Synthesis of the major encephalitogenic determinant of myelin basic protein and the ¹³C N.M.R. of some protected intermediates

Abstract

The main encephalitogenic determinant of bovine myelin basic protein (MBP 114-122) has been synthesized through fragment condensation of tetrapeptide, Boc-Phe-Ser-Trp-Gly-OH, and penta-peptide, H-Ala- Glu(OBzl)-Gly-Gln-Lys(N^ε-Cbz)-Obzl. It was observed that the benzyl ether protecting group of the serine side chain was not removed during catalytic hydrogenation (Pd/C-H₂) of the tetrapeptide, Boc-Phe- Ser(OBzl)-Trp-Gly-OBzl. ¹³C N.M.R. spectra of some other intermediates are discussed.