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Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

A cytological and molecular analysis of D-genome chromosome retention following F2–F6 generations of hexaploid × tetraploid wheat crosses

Sriram Padmanaban A , Peng Zhang B , Mark W. Sutherland A , Noel L. Knight A C and Anke Martin A D
+ Author Affiliations
- Author Affiliations

A Centre for Crop Health, University of Southern Queensland, Toowoomba, Qld 4350, Australia.

B Plant Breeding Institute, The University of Sydney, Cobbitty, NSW 2570, Australia.

C Present address: School of Integrative Plant Science, Plant Pathology and Plant–Microbe Biology Section, Cornell University, Geneva, NY 14456, USA.

D Corresponding author. Email: anke.martin@usq.edu.au

Crop and Pasture Science 69(2) 121-130 https://doi.org/10.1071/CP17240
Submitted: 7 July 2017  Accepted: 26 November 2017   Published: 5 February 2018

Abstract

Both hexaploid bread wheat (AABBDD) (Triticum aestivum L.) and tetraploid durum wheat (AABB) (T. turgidum spp. durum) are highly significant global food crops. Crossing these two wheats with different ploidy levels results in pentaploid (AABBD) F1 lines. This study investigated the differences in the retention of D chromosomes between different hexaploid × tetraploid crosses in subsequent generations by using molecular and cytological techniques. Significant differences (P < 0.05) were observed in the retention of D chromosomes in the F2 generation depending on the parents of the original cross. One of the crosses, 2WE25 × 950329, retained at least one copy of each D chromosome in 48% of its F2 lines. For this cross, the retention or elimination of D chromosomes was determined through several subsequent self-fertilised generations. Cytological analysis indicated that D chromosomes were still being eliminated at the F5 generation, suggesting that in some hexaploid × tetraploid crosses, D chromosomes are unstable for many generations. This study provides information on the variation in D chromosome retention in different hexaploid × tetraploid wheat crosses and suggests efficient strategies for utilising D genome retention or elimination to improve bread and durum wheat, respectively.

Additional keywords: DArTseq, FISH, GISH, interploidy crosses.


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