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Exploring the diversity of Asian Cryptocercus (Blattodea: Cryptocercidae): species delimitation based on chromosome numbers, morphology, and molecular analysis
Woodroaches from the genus Cryptocercus are known to display low levels of morphological divergence, yet significant genetic divergence and variability in chromosome number. Compared with Cryptocercus taxa from North America, the diversity of the genus in Asia has received relatively little attention. We performed morphological and karyotypic examinations of multiple taxa from a number of never-before sampled mountainous areas of Central and southwestern China, and identified 9 candidate species primarily on the basis of chromosome number. We then investigated diversity across all Asian Cryptocercus, through phylogenetic analyses of 135 COI sequences and 74 28S rRNA sequences from individuals of 28 localities, including species delimitation analysis in General Mixed Yule Coalescent (GMYC) and Automatic Barcode Gap Discovery (ABGD). Phylogenetic results indicated that individuals from the same locality constituted well-supported clades. The congruence of GMYC and ABGD results were in almost perfect accord with 28 candidate species described on the basis of karyotypes (including the 9 identified in this study). We provide evidence that each valley population in the Hengduan Mountains contains a separate evolving lineage. We conclude that the principal cause of the rich Cryptocercus diversity in China has been uplift of the Qinghai-Tibet Plateau.
IS17003 Accepted 01 June 2017
© CSIRO 2017