Monitoring programmed cell death triggered by mild heat shock in soybean-cultured cells
Anna Zuppini A B , Valentina Bugno A and Barbara Baldan AA Dipartimento di Biologia, Università di Padova, via U. Bassi 58 / B, 35131 Padova, Italy.
B Corresponding author. Email: zuppini@civ.bio.unipd.it
Functional Plant Biology 33(7) 617-627 https://doi.org/10.1071/FP06015
Submitted: 17 January 2006 Accepted: 29 March 2006 Published: 3 July 2006
Abstract
Programmed cell death (PCD) is a common form of cellular demise during plant response to environmental stresses. The pathway of PCD has been partially clarified in plants although the underlying molecular mechanisms are still poorly defined. We have investigated the signalling cascade induced by a mild heat treatment causing PCD in soybean cells (Glycine max L.). The data show that heat shock led to the onset of PCD in soybean cells involving H2O2 production and mitochondrial damage. Cytochrome c release accompanies the presence of caspase 9-like and caspase 3-like protease activities. Concomitantly, cells were severely damaged with a progressive cell shrinkage, chloroplast alteration and detachment of the plasma membrane from the cell wall. Chromatin condensation and DNA damage were observed. It is proposed that a mild heat stress induces PCD in soybean cells through a caspase-like-dependent pathway.
Keywords: caspase-like activity, heat shock, mitochondria, programmed cell death, soybean cells.
Acknowledgments
We thank G Neuhaus (Freiburg, Germany) for kindly providing the soybean suspension cells (SB-P), and A Vitale (Milano, Italy) for the kind gift of the tobacco anti-BiP antibody. The authors are grateful to P Mariani and L Navazio (Dipartimento di Biologia, Padova, Italy) for fruitful suggestions and discussion. This work was supported by funds from the University of Padova (to BB) and by grants from PRIN (prot. 2004052535_003).
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