Culture of Protoplasts From Grape Vine Pericarp Callus

Abstract

Protoplasts isolated from callus cultures of the pericarp of Vitis vinifera cv. Sultana regenerated cell walls within 3 days, when cultured in semi-solid media adjusted to 0.4-0.5 osmolal. Up to 12% of the cells divided during the next 7 days. A lower proportion passed through two division cycles.