on mineral absorption. Journal of the Science of Food and Agriculture 90, 898–905.

FAO (2011) ‘The state of the world’s land and water resources for food and agriculture (SOLAW): managing systems at risk.’ (Food and Agriculture Organization of the United Nations: Rome; and Earthscan: London)

Fisher H (1992) Low-calcium diets enhance phytate-phosphorus availability. Nutrition Reviews 50, 170–171.
Low-calcium diets enhance phytate-phosphorus availability.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3s%2FisF2hsQ%3D%3D&md5=91c64c8b169c72bb4a055500ffa94f06CAS | 1407746PubMed |

González-Vega JC, Walk CL, Liu Y, Stein HH (2013) Determination of endogenous intestinal losses of Ca and true total tract digestibility of calcium in canola meal fed to growing pigs. Journal of Animal Science 91, 4807–4816.
Determination of endogenous intestinal losses of Ca and true total tract digestibility of calcium in canola meal fed to growing pigs.Crossref | GoogleScholarGoogle Scholar | 23942713PubMed |

González-Vega JC, Walk CL, Liu Y, Stein HH (2014) The site of net absorption of Ca from the intestinal tract of growing pigs and effect of phytic acid, Ca level and Ca source on Ca digestibility. Archives of Animal Nutrition 68, 126–142.
The site of net absorption of Ca from the intestinal tract of growing pigs and effect of phytic acid, Ca level and Ca source on Ca digestibility.Crossref | GoogleScholarGoogle Scholar | 24646151PubMed |

González-Vega JC, Walk CL, Stein HH (2015a) Effects of microbial phytase on apparent and standardized total tract digestibility of calcium in calcium supplements fed to growing pigs. Journal of Animal Science 93, 2255–2264.
Effects of microbial phytase on apparent and standardized total tract digestibility of calcium in calcium supplements fed to growing pigs.Crossref | GoogleScholarGoogle Scholar | 26020322PubMed |

González-Vega JC, Walk CL, Stein HH (2015b) Effect of microbial phytase, fiber, and soybean oil on calculated values for apparent and standardized total tract digestibility of calcium in fish meal. Journal of Animal Science 93, 4808–4818.
Effect of microbial phytase, fiber, and soybean oil on calculated values for apparent and standardized total tract digestibility of calcium in fish meal.Crossref | GoogleScholarGoogle Scholar | 26523574PubMed |

González-Vega JC, Walk CL, Stein HH (2015c) Digestible calcium requirements and calcium and phosphorus balance for weanling pigs. Journal of Animal Science 93, 51–52.

Hafeez A, Mader A, Boroojeni FG, Ruhnke I, Röhe I, Männer K, Zentek J (2014) Impact of thermal and organic acid treatment of feed on apparent ileal mineral absorption, tibial and liver mineral concentration, and tibia quality in broilers. Poultry Science 93, 1754–1763.
Impact of thermal and organic acid treatment of feed on apparent ileal mineral absorption, tibial and liver mineral concentration, and tibia quality in broilers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXht1Clt7fO&md5=dfb63930c6961940eff44b7a5ef39ec6CAS | 24864282PubMed |

James WP, Branch WJ, Southgate DA (1978) Calcium binding by dietary fibre. Lancet 311, 638–639.
Calcium binding by dietary fibre.Crossref | GoogleScholarGoogle Scholar |

Kaune R (1996) Mechanism of intestinal calcium absorption and availability of dietary calcium in pigs. Dtsch Tieraztl Wochenschr 103, 215–218.

Kil DY, Stein HH (2011) Dietary soybean oil and choice white grease improve apparent ileal digestibility of amino acids in swine diets containing corn, soybean meal, and distillers dried grains with solubles. Revista Colombiana de Ciencias Pecuarias 24, 248–253.

Kutuzova GD, DeLuca HF (2004) Gene expression profiles in rat intestine identify pathways for 1,25-dihydroxyvitamin D3 stimulated calcium absorption and clarify its immunomodulatory properties. Archives of Biochemistry and Biophysics 432, 152–166.
Gene expression profiles in rat intestine identify pathways for 1,25-dihydroxyvitamin D3 stimulated calcium absorption and clarify its immunomodulatory properties.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXpslWnsrc%3D&md5=cc00f9f56df4b287513cd99ac2b947b7CAS | 15542054PubMed |

Lantzsch HJ, Wjst S, Drochner W (1995) The effect of dietary calcium on the efficacy of microbial phytase in rations for growing pigs. Journal of Animal Physiology and Animal Nutrition 73, 19–26.
The effect of dietary calcium on the efficacy of microbial phytase in rations for growing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXks1yns74%3D&md5=748c7e366b65c134160a7927c3383e15CAS |

Lei XG, Ku PK, Miller ER, Yokoyama MT, Ullrey DE (1994) Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weanling pigs. Journal of Animal Science 72, 139–143.

Liu J, Bollinger DW, Ledoux DR, Veum TL (2000) Effects of dietary calcium:phosphorus ratios on apparent absorption of calcium and phosphorus in the small intestine, cecum, and colon of pigs. Journal of Animal Science 78, 106–109.

Merriman LA, Stein HH (2015) Effect of particle size in calcium carbonate on apparent and standardized total tract digestibility and retention of calcium by growing pigs. Journal of Animal Science 93, 52

Miyada T, Nakajima A, Ebihara K (2011) Iron bound to pectin is utilised by rats. British Journal of Nutrition 106, 73–78.
Iron bound to pectin is utilised by rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXosVOlsrs%3D&md5=b90b18a642ecc209e05327cee9dad15eCAS | 21521538PubMed |

Montagne L, Pluske JR, Hampson DJ (2003) A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Animal Feed Science and Technology 108, 95–117.
A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals.Crossref | GoogleScholarGoogle Scholar |

Moore JH, Tyler C (1955a) Studies on the intestinal absorption and excretion of calcium and phosphorus in the pig. 1. A critical study of the Bergeim technique for investigating the intestinal absorption and excretion of calcium and phosphorus. British Journal of Nutrition 9, 63–80.
Studies on the intestinal absorption and excretion of calcium and phosphorus in the pig. 1. A critical study of the Bergeim technique for investigating the intestinal absorption and excretion of calcium and phosphorus.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaG2M%2Fltlamsg%3D%3D&md5=b94a91b93ea30b2912567e2bc00ccd03CAS | 14351662PubMed |

Moore JH, Tyler C (1955b) Studies on the intestinal absorption and excretion of calcium and phosphorus in the pig. 2. The intestinal absorption and excretion of radioactive calcium and phosphorus. British Journal of Nutrition 9, 81–93.
Studies on the intestinal absorption and excretion of calcium and phosphorus in the pig. 2. The intestinal absorption and excretion of radioactive calcium and phosphorus.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaG2M%2Fltlamsw%3D%3D&md5=fa502457f39299300a9bfcbcf2e691cbCAS | 14351663PubMed |

NRC (2012) ‘Nutrient requirements of swine.’ 11th rev. edn. (The National Academies Press: Washington, DC)

O’Brien J, Morrissey PA, Ames JM (1989) Nutritional and toxicological aspects of the Maillard browning reaction in foods. Critical Reviews in Food Science and Nutrition 28, 211–248.
Nutritional and toxicological aspects of the Maillard browning reaction in foods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXmtFegu7g%3D&md5=7a4737bd2802105b1d30967a12763199CAS | 2669832PubMed |

Ohta A, Ohtsuki M, Baba S, Takizawa T, Adachi T, Kimura S (1995) Effects of fructooligosaccharides on the absorption of iron, calcium and magnesium in iron-deficient rats. Journal of Nutritional Science and Vitaminology 41, 281–291.
Effects of fructooligosaccharides on the absorption of iron, calcium and magnesium in iron-deficient rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXms1Kit74%3D&md5=3cb76514effcfd09d32981fe85b1f243CAS | 7472673PubMed |

Partridge IG (1978) Studies on digestion and absorption in the intestines of growing pigs: 3. Net movements of mineral nutrients in the digestive tract. British Journal of Nutrition 39, 527–537.
Studies on digestion and absorption in the intestines of growing pigs: 3. Net movements of mineral nutrients in the digestive tract.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE1cXmtFeqsL0%3D&md5=1f15ef21c902b63b37140200181896ceCAS | 638122PubMed |

Patience JF, Gould SA, Koehler D, Corrigan B, Elsbernd A (2015) Super-dosed phytase improves rate and efficiency of gain in nursery pigs. Animal Industry Report AS661, ASL R3035. Iowa State University, Ames, IA, USA.

Qian H, Kornegay ET, Conner DE (1996) Adverse effects of wide calcium:phosphorus ratios on supplemental phytase efficacy for weanling pigs fed two dietary phosphorus levels. Journal of Animal Science 74, 1288–1297.

Rodríguez DA, Sulabo RC, González-Vega JC, Stein HH (2013) Energy concentration and phosphorus digestibility in canola, cottonseed, and sunflower products fed to growing pigs. Canadian Journal of Animal Science 93, 493–503.
Energy concentration and phosphorus digestibility in canola, cottonseed, and sunflower products fed to growing pigs.Crossref | GoogleScholarGoogle Scholar |

Rose DJ, DeMeo MR, Keshavarzian A, Hamaker BR (2007) Influence of dietary fiber on inflammatory bowel disease and colon cancer: importance of fermentation pattern. Nutrition Reviews 65, 51–62.
Influence of dietary fiber on inflammatory bowel disease and colon cancer: importance of fermentation pattern.Crossref | GoogleScholarGoogle Scholar | 17345958PubMed |

Ross RD, Cromwell GL, Stahly TS (1984) Effects of source and particle size on the biological availability of calcium in calcium supplements for growing pigs. Journal of Animal Science 59, 125–134.

Sandberg AS, Larsen T, Sandström B (1993) High dietary calcium level decreases colonic phytate degradation in pigs fed a rapeseed diet. The Journal of Nutrition 123, 559–566.

Scheideler SE (1998) Eggshell calcium effects on egg quality and Ca digestibility in first-or third-cycle laying hens. Journal of Applied Poultry Research 7, 69–74.
Eggshell calcium effects on egg quality and Ca digestibility in first-or third-cycle laying hens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXkt1Snu7s%3D&md5=f9784166f48514bdb6d1c39c647f870bCAS |

Schröder B, Breves G (2006) Mechanism and regulation of calcium absorption from the gastrointestinal tract in pigs and ruminants: comparative aspects with special emphasis on hypocalcemia in dairy cows. Animal Health Research Reviews 7, 31–41.
Mechanism and regulation of calcium absorption from the gastrointestinal tract in pigs and ruminants: comparative aspects with special emphasis on hypocalcemia in dairy cows.Crossref | GoogleScholarGoogle Scholar | 17389052PubMed |

Selle PH, Cowieson AJ, Ravindran V (2009) Consequences of calcium interactions with phytate and phytase for poultry and pigs. Livestock Science 124, 126–141.
Consequences of calcium interactions with phytate and phytase for poultry and pigs.Crossref | GoogleScholarGoogle Scholar |

Sims JT, Simard RR, Joern BC (1998) Phosphorus loss in agricultural drainage: historical perspective and current research. Journal of Environmental Quality 27, 277–293.
Phosphorus loss in agricultural drainage: historical perspective and current research.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXitFGhtrs%3D&md5=636090ff7b78ac1a4e46b382e646462eCAS |

Soares MJ, Murhadi LL, Kurpad AV, Chan She Ping-Delfos WL, Piers LS (2012) Mechanistic roles for calcium and vitamin D in the regulation of body weight. Obesity Reviews 13, 592–605.
Mechanistic roles for calcium and vitamin D in the regulation of body weight.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtlGrurjO&md5=5b2c3b28a42e2313113075893874f661CAS | 22385576PubMed |

Stein HH, Pedersen C, Wirt AR, Bohlke RA (2005) Additivity of values for apparent and standardized ileal digestibility of amino acids in mixed diets fed to growing pigs. Journal of Animal Science 83, 2387–2395.

Stein HH, Sève B, Fuller MF, Moughan PJ, de Lange CFM (2007) Invited review: amino acid bioavailability and digestibility in pig feed ingredients: terminology and application. Journal of Animal Science 85, 172–180.
Invited review: amino acid bioavailability and digestibility in pig feed ingredients: terminology and application.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjsFaqsQ%3D%3D&md5=172e2450056abeab167fb05395eed55aCAS | 17179553PubMed |

Stein HH, Adeola O, Cromwell GL, Kim SW, Mahan DC, Miller PS (2011) Concentration of dietary calcium supplied by calcium carbonate does not affect the apparent total tract digestibility of calcium, but reduces digestibility of phosphorus by growing pigs. Journal of Animal Science 89, 2139–2144.
Concentration of dietary calcium supplied by calcium carbonate does not affect the apparent total tract digestibility of calcium, but reduces digestibility of phosphorus by growing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXoslCisL8%3D&md5=71ae96fce6f070a689f03ba968e1256aCAS | 21335534PubMed |

Sulabo RC, Stein HH (2013) Digestibility of phosphorus and calcium in meat and bone meal fed to growing pigs. Journal of Animal Science 91, 1285–1294.
Digestibility of phosphorus and calcium in meat and bone meal fed to growing pigs.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmsFKntbY%3D&md5=52f0c36c5c9baebd6c3f131eac060f29CAS | 23230116PubMed |

van Abel M, Hoenderop JGJ, van der Kemp AWCM, van Leeuwen JPTM, Bindels RJM (2003) Regulation of the epithelial Ca2+ channels in small intestine as studied by quantitative mRNA detection. American Journal of Physiology. Gastrointestinal and Liver Physiology 285, G78–G85.
Regulation of the epithelial Ca2+ channels in small intestine as studied by quantitative mRNA detection.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXls1Wms7w%3D&md5=73a1336eaf7b160b9425cf5bce252516CAS | 12620887PubMed |

Wargovich MJ, Eng VWS, Newmark HL (1984) Calcium inhibits the damaging and compensatory proliferation effects of fatty acids on mouse colon epithelium. Cancer Letters 23, 253–258.
Calcium inhibits the damaging and compensatory proliferation effects of fatty acids on mouse colon epithelium.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXlt12gsrc%3D&md5=67d0aee9d7fedd7ce3103051719b4143CAS | 6744249PubMed |

Wise A (1983) Dietary factors determining the biological activities of phytate. Nutrition Abstracts and Reviews 53, 791–806.

Wong JMW, de Souza R, Kendall CWC, Emam A, Jenkins DJA (2006) Colonic health: fermentation and short chain fatty acids. Journal of Clinical Gastroenterology 40, 235–243.
Colonic health: fermentation and short chain fatty acids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xjslelsbg%3D&md5=aa5c07ba3de1b0de1782621af496a658CAS |