Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Growth and reproductive traits of F1-generation transgenic goats for human granulocyte-colony stimulating factor

R. I. T. P. Batista A C , J. M. G. Souza-Fabjan A B , D. Í. A. Teixeira A , L. M. Melo A and V. J. F. Freitas A
+ Author Affiliations
- Author Affiliations

A Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, Ceará State University, Av. Dr Silas Munguba, 1700 – 60714-903, Fortaleza-CE, Brazil.

B Faculty of Veterinary Medicine, School of Health Sciences, Grande Rio University, Rua Prof. José de Souza Herdy, 1160 – 25071-202, Duque de Caxias-RJ, Brazil.

C Corresponding author. Email: ribrio@yahoo.com.br

Animal Production Science - https://doi.org/10.1071/AN16582
Submitted: 27 August 2016  Accepted: 19 December 2016   Published online: 8 March 2017

Abstract

To ensure that animal welfare requirements and phenotypic characteristics of the newly produced transgenic lines are not compromised, an evaluation of all individuals is necessary. This can be inferred by the analysis of the growth and reproduction parameters. The present study was designed to determine the impact of the insertion of human granulocyte-colony stimulating factor (hG-CSF) transgene on growth and reproductive characteristics in first-generation (F1) goats from two transgenic lines. Bodyweight (BW) development (BW at birth, mean BW gain before weaning, BW at weaning, mean BW gain after weaning, BW at puberty), as well as reproductive parameters (age at puberty, ejaculate volume, concentration, total sperm per ejaculate, massal motility, progressive individual motility, major and minor defects) were similar (P > 0.05) between transgenic (T) and non-transgenic (NT) goats. Significant (P < 0.05) differences in mean (±s.d.) white blood cell count were observed between T and NT in first day of life (174.6 ± 14.7 × 103 and 15.0 ± 4.0 × 103 cells/µL), and during (66.8 ± 21.1 × 103 and 17.0 ± 4.6 × 103 cells/µL) and after (36.6 ± 4.0 × 103 and 15.5 ± 2.2 × 103 cells/µL) suckling, even though hG-CSF has not been detected in blood serum in any analysis. Although other cell counts were occasionally higher in T animals, differential counts showed that this difference was mainly due to an increased number of neutrophils, which represents 84.6%, 67.2% and 56.8% of total white blood cell count respectively, in the three time periods. Kidney and liver biochemical analyses indicated that all goats were healthy. Thus, it is possible to assume that all animals are normal and had no deleterious effects on either growth or reproductive parameters by the presence of transgene or as a consequence of leukocyte profile alteration.

Additional keywords: neutrophilia, puberty, welfare, white blood cell.


References

Al-Hozab A, Basiouni G (1999) Onset of puberty in Hebsi Zomri goats as monitored by plasma progesterone concentrations. Journal of Applied Animal Research 15, 69–74.
Onset of puberty in Hebsi Zomri goats as monitored by plasma progesterone concentrations.CrossRef |

ASAB (2006) Guidelines for the treatment of animals in behavioural research and teaching. Animal Behaviour 71, 245–253.
Guidelines for the treatment of animals in behavioural research and teaching.CrossRef |

Batista RITP, Melo CHS, Souza-Fabjan JMG, Teixeira DIA, Melo LM, Freitas VJF (2014) Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk. Biotechnology Letters 36, 2155–2162.
Phenotypic features of first-generation transgenic goats for human granulocyte-colony stimulation factor production in milk.CrossRef | 1:CAS:528:DC%2BC2cXhtVKrs7vM&md5=75a6051817d00535dcf9e21bc6f1394aCAS |

Boyd JW (1984) The interpretation of serum biochemistry test results in domestic animals. Veterinary Clinical Pathology 13, 7–14.
The interpretation of serum biochemistry test results in domestic animals.CrossRef | 1:STN:280:DC%2BD2cvgs12itg%3D%3D&md5=edee0a229bd58da57e2dba8ede38af04CAS |

Bryła M, Tr Zcińska M, Wieczorek J, Słomski R, Smorąg Z (2010) Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos. Animal Reproduction Science 118, 77–82.
Effect of semen quality in transgenic boars on the developmental competence of preimplantation embryos.CrossRef |

Chandler JE, Painter CL, Adkison RW, Memon MA, Hoyt PG (1988) Semen quality characteristics of dairy goats. Journal of Dairy Science 71, 1638–1646.
Semen quality characteristics of dairy goats.CrossRef | 1:STN:280:DyaL1c3pvFalug%3D%3D&md5=18bac0fe499b583ef7913ff5b15a5114CAS |

Crea F, Giovannetti E, Zinzanic PL, Danesi R (2009) Pharmacologic rationale for early G-CSF prophylaxis in cancer patients and role of pharmacogenetics in treatment optimization. Critical Reviews in Oncology/Hematology 72, 21–44.
Pharmacologic rationale for early G-CSF prophylaxis in cancer patients and role of pharmacogenetics in treatment optimization.CrossRef |

De Larco JE, Wuertz BR, Furcht LT (2004) The potential role of neutrophils in promoting the metastatic phenotype of tumors releasing Interleukin-8. Clinical Cancer Research 10, 4895–4900.
The potential role of neutrophils in promoting the metastatic phenotype of tumors releasing Interleukin-8.CrossRef | 1:CAS:528:DC%2BD2cXmtlSisbs%3D&md5=a124e1e3c5ba77d3d38de5490f47900bCAS |

Delgadillo JA, De Santiago-Miramontes MA, Carrillo E (2007) Season of birth modifies puberty in female and male goats raised under subtropical conditions. Animal 1, 858–864.
Season of birth modifies puberty in female and male goats raised under subtropical conditions.CrossRef | 1:STN:280:DC%2BC38vpt1Shsg%3D%3D&md5=4a397f2ca19268a61336777ea6ff86ddCAS |

Duncan JJ (2005) Science-based assessment of animal welfare: farm animals. Revue Scientifique et Technique 24, 483–492.
Science-based assessment of animal welfare: farm animals.CrossRef | 1:STN:280:DC%2BD2MnntVOqtA%3D%3D&md5=a5e6839868c36d809a00c1ed5d9bbb96CAS |

Freitas VJF, Serova IA, Andreeva LE, Dvoryanchikov GA, Lopes ES, Teixeira DIA, Dias LP, Avelar SRG, Moura RR, Melo LM, Pereira AF, Cajazeiras JB, Andrade ML, Almeida KC, Sousa FC, Carvalho AC, Serov OL (2007) Production of transgenic goat (Capra hircus) with human granulocyte colony stimulating factor (hG-CSF) gene in Brazil. Anais da Academia Brasileira de Ciencias 79, 585–592.
Production of transgenic goat (Capra hircus) with human granulocyte colony stimulating factor (hG-CSF) gene in Brazil.CrossRef | 1:CAS:528:DC%2BD1cXht1ejsb4%3D&md5=6b6b48d0c399c05f5d80020003f2b2a1CAS |

Freitas VJF, Serova IA, Moura RR, Andreeva LE, Melo LM, Teixeira DIA, Pereira AF, Lopes-Jr ES, Dias LPB, Nunes-Pinheiro DCS, Sousa FC, Alcântara-Neto AS, Albuquerque ES, Melo CHS, Rodrigues VHV, Batista RITP, Dvoryanchikov GA, Serov OL (2012) The establishment of two transgenic goat lines for mammary gland hG-CSF expression. Small Ruminant Research 105, 105–113.
The establishment of two transgenic goat lines for mammary gland hG-CSF expression.CrossRef |

Jackson KA, Berg JM, Murray JD, Maga EA (2010) Evaluating the fitness of human lysozyme transgenic dairy goats: growth and reproductive traits. Transgenic Research 19, 977–986.
Evaluating the fitness of human lysozyme transgenic dairy goats: growth and reproductive traits.CrossRef | 1:CAS:528:DC%2BC3cXhtlKmu7%2FJ&md5=af169104b54e15a8b35bb2702a3c449bCAS |

Jacobs L, Nawrot TS, de Geus B, Meeusen R, Degraeuwe B, Bernard A, Sughis M, Nemery B, Panis LI (2010) Subclinical responses in healthy cyclists briefly exposed to traffic-related air pollution. Environmental Health 9, 64
Subclinical responses in healthy cyclists briefly exposed to traffic-related air pollution.CrossRef |

Kaneko JJ, Harvey JW, Bruss ML (1997) Clinical biochemistry of domestic animals.’ 5th edn. (Academic Press: San Diego, CA)

Lavine G (2009) FDA approves first biological product derived from transgenic animal. American Journal of Health-System Pharmacy 66, 518
FDA approves first biological product derived from transgenic animal.CrossRef |

Maleszewski M, Kuretake S, Evenson D, Yanagimachi H, Bjordahl J, Yanagimachi R (1998) Behavior of transgenic mouse spermatozoa with galline protamine. Biology of Reproduction 58, 8–14.
Behavior of transgenic mouse spermatozoa with galline protamine.CrossRef | 1:CAS:528:DyaK1cXhsl2isQ%3D%3D&md5=75e874267fb4926fb1a0f8b8b060610bCAS |

Melo CH, Sousa FC, Batista RIPT, Sanchez DJD, Souza-Fabjan JMG, Freitas VJF, Melo LM, Teixeira DIA (2015) Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats. Genetics and Molecular Research 14, 8672–8684.
Comparative analysis of laparoscopic and ultrasound-guided biopsy methods for gene expression analysis in transgenic goats.CrossRef | 1:CAS:528:DC%2BC28XktVKhurw%3D&md5=4d2154f118c58dfa72889841c6ce33afCAS |

Mertens C, Rulicke T (2007) Welfare assessment and phenotype characterization of transgenic mice. ALTEX 24, 46–48.

Moura RR, Albuquerque ES, Melo CH, Alcântara-Neto AS, Batista RITP, Nunes-Pinheiro DC, Pereira AF, Teixeira DI, Melo LM, Serova IA, Andreeva LE, Serov OL, Freitas VJ (2013) Dynamics of recombinant hG-CSF in transgenic goat: preliminary study in the founder during hormonally induced lactation. Animal Biotechnology 24, 10–14.
Dynamics of recombinant hG-CSF in transgenic goat: preliminary study in the founder during hormonally induced lactation.CrossRef |

Moura RR, Souza-Fabjan JMG, Fonseca JF, Melo CHS, Sanchez DJD, Vieira MP, Almeida TM, Serova IA, Serov OL, Pereira AF, Teixeira DIA, Melo LM, Freitas VJF (2014) Reproductive parameters and the use of MOET in transgenic founder goat carrying the human granulocyte colony-stimulating factor (hG-CSF) gene. Animal Reproduction 11, 37–43.

National Research Council (2007) ‘Nutrient requirements of small ruminants.’ (The National Academies Press: Washington, DC)

Nur Z, Dogan I, Gunay U, Kemal Soylu M (2005) Relationship between sperm membrane integrity and other semen quality characteristics of the semen of Saanen goat bucks. Bulletin of the Veterinary Institute in Pulawy 49, 183–187.

Pugh DG (2002) ‘Sheep and goat medicine.’ 1st edn. (W.B. Saunders Company: Philadelphia, PA)

Pursel VG, Hammer RE, Bolt DJ, Palmiter RD, Brinster RL (1990) Integration, expression and germ-line transmission of growth-related genes in pig. Journal of Reproduction and Fertility 41, 77–87.

Pursel VG, Mitchell AD, Bee G, Elsasser TH, McMurtry JP, Wall RJ, Coleman ME, Scwartz RJ (2004) Growth and tissue accretion rates of swine expressing an insulin-like growth factor I transgene. Animal Biotechnology 15, 33–45.
Growth and tissue accretion rates of swine expressing an insulin-like growth factor I transgene.CrossRef | 1:CAS:528:DC%2BD2cXksVSmsrk%3D&md5=627634760bad28cd63c4450502aa07d5CAS |

Richt JA, Kasinathan P, Hamir AN, Castilla J, Sathiyaseelan T, Vargas F, Sathiyaseelan J, Wu H, Matsushita H, Koster J, Kato S, Ishida I, Soto C, Robl JM, Kuroiwa Y (2007) Production of cattle lacking prion protein. Nature Biotechnology 25, 132–138.
Production of cattle lacking prion protein.CrossRef | 1:CAS:528:DC%2BD2sXis1GrsA%3D%3D&md5=6a5cda4bdd4178f3e8ef589088de0103CAS |

Sambrook J, Fritsch EF, Maniatis T (1989) ‘Molecular cloning: a laboratory manual.’ (Cold Spring Harbor Laboratory Press: New York)

Souza LEB, Cruz JF, Neto MRT, Nunes RCS, Cruz MHC (2011) Puberty and sexual maturity in Anglo-Nubian male goats raised in semiintensive system. Revista Brasileira de Zootecnia 40, 1533–1539.

Tesson L, Rémy S, Ménoret S, Usal C, Anegon I (2002) Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR. Transgenic Research 11, 43–48.
Rapid and accurate determination of zygosity in transgenic animals by real-time quantitative PCR.CrossRef | 1:CAS:528:DC%2BD38Xit1Cmtbc%3D&md5=3fb8a074031b89d0d203c2319203eda1CAS |

Van Reenen CG (2009) Assessing the welfare of transgenic farm animals. In ‘Genetic engineering in livestock, new applications and interdisciplinary perspectives’. (Eds EM Hagen, K M Boysen) pp. 119–143. (Springer: Berlin)

Waugh DJ, Wilson C (2008) The interleukin-8 pathway in cancer. Clinical Cancer Research 14, 6735–6741.
The interleukin-8 pathway in cancer.CrossRef | 1:CAS:528:DC%2BD1cXhtlens7vL&md5=486240e436c92d611d819967007a39f1CAS |

Wheeler MB (2003) Production of transgenic livestock: promise fulfilled. Journal of Animal Science 81, 32–37.
Production of transgenic livestock: promise fulfilled.CrossRef | 1:CAS:528:DC%2BD2cXjvVyhtrs%3D&md5=9bdcaa1773c8068e804be342ab999fbcCAS |



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