Exogenous abscisic acid reduces water loss and improves antioxidant defence, desiccation tolerance and transpiration efficiency in two spring wheat cultivars subjected to a soil water deficit
Yan-Lei Du A , Zhen-Yu Wang A , Jing-Wei Fan A , Neil C. Turner A B C , Jin He A , Tao Wang A and Feng-Min Li A B D
+ Author Affiliations
- Author Affiliations
A State Key Laboratory of Grassland Agro-ecosystems, Institute of Arid Agroecology, School of Life Sciences, Lanzhou University, Lanzhou 730 000, Gansu Province, China.
B The UWA Institute of Agriculture, M082, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C Centre for Legumes in Mediterranean Agriculture, M080, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
D Corresponding author. Email: fmli@lzu.edu.cn
Functional Plant Biology 40(5) 494-506 https://doi.org/10.1071/FP12250
Submitted: 29 August 2012 Accepted: 14 January 2013 Published: 18 February 2013
Abstract
The effect of soil drenching with 10 µM abscisic acid (ABA) on the physiological responses of two spring wheat (Triticum aestivum L.) cultivars released in different decades was evaluated when subjected to a water deficit at jointing or at booting. Exogenous ABA application increased the ABA concentration in the leaves, reduced the stomatal conductance (gs), slowed the rate of water use, decreased the lethal leaf water potential (ψ) used to measure desiccation tolerance and lowered the soil water content (SWC) at which leaf relative water content (RWC) began to decrease and wilting was observed. Exogenous ABA application also reduced reactive oxygen species (ROS) formation and increased antioxidant enzyme activity, leading to a reduction in the oxidative damage to lipid membranes in both cultivars exposed to water stress at jointing and booting. The decrease in leaf RWC and wilting occurred at lower values of SWC in the recently-released cultivar than in the earlier-released cultivar. The recently-released cultivar also had higher grain yield than the earlier-released cultivar at moderate water stress, but the grain yield in both cultivars was reduced by water stress and by the exogenous ABA treatment. However, exogenous ABA treatment increased transpiration efficiency for grain (TEG) of both cultivars under moderate water stress. These results indicate that ABA played an important role in slowing water use and enhancing the antioxidant defence during soil drying, but this did not result in increased yields under drought stress.
Additional keywords: antioxidant enzymes, drought stress, reactive oxygen species (ROS), Triticum aestivum, water use efficiency.
References
Aasamaa K, Sõber A, Hartung W, Niinemets Ü (2004) Drought acclimation of two deciduous tree species of different layers in a temperate forest canopy. Trees – Structure and Function 18, 93–101.| Drought acclimation of two deciduous tree species of different layers in a temperate forest canopy.Crossref | GoogleScholarGoogle Scholar |
Aebi H (1984) Catalase in vitro. In ‘Methods in enzymology’. (Ed. L Packer) pp. 121–126. (Academic Press: London)
Amako K, Chen GX, Asada K (1994) Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant & Cell Physiology 35, 497–504.
Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55, 373–399.
| Reactive oxygen species: metabolism, oxidative stress, and signal transduction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFeisL0%3D&md5=bb62c10883f0f45b9778cbbe05a12a70CAS |
Augé RM, Duan X, Croker JL, Witte WT, Green CD (1998) Foliar dehydration tolerance of twelve deciduous tree species. Journal of Experimental Botany 49, 753–759.
Bollmark M, Kubat B, Eliasson L (1988) Variation in endogenous cytokinin content during adventitious root formation in pea cuttings. Journal of Plant Physiology 132, 262–265.
| Variation in endogenous cytokinin content during adventitious root formation in pea cuttings.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXktlGgsLY%3D&md5=a38a2b9868422e73b515fb588e0ca758CAS |
Brennan T, Frekel C (1977) Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiology 59, 411–416.
| Involvement of hydrogen peroxide in the regulation of senescence in pear.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXhs1yjsL4%3D&md5=06786b7a9f4334741b907a8dc6d10bc7CAS |
Davies WJ, Zhang J (1991) Root signals and the regulation of growth and development of plants in drying soil. Annual Review of Plant Biology 42, 55–76.
| Root signals and the regulation of growth and development of plants in drying soil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXltFSmsr8%3D&md5=91a7fd4a63e9eb54599c96211ca9e0d2CAS |
Davies WJ, Kudoyarova G, Hartung W (2005) Long-distance ABA signaling and its relation to other signaling pathways in the detection of soil drying and the mediation of the plant’s response to drought. Journal of Plant Growth Regulation 24, 285–295.
| Long-distance ABA signaling and its relation to other signaling pathways in the detection of soil drying and the mediation of the plant’s response to drought.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXhtlGhurbI&md5=30f8e2c23bac5503bfbe11ab1022bc0eCAS |
Du YL, Wang ZY, Fan JW, Turner NC, Wang T, Li FM (2012) β-Aminobutyric acid increases abscisic acid accumulation and desiccation tolerance and decreases water use but fails to improve grain yield in two spring wheat cultivars under soil drying. Journal of Experimental Botany 63, 4849–4860.
| β-Aminobutyric acid increases abscisic acid accumulation and desiccation tolerance and decreases water use but fails to improve grain yield in two spring wheat cultivars under soil drying.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xht1KjsLfF&md5=f5205e687c2bee414343e80a092a8714CAS |
Duan B, Yang Y, Lu Y, Korpelainen H, Berninger F, Li C (2007) Interactions between water deficit, ABA, and provenances in Picea asperata. Journal of Experimental Botany 58, 3025–3036.
| Interactions between water deficit, ABA, and provenances in Picea asperata.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhtFWitLjN&md5=21d5a8660532537bb9a283199520a091CAS |
Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase. Analytical Biochemistry 70, 616–620.
| Inhibition of nitrite formation from hydroxylammoniumchloride: a simple assay for superoxide dismutase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE28XhtFOnt7s%3D&md5=10e78610830e862db9fc5c5099366938CAS |
Fan XW, Li FM, Song L, Xiong YC, An LZ, Jia Y, Fang XW (2009) Defense strategy of old and modern spring wheat varieties during soil drying. Physiologia Plantarum 136, 310–323.
| Defense strategy of old and modern spring wheat varieties during soil drying.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXos1artb4%3D&md5=82d07c0aa789e92b39fbbfdea150d120CAS |
Giannopolitis CN, Ries SK (1977) Superoxide dismutases I. Occurrence in higher plants. Plant Physiology 59, 309–314.
| Superoxide dismutases I. Occurrence in higher plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXhtlKgtrs%3D&md5=2af9706635b5030196b2fde0c41ce548CAS |
Halliwell B, Gutteridge JMC (1999) ‘Free radicals in biology and medicine.’ (Oxford University Press: New York)
Hartung W, Sauter A, Turner NC, Fillery I, Heilmeier H (1996) Abscisic acid in soils: what is its function and which factors and mechanisms influence its concentration? Plant and Soil 184, 105–110.
| Abscisic acid in soils: what is its function and which factors and mechanisms influence its concentration?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXlsVOruw%3D%3D&md5=d3da1c61302899053bbd837fe8e4f779CAS |
Hose E, Steudle E, Hartung W (2000) Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes. Planta 211, 874–882.
| Abscisic acid and hydraulic conductivity of maize roots: a study using cell- and root-pressure probes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXotVKnt7k%3D&md5=9a2d73f7ad8b7deddd1b42d30bbabc5eCAS |
Jiang M, Zhang J (2001) Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant & Cell Physiology 42, 1265–1273.
| Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXos1Oms74%3D&md5=4cda21ce711d4ef195cdfa3fcc6aa465CAS |
Jiang M, Zhang J (2002) Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. Journal of Experimental Botany 53, 2401–2410.
| Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhtVKgsg%3D%3D&md5=47fc8df742f23b7e7709743fd75815caCAS |
Jones MM, Turner NC (1980) Osmotic adjustment in expanding and fully expanded leaves of sunflower in response to water deficits. Australian Journal of Plant Physiology 7, 181–192.
| Osmotic adjustment in expanding and fully expanded leaves of sunflower in response to water deficits.Crossref | GoogleScholarGoogle Scholar |
Li C, Yin C, Liu S (2004) Different responses of two contrasting Populus davidiana populations to exogenous abscisic acid application. Environmental and Experimental Botany 51, 237–246.
| Different responses of two contrasting Populus davidiana populations to exogenous abscisic acid application.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXjs1ertbg%3D&md5=4ceb6c3e2f1ea98ef254ae74cd1cfcbaCAS |
Ozturk ZN, Talamé V, Deyholos M, Michalowski CB, Galbraith DW, Gozukirmizi N, Tuberosa R, Bohnert HJ (2002) Monitoring large-scale changes in transcript abundance in drought-and salt-stressed barley. Plant Molecular Biology 48, 551–573.
| Monitoring large-scale changes in transcript abundance in drought-and salt-stressed barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjsFWrtr0%3D&md5=8741a097063fd34ab28d32c218e7dce9CAS |
Sauter A, Davies WJ, Hartung W (2001) The long-distance abscisic acid signal in the droughted plant: the fate of the hormone on its way from root to shoot. Journal of Experimental Botany 52, 1991–1997.
| The long-distance abscisic acid signal in the droughted plant: the fate of the hormone on its way from root to shoot.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXns1Wkt78%3D&md5=86b3afccbf1b0cb8f456cb4b6129e56aCAS |
Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiology 59, 1011–1012.
| Chloroplast glutathione reductase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaE2sXktlCqs7c%3D&md5=2c19f8cb046c976228c74fda464a1c84CAS |
Slovik S, Hartung W (1992) Compartmental distribution and redistribution of abscisic acid in intact leaves. III. Analysis of the stress-signal chain. Planta 187, 37–47.
| Compartmental distribution and redistribution of abscisic acid in intact leaves. III. Analysis of the stress-signal chain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK38XisFaiu70%3D&md5=59e04c6d0f8d232c2ab44bbbfc9b3e69CAS |
Tardieu F, Parent B, Simonneau T (2010) Control of leaf growth by abscisic acid: hydraulic or non-hydraulic processes? Plant, Cell & Environment 33, 636–647.
| Control of leaf growth by abscisic acid: hydraulic or non-hydraulic processes?Crossref | GoogleScholarGoogle Scholar |
Travaglia C, Reinoso H, Cohen A, Luna C, Tommasino E, Castillo C, Bottini R (2010) Exogenous ABA increases yield in field-grown wheat with moderate water restriction. Journal of Plant Growth Regulation 29, 366–374.
| Exogenous ABA increases yield in field-grown wheat with moderate water restriction.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXptVahsb4%3D&md5=e97237a88473e488e650c9acc8586647CAS |
Turner NC (1988) Measurement of plant water status by the pressure chamber technique. Irrigation Science 9, 289–308.
| Measurement of plant water status by the pressure chamber technique.Crossref | GoogleScholarGoogle Scholar |
Turner NC (1996) Further progress in crop water relations. Advances in Agronomy 58, 293–338.
| Further progress in crop water relations.Crossref | GoogleScholarGoogle Scholar |
Turner NC, Hartung W (2012) Dehydration of isolated roots of seven Lupinus species induces synthesis of different amounts of free, but not conjugated, abscisic acid. Journal of Plant Growth Regulation 66, 265–269.
| Dehydration of isolated roots of seven Lupinus species induces synthesis of different amounts of free, but not conjugated, abscisic acid.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XktVGnsbY%3D&md5=f547b7a29f21951be2e4e91c2db8c5dfCAS |
Turner NC, Meyer R (2011) Synthesis of regional impacts and global agricultural adjustments. In ‘Crop adaptation to climate change’. (Eds SS Yadav, RJ Redden, JL Hatfield, H Lotze-Campen, AE Hall) pp. 156–165. (Wiley-Blackwell: Chichester, UK)
Wilkinson S, Davies WJ (2010) Drought, ozone, ABA and ethylene: new insights from cell to plant to community. Plant, Cell & Environment 33, 510–525.
| Drought, ozone, ABA and ethylene: new insights from cell to plant to community.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXltV2hurs%3D&md5=6dcfef6bb2ff4576da06303500a9f758CAS |
Xiong YC, Li FM, Xu BC, Hodgkinson KC (2006) Hydraulic and non-hydraulic root-sourced signals in old and modern spring wheat cultivars in a semiarid area. Journal of Plant Growth Regulation 25, 120–136.
| Hydraulic and non-hydraulic root-sourced signals in old and modern spring wheat cultivars in a semiarid area.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmsVSjsLw%3D&md5=50b36ace2ea371af6378b2813fc80e7aCAS |
Yang J, Zhang J, Wang Z, Zhu Q, Wang W (2001) Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology 127, 315–323.
| Hormonal changes in the grains of rice subjected to water stress during grain filling.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXmvFCrtb8%3D&md5=bb8ff74980ff34f809c46c1919204b1aCAS |
Zhang J, Davies W (1990) Changes in the concentration of ABA in xylem sap as a function of changing soil water status can account for changes in leaf conductance and growth. Plant, Cell & Environment 13, 277–285.
| Changes in the concentration of ABA in xylem sap as a function of changing soil water status can account for changes in leaf conductance and growth.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3MXitFWmsQ%3D%3D&md5=47257e6383075efd0da26cb813abe090CAS |
Zhao SJ, Xu C, Zou Q, Meng Q (1994) Improvements of method for measurement of malondialdehyde in plant tissues. Plant Physiology Communications 30, 207–210.
