N.M.R. determination of the sites of coordination of some paramagnetic metal ions with Adenosine-5'-monophosphate

Abstract

The ¹H and ³¹P magnetic resonance spectra of adenosine-5'-monophosphate (AMP) have been recorded as a function of pD in the presence and absence of low molar-ratios of the paramagnetic metal ions Mn²⁺, Ni²⁺, Co²⁺ Cr³⁺, CU²⁺, Fe²⁺, and Fe³⁺. All of these cations form chelate complexes involving the phosphate moiety and the adenine ring of AMP, but different binding sites on the adenine ring appear to be utilized.