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Systematics, phylogeny and biogeography
RESEARCH ARTICLE

Systematics of the genus Halgerda Bergh, 1880 (Heterobranchia : Nudibranchia) of Mozambique with descriptions of six new species

Yara Tibiriçá A B E , Marta Pola C and Juan Lucas Cervera B D
+ Author Affiliations
- Author Affiliations

A SeaLife Station, Zavora Beach s/n, Inharrime, 1114, Inhambane Province, Mozambique.

B Departamento de Biología, Facultad de Ciencias del Mar y Ambientales; Campus de Excelencia Internacional del Mar (CEI·MAR), Av. República Saharaui s/n, 11510 Puerto Real (Cádiz), Spain.

C Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid; Campus de Excelencia Internacional UAM+CSIC, C/Darwin, 2, 28049 Madrid, Spain.

D Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEI·MAR) Universidad de Cádiz, Av. República Saharaui s/n, Ap. 40. 11510 Puerto Real (Cádiz), Spain.

E Corresponding author. Email: yara@zavoralab.com

Invertebrate Systematics 32(6) 1388-1421 https://doi.org/10.1071/IS17095
Submitted: 21 December 2017  Accepted: 4 July 2018   Published: 11 December 2018

Abstract

The species of the genus Halgerda Bergh, 1880, are restricted to the Indo-Pacific; some being common inhabitants of reefs off the coast of Mozambique. These species have been relatively well studied morphologically, but few molecular data are available. During a seven-year period surveying the reefs of Mozambique, 11 Halgerda spp. were collected, six of which are described here. We provide details on their morphology, anatomy, novel genetic markers and additional information about their colour variation. The new species described herein are Halgerda leopardalis, sp. nov., H. mozambiquensis, sp. nov., H. jennyae, sp. nov., H. meringuecitrea, sp. nov., H. nuarroensis, sp. nov. and H. indotessellata, sp. nov., the last of which was found to be a pseudocryptic species of H. tessellata. Moreover, we identified two species complexes, one composed mainly of specimens from the Western Indian Ocean and another with specimens mostly from the Pacific Ocean and Western Australia.

Additional keywords: biodiversity, DNA barcoding, Indo-Pacific, Mollusca, species delineation.


References

Akaike, H. (1974). A new look at the statistical model identification. IEEE Transactions on Automatic Control 19, 716–723.
A new look at the statistical model identification.Crossref | GoogleScholarGoogle Scholar |

Altschul, S. F., Gish, W., Miller, W., Myers, E. W., and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology 215, 403–410.
Basic local alignment search tool.Crossref | GoogleScholarGoogle Scholar |

Bergh, L. S. R. (1880). Malacologische Untersuchungen. In ‘Archipel der Philippinen. Zweiter Theil. Wissenschaftliche Resultate. Theil 4’. (Ed. C. Semper.) pp. 1–78. (Kreidel’s Verlag: Wiesbaden, Germany)

Bergh, L. S. R. (1888). Malacologische Untersuchungen. In ‘Reisen im Archipel der Philippinen von Dr. Carl Gottfried Semper. Zweiter Theil. Wissenschaftliche Resultate. Theil 3, Heft 16, 1 Hälfte’. pp. 755–814, pls. 77–81.

Carlson, C. H., and Hoff, P. J. (1993). Three new Halgerda species (Doridoidea: Nudibranchia: Opisthobranchia) from Guam. The Veliger 36, 16–26.

Carlson, C. H., and Hoff, P. J. (2000). Three new Pacific species of Halgerda (Opisthobranchia: Nudibranchia: Doridoidea). The Veliger 43, 154–163.

Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology and Evolution 17, 540–552.
Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis.Crossref | GoogleScholarGoogle Scholar |

Cheney, K. L., Cortesi, F., How, M. J., Wilson, N. G., Blomberg, S. P., Winters, A. E., Umanzör, S., and Marshall, N. J. (2014). Conspicuous visual signals do not coevolve with increased body size in marine sea slugs. Journal of Evolutionary Biology 27, 676–687.

Coleman, N. (2008). ‘Nudibranchs Encyclopedia.’ (Neville Coleman’s Underwater Geographic: Springwood, Qld, Australia.)

Colgan, D. J., Ponder, W. F., and Eggler, P. E. (2000). Gastropod evolutionary rates and phylogenetic relationships assessed using partial 28S rDNA and histone H3 sequences. Zoologica Scripta 29, 29–63.
Gastropod evolutionary rates and phylogenetic relationships assessed using partial 28S rDNA and histone H3 sequences.Crossref | GoogleScholarGoogle Scholar |

Cooke, S., Hanson, D., Hirano, Y., Ornelas-Gatdula, E., Gosliner, T. M., Chernyshev, A. V., and Valdés, Á. (2014). Cryptic diversity of Melanochlamys sea slugs (Gastropoda, Aglajidae) in the North Pacific. Zoologica Scripta 43, 351–369.
Cryptic diversity of Melanochlamys sea slugs (Gastropoda, Aglajidae) in the North Pacific.Crossref | GoogleScholarGoogle Scholar |

Debelius, H., and Kuiter, R. H. (2007). ‘Nudibranchs of the World.’ (IKAN-Unterwasserarchiv: Frankfurt, Germany.)

Dinapoli, A., Tamer, C., Franssen, S., Naduvilezhath, L., and Klussmann-Kolb, A. (2006). Utility of H3-gene sequences for phylogenetic reconstruction – a case study of heterobranch Gastropoda. Bonner Zoologische Beitrage 55, 191–202.

Edgar, R. C. (2004). MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32, 1792–1797.
MUSCLE: multiple sequence alignment with high accuracy and high throughput.Crossref | GoogleScholarGoogle Scholar |

Eliot, C. N. E. (1904). On some nudibranchs from East Africa and Zanzibar. Part IV. Proceedings of the Zoological Society of London 1904, 380–406.
On some nudibranchs from East Africa and Zanzibar. Part IV.Crossref | GoogleScholarGoogle Scholar |

Eliot, C. N. E. (1905). On some nudibranchs from East Africa and Zanzibar. Part VI. Proceedings of the Zoological Society of London 1904, 268–298.

Fahey, S. J. (2003). Phylogeny of Halgerda (Mollusca: Gastropoda) based on combined analysis of mitochondrial CO1 and morphology. Invertebrate Systematics 17, 617–624.
Phylogeny of Halgerda (Mollusca: Gastropoda) based on combined analysis of mitochondrial CO1 and morphology.Crossref | GoogleScholarGoogle Scholar |

Fahey, S. J., and Carroll, A. R. (2007). Natural products isolated from species of Halgerda Bergh, 1880 (Mollusca: Nudibranchia) and their ecological and evolutionary implications. Journal of Chemical Ecology 33, 1226–1234.
Natural products isolated from species of Halgerda Bergh, 1880 (Mollusca: Nudibranchia) and their ecological and evolutionary implications.Crossref | GoogleScholarGoogle Scholar |

Fahey, S. J., and Gosliner, T. M. (1999a). Description of three new species of Halgerda from the Western Indian Ocean with a redescription of Halgerda formosa, Bergh 1880. Proceeding of the California Academy of Sciences 51, 365–383.

Fahey, S. J., and Gosliner, T. M. (1999b). Preliminary phylogeny of Halgerda (Nudibranchia: Halgeridae) from the Tropical Indo-Pacific, with descriptions of three new species. Proceeding of the California Academy of Sciences 51, 425–448.

Fahey, S. J., and Gosliner, T. M. (2000). New records of Halgerda Bergh, 1880 (Opisthobranchia, Nudibranchia) from the deep western Pacific Ocean, with descriptions of four new species. Zoosystematics and Evolution 22, 471–498.

Fahey, S. J., and Gosliner, T. M. (2001a). On the genus Halgerda (Nudibranchia: Halgerdidae) from Western Australia with descriptions of four new species. Bollettino Malacologico 37, 55–76.

Fahey, S. J., and Gosliner, T. M. (2001b). The phylogeny of Halgerda (Opisthobranchia, Nudibranchia) with the description of a new species from Okinawa. Zoologica Scripta 30, 199–213.
The phylogeny of Halgerda (Opisthobranchia, Nudibranchia) with the description of a new species from Okinawa.Crossref | GoogleScholarGoogle Scholar |

Fahey, S. J., and Gosliner, T. M. (2003). Redescription of Halgerda graphica Basedow and Hedley, 1905, with observations on external morphological variation within selected species of Halgerda (Mollusca: Nudibranchia). Proceedings of the California Academy of Sciences 54, 393–406.
Redescription of Halgerda graphica Basedow and Hedley, 1905, with observations on external morphological variation within selected species of Halgerda (Mollusca: Nudibranchia).Crossref | GoogleScholarGoogle Scholar |

Fahey, S. J., and Healy, J. M. (2003). Sperm ultrastructure in the nudibranch genus Halgerda with reference to other Discodorididae and to Chromodorididae (Mollusca : Opisthobranchia). Journal of Morphology 257, 9–21.
Sperm ultrastructure in the nudibranch genus Halgerda with reference to other Discodorididae and to Chromodorididae (Mollusca : Opisthobranchia).Crossref | GoogleScholarGoogle Scholar |

Farran, G. P. (1905). Report on the opisthobranchiate Mollusca collected by Professor Herdman, at Ceylon, in 1902. Report to the Government of Ceylon on the Pearl Oyster Fisheries of the Gulf of Manaar 20, 329–364.

Fisher-Reid, M. C., and Wiens, J. J. (2011). What are the consequences of combining nuclear and mitochondrial data for phylogenetic analysis? Lessons from Plethodon salamanders and 13 other vertebrate clades. BMC Evolutionary Biology 11, 300.
What are the consequences of combining nuclear and mitochondrial data for phylogenetic analysis? Lessons from Plethodon salamanders and 13 other vertebrate clades.Crossref | GoogleScholarGoogle Scholar |

Folmer, O., Black, M., Hoeh, W., Lutz, R., and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxydase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3, 294–299.

Giribet, G., Okusu, A., Lindgren, A. R., Huff, S. W., Schrödl, M., and Nishiguchi, M. K. (2006). Evidence for a clade composed of molluscs with serially repeated structures: monoplacophorans are related to chitons. Proceedings of the National Academy of Sciences 103, 7723–7728.
Evidence for a clade composed of molluscs with serially repeated structures: monoplacophorans are related to chitons.Crossref | GoogleScholarGoogle Scholar |

Göbbeler, K., and Klussmann-kolb, A. (2010). Out of Antarctica?–New insights into the phylogeny and biogeography of the Pleurobranchomorpha (Mollusca, Gastropoda). Molecular Phylogenetics and Evolution 55, 996–1007.
Out of Antarctica?–New insights into the phylogeny and biogeography of the Pleurobranchomorpha (Mollusca, Gastropoda).Crossref | GoogleScholarGoogle Scholar |

Gosliner, T. M. (1987). ‘Nudibranchs of southern Africa: a guide to opisthobranch molluscs of southern Africa.’ (Sea Challengers, California Academy of Sciences: San Francisco, CA, USA.)

Gosliner, T. M., and Fahey, S. J. (1998). Description of a new species of Halgerda from the Indo-Pacific with a redescription of Halgerda elegans Bergh, 1905. Proceeding of the California Academy of Sciences 50, 347–359.

Gosliner, T. M., Behrens, D. and Valdés, Á. (2008). ‘Indo-Pacific nudibranchs and sea slugs: a field guide to the world’s most diverse fauna.’ (Sea Challengers, California Academy of Sciences: San Francisco, CA, USA.)

Hickerson, M. J., Meyer, C. P., and Moritz, C. (2006). DNA barcoding will often fail to discover new animal species over broad parameter space. Systematic Biology 55, 729–739.
DNA barcoding will often fail to discover new animal species over broad parameter space.Crossref | GoogleScholarGoogle Scholar |

Hubert, N., and Hanner, R. (2015). DNA barcoding, species delineation and taxonomy: a historical perspective. DNA Barcodes 3, 44–58.
DNA barcoding, species delineation and taxonomy: a historical perspective.Crossref | GoogleScholarGoogle Scholar |

Jörger, K. M., and Schrödl, M. (2013). How to describe a cryptic species? Practical challenges of molecular taxonomy. Frontiers in Zoology 10, 59.

Jörger, K. M., and Schrödl, M. (2014). How to use CAOS software for taxonomy? A quick guide to extract diagnostic nucleotides or amino acids for species descriptions. Spixiana 37, 21–26.

Jörger, K. M., Norenburg, J. L., Wilson, N. G., and Schrödl, M. (2012). Barcoding against a paradox? Combined molecular species delineations reveal multiple cryptic lineages in elusive meiofaunal sea slugs. BMC Evolutionary Biology 12, 245.
Barcoding against a paradox? Combined molecular species delineations reveal multiple cryptic lineages in elusive meiofaunal sea slugs.Crossref | GoogleScholarGoogle Scholar |

Kashiwagi, T., Marshall, A. D., Bennett, M. B., and Ovenden, J. R. (2012). The genetic signature of recent speciation in manta rays (Manta alfredi and M. birostris). Molecular Phylogenetics and Evolution 64, 212–218.
The genetic signature of recent speciation in manta rays (Manta alfredi and M. birostris).Crossref | GoogleScholarGoogle Scholar |

Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., and Thierer, T. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28, 1647–1649.
Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data.Crossref | GoogleScholarGoogle Scholar |

Kekkonen, M., Mutaren, M., Kaila, L., Nieminen, M., and Hebert, P. D. N. (2015). Delineating species with DNA barcodes: a case of taxon dependent method performance in moths. PlosOne 10, e0122481.

Lindsay, T., and Valdés, Á. (2016). The model organism Hermissenda crassicornis (Gastropoda: Heterobranchia) is a species complex. PLoS One 11, e0154265.
The model organism Hermissenda crassicornis (Gastropoda: Heterobranchia) is a species complex.Crossref | GoogleScholarGoogle Scholar |

Mahguib, J., and Valdés, Á. (2015). Molecular investigation of the phylogenetic position of the polar nudibranch Doridoxa (Mollusca, Gastropoda, Heterobranchia). Polar Biology 38, 1369–1377.
Molecular investigation of the phylogenetic position of the polar nudibranch Doridoxa (Mollusca, Gastropoda, Heterobranchia).Crossref | GoogleScholarGoogle Scholar |

Malaquias, M., Calado, G., Padula, V., Villani, G., and Cervera, J. L. (2009). Molluscan diversity in the North Atlantic Ocean: new records of opisthobranch gastropods from the Archipelago of the Azores. Marine Biodiversity Records 2, e38.
Molluscan diversity in the North Atlantic Ocean: new records of opisthobranch gastropods from the Archipelago of the Azores.Crossref | GoogleScholarGoogle Scholar |

Matsuda, S. B., and Gosliner, T. M. (2018). Molecular phylogeny of Glossodoris (Ehrenberg, 1831) nudibranchs and related genera reveals cryptic and pseudocryptic species complexes. Cladistics 34, 41–56.
Molecular phylogeny of Glossodoris (Ehrenberg, 1831) nudibranchs and related genera reveals cryptic and pseudocryptic species complexes.Crossref | GoogleScholarGoogle Scholar |

Meier, R., Shiyang, W., Viada, G., and Ng, P. K. L. (2006). DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. Systematics Biology 55, 715–728.

Meier, R., Zhang, G., and Ali, F. (2008). The use of mean instead of smallest interspecific distances exaggerates the size of the ‘barcoding gap’ and leads to misidentification. Systematic Biology 57, 809–813.
The use of mean instead of smallest interspecific distances exaggerates the size of the ‘barcoding gap’ and leads to misidentification.Crossref | GoogleScholarGoogle Scholar |

Moritz, C., and Cicero, C. (2004). DNA barcoding: promise and pitfalls. PLoS Biology 2, e354.
DNA barcoding: promise and pitfalls.Crossref | GoogleScholarGoogle Scholar |

Müller, J., and Müller, K. (2004). TreeGraph: automated drawing of complex tree figures using an extensible tree description format. Molecular Ecology Resources 4, 786–788.
TreeGraph: automated drawing of complex tree figures using an extensible tree description format.Crossref | GoogleScholarGoogle Scholar |

Nylander, J. A., Ronquist, F., Huelsenbeck, J. P., and Nieves-Aldrey, J. L. (2004). Bayesian phylogenetic analysis of combined data. Systematic Biology 53, 47–67.
Bayesian phylogenetic analysis of combined data.Crossref | GoogleScholarGoogle Scholar |

Ortigosa, D., Pola, M., Carmona, L., Padula, V., Schrödl, M., and Cervera, J. L. (2014). Redescription of Felimida elegantula (Philippi, 1844) and a preliminary phylogeny of the European species of Felimida (Chromodorididae). The Journal of Molluscan Studies 80, 541–550.
Redescription of Felimida elegantula (Philippi, 1844) and a preliminary phylogeny of the European species of Felimida (Chromodorididae).Crossref | GoogleScholarGoogle Scholar |

Palumbi, S. R. (1994). Genetic divergence, reproductive isolation, and marine speciation. Annual Review of Ecology and Systematics 25, 547–572.

Pereira, M., Litulo, R., Santos, R., Leal, M., Fernandes, R. S., Tibiriçá, Y., Williams, J., Atanassov, B., Carreira, F., Massinge, A., and Silva, I. M. (2014). ‘Mozambique Marine Ecosystems Review. Foundation Ensemble.’ (Biodinamica/CTV: Maputo, Mozambique.)

Pola, M., Camacho-García, Y. E., and Gosliner, T. M. (2012). Molecular data illuminate cryptic nudibranch species: the evolution of the Scyllaeidae (Nudibranchia: Dendronotina) with a revision of Notobryon. Zoological Journal of the Linnean Society 165, 311–336.
Molecular data illuminate cryptic nudibranch species: the evolution of the Scyllaeidae (Nudibranchia: Dendronotina) with a revision of Notobryon.Crossref | GoogleScholarGoogle Scholar |

Pola, M., Padula, V., Gosliner, T. M., and Cervera, J. L. (2014a). Going further on an intricate and challenging group of nudibranchs: description of five novel species and a more complete molecular phylogeny of the subfamily Nembrothinae (Polyceridae). Cladistics 30, 607–634.
Going further on an intricate and challenging group of nudibranchs: description of five novel species and a more complete molecular phylogeny of the subfamily Nembrothinae (Polyceridae).Crossref | GoogleScholarGoogle Scholar |

Pola, M., Roldan, P., and Padilla, S. (2014b). Molecular data on the genus Okenia (Nudibranchia: Goniodorididae) reveal a new cryptic species from New South Wales (Australia). Journal of the Marine Biological Association of the United Kingdom 94, 587–598.
Molecular data on the genus Okenia (Nudibranchia: Goniodorididae) reveal a new cryptic species from New South Wales (Australia).Crossref | GoogleScholarGoogle Scholar |

Puillandre, N., Lambert, A., Brouillet, S., and Achaz, G. (2012). ABGD, Automatic Barcode Gap Discovery for primary species delimitation. Molecular Ecology 21, 1864–1877.
ABGD, Automatic Barcode Gap Discovery for primary species delimitation.Crossref | GoogleScholarGoogle Scholar |

Ronquist, F., and Huelsenbeck, J. P. (2003). MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.
MRBAYES 3: Bayesian phylogenetic inference under mixed models.Crossref | GoogleScholarGoogle Scholar |

Rudman, W. B. (1978). The dorid opisthobranch genera Halgerda Bergh and Sclerodoris Eliot from the Indo-West. Zoological Journal of the Linnean Society 62, 59–88.
The dorid opisthobranch genera Halgerda Bergh and Sclerodoris Eliot from the Indo-West.Crossref | GoogleScholarGoogle Scholar |

Rudman, W. B. (2001). Halgerda punctata Farran, 1905. Sea Slug Forum. Australian Museum, Sydney. Available at: http://www.seaslugforum.net/find/halgpunc [Accessed 31 May 2017].

Rudman, W. B. (2009). Comment on Re: Halgerda carlsoni from East Africa by Sully et Monika Bachel. Sea Slug Forum, Australian Museum, Sydney. Available at: http://www.seaslugforum.net/find/22233 [Accessed 20 May 2017].

Satler, J. D., Cartens, B. C., and Hedin, M. (2013). Multilocus species delimitation in a complex of morphologically conserved trapdoor spiders (Mygalomorphae, Antrodiaetidae, Aliatypus). Systematic Biology 62, 805–823.
Multilocus species delimitation in a complex of morphologically conserved trapdoor spiders (Mygalomorphae, Antrodiaetidae, Aliatypus).Crossref | GoogleScholarGoogle Scholar |

Stamatakis, A. (2014). RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30, 1312–1313.
RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies.Crossref | GoogleScholarGoogle Scholar |

Sun, S., Li, Q., Kong, L., Yu, H., Zheng, X., Yu, R., Dai, L., Sun, Y., Chen, J., Liu, J., Ni, L., Feng, Y., Yu, Z., Zou, S., and Lin, J. (2016). DNA barcoding reveal patterns of species diversity among northwestern Pacific molluscs. Scientific Reports 6, 33367.
DNA barcoding reveal patterns of species diversity among northwestern Pacific molluscs.Crossref | GoogleScholarGoogle Scholar |

Tibiriçá, Y., and Malaquias, M. A. E. (2017). The bubble snails (Gastropoda, Heterobranchia) of Mozambique: an overlooked biodiversity hotspot. Marine Biodiversity 47, 791–811.
The bubble snails (Gastropoda, Heterobranchia) of Mozambique: an overlooked biodiversity hotspot.Crossref | GoogleScholarGoogle Scholar |

Tibiriçá, Y., Pola, M., and Cervera, J. L. (2017). Astonishing diversity revealed: an annotated and illustrated inventory of Nudipleura (Gastropoda: Heterobranchia) from Mozambique. Zootaxa 4359, 1–133.
Astonishing diversity revealed: an annotated and illustrated inventory of Nudipleura (Gastropoda: Heterobranchia) from Mozambique.Crossref | GoogleScholarGoogle Scholar |

Valdés, Á. (2002). A phylogenetic analysis and systematic revision of the cryptobranch dorids (Mollusca, Nudibranchia, Anthobranchia). Zoological Journal of the Linnean Society 136, 535–636.
A phylogenetic analysis and systematic revision of the cryptobranch dorids (Mollusca, Nudibranchia, Anthobranchia).Crossref | GoogleScholarGoogle Scholar |

Wiemers, M., and Fiedler, K. (2007). Does the DNA barcoding gap exist? A case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology 4, 8.
Does the DNA barcoding gap exist? A case study in blue butterflies (Lepidoptera: Lycaenidae).Crossref | GoogleScholarGoogle Scholar |

Wilson, N., and Kirkendale, L. (2016). Putting the ‘Indo’ back into the Indo-Pacific: Resolving marine phylogeographic gaps. Invertebrate Systematics 30, 86–94.
Putting the ‘Indo’ back into the Indo-Pacific: Resolving marine phylogeographic gaps.Crossref | GoogleScholarGoogle Scholar |

Xi, Z., Liu, L., and Davis, C. C. (2015). The impact of missing data on species tree estimation. Molecular Biology and Evolution 33, 838–860.

Xia, Y., Gu, H., Peng, R., Chen, Q., Zheng, Y., Murphy, R. W., and Zeng, X. (2012). COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae). Molecular Ecology Resources 12, 48–56.
COI is better than 16S rRNA for DNA barcoding Asiatic salamanders (Amphibia: Caudata: Hynobiidae).Crossref | GoogleScholarGoogle Scholar |

Yonow, N. (2012). Opisthobranchs from the western Indian Ocean, with descriptions of two new species and ten new records (Mollusca, Gastropoda). ZooKeys 197, 1–129.
Opisthobranchs from the western Indian Ocean, with descriptions of two new species and ten new records (Mollusca, Gastropoda).Crossref | GoogleScholarGoogle Scholar |

Yonow, N., Anderson, R. C., and Buttress, S. G. (2002). Opisthobranch molluscs from the Chagos Archipelago, Central Indian Ocean. Journal of Natural History 36, 831–882.
Opisthobranch molluscs from the Chagos Archipelago, Central Indian Ocean.Crossref | GoogleScholarGoogle Scholar |

Zhang, C., Zhang, D. X., Zhu, T., and Yang, Z. (2011). Evaluation of a Bayesian coalescent method of species delimitation. Systematic Biology 60, 747–761.
Evaluation of a Bayesian coalescent method of species delimitation.Crossref | GoogleScholarGoogle Scholar |

Zhang, J., Kapli, P., Pavlidis, P., and Stamatakis, A. (2013). A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29, 2869–2876.
A general species delimitation method with applications to phylogenetic placements.Crossref | GoogleScholarGoogle Scholar |