Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Biosolids differently affect seed yield, nodule growth, nodule-specific activity, and symbiotic nitrogen fixation of field bean

S. Pampana A , A. Scartazza B , R. Cardelli A , A. Saviozzi A , L. Guglielminetti A , G. Vannacci A , M. Mariotti C , A. Masoni A and I. Arduini A D
+ Author Affiliations
- Author Affiliations

A Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.

B Institute of Agro-environmental and Forest Biology, National Research Council of Italy (CNR), Via Salaria km 29, 300, I-00016 Monterotondo Scalo, RM, Italy.

C Department of Veterinary Science, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.

D Corresponding author. Email: iduna.arduini@unipi.it

Crop and Pasture Science 68(8) 735-745 https://doi.org/10.1071/CP17166
Submitted: 24 April 2017  Accepted: 2 August 2017   Published: 15 September 2017

Abstract

The main aim of this research was to verify whether mineral nitrogen (N) continuously released by organic fertilisers during the field bean growth cycle may be sufficiently high to enhance plant growth and seed yield but sufficiently low that it does not negatively affect nodulation and symbiotic N2 fixation. Plants were grown without N fertilisation, and with mineral and organic N (biosolids) fertilisation. All plant parts were collected and dry matter, N content, %Ndfa, and N2 fixed were measured at 8th node, flowering, and maturity stages. Nodule specific activity, N derived from soil, and N remobilisation were estimated. The nitrate concentration of soil was also determined. Biosolids reduced nodule growth, nodule fixation activity, and N2 fixation during the vegetative but not the reproductive phase. During seed filling, nodule fixation activity increased and N2 fixation was roughly twice that of the Control plants. Biosolids increased seed yield by removing the imbalance between N demand and N supply for pod growth. This may be related to an increase in nodule-specific activity due to the reduction in mineral N in the soil.

Additional keywords: nitrogen isotope composition, nitrogen remobilisation, %Ndfa.


References

Abd-Alla MH, Yan F, Schubert S (1999) Effects of sewage sludge application on nodulation, nitrogen fixation, and plant growth of faba bean, soybean, and lupin. Journal of Applied Botany 73, 69–75.

Antolín MC, Fiasconaro ML, Sánchez-Díaz M (2010) Relationship between photosynthetic capacity, nitrogen assimilation and nodule metabolism in alfalfa (Medicago sativa) grown with sewage sludge. Journal of Hazardous Materials 182, 210–216.
Relationship between photosynthetic capacity, nitrogen assimilation and nodule metabolism in alfalfa (Medicago sativa) grown with sewage sludge.CrossRef |

Arrese-Igor C, Minchin FR, Gordon AJ, Nath AK (1997) Possible causes of the physiological decline in soybean nitrogen fixation in the presence of nitrate. Journal of Experimental Botany 48, 905–913.
Possible causes of the physiological decline in soybean nitrogen fixation in the presence of nitrate.CrossRef |

Caliskan S, Ozkaya I, Caliskan ME, Arslan M (2008) The effects of nitrogen and iron fertilization on growth, yield and fertilizer use efficiency of soybean in a Mediterranean-type soil. Field Crops Research 108, 126–132.
The effects of nitrogen and iron fertilization on growth, yield and fertilizer use efficiency of soybean in a Mediterranean-type soil.CrossRef |

Cassman KG, Dobermann A, Walters DT, Yang H (2003) Meeting cereal demand while protecting natural resources and improving environmental quality. Annual Review of Environment and Resources 28, 315–358.
Meeting cereal demand while protecting natural resources and improving environmental quality.CrossRef |

Cheema ZA, Ahmad A (2000) Effects of urea on the nitrogen fixing capacity and growth of grain legumes. International Journal of Agriculture and Biology 2, 388–394.

Christie P, Easson DL, Picton JR, Love SCP (2001) Agronomic value of alkaline-stabilized sewage biosolids for spring barley. Agronomy Journal 93, 144–151.
Agronomic value of alkaline-stabilized sewage biosolids for spring barley.CrossRef | 1:CAS:528:DC%2BD3MXitl2mu7c%3D&md5=369d866dd1ea6e90db74855701f864b2CAS |

Crozat Y, Aveline A, Coste F, Gillet JP, Domenach AM (1994) Yield performance and seed production pattern of field‐grown pea and soybean in relation to N nutrition. European Journal of Agronomy 3, 135–144.
Yield performance and seed production pattern of field‐grown pea and soybean in relation to N nutrition.CrossRef | 1:CAS:528:DyaK2cXlsFKmsrk%3D&md5=176064dd0ef252a7c898d21946f93952CAS |

Davies SN, Turner NC, Palta JA, Siddique KHM, Plummer JA (2000) Remobilisation of carbon and nitrogen supports seed filling in chickpea subjected to water deficit. Australian Journal of Agricultural Research 51, 855–866.
Remobilisation of carbon and nitrogen supports seed filling in chickpea subjected to water deficit.CrossRef |

Eaglesham ARJ, Hassouna S, Seegers R (1983) Fertilizer-N effects on N2 fixation by cowpea and soybean. Agronomy Journal 75, 61–66.
Fertilizer-N effects on N2 fixation by cowpea and soybean.CrossRef |

Egli DB (2005) Flowering, pod set and reproductive success in soya bean. Journal of Agronomy & Crop Science 191, 283–291.
Flowering, pod set and reproductive success in soya bean.CrossRef |

Egli DB, Bruening WP (2007) Nitrogen accumulation and redistribution in soybean genotypes with variation in seed protein concentration. Plant and Soil 301, 165–172.
Nitrogen accumulation and redistribution in soybean genotypes with variation in seed protein concentration.CrossRef | 1:CAS:528:DC%2BD1cXosVWkug%3D%3D&md5=2f05a4e75845726cbb2cf2218ecb9e24CAS |

Esperschuetz J, Bulman S, Anderson C, Lense O, Horswell J, Dickinson N, Robinson BH (2016) Production of biomass crops using biowastes on low-fertility soil: 2. Effect of biowastes on nitrogen transformation processes. Journal of Environmental Quality 45, 1970–1978.
Production of biomass crops using biowastes on low-fertility soil: 2. Effect of biowastes on nitrogen transformation processes.CrossRef | 1:CAS:528:DC%2BC2sXksVKlt7g%3D&md5=4d973699df89c3d6a6115f6cee2456d9CAS |

Fujikake H, Yashima H, Sato T, Ohtake N, Sueyoshi K (2002) Rapid and reversible nitrate inhibition of nodule growth and N2 fixation activity in soybean (Glycine max (L.) Merr.). Soil Science and Plant Nutrition 48, 211–217.
Rapid and reversible nitrate inhibition of nodule growth and N2 fixation activity in soybean (Glycine max (L.) Merr.).CrossRef | 1:CAS:528:DC%2BD38XjtFyqtrY%3D&md5=da850faa08c2ff44cb1ab5a30d03b5b4CAS |

Fujikake H, Yamazaki A, Ohtake N, Sueyoshi K, Matsuhashi S, Ito T, Mizuniwa C, Kume T, Hashimoto S, Ishioka NS, Watanabe S, Osa A, Sekine T, Uchida H, Tsuji A, Ohyama T (2003) Quick and reversible inhibition of soybean root nodule growth by nitrate involves a decrease in sucrose supply to nodules. Journal of Experimental Botany 54, 1379–1388.
Quick and reversible inhibition of soybean root nodule growth by nitrate involves a decrease in sucrose supply to nodules.CrossRef | 1:CAS:528:DC%2BD3sXjt1OgsL8%3D&md5=bed226fe74063dc5a011a385e7be7f8dCAS |

Gan Y, Stulen I, Posthumus F, van Keulen H, Kuiper PJC (2002) Effects of N management on growth, N fixation and yield of soybean. Nutrient Cycling in Agroecosystems 62, 163–174.
Effects of N management on growth, N fixation and yield of soybean.CrossRef | 1:CAS:528:DC%2BD38XlsVyitrc%3D&md5=389cd009ff40b769cc60eb0aacfe6a95CAS |

Knott CM (1990) A key for stages of development of the faba bean (Vicia faba). Annals of Applied Biology 116, 391–404.
A key for stages of development of the faba bean (Vicia faba).CrossRef |

Kocon A (2010) The effect of foliar or soil top-dressing of urea on some physiological processes and seed yield of faba bean. Polish Journal of Agronomy 3, 15–19.

Kumar K, Goh KM (2000) Biological nitrogen fixation, accumulation of soil nitrogen and nitrogen balance for white clover (Trifolium repens L.) and field pea (Pisum sativum L.) grown for seed. Field Crops Research 68, 49–59.
Biological nitrogen fixation, accumulation of soil nitrogen and nitrogen balance for white clover (Trifolium repens L.) and field pea (Pisum sativum L.) grown for seed.CrossRef |

Kumarasinghe KS, Danso SKA, Zapata F (1992) Field evaluation of fixation and N partitioning in climbing bean (Phaseolus vulgaris L.) using 15N. Biology and Fertility of Soils 13, 142–146.

López-Bellido FJ, López-Bellido L, López-Bellido RJ (2005) Competition, growth and yield of faba bean (Vicia faba L.). European Journal of Agronomy 23, 359–378.
Competition, growth and yield of faba bean (Vicia faba L.).CrossRef |

Lu Q, He ZL, Stoffella PJ (2012) Land application of biosolids in the USA: A review. Applied and Environmental Soil Science 2012, 201462
Land application of biosolids in the USA: A review.CrossRef |

Mantovi P, Baldoni G, Toderi G (2005) Reuse of liquid, dewatered, and composted sewage sludge on agricultural land: effects of long-term application on soil and crop. Water Research 39, 289–296.
Reuse of liquid, dewatered, and composted sewage sludge on agricultural land: effects of long-term application on soil and crop.CrossRef | 1:CAS:528:DC%2BD2MXisFChsQ%3D%3D&md5=d569dceb91385a59fae59a45f8d5aef3CAS |

Munn KJ, Evans J, Chalk PM (2001) Nitrogen fixation characteristics of Rhizobium surviving in soils “equilibrated” with sewage biosolids. Australian Journal of Agricultural Research 52, 963–972.
Nitrogen fixation characteristics of Rhizobium surviving in soils “equilibrated” with sewage biosolids.CrossRef |

Namvar A, Sharifi RS, Khandan T (2011) Growth analysis and yield of chickpea (Cicer arietinum L.) in relation to organic and inorganic nitrogen fertilization. Ekologia 57, 97–108.

Nanjareddy K, Blanco L, Arthikala MK, Affantrange XA, Sánchez F, Lara M (2014) Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.). Journal of Integrative Plant Biology 56, 281–298.
Nitrate regulates rhizobial and mycorrhizal symbiosis in common bean (Phaseolus vulgaris L.).CrossRef | 1:CAS:528:DC%2BC2cXktlOitb0%3D&md5=1ad71ef0421217bfe176fa6c87333f58CAS |

Naudin C, Corre-Hellou G, Voisin AS, Oury V, Salon C, Crozat Y, Jeuffroy MH (2011) Inhibition and recovery of symbiotic N2 fixation by peas (Pisum sativum L.) in response to short-term nitrate exposure. Plant and Soil 346, 275–287.
Inhibition and recovery of symbiotic N2 fixation by peas (Pisum sativum L.) in response to short-term nitrate exposure.CrossRef | 1:CAS:528:DC%2BC3MXhtValtbrE&md5=9950288794587f85ff3e6049022e8d6bCAS |

Nebiyu A, Huygens D, Ram Upadhayay H, Diels J, Boeckx P (2014) Importance of correct B value determination to quantified biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance. Biology and Fertility of Soils 50, 517–525.
Importance of correct B value determination to quantified biological N2 fixation and N balances of faba beans (Vicia faba L.) via 15N natural abundance.CrossRef | 1:CAS:528:DC%2BC2cXktlerurk%3D&md5=e8d6f3b31a8402579f2c507ab902d4e8CAS |

Pampana S, Masoni A, Arduini I (2016) Grain legumes differ in nitrogen accumulation and remobilisation during seed filling. Acta Agriculturae Scandinavica, Section B – Soil and Plant Science 66, 127–132.

Pampana S, Masoni A, Mariotti M, Ercoli L, Arduini I (2017) Nitrogen fixation of grain legumes differs in response to nitrogen fertilisation. Experimental Agriculture
Nitrogen fixation of grain legumes differs in response to nitrogen fertilisation.CrossRef | In press.

Rennie RJ, Kemp GA (1984) 15N-determined time course for N2 fixation in two cultivars of field bean. Agronomy Journal 76, 146–154.
15N-determined time course for N2 fixation in two cultivars of field bean.CrossRef | 1:CAS:528:DyaL2cXhtFOru7s%3D&md5=d09a6bb87b9c6b3ded8d98ebb0140559CAS |

Rigby H, Clarke BO, Pritchard DL, Meehan B, Beshah F, Smith SR, Porter NA (2016) A critical review of nitrogen mineralization in biosolids-amended soil, the associated fertilizer value for crop production and potential for emissions to the environment. The Science of the Total Environment 541, 1310–1338.
A critical review of nitrogen mineralization in biosolids-amended soil, the associated fertilizer value for crop production and potential for emissions to the environment.CrossRef | 1:CAS:528:DC%2BC2MXhs1OntLzF&md5=e562105e1cdd82f916140fb82509525dCAS |

Salon C, Munier-Jolain NG, Duc G, Voisin AS, Grandgirard D, Larmure A, Emery RJN, Ney B (2001) Grain legume seed filling in relation to nitrogen acquisition: A review and prospects with particular reference to pea. Agronomie 21, 539–552.
Grain legume seed filling in relation to nitrogen acquisition: A review and prospects with particular reference to pea.CrossRef |

Salvagiotti F, Cassman KG, Specht JE, Walters DT, Weiss A, Dobermann A (2008) Nitrogen uptake, fixation and response to fertilizer N in soybeans: A review. Field Crops Research 108, 1–13.
Nitrogen uptake, fixation and response to fertilizer N in soybeans: A review.CrossRef |

Schiltz S, Munier-Jolain N, Jeudy C, Burstin J, Salon C (2005) Dynamics of exogenous nitrogen remobilization from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling. Plant Physiology 137, 1463–1473.
Dynamics of exogenous nitrogen remobilization from vegetative organs in pea revealed by 15N in vivo labeling throughout seed filling.CrossRef | 1:CAS:528:DC%2BD2MXjslaqu7c%3D&md5=7384e4a3c88d61b5a081f80842ca4857CAS |

Steel RGD, Torrie JH, Dickey DA (1997) ‘Principles and procedures of statistics: a biometrical approach.’ (McGraw-Hill: New York)

Streeter J (1988) Inhibition of legume nodule formation and N2 fixation by nitrate. Critical Reviews in Plant Sciences 7, 1–23.
Inhibition of legume nodule formation and N2 fixation by nitrate.CrossRef | 1:CAS:528:DyaL1MXnt1CgtQ%3D%3D&md5=c0fb693bb2ad26be5902a721b692b373CAS |

Sullivan DM, Bary AI, Cogger CG, Shearin TE (2009) Predicting biosolids application rates for dryland wheat across a range of Northwest climate zones. Communications in Soil Science and Plant Analysis 40, 1770–1789.
Predicting biosolids application rates for dryland wheat across a range of Northwest climate zones.CrossRef | 1:CAS:528:DC%2BD1MXotFGqtb8%3D&md5=a8d09ca3dfb6cbc0bb3e7a2dcac30d0fCAS |

Thies JE, Singleton PW, Bohlool BB (1995) Phenology, growth, and yield of field-grown soybean and bush bean as a function of varying modes of N nutrition. Soil Biology & Biochemistry 27, 575–583.
Phenology, growth, and yield of field-grown soybean and bush bean as a function of varying modes of N nutrition.CrossRef | 1:CAS:528:DyaK2MXlt12rtro%3D&md5=5aa8741afdb22d37fc07a0184378e83bCAS |

Unkovich M, Herridge D, Peoples M, Cadisch G, Boddey R, Giller K, Alves B, Chalk P (2008) ‘Measuring plant-associated nitrogen fixation in agricultural systems.’ ACIAR Monograph No. 136. (ACIAR: Canberra)

Voisin AS, Salon C, Munier-Jolain NG, Ney B (2002a) Effect of mineral nitrogen on nitrogen nutrition and biomass partitioning between the shoot and roots of pea (Pisum sativum L.). Plant and Soil 242, 251–262.
Effect of mineral nitrogen on nitrogen nutrition and biomass partitioning between the shoot and roots of pea (Pisum sativum L.).CrossRef | 1:CAS:528:DC%2BD38XltFKisbg%3D&md5=e7930b24033c243feaa3ba1efaa68d0bCAS |

Voisin AS, Salon C, Munier-Jolain NG, Ney B (2002b) Quantitative effect of soil nitrate, growth potential and phenology on symbiotic nitrogen fixation of pea (Pisum sativum L.). Plant and Soil 243, 31–42.
Quantitative effect of soil nitrate, growth potential and phenology on symbiotic nitrogen fixation of pea (Pisum sativum L.).CrossRef | 1:CAS:528:DC%2BD38XmsFWmur4%3D&md5=ee64bd820dadc335cab7f3ed133070dcCAS |



Rent Article (via Deepdyve) Export Citation