Functional Plant Biology Functional Plant Biology Society
Plant function and evolutionary biology
RESEARCH ARTICLE

Myotubularins, PtdIns5P, and ROS in ABA-mediated stomatal movements in dehydrated Arabidopsis seedlings

Akanksha Nagpal A , Ammar Hassan A , Ivan Ndamukong B C , Zoya Avramova B D and František Baluška A D
+ Author Affiliations
- Author Affiliations

A IZMB, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany.

B School of Biological Sciences, UNL, Lincoln, NE 68588-6008, USA.

C Chowan University, Murfreesboro, NC 27855, USA.

D Corresponding authors. Emails: zavramova2@unl.edu; baluska@uni-bonn.de

This paper originates from a presentation at the Fourth International Symposium on Plant Signaling and Behavior, Komarov Botanical Institute RAS/Russian Science Foundation, Saint Petersburg, Russia, 1923 June 2016.

Functional Plant Biology - https://doi.org/10.1071/FP17116
Submitted: 18 April 2017  Accepted: 23 May 2017   Published online: 29 June 2017

Abstract

Myotubularins (MTMs) are lipid phosphoinositide 3-phosphate phosphatases and the product of their enzyme activity – phosphoinositide 5-phosphate (PtdIns5P) – functions as a signalling molecule in pathways involved in membrane dynamics and cell signalling. Two Arabidopsis genes, AtMTM1 and AtMTM2, encode enzymatically active phosphatases but although AtMTM1 deficiency results in increased tolerance to dehydration stress and a decrease in cellular PtdIns5P, the role of AtMTM2 is less clear, as it does not contribute to the PtdIns5P pool upon dehydration stress. Here we analysed the involvement of AtMTM1, AtMTM2 and PtdIns5P in the response of Arabidopsis seedlings to dehydration stress/ABA, and found that both AtMTM1 and AtMTM2 were involved but affected oppositely stomata movement and the accumulation of reactive oxygen species (ROS, e.g. H2O2). Acting as a secondary messenger in the ABA-induced ROS production in guard cells, PtdIns5P emerges as an evolutionarily conserved signalling molecule that calibrates cellular ROS under stress. We propose the biological relevance of the counteracting AtMTM1 and AtMTM2 activities is to balance the ABA-induced ROS accumulation and cellular homeostasis under dehydration stress.

Additional keywords: drought stress, guard cells, PI5P, stomata.


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