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Histomorphometric analysis of the effect of in ovo injected methionine on small intestine in broiler chicken embryo

Mohammad Naser Nazem , Sayed Mohsen Sajjadian , Reza Kheirandish , Hamideh Mohammadrezaei

Abstract

This study evaluated the histomorphometric effect on the small intestine of the chicken embryo after in-ovo methionine injection. On day four of incubation, fifty fertile eggs were allocated into one of 5 groups: Control (no treatment) and four treatment groups which received either 20, 30, 40 or 50 mg methionine via their yolk sac. All eggs were incubated until day 19 at which point the embryos were terminated and 1cm samples of the duodenum, jejunum and ileum were taken for histology. Sections were stained by haematoxylin & eosin (H&E), Alcian blue and periodic acid Schiff (PAS) methods separately. Morphometric analysis was carried out to assess goblet cell number, enterocyte height, muscle layer thickness as well as villus height, width, area and shape. The ratio of embryo body weight to egg weight in methionine treatment groups was more than in controls and this difference was greatest in the 40 mg methionine group. The results showed that villous height, width and area increased in treatment groups, as did enterocyte height, goblet cell number and muscle layer thickness. The ratio and sequence of the villi was also changed in some treatments. Our results indicated that injecting methionine into the yolk sac can improve intestinal histomorphometrical parameters and that 40 mg methionine showed the greatest changes.

AN17269  Accepted 16 September 2017

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