Marine and Freshwater Research Marine and Freshwater Research Society
Advances in the aquatic sciences
RESEARCH ARTICLE

Nitrogen removal during the cold season by constructed floating wetlands planted with Oenanthe javanica

Penghe Wang A B , Nasreen Jeelani A B , Jie Zuo A , Hui Zhang A , Dehua Zhao A B D , Zhengjie Zhu A C , Xin Leng A B D and Shuqing An A B
+ Author Affiliations
- Author Affiliations

A Institute of Wetland Ecology, School of Life Science, Nanjing University, Xianlin Avenue 163, Nanjing 210046, P.R. China.

B Nanjing University Ecology Research Institute of Changshu, Huanhu Road 1, Changshu 215500, P.R. China.

C College of Oceanography, Hohai University, Xikang Road 1, Nanjing 210098, P.R. China.

D Corresponding authors. Email: dhzhao@nju.edu.cn; lengx@nju.edu.cn

Marine and Freshwater Research - https://doi.org/10.1071/MF17156
Submitted: 30 May 2017  Accepted: 7 August 2017   Published online: 26 September 2017

Abstract

Constructed floating wetlands (CFWs) are used to treat waste waters of various origins either alone or as part of waste water treatment trains. The aim of the present study was to determine the extent of nitrogen removal by CFWs planted with Oenanthe javanica (Blume) DC. at low temperatures (<10°C) and whether CFWs with vesuvianite as a substrate perform better than those without substrate. A batch model was used, with CFWs planted with O. javanica (Tc), CFWs without O. javanica (Ts), CFWs without substrate (Tp) and floating mats only (To) as a control. The average removal rates of NH4+-N, NO3-N and total nitrogen were 78.3, 44.4 and 49.7% respectively in Tc; 72.0, 40.0 and 39.5% respectively in Ts; and 73.1, 33.7 and 44.0% respectively in Tp. In addition to a gradual increase in chemical oxygen demand during the experimental period, Tc had higher microbial richness and diversity, as well as a higher abundance of bacteria, archaea, anaerobic ammonium oxidation (Anammox) bacteria and key genes (ammonia mono-oxygenase, amoA, nitrous oxide reductase, nosZ, dissimilatory cd1-containing nitrite reductase, nirS, and dissimilatory copper-containing nitrite reductase, nirK) involved in nitrogen metabolism in the substrate than Ts. Further analysis of microbial community composition revealed a difference at multiple taxonomic levels among different systems. These results demonstrate the positive roles of O. javanica and vesuvianite in CFWs in nitrogen removal from waste water during the cold season (mean water temperature <10°C).

Additional keywords: C : N ratio, carbon source, gene abundance, nitrification–denitrification, rhizoplane micro-organism.


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