Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Effect of zinc concentration and source on performance, tissue mineral status, activity of superoxide dismutase enzyme and lipid peroxidation of meat in broiler chickens

S. Kamran Azad A , F. Shariatmadari A B , M. A. Karimi Torshizi A and Hamed Ahmadi A
+ Author Affiliations
- Author Affiliations

A Department of Animal Science, Faculty of agriculture, Tarbiat Modares University, Tehran, 14115-336, Iran.

B Corresponding author. Email: shariatf@modares.ac.ir; shariat_madari@yahoo.com

Animal Production Science - https://doi.org/10.1071/AN15758
Submitted: 10 November 2015  Accepted: 24 April 2017   Published online: 23 June 2017

Abstract

The present experiment was conducted to investigate the effect of feeding different concentrations and the source of zinc (Zn) on the performance, tissue mineral status, superoxide dismutase (SOD) enzyme activity and meat quality in 0–4-week-old broiler chicks. Dietary treatments included the corn–soybean meal-based diet (control) and the basal diet supplemented with Zn at 20, 50 or 80 mg/kg, added as ZnSO4, Zn-methionine or Zn-enriched yeast. The results showed that birds fed Zn-supplemented diets had higher average weight gain and average feed intake than did birds fed the control diet (P < 0.01). At the end of the experiment, the Zn deposition in pancreas, liver and tibia increased (P < 0.01), regardless of the source, in response to increasing dietary Zn concentrations, whereas plasma Zn status was significantly increased by the highest Zn supplementation level. The main effect of Zn supplementation level was significant for the activities of Cu and/or Zn SOD in the liver and pancreas (P < 0.01). As broiler given 50 mg Zn had higher tissue SOD activity than did broilers fed the other treatment diets. Furthermore, Zn supplementation at up to 50 mg/kg significantly increased (P < 0.01) Zn accumulation and SOD activity and decreased lipid peroxidation in muscles around the femur bone. Results from the present study demonstrated that supplementation with 50 mg Zn may be sufficient for normal broiler growth to 28 days of age and the dietary inclusion of organic Zn could be utilised more effectively than that of inorganic sources.

Additional keywords: broiler chicks, enzyme activities, meat quality, growth performance.


References

Aksu T, Aksu Mİ, Yoruk MA, Karaoglu M (2011) Effects of organically-complexed minerals on meat quality in chickens. British Poultry Science 52, 558–563.
Effects of organically-complexed minerals on meat quality in chickens.CrossRef | 1:CAS:528:DC%2BC3MXhtlyhu7bO&md5=6786f7e281dcff43120842fa693c24abCAS |

Anilkumar C, Ramana JV, Ramaprasad J, Sudheer SD, Reddy PS, Shakeela S (2012) Dietary supplementation of zinc sulphate and zinc-methionine: changes in levels of mineral composition (copper, zinc, iron and manganese) in various organs of broilers. Journal of Animal Production Advances 2, 409–419.

Ao T, Pierce JL, Power R, Pescatore AJ, Cantor AH, Dawson KA, Ford MJ (2009) Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks. Poultry Science 88, 2171–2175.
Effects of feeding different forms of zinc and copper on the performance and tissue mineral content of chicks.CrossRef | 1:CAS:528:DC%2BD1MXht1OgsbnE&md5=de08f6c1e8c759e209265f590f7dde9dCAS |

Ao T, Pierce JL, Pescatore AJ, Cantor AH, Dawson KA, Ford MJ, Paul M (2011) Effects of feeding different concentration and forms of zinc on the performance and tissue mineral status of broiler chicks. British Poultry Science 52, 466–471.
Effects of feeding different concentration and forms of zinc on the performance and tissue mineral status of broiler chicks.CrossRef | 1:CAS:528:DC%2BC3MXht1Wmtr7J&md5=9b28f728379774c834e8799a1762d948CAS |

AOAC (2000) ‘Official methods of analysis.’ 17th edn. (Association of Official Analytical Chemists: Arlington, VA)

Attia YA, Abd Al-Hamid AE, Zeweil HS, Qota EM, Bovera F, Monastra G, Sahledom MD (2013) Effect of dietary amounts of inorganic and organic zinc on productive and physiological traits of White Pekin ducks. Animal 7, 895–900.
Effect of dietary amounts of inorganic and organic zinc on productive and physiological traits of White Pekin ducks.CrossRef | 1:CAS:528:DC%2BC3sXntFWmt7Y%3D&md5=144c934a4169f2ad47761e70589472c5CAS |

Bao YM, Choct M, Iji PA, Bruerton K (2007) Effect of organically complexed copper, iron, manganese, and zinc on broiler performance, mineral excretion, and accumulation in tissues. Journal of Applied Poultry Research 16, 448–455.
Effect of organically complexed copper, iron, manganese, and zinc on broiler performance, mineral excretion, and accumulation in tissues.CrossRef | 1:CAS:528:DC%2BD2sXhtFahtb%2FM&md5=4fdc59ddf68acbece8c1dea56be5c58aCAS |

Bao YM, Choct M, Iji PA, Bruerton K (2009) Optimal dietary inclusion of organically complexed zinc for broiler chickens. British Poultry Science 50, 95–102.
Optimal dietary inclusion of organically complexed zinc for broiler chickens.CrossRef | 1:CAS:528:DC%2BD1MXksVWqtb4%3D&md5=51e13236db22a2058c82d5334eba8da0CAS |

Botsoglou NA, Fletouris DJ, Papageoargiou GE, Vassiliopoulos VN, Mantis AJ, Trakatellis AG (1994) Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feedstuff sample. Journal of Agricultural and Food Chemistry 42, 1931–1937.
Rapid, sensitive, and specific thiobarbituric acid method for measuring lipid peroxidation in animal tissue, food and feedstuff sample.CrossRef | 1:CAS:528:DyaK2cXlsFKjtrc%3D&md5=a9cb30de4aef553e9b2211f3f78ebdbeCAS |

Bou R, Guardiola F, Barroeta AC, Codony R (2005) Effect of dietary fat sources and zinc and selenium supplements on the composition and consumer acceptability of chicken meat. Poultry Science 84, 1129–1140.
Effect of dietary fat sources and zinc and selenium supplements on the composition and consumer acceptability of chicken meat.CrossRef | 1:CAS:528:DC%2BD2MXmsVamtro%3D&md5=37181e3057dc6793daf9b3c8c9e7231fCAS |

Bun SD, Guo YM, Guo FC, Ji FJ, Cao H (2011) Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella. Poultry Science 90, 1220–1226.
Influence of organic zinc supplementation on the antioxidant status and immune responses of broilers challenged with Eimeria tenella.CrossRef | 1:CAS:528:DC%2BC3MXot1Ohs7Y%3D&md5=9abaa0ab04cd84b87c8ba41c64b667c6CAS |

Cao J, Henry PR, Guo R (2000) Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants. Journal of Animal Science 78, 2039–2054.
Chemical characteristics and relative bioavailability of supplemental organic zinc sources for poultry and ruminants.CrossRef | 1:CAS:528:DC%2BD3cXlvVChtrs%3D&md5=55f76f6fb794821c9169f3939350e41aCAS |

Cao J, Henry PR, Davis SR, Cousins RJ, Miles RD, Littell RC, Ammerman C (2002) Relative bioavailability of organic zinc sources based on tissue zinc and metallothionein in chicks fed conventional dietary zinc concentrations. Animal Feed Science and Technology 101, 161–170.
Relative bioavailability of organic zinc sources based on tissue zinc and metallothionein in chicks fed conventional dietary zinc concentrations.CrossRef | 1:CAS:528:DC%2BD38XnsVSjs7g%3D&md5=e1339b964699899e0867c9361c72cfa0CAS |

Chesters JK (1997) Zinc. In ‘Handbook of nutritionally essential mineral elements’. (Eds BL O’Dell, RA Sunde) pp. 185–217. (Marcel Dekker Inc.: New York)

Davis SR, McMahon RJ, Cousins RJ (1998) Metallothionein knockout and transgenic mice exhibit altered intestinal processing of zinc with uniform zinc-dependent zinc transporter-1 expression. The Journal of Nutrition 128, 825–831.

Day FA, Panemangalore M, Brady FO (1981) In vivo and ex vivo effects of copper on rat liver metallothionein. Proceedings of the Society for Experimental Biology and Medicine 168, 306–310.
In vivo and ex vivo effects of copper on rat liver metallothionein.CrossRef | 1:CAS:528:DyaL38XkvFGqug%3D%3D&md5=13138acd02bc5a814c13bcb69d4a4dd8CAS |

Fairweather-Tait SJ (1987) The concept of bioavailability as it relates to iron nutrition. Nutrition Research 7, 319–325.
The concept of bioavailability as it relates to iron nutrition.CrossRef | 1:CAS:528:DyaL2sXkt1aht7g%3D&md5=98bec9d47eb6cd3320a83c85f5dee35aCAS |

Feng J, Ma WQ, Niu HH, Wu XM, Wang Y, Feng J (2010) Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers. Biological Trace Element Research 133, 203–211.
Effects of zinc glycine chelate on growth, hematological, and immunological characteristics in broilers.CrossRef | 1:CAS:528:DC%2BC3cXhtlenu7k%3D&md5=f790d78c614fe1c76c43697177bd8b1aCAS |

Fischer PWF, Giroux A, L’Abbe MR (1981) The effect of dietary zinc on intestinal copper absorption. The American Journal of Clinical Nutrition 34, 1670–1675.

Grynpas MD, Pritzker KPH, Hancock RG (1987) Neutron activation of bulk and selected trace elements in bone using a low flux slowpoke reactor. Biological Trace Element Research 13, 333–344.
Neutron activation of bulk and selected trace elements in bone using a low flux slowpoke reactor.CrossRef | 1:CAS:528:DyaL1cXhvVOntg%3D%3D&md5=e73f1a308e5a02d7e9b2048b1bd0e3dcCAS |

Huang YL, Lu L, Luo XG, Liu B (2007) An optimal dietary zinc level of broiler chicks fed a corn–soybean meal diet. Poultry Science 86, 2582–2589.
An optimal dietary zinc level of broiler chicks fed a corn–soybean meal diet.CrossRef | 1:CAS:528:DC%2BD2sXhsVygsbbF&md5=0d0626501bd5c82fdc6f4e809df82e45CAS |

Huang YL, Lu L, Li SF, Luo XG, Liu B (2009) Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn–soybean meal diet. Journal of Animal Science 87, 2038–2046.
Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn–soybean meal diet.CrossRef | 1:CAS:528:DC%2BD1MXms1eisr4%3D&md5=f551d7ed6a3f9b225a59116e81187d6bCAS |

Hudson BP, Dozier WA, Wilson JL, Sander JE, Ward TL (2004) Reproductive performance and immune status of caged broiler breeder hens provided diets supplemented with either inorganic or organic sources of zinc from hatching to 65 wk of age. Journal of Applied Poultry Research 13, 349–359.
Reproductive performance and immune status of caged broiler breeder hens provided diets supplemented with either inorganic or organic sources of zinc from hatching to 65 wk of age.CrossRef | 1:CAS:528:DC%2BD2cXlsl2muro%3D&md5=a195a7b7d7767becc0c97d55f62e1ed3CAS |

Kamran Azad S, Shariatmadari F, Karimi Torshizi MA (2014) Production of zinc-enriched biomass of Saccharomyces cerevisiae. Journal of Elementology 19, 313–326.

Kopeć W, Jamroz D, Wiliczkiewicz A, Biazik E, Pudlo A, Hikawczuk T, Skiba T, Korzeniowska M (2013) Influence of different histidine sources and zinc supplementation of broiler diets on dipeptide content and antioxidant status of blood and meat. British Poultry Science 54, 454–465.
Influence of different histidine sources and zinc supplementation of broiler diets on dipeptide content and antioxidant status of blood and meat.CrossRef |

Lai C, Huang W, Askari A, Klevay LM, Chiu TH (1995) Expression of glutathione peroxidase and catalase in copper-deficient rat liver and heart. The Journal of Nutritional Biochemistry 6, 256–262.
Expression of glutathione peroxidase and catalase in copper-deficient rat liver and heart.CrossRef | 1:CAS:528:DyaK2MXmtVehtbg%3D&md5=5ccfc5c0130135a318c1a88a7e7db37fCAS |

Leeson S (2005) Trace element requirements of poultry: validity of the NRC requirements. In ‘Redefining mineral nutrition’. (Eds JA Taylor-Pickerd, LA Tucker) pp. 107–118. (Nottingham University Press: Nottingham, UK)

Li S, Lu L, Hao S, Wang Y, Zhang L, Liu S, Liu B, Li K, Luo X (2011) Dietary manganese modulates expression of the manganese-containing superoxide dismutase gene in chickens. The Journal of Nutrition 141, 189–194.
Dietary manganese modulates expression of the manganese-containing superoxide dismutase gene in chickens.CrossRef | 1:CAS:528:DC%2BC3MXhsVemuro%3D&md5=960cfd742d7708c18c16a924ea7acb01CAS |

Littell RC, Henry PR, Lewis AJ, Ammerman CB (1997) Estimate of relative bioavailability of nutrients using SAS procedures. Journal of Animal Science 75, 2672–2683.
Estimate of relative bioavailability of nutrients using SAS procedures.CrossRef | 1:CAS:528:DyaK2sXmsVCntrY%3D&md5=de5a925d0cf8bad7f1ed7e55cc08b152CAS |

Loveridge N (1993) Micronutrients and longitudinal growth. The Proceedings of the Nutrition Society 52, 49–55.
Micronutrients and longitudinal growth.CrossRef | 1:STN:280:DyaK3s3mt1SntA%3D%3D&md5=2fe10e795f5e9ced339f87ce22a3c4d2CAS |

MacDonald RS (2000) The role of zinc in growth and cell proliferation. The Journal of Nutrition 130, 1500S–1508S.

Mohanna C, Nys Y (1999) Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens. British Poultry Science 40, 108–114.
Effect of dietary zinc content and sources on the growth, body zinc deposition and retention, zinc excretion and immune response in chickens.CrossRef | 1:CAS:528:DyaK1MXivFSks78%3D&md5=df052fdb812fea5df299f81b9a89ad1bCAS |

Noor R, Mittal S, Iqbal J (2002) Superoxide dismutate: applications and relevance to human disease. Medical Science Monitor 8, RA210–RA215.

NRC (1994) ‘Nutrient requirements of poultry.’ 9th revised edn. (National Academy Press: Washington, DC)

O’Dell BL, Savage JE (1957) Symptoms of zinc deficiency in the chick. Proceedings Fed Society 16, 394 [Abstract]

Petrovič V, Marcincak S, Popelka P, Nollet L, Kovac G (2009) Effect of dietary supplementation of trace elements on the lipid peroxidation in broiler meat assessed after a refrigerated and frozen storage. Journal of Animal and Feed Sciences 18, 499–507.
Effect of dietary supplementation of trace elements on the lipid peroxidation in broiler meat assessed after a refrigerated and frozen storage.CrossRef |

Pimentel JL, Cook ME, Greger JL (1991) Research note: bioavailability of zinc-methionine for chicks. Poultry Science 70, 1637–1639.
Research note: bioavailability of zinc-methionine for chicks.CrossRef |

Powell SR (2000) The antioxidant properties of zinc. The Journal of Nutrition 130, 1447–1454.

Rayman MP (2004) The use of high-selenium yeast to raise selenium status: how does it measure up? British Journal of Nutrition 92, 557–573.
The use of high-selenium yeast to raise selenium status: how does it measure up?CrossRef | 1:CAS:528:DC%2BD2cXovFCqs7o%3D&md5=81106adafaa017fcabfe5e5d8a44f3caCAS |

Rojas LX, McDowell LR, Cousins RJ, Martin FG, Wilkinson NS, Johnson AB, Velasquez JB (1995) Relative bioavailability of two organic and two inorganic zinc sources fed to sheep. Journal of Animal Science 73, 1202–1207.
Relative bioavailability of two organic and two inorganic zinc sources fed to sheep.CrossRef | 1:CAS:528:DyaK2MXkvV2gtbc%3D&md5=5927e4ae1106debfed3e4680c5139952CAS |

Sandoval M, Henry PR, Luo XG, Littell RC, Miles RD, Ammerman CB (1998) Performance and tissue zinc and metallothionein accumulation in chicks fed a high dietary level of zinc. Poultry Science 77, 1354–1363.
Performance and tissue zinc and metallothionein accumulation in chicks fed a high dietary level of zinc.CrossRef | 1:CAS:528:DyaK1MXmsFyr&md5=06b2dc86e6d84b65b81df4a07db52e3aCAS |

SAS Institute Inc (2001) ‘Statistical analytical system user’s guide.’ Version 8.00 edition. (SAS Institute Inc.: Cary, NC)

Shyam Sunder G, Panda NC, Gopinath SV, Rama Rao MVL, Raju MR, Reddy TM, Vijay Kumar C (2008) Effects of higher levels of zinc supplementation on performance, mineral availability, and immune competence in broiler chickens. Journal of Applied Poultry Research 17, 79–86.
Effects of higher levels of zinc supplementation on performance, mineral availability, and immune competence in broiler chickens.CrossRef |

Underwood EJ, Suttle N (2001) ‘The mineral nutrition of livestock.’ (CABI Publishing: London)

Vallee BL, Falchuk KH (1993) The biochemical basis of zinc physiology. Physiological Review 73, 79–118.

Wang F, Lu L, Li S, Liu S, Zhang L, Yao J, Luo X (2012) Relative bioavailability of manganese proteinate for broilers fed a conventional corn–soybean meal diet. Biological Trace Element Research 146, 181–186.
Relative bioavailability of manganese proteinate for broilers fed a conventional corn–soybean meal diet.CrossRef | 1:CAS:528:DC%2BC38XksVeqsb8%3D&md5=ada9da78ba916e69caaf40a89a942312CAS |

Wedekind KJ, Hortin AE, Baker DH (1992) Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide. Journal of Animal Science 70, 178–187.
Methodology for assessing zinc bioavailability: efficacy estimates for zinc-methionine, zinc sulfate, and zinc oxide.CrossRef | 1:CAS:528:DyaK38XitFegtbs%3D&md5=9428215ed6a2f0745643ace2e6827addCAS |

Yamaguchy S (1991) The role of SOD antioxidant. Journal of the National Cancer Institute 28, 221–232.

Yuan J, Xu Z, Huang C, Zhou S, Guo Y (2011) Effect of dietary Mintrex-Zn/Mn on performance, gene expression of Zn transfer proteins, activities of Zn/Mn related enzymes and fecal mineral excretion in broiler chickens. Animal Feed Science and Technology 168, 72–79.
Effect of dietary Mintrex-Zn/Mn on performance, gene expression of Zn transfer proteins, activities of Zn/Mn related enzymes and fecal mineral excretion in broiler chickens.CrossRef | 1:CAS:528:DC%2BC3MXpslWqsbo%3D&md5=55925df19b4d7b146eaf4be3270bb4acCAS |



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