Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality

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Molecular and Phenotypic Diversity among Chickpea (Cicer arietinum L.) genotypes as a function of drought tolerance

C Bharadwaj , SUPRIYA SACHDEVA , Vinay Sharma , B Patil , Khela Soren , Manish Roorkiwal , Rajeev Varshney , K Bhat


Diversity as a function of drought tolerance could be identified by morphological characters and the molecular tools could find the most divergent genotypes for breeding programmes for drought tolerance in future. The narrow genetic base of chickpea can be circumvented by using diverse lines in breeding programmes. Forty chickpea genotypes were studied for their morphological and molecular diversity with an objective of identifying the most diverse drought tolerant lines. A total of 90 alleles were detected with 3.6 alleles per locus. PIC values ranged from 0.155 to 0.782 with an average value of 0.4374 per locus. The size of amplified products ranged from 160 bp to 390 bp. Primer TA136 with 8 alleles showed the highest PIC of 0.7825 inidcating its ability to differentiate the genotypes much better at molecular level. DARwin neighbour joining tree analysis based on dissimilarity estimates was done for the molecular and Sequential agglomerative hierarchial non-overlapping (SAHN) grouping for morphological data. It could clearly discriminate the tolerance and the sensitivity of genotypes. The two dimensional PCoA plot indicated good diversity for drought tolerance. The genetic similarity coefficients ranged from 0.115 (BGD72 to ICCV 5308) to 0.828 (ICCV 10316 to ICCV 92337).

CP17284  Accepted 22 November 2017

© CSIRO 2017