Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality

Just Accepted

This article has been peer reviewed and accepted for publication. It is in production and has not been edited, so may differ from the final published form.

A cytological and molecular analysis of D-genome chromosome retention in the F2 to F6 generation of hexaploid/tetraploid wheat crosses

Sriram Padmanaban , Peng Zhang , Mark Sutherland , Noel Knight , Anke Martin

Abstract

Both hexaploid bread wheat AABBDD (Triticum aestivum L) and tetraploid durum wheat (AABB) (T. turgidum spp. durum) are highly significant global food crops. Crossing these two wheats with different ploidy levels results in pentaploid (AABBD) F1 lines. This study investigated the differences in the retention of D chromosomes between different hexaploid/tetraploid crosses in subsequent generations using molecular and cytological techniques. Significant differences (P<0.05) were observed in the retention of D chromosomes in the F2 generation depending on the parents of the original cross. One of the crosses, 2WE25/950329, retained at least one copy of each D chromosome in 48% of its F2 lines. For this cross the retention or elimination of D chromosomes was determined through a number of subsequent self-fertilised generations. Cytological analysis results indicated that D chromosomes were still being eliminated at the F5 generation suggesting that in some hexaploid/tetraploid crosses D chromosomes are unstable for many generations. This study provides information on the variation in D chromosome retention in different hexaploid/tetraploid wheat crosses and suggests efficient strategies for utilising D genome retention or elimination to improve bread and durum wheat respectively.

CP17240  Accepted 26 November 2017

© CSIRO 2017