Fluorescence Microscopy Study of Protein Adsorption at Modified Glassy Carbon Surfaces
Alison J. Downard A C , Sandra L. Jackson B and Emelyn S. Q. Tan AA MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Chemistry, University of Canterbury, Christchurch, New Zealand.
B School of Biological Sciences, University of Canterbury, Christchurch, New Zealand.
C Corresponding author. Email: alison.downard@canterbury.ac.nz
Australian Journal of Chemistry 58(4) 275-279 https://doi.org/10.1071/CH04259
Submitted: 29 October 2004 Accepted: 6 January 2005 Published: 5 April 2005
Abstract
Glassy carbon (GC) surfaces were modified with thin films by means of electrochemically assisted reduction of aryl diazonium salts and oxidation of primary amines. GC plates with modified and unmodified areas were exposed to solutions of fluorescently labelled bovine serum albumin (BSA-FITC). Fluorescence microscopy was found to be a simple and direct method for comparing adsorption of BSA-FITC between the different areas. Modification with methylbenzene, hexylbenzene, poly(ethylene glycol) benzene, and tetraethylene glycol diamine groups increases protein adsorption relative to unmodified GC. Hexylamine and the poly(ethylene glycol) diamine ED-2003 reduce protein adsorption. The results give insight into some factors controlling protein adsorption at these surface films.
Acknowledgments
The authors thank Dr Alan Happer for preparation of poly(ethylene glycol) benzene diazonium tetrafluoroborate and Mr Manfred Ingerfeld for assistance with fluorescence microscopy. The research was funded by the MacDiarmid Institute for Advanced Materials and Nanotechnology and the University of Canterbury. E.S.Q.T. thanks the MacDiarmid Institute for Scholarship funding.
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