Animal Production Science Animal Production Science Society
Food, fibre and pharmaceuticals from animals
RESEARCH ARTICLE

Effect of nano-selenium and nano-zinc particles during in vitro maturation on the developmental competence of bovine oocytes

B. R. Abdel-Halim A C and Nermeen A. Helmy B
+ Author Affiliations
- Author Affiliations

A Department of Theriogenology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

B Department of Physiology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt.

C Corresponding author. Email: drbakarwa@yahoo.com

Animal Production Science - https://doi.org/10.1071/AN17057
Submitted: 1 February 2017  Accepted: 9 June 2017   Published online: 4 August 2017

Abstract

The objectives of the current study were to evaluate the effects of supplemental nano-selenium (NSe) and nano-zinc oxide (NZn-O) particles during in vitro maturation (IVM) on DNA damage of cumulus cells, glutathione (GSH) concentration in bovine oocytes, subsequent embryo development and re-expansion rate of vitrified warmed blastocysts. The current study was conducted on bovine ovaries obtained from a local abattoir and transported to the laboratory in sterile phosphate buffer saline with antibiotics at 37°C, within 1 h after slaughter. Ovaries were pooled, regardless of stage of the oestrous cycle of the donor. Only cumulus-intact complexes with evenly granulated cytoplasm were selected for IVM. Experimental design included the following: Experiment 1 studied the effect of addition of 1.0 µg/mL NSe or NZn-O to IVM medium on DNA damage of cumulus cells; Experiment 2 evaluated the effects of NSe or NZn-O on intracellular glutathione in oocytes and cumulus cells; in Experiment 3, the development of oocytes matured in IVM medium supplemented with 1.0 µg/mL NSe or NZn-O was investigated; and in Experiment 4, the effects of adding 1.0 µg/mL NSe and NZn-O to in vitro fertilisation media on vitrified oocytes and embryos were investigated. The DNA damage in cumulus cells decreased with supplemental NSe and NZn-O at concentration of 1 µg/mL in the IVM medium (180.2 ± 21.4, 55.8 ± 4.3 and 56.6 ± 3.9 for the control and NSe and NZn-O groups respectively). Total GSH concentrations increased following supplementation with 1 µg/mL NSe and 1 µg/mL NZn-O, compared with the control group. Re-expansion rate of vitrified warmed blastocysts in experimental media containing NSe and NZn-O with ethylene glycol was higher than that of the control. In conclusion, providing NSe and NZn-O during oocyte maturation significantly increased both intracellular GSH concentration and DNA integrity of cumulus cells. Optimal embryo development was partially dependent on the presence of NSe and NZn-O during IVM. NSe and NZn-O during oocyte maturation act as a good cryoprotective agents of vitrified, warmed blastocysts.

Additional keywords: cattle IVP, in vitro embryo production, IVM, nanotechnology, vitrification.


References

Albanese A, Tang PS, Chan WC (2012) The effect of nanoparticle size, shape, and surface chemistry on biological systems. Annual Review of Biomedical Engineering 14, 1–16.
The effect of nanoparticle size, shape, and surface chemistry on biological systems.CrossRef | 1:CAS:528:DC%2BC38Xht1Oks7zF&md5=3d48033d61b3d33cbed3355e85345615CAS |

Checura CM, Seidel GE (2007) Effect of macromolecules in solutions for vitrification of mature bovine oocytes. Theriogenology 67, 919–930.
Effect of macromolecules in solutions for vitrification of mature bovine oocytes.CrossRef | 1:CAS:528:DC%2BD2sXitFOnsrc%3D&md5=f31ff8c5d4e5550aec1f34e91b61f6d0CAS |

Choi YH, Carnevale EM, Seidel GE, Squires EL (2001) Effect of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology 56, 661–670.
Effect of gonadotropins on bovine oocytes matured in TCM-199.CrossRef | 1:CAS:528:DC%2BD3MXntlahtrg%3D&md5=977992254d69944c7660365e5a097618CAS |

Chwa M, Atilano SR, Reddy V, Jordan N, Kim DW, Kenney MC (2006) Increased stress-induced generation of reactive oxygen species and apoptosis in human keratoconus fibroblasts. Investigative Ophthalmology & Visual Science 47, 1902–1910.
Increased stress-induced generation of reactive oxygen species and apoptosis in human keratoconus fibroblasts.CrossRef |

Collins AR (2004) The comet assay for DNA damage and repair: principles, applications, and limitations. Molecular Biotechnology 26, 249–261.
The comet assay for DNA damage and repair: principles, applications, and limitations.CrossRef | 1:CAS:528:DC%2BD2cXivF2gu7c%3D&md5=bf2d8563c49aa86eeaa07bb6b0438a33CAS |

Córdova B, Morató R, Izquierdo D, Paramio T, Mogas T (2010) Effect of the addition of insulin–transferrin–selenium and/or L-ascorbic acid to the in vitro maturation of prepubertal bovine oocytes on cytoplasmic maturation and embryo development. Theriogenology 74, 1341–1348.
Effect of the addition of insulin–transferrin–selenium and/or L-ascorbic acid to the in vitro maturation of prepubertal bovine oocytes on cytoplasmic maturation and embryo development.CrossRef |

Cuello C, Berthelot F, Delaleu B, Venturi E, Pastor LM, Vazquez JM, Martinez EA (2007) The effectiveness of the stereomicroscope evaluation of embryo quality in vitrified-warmed porcine blastocysts: an ultrastructure and cell death study. Theriogenology 67, 970–982.
The effectiveness of the stereomicroscope evaluation of embryo quality in vitrified-warmed porcine blastocysts: an ultrastructure and cell death study.CrossRef | 1:STN:280:DC%2BD2s7hsFenuw%3D%3D&md5=aad956f12b2676412b23df8c2a1fcc4aCAS |

Dalvit G, Llanes S, Descalzo A, Insani M, Beconi M, Cetica P (2005) Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation. Reproduction in Domestic Animals 40, 93–97.
Effect of alpha-tocopherol and ascorbic acid on bovine oocyte in vitro maturation.CrossRef | 1:CAS:528:DC%2BD2MXktFalsLc%3D&md5=23e02b983f7ae887e5cc9028b6c0268dCAS |

Ebert R, Ulmer M, Zeck S, Meissner-Weig J, Schneider D, Stopper H, Schupp N, Kassem M, Jakob F (2006) Selenium supplementation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro. Stem Cells 24, 1226–1235.
Selenium supplementation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro.CrossRef | 1:CAS:528:DC%2BD28XhtFGhu7bL&md5=2230017bd5bc7c64dc6690a54c5c325aCAS |

Furnus CC, de Matos DG, Moses DF (1998) Cumulus expansion during in vitro maturation of bovine oocytes: relationship with Intracellular glutathione level and its role on subsequent embryo development. Molecular Reproduction and Development 51, 76–83.
Cumulus expansion during in vitro maturation of bovine oocytes: relationship with Intracellular glutathione level and its role on subsequent embryo development.CrossRef | 1:CAS:528:DyaK1cXltVGrsLk%3D&md5=f381fe2190027f804206a09b9666c62eCAS |

Gardner DK, Lane M, Spitzer A, Batt PA (1994) Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development. Biology of Reproduction 50, 390–400.
Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulate development.CrossRef | 1:STN:280:DyaK2c7ptFWisA%3D%3D&md5=687c4f12d1e4551ff06c4e3c04af9b0bCAS |

Ghorbani HR, Mehr FP, Pazoki H, Rahmani BM (2015) Synthesis of ZnO nanoparticles by precipitation method. Oriental Journal of Chemistry 31, 1219–1221.
Synthesis of ZnO nanoparticles by precipitation method.CrossRef | 1:CAS:528:DC%2BC2MXhtlOltbfN&md5=929f5cbbefa513ea23c54c7acebd06a5CAS |

Hensleigh HC, Hunter AG (1985) IVM of bovine cumulus enclosed primary oocytes and their subsequent IVF and cleavage. Journal of Dairy Science 68, 1456–1462.
IVM of bovine cumulus enclosed primary oocytes and their subsequent IVF and cleavage.CrossRef | 1:STN:280:DyaL2M3mt1Wqtg%3D%3D&md5=6df2d2b9505a9166831af54a1a84ec23CAS |

Hostetler CE, Kincaid RL, Mirando MA (2003) The role of essential trace elements in embryonic and fetal development in livestock. Veterinary Journal 166, 125–139.
The role of essential trace elements in embryonic and fetal development in livestock.CrossRef | 1:CAS:528:DC%2BD3sXlvFOrs7g%3D&md5=72b04f09a7c3ee8c126c58dc3734b5a3CAS |

Kambe T, Weaver BP, Andrews GK (2008) The genetics of essential metal homeostasis during development. Genesis 46, 214–228.
The genetics of essential metal homeostasis during development.CrossRef | 1:CAS:528:DC%2BD1cXmvVSgtb8%3D&md5=57d267e896fc49fe68d7b2739864574bCAS |

Lindner GM, Wright RW (1983) Bovine embryo morphology and evaluation. Theriogenology 20, 407–416.
Bovine embryo morphology and evaluation.CrossRef | 1:STN:280:DC%2BD283pvVKgsg%3D%3D&md5=19b02b10ff1d2fc1b88bdbf0e727a924CAS |

Nedambale TL, Du F, Xu J, Chaubal SA, Dinneyes A, Tian XC (2006) Prolonging bovine serum: oocyte incubation in modified medium 199 improves embryo development rate and the viability of vitrified blastocyst. Theriogenology 66, 1951–1960.
Prolonging bovine serum: oocyte incubation in modified medium 199 improves embryo development rate and the viability of vitrified blastocyst.CrossRef | 1:STN:280:DC%2BD28nlsF2isA%3D%3D&md5=cf704600eedcba1261924d1033b35a8bCAS |

Ong C, Lee QY, Cai Y, Liu X, Ding J, Yung L-Y, Bay B-H, Baeg GH (2016) Silver nanoparticles disrupt germline stem cell maintenance in the Drosophila testis. Scientific Reports 6, 20632
Silver nanoparticles disrupt germline stem cell maintenance in the Drosophila testis.CrossRef | 1:CAS:528:DC%2BC28Xitlygtr4%3D&md5=c0c587eae3144f2567d1b3eff953f43bCAS |

Parrish JJ, Susko-Parrish JL, Liebfried-Rutledge L, Crister ES, Eyestone WH, Frist NL (1986) Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25, 591–600.
Bovine in vitro fertilization with frozen-thawed semen.CrossRef | 1:STN:280:DC%2BD283pvV2itQ%3D%3D&md5=185d5c119b5b267340e15c15a2802123CAS |

Peng D, Zhang J, Liu Q, Taylor EW (2007) Size effect of elemental selenium nanoparticles (nano-Se) at supra nutritional levels on selenium accumulation and glutathione S-transferase activity. Journal of Inorganic Biochemistry 101, 1457–1463.
Size effect of elemental selenium nanoparticles (nano-Se) at supra nutritional levels on selenium accumulation and glutathione S-transferase activity.CrossRef | 1:CAS:528:DC%2BD2sXhtVWht7nO&md5=8873e07d18e47f8be8cb1c7c3372d0b8CAS |

Picco SJ, Anchordoquy JM, de Matos DG, Anchordoquy JP, Seoane A, Mattioli GA, Errecalde AL, Furnus CC (2010) Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes. Theriogenology 74, 1141–1148.
Effect of increasing zinc sulphate concentration during in vitro maturation of bovine oocytes.CrossRef | 1:CAS:528:DC%2BC3cXhtFOrs7jL&md5=5ed53386bc73ecab39c8130daea6cb42CAS |

Sabat D, Patnaik A, Ekka B, Dash P, Mishra M (2016) Investigation of titania nanoparticles on behavior and mechanosensory organ of Drosophila melanogaster. Physiology & Behavior 167, 76–85.
Investigation of titania nanoparticles on behavior and mechanosensory organ of Drosophila melanogaster.CrossRef | 1:CAS:528:DC%2BC28XhsFSlt77E&md5=a83ab1735a1cecd48e4b1b3d2cd52208CAS |

Sabella S, Carney RP, Brunetti V, Malvindi MA, Al-Juffali N, Vecchio G, Janes SM, Bakr OM, Cingolani R, Stellacci F (2014) A general mechanism for intracellular toxicity of metal-containing nanoparticles. Nanoscale 6, 7052–7061.
A general mechanism for intracellular toxicity of metal-containing nanoparticles.CrossRef | 1:CAS:528:DC%2BC2cXptVeis7k%3D&md5=ee6491f7accc3b7d544b87f5ec48b22fCAS |

Sarkar B, Bhattacharjee S, Daware A, Tribedi P, Krishnani KK, Minhas PS (2015) Selenium nanoparticles for stress-resilient fish and livestock. Nanoscale Research Letters 10, 371–380.
Selenium nanoparticles for stress-resilient fish and livestock.CrossRef |

Schellander K, Fayer-Hosken RA, Keefer CL, Brown LM, Malter H, McBride CE, Brackett BG (1989) In vitro fertilization of bovine follicular oocytes recovered by laparoscopy. Theriogenology 31, 927–934.
In vitro fertilization of bovine follicular oocytes recovered by laparoscopy.CrossRef | 1:STN:280:DC%2BD283pvFeksA%3D%3D&md5=c279277e4d95308d8e192a4b789d355bCAS |

Sies H (1999) Glutathione and its role in cellular functions. Free Radical Biology & Medicine 27, 916–921.
Glutathione and its role in cellular functions.CrossRef | 1:CAS:528:DyaK1MXns1ymsrw%3D&md5=02982ccace9439bf4a921696524cbd0eCAS |

Sirard MA (2001) Resumption of meiosis: mechanism involved in meiotic progression and its relation with developmental competence. Theriogenology 55, 1241–1254.
Resumption of meiosis: mechanism involved in meiotic progression and its relation with developmental competence.CrossRef | 1:CAS:528:DC%2BD3MXjsFSisr0%3D&md5=12701090c69cfd2fb5d44cda3d642b6dCAS |

Somfai T, Bodó S, Nagy S, Papp AB, Iváncsics J, Baranyai B, Gócza E, Kovács A (2002) Effect of swim-up and Percoll treatment on viability and acrosome integrity of frozen-thawed bull spermatozoa. Reproduction in Domestic Animals 37, 285–290.
Effect of swim-up and Percoll treatment on viability and acrosome integrity of frozen-thawed bull spermatozoa.CrossRef | 1:STN:280:DC%2BD38njtlWgug%3D%3D&md5=e895922982a4c3221919d468e32d2490CAS |

Stephenson JL, Brackett BG (1999) Influences of Zinc on fertilization and development of bovine oocytes in vitro. Zygote (Cambridge, England) 7, 195–201.
Influences of Zinc on fertilization and development of bovine oocytes in vitro.CrossRef | 1:CAS:528:DyaK1MXmvFaltro%3D&md5=556a1fafde42b0e5d0a60470c75e9233CAS |

Tatham B, Feehan T, Pashen R (2003) Buffalo and cattle hybrid embryo development is decreased by caffeine treatment during in vitro fertilization. Theriogenology 59, 709–717.
Buffalo and cattle hybrid embryo development is decreased by caffeine treatment during in vitro fertilization.CrossRef | 1:CAS:528:DC%2BD3sXnvF2i&md5=62c05c556a0a27f5f89ff6fb0bc47c54CAS |

Thompson JG (2000) In vitro culture and embryo metabolism of cattle and sheep embryos: a decade of achievement. Animal Reproduction Science 60–61, 263–275.
In vitro culture and embryo metabolism of cattle and sheep embryos: a decade of achievement.CrossRef |

Totey SM, Daliri M, Appo Rao KBC, Pawshe CH, Taneja M (1996) Differential cleavage and developmental rates and their correlation with cell number and sex ratios in buffalo embryo generated in vitro. Theriogenology 45, 521–533.

Vanderzwalmen P, Connan D, Grobet L, Wirleitner B, Remy B, Vanderzwalmen S, Zech N, Ectors FJ (2013) Lower intracellular concentration of cryoprotectants after vitrification than after slow freezing despite exposure to higher concentration of cryoprotectant solutions. Human Reproduction 28, 2101–2110.
Lower intracellular concentration of cryoprotectants after vitrification than after slow freezing despite exposure to higher concentration of cryoprotectant solutions.CrossRef | 1:CAS:528:DC%2BC3sXhtFOgtrrK&md5=937d3740cdf29b693e4dd34f19bcf112CAS |

Wu MC, Lee H-M (1996) ‘Vitrification of porcine oocytes.’ Contribution no. 683. (Taiwan Livestock Research Institute)

Wu D, Cheung QC, Wen L, Li J (2006) A growth-maturation system that enhances the meiotic and developmental competence of porcine oocytes isolated from small follicles. Biology of Reproduction 75, 547–554.

Yang Y, Oztekin A, Neti S, Mohapatra S (2012) Particle agglomeration and properties of nanofluids. Journal of Nanoparticle Research 14, 852
Particle agglomeration and properties of nanofluids.CrossRef |

Younis AI, Brackett BG, Fayer-Hosken RA (1989) Influence of serum and hormones on bovine oocytes maturation and fertilization in vitro. Gamete Research 23, 189–201.
Influence of serum and hormones on bovine oocytes maturation and fertilization in vitro.CrossRef | 1:CAS:528:DyaL1MXksFSmt7w%3D&md5=7bf0de3eb102fd98870ebeb187ffaee3CAS |

Zhang S, Gao XY, Zhang LD, Bao YP (2001) Biological effects of a nano red elemental selenium. BioFactors 15, 27–38.
Biological effects of a nano red elemental selenium.CrossRef |

Zhang J, Robinson D, Salmon P (2006) A novel function for selenium in biological system: selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production. Biotechnology and Bioengineering 95, 1188–1197.
A novel function for selenium in biological system: selenite as a highly effective iron carrier for Chinese hamster ovary cell growth and monoclonal antibody production.CrossRef | 1:CAS:528:DC%2BD28Xht1Grt7fL&md5=6969f5a8e85d0cf306730267ec4cc124CAS |



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