Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality

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In vitro regeneration from leaf-base segments in three genotypes of Urochloa spp

Diliane Yaguinuma , Luciana Takamori , Adriana Oliveira , Luiz Gonzaga Vieira , Alessandra Ribas


We evaluated the regenerative potential from three commercial genotypes of Urochloa sp P. Beauv. (U. brizantha cv. Marandu; U. decumbens cv. Basilisk and U. ruziziensis cv. Ruziziensis) using leaf-base segments as explants. We tested two auxins (2,4-D and picloram) and one cytokinin (TDZ) in four concentrations (1, 2, 3 and 4 mg L-1). Seeds were scarified, peeled and disinfected prior to inoculation on half strength MS media in the dark for 14 days. Leaf-base explants were sectioned in thin slices and inoculated into the media. We analyzed the number of primary calluses, number of calluses with shoots clusters and the average of regenerated plants. The lowest concentration of auxins tested (1 mg L-1) yielded the highest number of regenerated plants for MarandĂș and Basilisk, while the optimum for Ruziziensis was 2 mg L-1. Medium with higher concentrations of TDZ (4 mg L-1) was required to produce high frequency of plants for all genotypes. Explants cultured on media with TDZ produced very few calluses. These results indicate that the auxins and the cytokinin tested can induce plant regeneration from Urochloa leaf-base segments, and may be used to produce transgenic plants in genetic transformation studies.

CP17395  Accepted 19 February 2018

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