Effect of the acute heat stress on serum endotoxin concentration and the expression of TLR4 mRNA in liver of Arbor Acres broiler chickensShu-cheng Huang A B , Ya-Fen Fu A , Mujeeb Ur Rehman B , Kun Li B , Yan-fang Lan B , Wei Liu A , Hou-qiang Luo B , Hui Zhang B , Li-hong Zhang B , Ya-nan Hao B and Zong-xi Tong A C
A College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
B College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
C Corresponding author. Email: email@example.com
Animal Production Science - https://doi.org/10.1071/AN16423
Submitted: 2 July 2016 Accepted: 23 July 2017 Published online: 21 February 2018
This study was undertaken to investigate the relationship between gut-derived endotoxin and the Toll-like receptor 4 (TLR4) expression in the liver of broilers under acute heat stress (AHS). For this purpose, 120 Arbor Acres chicks were randomised into two groups: control temperature group (CT group, 22 ± 1°C) and high temperature group (HT group, 38 ± 1°C). The chicks received AHS at Day 28 and their small intestine, liver and blood samples were collected after 2 h, 5 h and 10 h to examine the histopathology, biochemical parameters, endotoxin concentrations and TLR4 expression. The results showed that damaged intestinal villi and severe congestion of the hepatic sinusoids were observed, especially after 10 h of AHS in the HT group. In addition, the levels of alanine transferase, aspartate transaminase, and direct bilirubin, except alkaline phosphataseafter were significantly elevated (P < 0.05) and total bilirubin (P < 0.01) and albumin (P < 0.05) were decreased after 10 h of AHS as compared with the CT group, which are associated with liver function. Moreover, the mRNA expression of TLR4 in the liver was noticeably upregulated (P < 0.05) during AHS with significantly increased in endotoxin concentration (P < 0.01) of broilers. Altogether, these findings suggest that the upregulated expression of TLR4 mRNA was triggered via gut-derived endotoxin in heat stress-induced liver injury.
Additional keyword: liver injury.
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