Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy

Elena V. Tyutereva A , Ksenia S. Dobryakova A , Andreas Schiermeyer B , Maria F. Shishova C , Katharina Pawlowski D , Vadim Demidchik A E , Sigrun Reumann F G H and Olga V. Voitsekhovskaja A F I
+ Author Affiliations
- Author Affiliations

A Laboratory of Molecular and Ecological Physiology, Komarov Botanical Institute, Russian Academy of Sciences, ul. Professora Popova 2, 197376 Saint Petersburg, Russia.

B Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Department of Plant Biotechnology, Forckenbeckstrasse 6, D-52074 Aachen, Germany.

C Department of Physiology and Biochemistry of Plants, Saint Petersburg State University, Universitetskaya em., 7/9, 199034 Saint Petersburg, Russia.

D Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91 Stockholm, Sweden.

E Department of Plant Cell Biology and Bioengineering, Belarusian State University, Independence Avenue 4, 220030 Minsk, Belarus.

F Department of Plant Biochemistry, Albrecht-von-Haller-Institute for Plant Sciences, Georg-August-University of Goettingen, Justus-von-Liebig Weg 11, D-37077, Goettingen, Germany.

G Faculty of Science and Technology, Centre for Organelle Research (CORE), University of Stavanger, N-4036 Stavanger, Norway.

H Plant Biochemistry and Infection Biology, Universität Hamburg, D-22609 Hamburg, Germany.

I Corresponding author. Email: ovoitse@binran.ru

This paper originates from a presentation at the Fourth International Symposium on Plant Signaling and Behavior, Komarov Botanical Institute RAS/Russian Science Foundation, Saint Petersburg, Russia, 1923 June 2016.

Functional Plant Biology - https://doi.org/10.1071/FP16418
Submitted: 28 November 2016  Accepted: 3 April 2017   Published online: 17 May 2017

Abstract

In plant cells, peroxisomes participate in the metabolism of reactive oxygen species (ROS). One of the major regulators of cellular ROS levels – catalase (CAT) – occurs exclusively in peroxisomes. CAT activity is required for immunity-triggered autophagic programmed cell death (PCD). Autophagy has been recently demonstrated to represent a route for degradation of peroxisomes in plant cells. In the present study, the dynamics of the cellular peroxisome pool in tobacco BY-2 cell suspension cultures were used to analyse the effects of inhibition of basal autophagy with special attention to CAT activity. Numbers of peroxisomes per cell, levels of CAT protein and activity, cell viability, ROS levels and expression levels of genes encoding components of antioxidant system were analysed upon application of 3-methyladenine (3-MA), an inhibitor of autophagy, and/or aminotriazole (AT), an inhibitor of CAT. When applied separately, 3-MA and AT led to an increase in cell death, but this effect was attenuated by their simultaneous application. The obtained data suggest that both the levels of CAT protein in peroxisomes as well as CAT activity modulate the onset of cell death in tobacco BY-2 cells via ROS levels and autophagy.

Additional keywords: aminotriazole; 3-methyladenine.


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