Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE (Open Access)

Feeding wet distillers grains plus solubles contributes to sarcoplasmic reticulum membrane instability

M. D. Chao A B , K. I. Domenech-Perez A , L. S. Senaratne-Lenagala A and C. R. Calkins A C
+ Author Affiliations
- Author Affiliations

A Department of Animal Science, University of Nebraska-Lincoln, Lincoln, NE 68583-0908, USA.

B Present address: College of Agriculture, California State University, Chico, CA 95929, USA.

C Corresponding author. Email: Ccalkins1@unl.edu

Animal Production Science - https://doi.org/10.1071/AN16784
Submitted: 1 December 2016  Accepted: 22 June 2017   Published online: 8 August 2017

Abstract

Feeding wet distillers grains plus solubles (WDGS) increases polyunsaturated fatty acid (PUFA) levels in beef. It was hypothesised that WDGS in feedlot diets increases PUFA concentration in the sarcoplasmic reticulum (SR) membrane, thereby altering membrane integrity, resulting in more rapid intracellular calcium leakage and improved tenderness. The objective of this study was to evaluate this hypothesis. Ninety-six crossbred steers were fed either a corn-based diet with 0% WDGS or 50% WDGS. Fifteen strip loins per treatment were collected, fabricated into steaks, aged and placed under retail display conditions. Steaks were used to measure tenderness, proteolysis, free calcium concentrations, lipid oxidation, sarcomere length and SR membrane fatty acid, phospholipid lipid, neutral lipid and total lipid profiles. Compared with steaks from steers fed 0% WDGS, steaks from steers fed 50% WDGS were more tender (P < 0.05) and had greater (P < 0.05) free calcium concentrations early post-mortem. Feeding 50% WDGS also tended to increase (P < 0.10) total PUFA concentrations, decrease (P < 0.10) total phospholipid concentration and increase (P < 0.10) total neutral lipid concentration for SR membrane. Steaks from steers fed 0% WDGS had greater (P < 0.05) lipid oxidation (TBARS values) than steaks from steers fed 50% WDGS after extended aging. Although differences in tenderness between the two treatments were detected, there were no corresponding differences (P > 0.10) in sarcomere length or proteolysis. This study showed that feeding WDGS may increase tenderness, possibly by increasing free calcium in muscle early post-mortem. However, the true mechanism that contributes to these differences is still unclear.

Additional keywords: beef, fatty acids, phospholipids.


References

Abramson JJ, Salama G (1989) Critical sulfhydryls regulate calcium release from sarcoplasmic reticulum. Journal of Bioenergetics and Biomembranes 21, 283–294.
Critical sulfhydryls regulate calcium release from sarcoplasmic reticulum.CrossRef | 1:CAS:528:DyaL1MXktlGitbY%3D&md5=77730b1d554edd6a2b5ab59f62c2eb24CAS |

Ahn DU, Olson DG, Jo C, Chen X, Wu C, Lee JI (1998) Effect of muscle type, packaging, and irradiation on lipid oxidation, volatile production, and color in raw pork patties. Meat Science 49, 27–39.
Effect of muscle type, packaging, and irradiation on lipid oxidation, volatile production, and color in raw pork patties.CrossRef | 1:CAS:528:DyaK1cXisFygtrg%3D&md5=830eacd7bfeb896597c988e5ef203239CAS |

Atmaca G (2004) Antioxidant effects of sulfur-containing amino acids. Yonsei Medical Journal 45, 776–788.
Antioxidant effects of sulfur-containing amino acids.CrossRef | 1:CAS:528:DC%2BD2cXhtVKitbvM&md5=aff207d0c1c3a2068c8c3c4448252914CAS |

Baron CB, Coburn RF (1984) Comparison of two copper reagents for detection of saturated and unsaturated neutral lipids by charring densitometry. Journal of Liquid Chromatography 7, 2793–2801.
Comparison of two copper reagents for detection of saturated and unsaturated neutral lipids by charring densitometry.CrossRef | 1:CAS:528:DyaL2MXlvVOgtQ%3D%3D&md5=329a4f26167bec02416fd317673e9d68CAS |

Battin EE, Brumaghim JL (2009) Antioxidant activity of sulfur and selenium, a review of reactive oxygen species scavenging, glutathione peroxidase, and metal-binding antioxidant mechanisms. Cell Biochemistry and Biophysics 55, 1–23.
Antioxidant activity of sulfur and selenium, a review of reactive oxygen species scavenging, glutathione peroxidase, and metal-binding antioxidant mechanisms.CrossRef | 1:CAS:528:DC%2BD1MXosV2ksL8%3D&md5=385269f05a5739e962f827b8c1c0a279CAS |

Beare JL, Kates M (1964) The deposition of linoleic acid in rats fed corn oil. Canadian Journal of Biochemistry 42, 1477–1486.
The deposition of linoleic acid in rats fed corn oil.CrossRef | 1:CAS:528:DyaF2cXkvFCltbw%3D&md5=c37ace9881b6d4178c6f5d8e721421faCAS |

Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37, 911–917.
A rapid method of total lipid extraction and purification.CrossRef | 1:CAS:528:DyaG1MXhtVSgt70%3D&md5=4e60a5c754d3f3b9f9701b07dd6a3f60CAS |

Boleman S, Boleman S, Morgan W, Hale D, Griffin D, Savell J, Ames R, Smith M, Tatum J, Field T (1998) National Beef Quality Audit-1995: survey of producer-related defects and carcass quality and quantity attributes. Journal of Animal Science 76, 96–103.
National Beef Quality Audit-1995: survey of producer-related defects and carcass quality and quantity attributes.CrossRef | 1:CAS:528:DyaK1cXlsVKjtw%3D%3D&md5=9d04dc551e97124be6fc90991deab3c3CAS |

Brasitus TA, Davidson NO, Schachter D (1985) Variations in dietary triacylglycerol saturation alter the lipid composition and fluidity of rat intestinal plasma membranes. BBA-Biomembranes 812, 460–472.
Variations in dietary triacylglycerol saturation alter the lipid composition and fluidity of rat intestinal plasma membranes.CrossRef | 1:CAS:528:DyaL2MXhtFajtb4%3D&md5=f36108dfe40110a1a4c3ce67e838f14dCAS |

Cheah AM (1981) Effect of long chain unsaturated fatty acids on the calcium transport of sarcoplasmic reticulum. BBA-Biomembranes 648, 113–119.
Effect of long chain unsaturated fatty acids on the calcium transport of sarcoplasmic reticulum.CrossRef | 1:CAS:528:DyaL38Xit1yh&md5=fd63545f7771497aa7f073db28ab0bafCAS |

Chung KD, Lunt D, Kawachi H, Yano H, Smith S (2007) Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short-and long-fed Angus and Wagyu steers fed corn-or hay-based diets. Journal of Animal Science 85, 380–387.
Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short-and long-fed Angus and Wagyu steers fed corn-or hay-based diets.CrossRef | 1:CAS:528:DC%2BD2sXht1elsrY%3D&md5=7700494125f65b6858154955f1d59b53CAS |

Cross H, West R, Dutson T (1981) Comparison of methods for measuring sarcomere length in beef semitendinosus muscle. Meat Science 5, 261–266.
Comparison of methods for measuring sarcomere length in beef semitendinosus muscle.CrossRef | 1:STN:280:DC%2BC3MbmvFClsA%3D%3D&md5=f8f551c8f2ea61d908630f19b59ffccbCAS |

Dannenberger D, Nuernberg G, Scollan N, Ender K, Nuernberg K (2007) Diet alters the fatty acid composition of individual phospholipid classes in beef muscle. Journal of Agricultural and Food Chemistry 55, 452–460.
Diet alters the fatty acid composition of individual phospholipid classes in beef muscle.CrossRef | 1:CAS:528:DC%2BD28Xhtlaru7rF&md5=ee673b57d51469335a416046fbfb95f1CAS |

Deaver OE, Wander RC, McCusker R, Berdanier CD (1986) Diet effects on membrane phospholipid fatty acids and mitochondrial function in BHE rats. The Journal of Nutrition 116, 1148–1155.

Depenbusch BE, Coleman CM, Higgins JJ, Drouillard JS (2009) Effects of increasing levels of dried corn distillers grains with solubles on growth performance, carcass characteristics, and meat quality of yearling heifers. Journal of Animal Science 87, 2653–2663.
Effects of increasing levels of dried corn distillers grains with solubles on growth performance, carcass characteristics, and meat quality of yearling heifers.CrossRef | 1:CAS:528:DC%2BD1MXptV2gtL8%3D&md5=f01c2c415cba201bf0d407cf4370e2b8CAS |

Dolazza RM, Lorenzen CL (2014) Can samples be powdered to determine sarcomere length? Journal of Animal Science 92, 129–130.

Domenech K, Varnold KA, Semler ME, Chao M, Jones T, Erickson GE, Calkins CR (2014) Effects of feeding de-oiled wet distillers grains plus solubles on beef fatty acid profiles. Nebraska Beef Report MP99, 116–118.

Drewnoski M, Pogge D, Hansen S (2014) High-sulfur in beef cattle diets: a review. Journal of Animal Science 92, 3763–3780.
High-sulfur in beef cattle diets: a review.CrossRef | 1:CAS:528:DC%2BC2cXhslSjtbbK&md5=8c42aa02d4d44b3762dbde14512dab47CAS |

Garcia L, Nicholson K, Hoffman T, Lawrence T, Hale D, Griffin D, Savell J, VanOverbeke D, Morgan J, Belk K (2008) National Beef Quality Audit–2005: Survey of targeted cattle and carcass characteristics related to quality, quantity, and value of fed steers and heifers. Journal of Animal Science 86, 3533–3543.
National Beef Quality Audit–2005: Survey of targeted cattle and carcass characteristics related to quality, quantity, and value of fed steers and heifers.CrossRef | 1:CAS:528:DC%2BD1cXhsV2jsbrI&md5=bafc092a88c249de5983e91de04537a3CAS |

Gill C (1996) Extending the storage life of raw chilled meats. Meat Science 43, 99–109.
Extending the storage life of raw chilled meats.CrossRef |

Ham G, Stock R, Klopfenstein T, Larson E, Shain D, Huffman R (1994) Wet corn distillers byproducts compared with dried corn distillers grains with solubles as a source of protein and energy for ruminants. Journal of Animal Science 72, 3246–3257.

Hemmings SJ (2001) New methods for the isolation of skeletal muscle sarcolemma and sarcoplasmic reticulum allowing a comparison between the mammalian and amphibian beta(2)-adrenergic receptors and calcium pumps. Cell Biochemistry and Function 19, 133–141.
New methods for the isolation of skeletal muscle sarcolemma and sarcoplasmic reticulum allowing a comparison between the mammalian and amphibian beta(2)-adrenergic receptors and calcium pumps.CrossRef | 1:CAS:528:DC%2BD3MXktVSrtb4%3D&md5=33cf63000e53ba913601cbd8dd29f3b3CAS |

Hidalgo C, Bull R, Marengo JJ, Perez CF, Donoso P (2000) SH oxidation stimulates calcium release channels (ryanodine receptors) from excitable cells. Biological Research 33, 113–124.
SH oxidation stimulates calcium release channels (ryanodine receptors) from excitable cells.CrossRef | 1:CAS:528:DC%2BD3MXhtVamsbg%3D&md5=ff653df2ae3779ac940becb83e2e0290CAS |

Huang CH, Huang MC, Hou PC (1998) Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus x O. aureus. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology 120, 331–336.
Effect of dietary lipids on fatty acid composition and lipid peroxidation in sarcoplasmic reticulum of hybrid tilapia, Oreochromis niloticus x O. aureus.CrossRef |

Hunter GW, Negash S, Squier TC (1999) Phosphatidylethanolamine modulates Ca-ATPase function and dynamics. Biochemistry 38, 1356–1364.
Phosphatidylethanolamine modulates Ca-ATPase function and dynamics.CrossRef | 1:CAS:528:DyaK1MXhslOnuw%3D%3D&md5=69194a1ad5e5fca4e7e7d523a82edaa2CAS |

Hwang D, Hour J, Cheng H (2000) Effect of taurine on toxicity of oxidized fish oil in rats. Food and Chemical Toxicology 38, 585–591.
Effect of taurine on toxicity of oxidized fish oil in rats.CrossRef | 1:CAS:528:DC%2BD3cXlt1Giu7w%3D&md5=c80194155a89499a796a80f977cfaa57CAS |

Ji JR, Takahashi K (2006) Changes in concentration of sarcoplasmic free calcium during post-mortem ageing of meat. Meat Science 73, 395–403.
Changes in concentration of sarcoplasmic free calcium during post-mortem ageing of meat.CrossRef | 1:CAS:528:DC%2BD28XksF2jsLc%3D&md5=80da70fe80761b623c099e4ac01c74f7CAS |

Jolly ML, Nuttelman BL, Burken D, Schneider CJ, Klopfenstein TJ, Erickson GE (2014) Effects of increasing inclusion of wet distillers grains plus solubles with and without oil extraction on finishing performance. Nebraska Beef Report MP99, 81–82.

Kinman L, Hilton G, Richards C, Morgan J, Krehbiel C, Hicks R, Dillwith J, VanOverbeke D (2011) Impact of feeding various amounts of wet and dry distillers grains to yearling steers on palatability, fatty acid profile, and retail case life of longissimus muscle. Journal of Animal Science 89, 179–184.
Impact of feeding various amounts of wet and dry distillers grains to yearling steers on palatability, fatty acid profile, and retail case life of longissimus muscle.CrossRef | 1:CAS:528:DC%2BC3MXotl2ktA%3D%3D&md5=5f2e23d99365111c9a6f3555b6b6b493CAS |

Klopfenstein TJ, Erickson GE, Bremer VR (2008) Board-invited review: Use of distillers byproducts in the beef cattle feeding industry. Journal of Animal Science 86, 1223–1231.
Board-invited review: Use of distillers byproducts in the beef cattle feeding industry.CrossRef | 1:CAS:528:DC%2BD1cXltlWruro%3D&md5=9668678fd700d0f03804f3407f79c78aCAS |

Koger TJ, Wulf DM, Weaver AD, Wright CL, Tjardes KE, Mateo KS, Engle TE, Maddock RJ, Smart AJ (2010) Influence of feeding various quantities of wet and dry distillers grains to finishing steers on carcass characteristics, meat quality, retail-case life of ground beef, and fatty acid profile of longissimus muscle. Journal of Animal Science 88, 3399–3408.
Influence of feeding various quantities of wet and dry distillers grains to finishing steers on carcass characteristics, meat quality, retail-case life of ground beef, and fatty acid profile of longissimus muscle.CrossRef | 1:CAS:528:DC%2BC3cXhtF2rtb3O&md5=5a1f4cc6aaedde5f26204875af225b11CAS |

Larick DK, Turner BE (1990) Flavor characteristics of forage-fed and grain-fed beef as influenced by phospholipid and fatty acid compositional differences. Journal of Food Science 55, 312–317.
Flavor characteristics of forage-fed and grain-fed beef as influenced by phospholipid and fatty acid compositional differences.CrossRef | 1:CAS:528:DyaK3cXktl2jtbk%3D&md5=15268d6c9c25799c68dda07f2cde0fb1CAS |

Leray C, Pelletier X, Hemmendinger S, Cazenave JP (1987) Thin-layer chromatography of human platelet phospholipids with fatty acid analysis. Journal of Chromatography. A 420, 411–416.
Thin-layer chromatography of human platelet phospholipids with fatty acid analysis.CrossRef | 1:CAS:528:DyaL2sXlvFCgurk%3D&md5=32aaef7ffd22bc3c6144f92e7517065eCAS |

McKenna D, Roebert D, Bates P, Schmidt T, Hale D, Griffin D, Savell J, Brooks J, Morgan J, Montgomery T (2002) National Beef Quality Audit-2000: survey of targeted cattle and carcass characteristics related to quality, quantity, and value of fed steers and heifers. Journal of Animal Science 80, 1212–1222.
National Beef Quality Audit-2000: survey of targeted cattle and carcass characteristics related to quality, quantity, and value of fed steers and heifers.CrossRef | 1:CAS:528:DC%2BD38XmvVeksb0%3D&md5=f5e8638c06c5e2e9991f80dd905811a8CAS |

Mead JF, Stein RA, Wu GS, Sevanian A, Gan-Elepano M (1980) Peroxidation of lipids in model systems and in biomembranes. In ‘Autoxidation in food and biological systems’. (Eds MG Simic, M Karel) pp. 413–428. (Springer: New York)

Mello AS, Calkins CR, Jenschke BE, Carr TP, Dugan MER, Erickson GE (2012) Beef quality of calf-fed steers finished on varying levels of corn-based wet distillers grains plus solubles. Journal of Animal Science 90, 4625–4633.
Beef quality of calf-fed steers finished on varying levels of corn-based wet distillers grains plus solubles.CrossRef | 1:CAS:528:DC%2BC3sXns1SqtQ%3D%3D&md5=fd9e7ac20cc95e179c354ccff49c3421CAS |

Metcalfe L, Schmitz AA, Pelka J (1966) Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry 38, 514–515.
Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis.CrossRef | 1:CAS:528:DyaF28XosFKltA%3D%3D&md5=6a95b21233ca7a4bc6f6cd6906654dbdCAS |

Miller MF, Carr MA, Ramsey CB, Crockett CL, Hoover LC (2001) Consumer thresholds for establishing the value of beef tenderness. Journal of Animal Science 79, 3062–3068.
Consumer thresholds for establishing the value of beef tenderness.CrossRef | 1:CAS:528:DC%2BD38XivFCmtA%3D%3D&md5=bbcef837330f9c5959c29885b7173fe3CAS |

Miller RK, Smith SB, Carstens GE (2011) New Beef Tenderness Theory – Executive Summary. Prepared for the National Cattlemen’s Beef Association, Centenial, CO.

Mlekusch W, Celedin C, Aloia RC, Moller R (1993) Effect of a high fat diet on phospholipid class distribution and fatty acid composition in rat liver. The International Journal of Biochemistry 25, 1539–1547.
Effect of a high fat diet on phospholipid class distribution and fatty acid composition in rat liver.CrossRef | 1:CAS:528:DyaK2cXivF2mug%3D%3D&md5=a735b2e58af102e4e69e467ced7b988aCAS |

Morrison WR, Smith LM (1964) Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol. Journal of Lipid Research 5, 600–608.

Noci F, Monahan FJ, French P, Moloney AP (2005) The fatty acid composition of muscle fat and subcutaneous adipose tissue of pasture-fed beef heifers: Influence of the duration of grazing. Journal of Animal Science 83, 1167–1178.
The fatty acid composition of muscle fat and subcutaneous adipose tissue of pasture-fed beef heifers: Influence of the duration of grazing.CrossRef | 1:CAS:528:DC%2BD2MXjslShtLg%3D&md5=47f5231fa453acdfac4eba4ad46b5ae3CAS |

Nurnberg K, Kuchenmeister U, Ender K, Nurnberg G, Hackl W (1998) Influence of dietary n-3 fatty acids on the membrane properties of skeletal muscle in pigs. European Journal of Lipid Science and Technology 100, 353–358.

Oltra O, Farmer L, Moss B, Birnie J (2008) The effect of distiller’s and brewer’s grains on beef acceptability. Proceeding of International Congress of Meat science and Technology 54. Cape Town, South Africa.

Parcell S (2002) Sulfur in human nutrition and applications in medicine. Alternative Medicine Review 7, 22–44.

Parrish FC, Selvig CJ, Culler RD, Zeece MG (1981) CAF activity, calcium concentration, and the 30,000-dalton component of tough and tender bovine longissimus muscle. Journal of Food Science 46, 308–311.
CAF activity, calcium concentration, and the 30,000-dalton component of tough and tender bovine longissimus muscle.CrossRef | 1:CAS:528:DyaL3MXot1Cisw%3D%3D&md5=c94c1331ff8ba81f2c8cda1ce4d4e26aCAS |

Pearson AW, Carse WA, Davey CL, Locker RH, Hagyard CJ, Kirton AH (1973) Influence of epinephrine and calcium upon glycolysis, tenderness and shortening of sheep muscle. Journal of Food Science 38, 1124–1127.
Influence of epinephrine and calcium upon glycolysis, tenderness and shortening of sheep muscle.CrossRef |

Pietrzak M, Greaser ML, Sosnicki AA (1997) Effect of rapid rigor mortis processes on protein functionality in pectoralis major muscle of domestic turkeys. Journal of Animal Science 75, 2106–2116.
Effect of rapid rigor mortis processes on protein functionality in pectoralis major muscle of domestic turkeys.CrossRef | 1:CAS:528:DyaK2sXltl2itLk%3D&md5=04785c5ec36c27543aa4ccac135f643fCAS |

Platter WJ, Tatum JD, Belk KE, Koontz SR, Chapman PL, Smith GC (2005) Effects of marbling and shear force on consumers’ willingness to pay for beef strip loin steaks. Journal of Animal Science 83, 890–899.
Effects of marbling and shear force on consumers’ willingness to pay for beef strip loin steaks.CrossRef | 1:CAS:528:DC%2BD2MXivVCqsLY%3D&md5=fbd5cb47c4f6dda209930f53362940dcCAS |

Roeber D, Gill R, DiCostanzo A (2005) Meat quality responses to feeding distiller’s grains to finishing Holstein steers. Journal of Animal Science 83, 2455–2460.
Meat quality responses to feeding distiller’s grains to finishing Holstein steers.CrossRef | 1:CAS:528:DC%2BD2MXhtVKqsLnO&md5=320421d889c8a07a967b7e34f0374a41CAS |

Segers J, Stewart R, Lents C, Pringle T, Froetschel M, Lowe B, McKeith R, Stelzleni A (2011) Effect of long-term corn by-product feeding on beef quality, strip loin fatty acid profiles, and shelf life. Journal of Animal Science 89, 3792–3802.
Effect of long-term corn by-product feeding on beef quality, strip loin fatty acid profiles, and shelf life.CrossRef | 1:CAS:528:DC%2BC3MXhsVSqsrjP&md5=7ab5cf5c074afd881ea59e7a7a348b35CAS |

Senaratne LS (2012) Mechanism and control of beef Toughening during retail display in high oxygen modified atmosphere packages. PhD Thesis, University of Nebraska-Lincoln, USA.

Senaratne LS, Calkins C, Vasconcelos J, de Mello AS, Andersen MA, Furman SA, Pokharel S (2011) Shelf life of m. longissimus lumborum from beef fed antioxidants and wet distillers grains. Nebraska Beef Report MP94, 100–102.

Smith SB, Lunt DK, Chung KY, Choi CB, Tume RK, Zembayashi M (2006) Adiposity, fatty acid composition, and delta-9 desaturase activity during growth in beef cattle. Animal Science Journal 77, 478–486.
Adiposity, fatty acid composition, and delta-9 desaturase activity during growth in beef cattle.CrossRef | 1:CAS:528:DC%2BD28Xht1egs7nM&md5=2647061fa8713350882a8e1ebfb023abCAS |

Song R, Chen C, Wang L, Johnston L, Kerr B, Weber T, Shurson G (2013) High sulfur content in corn dried distillers grains with solubles protects against oxidized lipids by increasing sulfur-containing antioxidants in nursery pigs. Journal of Animal Science 91, 2715–2728.
High sulfur content in corn dried distillers grains with solubles protects against oxidized lipids by increasing sulfur-containing antioxidants in nursery pigs.CrossRef | 1:CAS:528:DC%2BC3sXpvFCrtr8%3D&md5=e72ec1a0755b2daa1d3fd6452f0900afCAS |

Stanley DW (1991) Biological membrane deterioration and associated quality losses in food tissues. Critical Reviews in Food Science and Nutrition 30, 487–553.
Biological membrane deterioration and associated quality losses in food tissues.CrossRef | 1:CAS:528:DyaK38Xitleqsbc%3D&md5=da172cc495d07fb29914cce84b3f9f34CAS |

Sun J, Xu L, Eu JP, Stamler JS, Meissner G (2001) Classes of thiols that influence the activity of the skeletal muscle calcium release channel. The Journal of Biological Chemistry 276, 15625–15630.
Classes of thiols that influence the activity of the skeletal muscle calcium release channel.CrossRef | 1:CAS:528:DC%2BD3MXjvFalu7w%3D&md5=b00f73e652fc1fba6bcf73483ad3f1d3CAS |

Taylor RG, Geesink G, Thompson V, Koohmaraie M, Goll D (1995) Is Z-disk degradation responsible for post-mortem tenderization? Journal of Animal Science 73, 1351–1367.
Is Z-disk degradation responsible for post-mortem tenderization?CrossRef | 1:CAS:528:DyaK2MXlsFSjtb0%3D&md5=add3dc4e6e48bdde56cd06b397592469CAS |

Thi-Dinh KK, Demarne Y, Nicolas C, Lhuillery C (1990) Effect of dietary fat on phospholipid class distribution and fatty acid composition in rat fat cell plasma membrane. Lipids 25, 278–283.
Effect of dietary fat on phospholipid class distribution and fatty acid composition in rat fat cell plasma membrane.CrossRef | 1:CAS:528:DyaK3cXktl2gsrw%3D&md5=4db7d921ff64bb78680b56d8729366f6CAS |

Wood JD, Enser M, Fisher AV, Nute GR, Sheard PR, Richardson RI, Hughes SI, Whittington FM (2008) Fat deposition, fatty acid composition and meat quality: a review. Meat Science 78, 343–358.
Fat deposition, fatty acid composition and meat quality: a review.CrossRef | 1:CAS:528:DC%2BD1cXhsVCls7s%3D&md5=5087ec4dbce501875a943f79ddaa74f8CAS |


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