Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Genetic suppression of plant development and chloroplast biogenesis via the Snowy Cotyledon 3 and Phytochrome B pathways

Diep Ganguly A , Peter Crisp A , Klaus Harter B , Barry J. Pogson A and Verónica Albrecht-Borth A C
+ Author Affiliations
- Author Affiliations

A ARC (Australian Research Council) Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University Canberra, Acton, ACT 0200, Australia.

B Zentrum für Molekularbiologie der Pflanzen, Plant Physiology, University of Tübingen, 72076 Tübingen, Germany.

C Corresponding author. Email: veronica.albrecht@anu.edu.au

Functional Plant Biology 42(7) 676-686 https://doi.org/10.1071/FP15026
Submitted: 2 February 2015  Accepted: 2 April 2015   Published: 18 May 2015

Abstract

Plant development is regulated by external and internal factors such as light and chloroplast development. A revertant of the Arabidopsis thaliana (L.) Heyhn. chloroplast biogenesis mutant snowy cotyledon 3 (sco31) was isolated partially recovering the impaired chloroplast phenotype. The mutation was identified in the Phytochrome B (PhyB) gene and is a result of an amino acid change within the PAS repeat domain required for light-induced nuclear localisation. An independent phyB-9 mutation was crossed into sco31 mutants, resulting in the same partial reversion of sco31. Further analysis demonstrated that SCO3 and PhyB influence the greening process of seedlings and rosette leaves, embryogenesis, rosette formation and flowering. Interestingly, the functions of these proteins are interwoven in various ways, suggesting a complex genetic interaction. Whole-transcriptome profiling of sco31phyB-9 indicated that a completely distinct set of genes was differentially regulated in the double mutant compared with the single sco31 or phyB-9 mutants. Thus, we hypothesise that PhyB and SCO3 genetically suppress each other in plant and chloroplast development.

Additional keywords: Arabidopsis thaliana, chloroplast development, gene regulation phytochrome interacting factor 4.


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