Administration of a live attenuated Salmonella vaccine using an inactivated oil-emulsion vaccine as a vehicle for commercial chicken flocksP. J. Groves A C , T. Harris B and S. M. Sharpe B
A Poultry Research Foundation, School of Veterinary Science, Faculty of Science, The University of Sydney, 425 Werombi Road, Camden, NSW 2570, Australia.
B Birling Avian Laboratories, 975 The Northern Road, Bringelly, NSW 2556, Australia.
C Corresponding author. Email: email@example.com
Animal Production Science - https://doi.org/10.1071/AN16521
Submitted: 30 July 2016 Accepted: 25 November 2016 Published online: 11 April 2017
Since the finding that inoculating an aroA- deletion live Salmonella Typhimurium vaccine parenterally provides improved and longer-lasting protection against Salmonella colonisation of the laying-hen intestine, this administration route has been adopted by the industry. To make this method practicable and economical, mixing the live bacterial vaccine with an inactivated viral vaccine has become popular. In vitro and in vivo studies were performed designed to assess the effect on the survival of the live salmonellae and the ability to stimulate serum antibody when mixed into oil-emulsion vaccines, compared with more traditional diluents. A rapid decline in viable salmonellae was observed when mixing with an inactivated Riemerella/Pasteurella bacterin. Mixing with an inactivated viral vaccine produced a less severe and more gradual decline in viable salmonellae over time; however, there was a surprising resuscitation of the bacteria 60 min after mixing. Serum antibody 14 days after inoculation of vaccine diluted in a universal diluent rose significantly, compared with sham vaccinated birds. Birds receiving the vaccine diluted in an inactivated vaccine at the time of preparation did not show a significant serological response; however, when given 60 min post-preparation, serum antibody was significantly increased. There appeared to be a correlation of the magnitude of serum antibody produced with the number of viable salmonellae inoculated. The use of the live vaccine incorporated into an inactivated vaccine may give variable results and needs assessment before adoption.
Additional keywords: poultry, vaccine combination, vaccine vehicle.
ReferencesAbs El-Osta Y, Mohotti S, Carter F, Kuchipudi S, Youil R, Underwood G, Scott PC, Morrow CJ (2015) Preliminary analysis of the duration of protection of Vaxsafe® ST vaccine against Salmonella shedding in layers. In ‘Proceedings of the 26th Australian poultry science symposium’. (Ed. P Selle) pp. 163–165. (The University of Sydney: Sydney)
Babu U, Dalloul RA, Okamura M, Lillehoj HS, Xie H, Raybourne RB, Gaines D, Heckert RA (2004) Salmonella enteritidis clearance and immune responses in chickens following Salmonella vaccination and challenge. Veterinary Immunology and Immunopathology 101, 251–257.
| Salmonella enteritidis clearance and immune responses in chickens following Salmonella vaccination and challenge.CrossRef | 1:CAS:528:DC%2BD2cXntlGls7g%3D&md5=caaab7e999f1b8068446ebb8786246ddCAS |
Bailey JS, Rolón A, Hofacre CL, Holt PS, Wilson JL, Cosby DE, Richardson LJH, Cox NA (2007) Resistance to challenge of breeders and their progeny with and without competitive exclusion treatment to Salmonella vaccination programs in broiler breeders. International Journal of Poultry Science 6, 386–392.
| Resistance to challenge of breeders and their progeny with and without competitive exclusion treatment to Salmonella vaccination programs in broiler breeders.CrossRef |
Barrow PA (2007) Salmonella infections: immune and non-immune protection with vaccines. Avian Pathology 36, 1–13.
| Salmonella infections: immune and non-immune protection with vaccines.CrossRef | 1:CAS:528:DC%2BD2sXjsVOlsbs%3D&md5=90adc178ac3d4fc09b20f3907e511c46CAS |
Berghaus RD, Thayer SG, Maurer JJ, Hofacre CL (2011) Effect of vaccinating breeder chickens with a killed Salmonella vaccine on Salmonella prevalences and loads in breeder and broiler chicken flocks. Journal of Food Protection 74, 727–734.
| Effect of vaccinating breeder chickens with a killed Salmonella vaccine on Salmonella prevalences and loads in breeder and broiler chicken flocks.CrossRef | 1:STN:280:DC%2BC3Mvpt1Ciuw%3D%3D&md5=5f91671732fbaa6921a65749090db339CAS |
Davies R, Breslin M (2003) Effects of vaccination and other preventative method for Salmonella Enteritidis on commercial laying chicken farms. The Veterinary Record 153, 673–677.
| Effects of vaccination and other preventative method for Salmonella Enteritidis on commercial laying chicken farms.CrossRef | 1:STN:280:DC%2BD3sngtVKnug%3D%3D&md5=db34376298cb852b7f72dad2975958b1CAS |
Groves PJ, Sharpe SM, Cox JM (2015) Response of layer and broiler strain chickens to parenteral administration of a live Salmonella Typhimurium vaccine. Poultry Science 94, 1512–1520.
| Response of layer and broiler strain chickens to parenteral administration of a live Salmonella Typhimurium vaccine.CrossRef | 1:CAS:528:DC%2BC28XhvFektrnI&md5=edd465fc3b5462c8e633af0bca0ebe82CAS |
Groves PJ, Sharpe SM, Muir WI, Pavic A, Cox JM (2016) Live and inactivated vaccine regimes against caecal Salmonella Typhimurium colonisation in laying hens. Australian Veterinary Journal 94, 387–393.
| Live and inactivated vaccine regimes against caecal Salmonella Typhimurium colonisation in laying hens.CrossRef | 1:CAS:528:DC%2BC28XhsFKjsr3P&md5=1117b45f6029cc70e4e40971d1ada7acCAS |
Jackson CAW (2012) The safety of parenterally administered live deletion mutant bacterial vaccines for poultry. World’s poultry conference, 5–9 August, Salvador, Bahia, Brazil. PB_2012pc287_1.
MSD Animal Health (2015) Nobilis CAV P4 product details. Available at http://www.msd-animal-health.ph/products/131_118562/productdetails_131_118658.aspx [Verified 29 June 2016]
Okamura M, Lillehoj HS, Raybourne RB, Babu US, Heckert RA (2004) Cell-mediated immune responses to a killed Salmonella Enteritidis vaccine: lymphocyte proliferation, T-cell changes and interleukin-6 (IL-6), IL-1, IL-2, and IFB-γ production. Comparative Immunology, Microbiology and Infectious Diseases 27, 255–272.
| Cell-mediated immune responses to a killed Salmonella Enteritidis vaccine: lymphocyte proliferation, T-cell changes and interleukin-6 (IL-6), IL-1, IL-2, and IFB-γ production.CrossRef | 1:STN:280:DC%2BD2c3nvVWjuw%3D%3D&md5=d8a90f14e67a2d12811a7294943dad73CAS |
Pavic A, Groves PJ, Cox JM (2012) Control of Salmonella in poultry through vaccination and prophylactic antibody treatment. In ‘Biochemical testing’. (Ed. JC Jimenez-Lopez) pp. 86–126. (InTech: Rijeka, Croatia)
Sharpe SM, Groves PJ, Cox JM (2012) Serum antibody responses following Salmonella vaccination and challenge with Salmonella Typhimurium. In ‘Proceedings of the 23rd Australian poultry science symposium’. (Ed. JR Roberts) pp. 172–175. (The University of Sydney: Sydney)
Xie H, Rath NC, Huff GR, Huff WE, Balog JM (2000) Effects of Salmonella Typhimurium lipopolysaccharide on broiler chickens. Poultry Science 79, 33–40.
| Effects of Salmonella Typhimurium lipopolysaccharide on broiler chickens.CrossRef | 1:CAS:528:DC%2BD3cXoslSrtA%3D%3D&md5=ec62cfa70d7cf8f55fee2498a1a8f417CAS |