Diphenyl diselenide subcutaneous supplementation of dairy sheep: effects on oxidant and antioxidant status, inflammatory response and milk composition
Angelisa H. Biazus A , Chrystian J. Cazarotto A , Gustavo Machado B , Nathieli B. Bottari C , Mariana S. Alves C , Vera M. Morsch C , Maria R. C. Schetinger C , Marta L. R. Leal D , Natieli F. Fernandes E , Rafael N. Moresco E , Matheus D. Baldissera F and Aleksandro S. Da Silva B C G
+ Author Affiliations
- Author Affiliations
A Graduate Program in Animal Science and Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Santa Catarina (SC), Brazil.
B Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, USA.
C Graduate Program in Toxicological Biochemical and Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
D Department of Large Animal Medicine (UFSM), Santa Maria, RS, Brazil.
E Department of Clinical and Toxicological Analysis (UFSM), Santa Maria, RS, Brazil.
F Department of Microbiology and Parasitology (UFSM), Santa Maria, RS, Brazil.
G Corresponding author. Email: aleksandro_ss@yahoo.com.br
Animal Production Science 59(3) 461-470 https://doi.org/10.1071/AN17374
Submitted: 5 June 2017 Accepted: 18 January 2018 Published: 16 April 2018
Abstract
Diphenyl diselenide ((PhSe)2) is a organoselenium compound with potent antioxidant properties. Therefore, the aim of the present study was to evaluate whether subcutaneous supplementation of (PhSe)2 in dairy sheep has positive effects on milk composition, as well as on the prevention of oxidative stress and exacerbated inflammatory response. For this, 16 primiparous recently calved sheep were divided into the following two groups, with eight animals in each: Group A, the control group; and Group B, the group subcutaneously supplemented with five doses of (PhSe)2 of 3.0 µmol/kg each every 7 days. Blood samples from supplemented animals showed increased concentration of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase), and reduced reactive oxygen species and lipid peroxidation, which prevented oxidative damage in the lactation period, as well as increased seric interleukin-10, an anti-inflammatory cytokine. In the sera, supplemented animals showed increased total antioxidant capacity and ferric-reducing ability of plasma compared with the control group. As a consequence, supplemented animals showed increased antioxidant variables, as well as reduced protein oxidation in milk samples. Moreover, milk from supplemented sheep showed a higher fat content, and lower total protein and lactose contents in some periods in the study, than did not-supplemented ewes. Seric concentrations of interleukin-1 were lower on Days 30 and 45 in supplemented animals, as well as the concentrations of tumour necrosis factor α in all periods, than were those in the control group, whereas the interleukin-10 concentrations were higher. Thus, dairy sheep supplementation of (PhSe)2 activated antioxidant and anti-inflammatory responses, and increased milk fat content. Moreover, this protocol increased the antioxidant and, consequently, reduced the oxidant concentration in milk, which is desirable for product quality.
Additional keywords: biochemistry, selenium, sheep milk.
References
Aebi H (1984) Catalase in vitro. Methods in Enzymology 105, 121–126.| Catalase in vitro.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXltVKis7s%3D&md5=a2df45d7f6aaf389e3cf2d49e18bd4ecCAS |
Arababadi MK, Mosavi R, Khorramdelazad H, Yaghini N, Zarandi ER, Araste M, Pourali R, Nekhei Z, Kennedy D (2010) Cytokine patterns after therapy with Avonex®, Rebif®, Betaferon® and CinnoVex® in relapsing-remitting multiple sclerosis in Iranian patients. Biomarkers in Medicine 4, 755–759.
| Cytokine patterns after therapy with Avonex®, Rebif®, Betaferon® and CinnoVex® in relapsing-remitting multiple sclerosis in Iranian patients.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht1yqtrnP&md5=8a6f13ecf55c265e6b730ce56e370dccCAS |
Armstrong D, Browne R (1998) Synthesis of lipid and cholesterol hydroperoxide standards. Free radical and antioxidant protocols. Free radical and antioxidant protocols. 108, 139–145.
Barbosa CF, Tonin AA, Da Silva AS, Azevedo MI, Monteiro DU, Waczuk EP, Duarte T, Hermes C, Camillo G, Vogel FF, Faccio L, Tonin PT, Wolkmer P, Leal MR, Duarte MMF, Moresco RN, Lopes STA, De La Rue ML (2014) Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation. Parasitology 141, 1761–1768.
| Diphenyl diselenide and sodium selenite associated with chemotherapy in experimental toxoplasmosis: influence on oxidant/antioxidant biomarkers and cytokine modulation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhs1KmsrbL&md5=eb073d7962f2e3ce0ecd964ef99272ebCAS |
Benzie IFF, Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299, 15–27.
| Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXpsFag&md5=8a6c3eabe011f0a8f1e529fa919f6519CAS |
Bianchi MLP, Antunes LMG (1999) Radicais livres e os principais antioxidantes da dieta. Revista de Nutrição 12, 123–130.
| Radicais livres e os principais antioxidantes da dieta.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1Ghsbw%3D&md5=a3b290cba10ef1e5f0289b792bbe1619CAS |
Butler WR (2000) Nutritional interactions with reproductive performance in dairy cattle. Animal Reproduction Science 60–61, 449–457.
| Nutritional interactions with reproductive performance in dairy cattle.Crossref | GoogleScholarGoogle Scholar |
Castillo C, Hernandez J, Valverde I, Pereira V, Sotillo J, López AM, Benedito JL (2006) Plasma malondialdehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows. Research in Veterinary Science 80, 133–139.
| Plasma malondialdehyde (MDA) and total antioxidant status (TAS) during lactation in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXht12qtb7P&md5=620f85b1ab1acdc15a057b90221eb363CAS |
Clausen MR, Connolly C, Skibsted LH, Stagsted J (2010) Oxidative stability of bovine milk determined by individual variability in herd irrespective of selenium status. International Dairy Journal 20, 507–513.
| Oxidative stability of bovine milk determined by individual variability in herd irrespective of selenium status.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmsVertLc%3D&md5=a5ab5d7b27dde8dcc033a9b1e03ad806CAS |
Colpo E, De Bem AF, Pieniz S, Schettert SD, dos Santos RM, Farias IL, Bertoncello I, Moreira CM, Barbosa NV, Moretto MB, Rocha JB (2008) A single high dose of ascorbic acid and iron is not correlated with oxidative stress in healthy volunteers. Annals of Nutrition and Metabolism 53, 79–85.
Cominetti C, Cozzolino SMF (2009) Funções Plenamente Reconhecidas de Nutrientes – Selênio. (ILSI: São Paulo, Brasil)
de Bem AF, Farina M, Portella L, Nogueira CW, Dinis TC, Laranjinha JA, Almeida LM, Texeira-Rocha JB (2008) Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro. Atherosclerosis 201, 92–100.
| Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1KltrzP&md5=19577dac475bb59683fefc3432e10eaaCAS |
de Bem AF, Portella L, Colpo E, Duarte MM, Frediane A, Taube PS, Nogueira CW, Farina M, Da Silva EL, Teixeira-Rocha JB (2009) Diphenyl diselenide decreases serum levels of total cholesterol and tissue oxidative stress in cholesterol-fed rabbits. Basic & Clinical Pharmacology & Toxicology 105, 17–23.
| Diphenyl diselenide decreases serum levels of total cholesterol and tissue oxidative stress in cholesterol-fed rabbits.Crossref | GoogleScholarGoogle Scholar |
Drackley JK (1999) Biology of dairy cows during the transition period: the final frontier? Journal of Dairy Science 82, 2259–2273.
| Biology of dairy cows during the transition period: the final frontier?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnsVGntLY%3D&md5=cf2742976defddd45eaafd7e75910dc6CAS |
Erel O (2004) A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry 37, 277–285.
| A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhslWmtLc%3D&md5=613efbe4c72777d9648ec39db3983a5aCAS |
Faixová Z, Piešová E, Maková Z, Čobanová K, Faix S (2016) Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep. Acta Veterinaria Brno 85, 185–194.
| Effect of dietary supplementation with selenium-enriched yeast or sodium selenite on ruminal enzyme activities and blood chemistry in sheep.Crossref | GoogleScholarGoogle Scholar |
Ferreira ALA, Matsubara LS (1997) Radicais livres: conceitos, doenças relacionadas, sistema de defesa e estresse oxidativo. Revista da Associação Médica do Brasil 43, 61–68.
Fiorentino DF, Bond MW, Mosmann TR (1989) Two types of mouse helper T cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Thl clones. The Journal of Experimental Medicine 170, 2081–2095.
| Two types of mouse helper T cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Thl clones.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXhtFKqtL0%3D&md5=ca052ec0d6c515b1ea4d639e140fa2f6CAS |
Flores EMM, Saidelles APF, Barin JS, Mortari SR, Martins AF (2001) Hair sample decomposition using polypropylene vials for determination of arsenic by hydride generation atomic absorption spectrometry. Journal of Analytical Atomic Spectrometry 16, 1419–1423.
| Hair sample decomposition using polypropylene vials for determination of arsenic by hydride generation atomic absorption spectrometry.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXovFKiurY%3D&md5=53e959fffd9b47af3a278bba469ccf9bCAS |
Fonseca SF, Lima DB, Alves D, Jacob RG, Perin G, Lenardão EJ, Savegnano L (2015) Synthesis, characterization and antioxidante activity of organoselenium and organotellurium compound derivatives of chrysin. New Journal of Chemistry 39, 3043–3050.
| Synthesis, characterization and antioxidante activity of organoselenium and organotellurium compound derivatives of chrysin.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXitlaltbg%3D&md5=10206399d9aa8219a912c33bdbf9c672CAS |
Ghisleni G, Porciuncula LO, Cimarosti H, Rocha JBT, Salbego CG, Souza DO (2003) Diphenyl diselenide protect rat hippocampal slices submitted to oxygen-glucose deprivation and diminishes inducible nitric oxide synthase immunocontent. Brain Research 986, 196–199.
| Diphenyl diselenide protect rat hippocampal slices submitted to oxygen-glucose deprivation and diminishes inducible nitric oxide synthase immunocontent.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXntVymt7w%3D&md5=69f731d9082754f18bfec19bbdad2110CAS |
Givens DI, Allison R, Cottrill B, Blake JS (2004) Enhancing the selenium content of bovine milk through alteration of the form and concentration of selenium in the diet of the dairy cow. Journal of the Science of Food and Agriculture 84, 811–817.
| Enhancing the selenium content of bovine milk through alteration of the form and concentration of selenium in the diet of the dairy cow.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXkvVCiu7w%3D&md5=8e1a7b2d4d60f1103837637f6994f0b4CAS |
Grilli E, Gallo A, Fustini M, Fantinati P, Piva A (2013) Microencapsulated sodium selenite supplementation in dairy cows: effects on selenium status. Animal 7, 1944–1949.
| Microencapsulated sodium selenite supplementation in dairy cows: effects on selenium status.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhsl2ht7fK&md5=f91553a5f1a842d31c3691fb11b69dc6CAS |
Gröhn YT, Erb HN, Mcculloch CE, Saloniemi HS (1989) Epidemiology of metabolic disorders of dairy cattle: Association among host characteristics, disease, and production. Journal of Dairy Science 72, 1876–1885.
| Epidemiology of metabolic disorders of dairy cattle: Association among host characteristics, disease, and production.Crossref | GoogleScholarGoogle Scholar |
Guzzo N, Bailoni L, Mantovani R, Da Dalt L, Gabai G (2015) Oxidized protein biomarkers in the blood and milk of cows supplemented with flaxseed during the dry period. In ‘2nd DairyCare conference’, 3–4 March 2015, Cordoba, Spain. DairyCare COST Action FA1308, 60. (Ed. CH Knight)
Habig WH, Pabst MJ, Jakob WB (1974) Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. The Journal of Biological Chemistry 249, 7130–7139.
Halliwell B, Whiteman M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? British Journal of Pharmacology 142, 231–255.
| Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXkvVyks7w%3D&md5=5ed34d3703aec727cb81ac9fd5895e51CAS |
Harrison JP, Hancock DD, Conrad HR (1984) Vitamin E and selenium for reproduction of the dairy cow. Journal of Dairy Science 67, 123–132.
| Vitamin E and selenium for reproduction of the dairy cow.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXhtF2ksL8%3D&md5=e760f4f457ee2d1027ce2a09e1e43f9eCAS |
Hoekstra WG (1975) Biochemical function of selenium and it’s relation to vitamin E. Federation Proceedings Journal 34, 2083–2090.
Hoffmann PR (2007) Mechanisms by which selenium influences immune responses. Archivum Immunologiae et Therapiae Experimentalis 55, 289–297.
| Mechanisms by which selenium influences immune responses.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtlGqsL%2FL&md5=008cff64b93e67e459aa89de48451896CAS |
Islam R, Kumar H, Nandi S, Rai RB (2013) Determination of anti-inflammatory cytokine in periparturient cows for prediction of postpartum reproductive diseases. Theriog. 79, 974–979.
| Determination of anti-inflammatory cytokine in periparturient cows for prediction of postpartum reproductive diseases.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXivF2is7c%3D&md5=816d67812b9c3b069be4448b05b13aceCAS |
Jentzsch AM, Bachmann H, Furst P, Biesalski H (1996) Improved analysis of malondialdehyde in human body fluids. Free Radical Biology & Medicine 20, 251–256.
| Improved analysis of malondialdehyde in human body fluids.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XjsVartQ%3D%3D&md5=2d969d330719bce1c49b11c9541db1e3CAS |
Hayes JD, Flanagan JU, Jowsey IR (2005) Glutathione transferases. Annual Review of Pharmacology and Toxicology 45, 51–88.
| Glutathione transferases.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXisVWjtrk%3D&md5=6d8f6737333bda240e65519b09fe8e25CAS |
Lomba F (1996) Influence of dietary cation-anion and oxidative-antioxidants balances on diseases occurring around parturition in the dairy cows. Annales de Medecine Veterinaire 140, 109–122.
Luchese C, Prigol M, Duarte MMMF, Nogueira CW (2012) Diphenyl diselenide reduces inflammation in the mouse model of pleurisy induced by carrageenan: reduction of pro-inflammatory markers and reactive species levels. Inflammation Research 61, 1117–1124.
| Diphenyl diselenide reduces inflammation in the mouse model of pleurisy induced by carrageenan: reduction of pro-inflammatory markers and reactive species levels.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhtleiu7nM&md5=bdb6c2149282bc114c936f91f2e35e5fCAS |
McCords MJ, Fridovich I (1969) Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). The Journal of Biological Chemistry 244, 6049–6065.
McDowell LR (1992) ‘Minerals in animal and human nutrition.’ (Academic Press)
Meotti FC, Stangherlin EC, Zeni G, Nogueira CW, Rocha JBT (2004) Protective role of aryl and alkyl diselenides on lipid peroxidation. Environmental Research 94, 276–282.
| Protective role of aryl and alkyl diselenides on lipid peroxidation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhvV2htL4%3D&md5=348826b96003a6560f76267069066b8eCAS |
Nogueira CW, Meotti FC, Pilissão C, Zeni G, Rocha JBT (2003) Investigations into the potential neurotoxicity induced by diselenides in mice and rats. Toxicology 183, 29–37.
| Investigations into the potential neurotoxicity induced by diselenides in mice and rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XpslSjsbw%3D&md5=8443fce31add0e63a4ba3186e779c5d1CAS |
Nogueira CW, Quinhones EB, Jung EA, Zeni G, Rocha JBT (2003b) Anti-inflammatory and antinociceptive activity of diphenyl diselenide. Inflammation Research 52, 56–63.
| Anti-inflammatory and antinociceptive activity of diphenyl diselenide.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXitlOksbk%3D&md5=28f8e449e19f2784720818ece057b7daCAS |
Nogueira CW, Zeni G, Rocha JBT (2004) Organoselenium and organotellurium compounds: toxicology and pharmacology. Chemical Reviews 104, 6255–6286.
| Organoselenium and organotellurium compounds: toxicology and pharmacology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXpt1SktLw%3D&md5=61329e398fb00db4fecdc0e5415eae57CAS |
Paglia D, Valentine W (1967) Studies on the quantitative and qualitative characterization of erythtrocyte glutathione peroxidase. The Journal of Laboratory and Clinical Medicine 70, 158–169.
Petronilho F, Florentino D, Silvestre F, Danielski LG, Nascimento DZ, Vieira A, Kanis LA, Fortunato JJ, Badawy M, Barichello T, Quevedo J (2015) Ebselen attenuates lung injury in experimental model of carrageenan-induced pleurisy in rats. Inflammation 38, 1394–1400.
| Ebselen attenuates lung injury in experimental model of carrageenan-induced pleurisy in rats.Crossref | GoogleScholarGoogle Scholar |
Pivoto F, Leal MLR, Rocha JB, Aires A, Rocha J, Stefanello S, Carpes JLS (2017) Distribuição do selênio em ovinos tratados com disseleneto de difenila. Arquivo Brasileiro de Medicina Veterinária e Zootecnia in press.
Prigol M, Bruning CA, Zeni G, Nogueira CW (2009) Protective effect of disubstituted dayryl diselenides on cerebral oxidative damage caused by sodium nitroprusside. Biochemical Engineering Journal 45, 94–99.
| Protective effect of disubstituted dayryl diselenides on cerebral oxidative damage caused by sodium nitroprusside.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXlsVektLg%3D&md5=a5783e95a01a1cefebc97212a570d016CAS |
Radavelli WM, Campigotto G, Machado G, Bottari NB, Bochi G, Moresco RN, Morsch VM, Schetinger MRC, Bianchi A, Baldissera MD, Ferreira R, Da Silva AS (2016) Effect of lactation induction on milk production and composition, oxidative and antioxidant status, and biochemical variables. Comparative Clinical Pathology 25, 639–648.
| Effect of lactation induction on milk production and composition, oxidative and antioxidant status, and biochemical variables.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28Xjslyjurg%3D&md5=e0f29eadcc92bde21331b509d48508afCAS |
Ran L, Wu X, Shen X, Zhang K, Ren F, Huang K (2010) Effects of selenium form on blood and milk selenium concentrations, milk component and milk fatty acid composition in dairy cows. Journal of the Science of Food and Agriculture 90, 2214–2219.
| Effects of selenium form on blood and milk selenium concentrations, milk component and milk fatty acid composition in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtFGqtr%2FO&md5=2ea740a813632e3c89308425aa687038CAS |
Rizzo A, Pantaleo M, Mutinati M, Minoia G, Trisolini C, Ceci E, Sciorsci RL (2013) Blood and milk oxidative status after administration of different antioxidants during early postpartum in dairy cows. Research in Veterinary Science 95, 1171–1174.
| Blood and milk oxidative status after administration of different antioxidants during early postpartum in dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXht12itL3K&md5=2124b95f727b2ec3a15b9e38eb972cdeCAS |
Rocha JFX, Aires AR, Mendes FC, Pivoto FL, Amaral C, Torbitz VD, Moresco R, Nunes MAG, Flores EMM, Sousa RS, Rocha RX, Leal MLR (2017) Metaphylactic effect of injectable zinc on metabolic and oxidative parameters of sheep in the immediate postpartum. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, 1062–1065.
| Metaphylactic effect of injectable zinc on metabolic and oxidative parameters of sheep in the immediate postpartum.Crossref | GoogleScholarGoogle Scholar |
Roesler R, Malta LG, Carrasco LC, Holanda RB, Sousa CAS, Pastore GM (2007) Antioxidant activity of native fruits. Cien. Tecnol. Alim. 27, 53–60.
| Antioxidant activity of native fruits.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXltlOmsL0%3D&md5=c80b74bcbe749695bce3f072ce7d3f7fCAS |
Savegnago L, Pinto LG, Jesse CR, Alves D, Rocha JBT, Nogueira CW, Zeni G (2007) Antinociceptive properties of diphenyl diselenide: evidences for the mechanism of action. European Journal of Pharmacology 555, 129–138.
| Antinociceptive properties of diphenyl diselenide: evidences for the mechanism of action.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXls1Cgtw%3D%3D&md5=893f074f696c975aeb63151638b3be18CAS |
Silanikove N, Shapiro F, Shamay A, Leitner G (2005) Role of xanthine oxidase, lactoperoxidase, and NO in the innate immune system of mammary secretion during active involution in dairy cows: manipulation with casein hydrolyzates. Free Radical Biology & Medicine 38, 1139–1151.
| Role of xanthine oxidase, lactoperoxidase, and NO in the innate immune system of mammary secretion during active involution in dairy cows: manipulation with casein hydrolyzates.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXivFSjur8%3D&md5=d71c79600ab1b2c7ace72d33efc6b6e5CAS |
Silvestre F, Danielski LG, Michels M, Florentino D, Vieira A, Souza L, Cardoso LC, Schraiber R, Rezin GT, Vuolo F, da Rocha JB, Barichello T, Quevedo J, Dal-Pizzol F, Petronilho F (2014) Effects of organoselenium compounds on early and late brain biochemical alterations in sepsis-survivor rats. Neurotoxicity Research 26, 382–391.
| Effects of organoselenium compounds on early and late brain biochemical alterations in sepsis-survivor rats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXotFSqtro%3D&md5=fff661eb0eef7fc11b202f8206cd9608CAS |
Smet K, Raes K, De Block J, Herman L, Dewettinck K, Coudijzer K (2008) A change in antioxidative capacity as a measure of onset to oxidation in pasteurized milk. International Dairy Journal 18, 520–530.
| A change in antioxidative capacity as a measure of onset to oxidation in pasteurized milk.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXjtl2gt7k%3D&md5=0a6b8f6394b06b3bbff023d21ba5372dCAS |
Smith KL, Harrison JH, Hancock DD, Todhunter DA, Conrad HR (1984) Effect of vitamin E and selenium supplementation on incidence of clinical mastitis and duration of clinical symptoms. Journal of Dairy Science 67, 1293–1300.
| Effect of vitamin E and selenium supplementation on incidence of clinical mastitis and duration of clinical symptoms.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXkvV2gtr8%3D&md5=615f1f4f52d678b0d302c1e5da56f21fCAS |
Sordillo LM (2013) Selenium-dependent regulation of oxidative stress and immunity in periparturient dairy cattle. Veterinary Medicine International 2013, 1–8.
| Selenium-dependent regulation of oxidative stress and immunity in periparturient dairy cattle.Crossref | GoogleScholarGoogle Scholar |
Soriano VS, Jonathan S, Junior HPR, Torbitz VD, Moresco RN, Stefani LM, Da Silva AS (2015) Postpartum nitric oxide, oxidants and antioxidants levels in ewes and their lambs. Small Ruminant Research 123, 13–16.
| Postpartum nitric oxide, oxidants and antioxidants levels in ewes and their lambs.Crossref | GoogleScholarGoogle Scholar |
Travnicek J, Pisek L, Herzig I, Doucha J, Kvicala J, Kroupova V, Rodinova H (2007) Selenium content in the blood serum and urine of ewes receiving selenium-enriched unicellular alga Chlorella. Veterinarni Medicina 52, 42–48.
| Selenium content in the blood serum and urine of ewes receiving selenium-enriched unicellular alga Chlorella.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXjsVKrur4%3D&md5=48b266a439912d1e0cc65b66fdbad2a3CAS |
Witko-Sarsat V, Friedlander M, Nguyen Khoa T, Capeillère-Blandin C, Nguyen AT, Canteloup S (1998) Advanced oxidation protein products as a novel mediators of inflammation and monocyte activation in a chronic renal failure. Journal of Immunity 161, 2524–2532.
